检索结果-南通市图书馆

Conjugated microporous polymers-scaffolded enzyme cascade systems with enhanced catalytic activity

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Catalysis 2024年第8期63卷 213-223页

作者： Zhenhua Wu jiafu Shi Boyu Zhang Yushuai Jiao Xiangxuan Meng Ziyi Chu Yu Chen Yiran Cheng Zhongyi jiang School of Environmental Science and Engineering Tianjin UniversityTianjin 300072China Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Haihe Laboratory of Sustainable Chemical Transformations Tianjin 300192China State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China College of Chemical Engineering Fuzhou UniversityFuzhou 350108FujianChina Joint School of National University of Singapore and Tianjin University International Campus of Tianjin UniversityFuzhou 350207FujianChina

Enhancing catalytic activity of multi-enzyme in vitro through substrate channeling effect is promis-ing yet ***,conjugated microporous polymers(CMPs)-scaffolded integrated en-zyme cascade systems(I-ECSs)are constructed through co-entrapping glucose oxidase(GOx)and horseradish peroxidase(HRP),in which hydrogen peroxide(H_(2)O_(2))is the intermediate *** interplay of low-resistance mass transfer pathway and appropriate pore wall-H_(2)O_(2)interactions facilitates the directed transfer of H_(2)O_(2),resulting in 2.4-fold and 5.0-fold elevation in catalytic activ-ity compared to free ECSs and separated ECSs,*** substrate channeling effect could be regulated by altering the mass ratio of GOx to ***,I-ECSs demonstrate excellent stabili-ties in harsh environments and multiple recycling.

关键词： Biocatalysis Immobilization Enzyme cascade system Substrate channeling effect Conjugated microporous polymers

Functional analysis of alternative splicing of the FLOWERING LOCUS T orthologous gene in Chrysanthemum morifolium

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2016年第1期3卷 51-58页

作者： Yachao Mao Jing Sun Peipei Cao Rong Zhang Qike Fu Sumei Chen Fadi Chen jiafu jiang College of Horticulture Nanjing Agricultural UniversityNanjing210095China

As the junction of floral development pathways,the FLOWERING LOCUS T(FT)protein called‘florigen’plays an important role in the process of plant flowering through signal *** isolated four transcripts encoding different isoforms of a FT orthologous gene CmFTL1,from Chrysanthemum morifolium cultivar‘Jimba’.Sequence alignments suggested that the four transcripts are related to the intron *** analysis showed that four alternative splicing(AS)forms of CmFTL1 