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Dominant changes in the breast muscle lipid profiles of broiler chickens with wooden breast syndrome revealed by lipidomics analyses

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1703-1713页

作者： Ranran Liu Fuli Kong Siyuan Xing Zhengxiao He Lu Bai jiahong sun Xiaodong Tan Di Zhao Guiping Zhao Jie Wen Institute of Animal Sciences Chinese Academy of Agricultural SciencesState Key Laboratory of Animal NutritionKey Laboratory of Animal(Poultry)Genetics Breeding and ReproductionMinistry of AgricultureBeijingChina

Background: Chicken is the most consumed meat worldwide and the industry has been facing challenging myopathies. Wooden breast(WB), which is often accompanied by white striping(WS), is a serious myopathy adversely affecting meat quality of breast muscles. The underlying lipid metabolic mechanism of WB affected broilers is not fully ***: A total of 150 chickens of a white-feathered, fast-growing pure line were raised and used for the selection of WB, WB + WS and control chickens. The lipids of the breast muscle, liver, and serum from different chickens were extracted and measured using ultra performance liquid chromatography(UPLC) plus Q-Exactive Orbitrap tandem mass spectrometry. In the breast, 560 lipid molecules were identified. Compared to controls, 225/225 of 560 lipid molecules(40.2%) were identified with differential abundance(DA), including 92/100 significantly increased neutral lipids and 107/98 decreased phospholipids in the WB/WB + WS groups, respectively. The content of monounsaturated fatty acids(MUFA) was significantly higher, and the polyunsaturated fatty acids(PUFA) and saturated fatty acids(SFA) were significantly lower in the affected breasts. In the liver, 434 lipid molecules were identified, and 39/61 DA lipid molecules(6.7%/14.1%) were detected in the WB and WB + WS groups, respectively. In the serum, a total of 529 lipid molecules were identified and 4/44 DA lipid molecules(0.8%/8.3%) were detected in WB and WB + WS group, respectively. Compared to controls, the content of MUFAs in the serum and breast of the WB + WS group were both significantly increased, and the content of SFAs in two tissues were both significantly decreased. Only five lipid molecules were consistently increased in both liver and serum in WB + WS ***: We have found for the first time that the dominant lipid profile alterations occurred in the affected breast muscle. The relative abundance of 40.2% of lipid molecules were changed and is characteri

关键词： Broiler chicken Lipid profle Liver Serum Wooden breast

Lipidomics reveals carnitine palmitoyltransferase 1C protects cancer cells from lipotoxicity and senescence

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Journal of Pharmaceutical Analysis 2021年第3期11卷 340-350页

作者： Huizhen Zhang Yongtao Wang Lihuan Guan Yixin Chen Panpan Chen jiahong sun Frank J.Gonzalez Min Huang Huichang Bi Guangdong Provincial Key Laboratory of New Drug Design and Evaluation School of Pharmaceutical SciencesSun Yat-Sen UniversityGuangzhou510006China Laboratory of Metabolism Center for Cancer ResearchNational Cancer InstituteNational Institutes of HealthBethesdaMD20892USA

Lipotoxicity, caused by intracellular lipid accumulation, accelerates the degenerative process of cellular senescence, which has implications in cancer development and therapy. Previously, carnitine palmitoyltransferase 1C(CPT1C), a mitochondrial enzyme that catalyzes carnitinylation of fatty acids, was found to be a critical regulator of cancer cell senescence. However, whether loss of CPT1C could induce senescence as a result of lipotoxicity remains unknown. An LC/MS-based lipidomic analysis of PANC-1,MDA-MB-231, HCT-116 and A549 cancer cells was conducted after siRNA depletion of CPT1C. Cellular lipotoxicity was further confirmed by lipotoxicity assays. Significant changes were found in the lipidome of CPT1C-depleted cells, including major alterations in fatty acid, diacylglycerol, triacylglycerol, oxidative lipids, cardiolipin, phosphatidylglycerol, phosphatidylcholine/phosphatidylethanolamine ratio and sphingomyelin. This was coincident with changes in expressions of mRNAs involved in *** and biochemical analyses revealed higher lipid accumulation and increased malondialdehyde and reactive oxygen species, signatures of lipid peroxidation and oxidative stress. Reduction of ATP synthesis, loss of mitochondrial transmembrane potential and down-regulation of expression of mitochondriogenesis gene m RNAs indicated mitochondrial dysfunction induced by lipotoxicity, which could further result in cellular senescence. Taken together, this study demonstrated CPT1C plays a critical role in the regulation of cancer cell lipotoxicity and cell senescence, suggesting that inhibition of CPT1C may serve as a new therapeutic strategy through induction of tumor lipotoxicity and senescence.

关键词： Lipidomics Lipid accumulation Lipid peroxidation Oxidative stress Mitochondrial dysfunction Anticancer target

SIRT6 as a key event linking P53 and NRF2 counteracts APAP-induced hepatotoxicity through inhibiting oxidative stress and promoting hepatocyte proliferation

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Acta Pharmaceutica Sinica B 2021年第1期11卷 89-99页

作者： Yanying Zhou Xiaomei Fan Tingying Jiao Wenzhou Li Panpan Chen Yiming Jiang jiahong sun Yixin Chen Pan Chen Lihuan Guan Yajie Wen Min Huang Huichang Bi School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China Shenzhen Baoan Women’s and Children’s Hospital Jinan UniversityShenzhen 518102China Department of Pharmacy the First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China

Acetaminophen(APAP)overdose is the leading cause of drug-induced liver injury,and its prognosis depends on the balance between hepatocyte death and *** 6(SIRT6)has been reported to protect against oxidative stress-associated DNA *** whether SIRT6 regulates APAPinduced hepatotoxicity remains *** this study,the protein expression of nuclear and total SIRT6 was up-regulated in mice liver at 6 and 48 h following APAP treatment,***6 knockdown in AML12 cells aggravated APAP-induced hepatocyte death and oxidative stress,inhibited cell viability and proliferation,and downregulated CCNA1,CCND1 and CKD4 protein ***6 knockdown signifi-cantly prevented APAP-induced NRF2 activation,reduced the transcriptional activities of GSTm andNQO1 and the m RNA levels of Nrf2,Ho-1,Gsta and ***,SIRT6 showed potential protein interaction with NRF2 as evidenced by co-immunoprecipitation(Co-IP)***,the protective effect of P53 against APAP-induced hepatocytes injury was *** Sirt6 m RNA was significantly down-regulated in P53à/àmice.P53 activated the transcriptional activity of SIRT6 and exerted interaction with *** results demonstrate that SIRT6 protects against APAP hepatotoxicity through alleviating oxidative stress and promoting hepatocyte proliferation,and provide new insights in the function of SIRT6 as a crucial docking molecule linking P53 and NRF2.

关键词： Acetaminophen Hepatotoxicity SIRT6 NRF2 P53

光绪三十二年章董氏《刑律草案》(稿本)所附签注之研究

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华东政法大学学报 2010年第4期13卷 111-122页

作者：孙家红北京大学

1911年,《大清新刑律》颁布,标志着中国刑法典诞生。百年来,我国刑法的曲折发展一直是学界关注的热点。2010年4月17日至18日,华中科技大学近代法研究所举办了"中国刑法典诞生100周年"的学术研讨会,对于清末修律以来中国的刑法体系、罪... 详细信息

1911年,《大清新刑律》颁布,标志着中国刑法典诞生。百年来,我国刑法的曲折发展一直是学界关注的热点。2010年4月17日至18日,华中科技大学近代法研究所举办了"中国刑法典诞生100周年"的学术研讨会,对于清末修律以来中国的刑法体系、罪名、刑事司法等方面的转变进行了集中交流。本期热点笔谈的三篇文章皆是提交于该研讨会的佳作,在此一并刊载,希翼借此及时展现此领域的最新研究成果,同时也有助于我们对于百年中国刑法史的反思。对于学界名家黄源盛教授,及孙家红、孔庆平两位博士的赐稿,及俞江教授的协助组稿,一并致以诚挚的谢意。

关键词：沈家本案语修律条文按语稿本

走近讼师秘本的世界——对夫马进《讼师秘本<萧曹遗笔>的出现》一文若干论点的驳论

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比较法研究 2008年第4期22卷 81-89页

作者：孙家红北京大学法学院博士研究生

讼师秘本和讼师进入法史学者的研究范围，也就是十几年的事。然而，说来惭愧，国内相关研究不仅起步较晚，而且研究的深度、广度均显不足。以致日本学者夫马进在他那篇著名的《讼师秘本〈萧曹遗笔〉的出现》（以下简称《出现》）一文中... 详细信息

讼师秘本和讼师进入法史学者的研究范围，也就是十几年的事。然而，说来惭愧，国内相关研究不仅起步较晚，而且研究的深度、广度均显不足。以致日本学者夫马进在他那篇著名的《讼师秘本〈萧曹遗笔〉的出现》（以下简称《出现》）一文中，不无骄傲地说：“关于讼师秘本的研究，就我所知，除了郑秦略为涉及外，其它无人论述”。近年来，国内有关讼师和讼师秘本的研究性著作和论文也陆续发表了若干种，研究水平却不够深入。

关键词：讼师驳论论点世界日本学者研究性国内法史

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域外偶得:一张珍贵的老北大图书馆照片

大学图书馆学报 2016年第1期34卷 108-111,95页

作者：孙家红中国社会科学院法学研究所北京100720

1912年6月14日,法国著名杂志《画报》的签约画家兼记者萨巴德来到北京大学参观,访问当时在华任教的几位法籍教授,并在北京大学图书馆的庭院中留下珍贵合影。这张合影不仅可以推定明确的摄影日期,而且与中华民国成立、京师大学堂改名北... 详细信息

1912年6月14日,法国著名杂志《画报》的签约画家兼记者萨巴德来到北京大学参观,访问当时在华任教的几位法籍教授,并在北京大学图书馆的庭院中留下珍贵合影。这张合影不仅可以推定明确的摄影日期,而且与中华民国成立、京师大学堂改名北京大学,皆在同年,因而十分难得。

关键词：萨巴德《画报》北大校史

遥对故人思故国——读梁漱溟、唐君毅两先生五封往来书信并纪念唐君毅先生逝世四十周年

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中国文化研究 2019年第3期 112-132页

作者：孙家红中国社会科学院法学研究所

梁漱溟和唐君毅是中国现代思想文化峰林中两座令人瞩目的学术高峰。现存梁、唐两先生往来书信一共五封,虽然数量十分有限,但为解读梁、唐二位先生间跨越半个多世纪的学术交谊,提供了难得的历史材料。本文利用新近出版的《梁漱溟往来书... 详细信息

梁漱溟和唐君毅是中国现代思想文化峰林中两座令人瞩目的学术高峰。现存梁、唐两先生往来书信一共五封,虽然数量十分有限,但为解读梁、唐二位先生间跨越半个多世纪的学术交谊,提供了难得的历史材料。本文利用新近出版的《梁漱溟往来书信集》《唐君毅全集》,重点结合梁、唐二位先生书信、日记等资料,对这五封书信的写作背景、所涉人物关系,以及相关内容思想,按照时代顺序,逐篇进行解读,不仅使读者之思想境界得到某种程度的激荡升华,更丰富了我们对于两位学术前辈学术观点、思绪情感、家国情怀之理解。

关键词：梁漱溟唐君毅新儒家

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略论唐律的“不孝罪”

中国文化研究 2007年第4期 40-51页

作者：孙家红龚汝富北京大学法学院 100871 江西财经大学法学院南昌330013

《唐律》立法精妙,"一准乎礼",对于"不孝罪",既列举有细致的原则性规定,又在具体司法实践中,区别对待,审慎抉择。虽然难免出现礼法原则之矛盾冲突,但也含有相当的实事求是、平等主义因素,不仅为后来诸朝的立法司法活动提供了成功经验,... 详细信息

《唐律》立法精妙,"一准乎礼",对于"不孝罪",既列举有细致的原则性规定,又在具体司法实践中,区别对待,审慎抉择。虽然难免出现礼法原则之矛盾冲突,但也含有相当的实事求是、平等主义因素,不仅为后来诸朝的立法司法活动提供了成功经验,更对中华传统家庭伦理的涵育养成产生了深远影响,值得今人重视。

关键词：唐律不孝罪儒家思想

历史是一条奔流不息的河——再评戴史翠《常规帝国的不确定性》引发的学术论争

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社会科学论坛 2024年第5期 196-224页

作者：孙家红中国社会科学院法学研究所北京100720

戴史翠的个人专著《常规帝国的不确定性》出版后,在海内外学界引起广泛争议。2023年9月乔志健发表长篇书评,之后相继有几位学者对之表达批评意见,并引起作者撰文回应,提出反批评。对于这场学术论争进行重新审视,针对彼此在专业知识、方... 详细信息

戴史翠的个人专著《常规帝国的不确定性》出版后,在海内外学界引起广泛争议。2023年9月乔志健发表长篇书评,之后相继有几位学者对之表达批评意见,并引起作者撰文回应,提出反批评。对于这场学术论争进行重新审视,针对彼此在专业知识、方法论等方面的巨大分歧,提出系统批评,并对戴书核心论证的“18世纪清朝的行政革命”,结合相关史料尝试提出不同的思考维度,冀收抛砖引玉之效。

关键词：常规帝国不确定性戴史翠乔志健书评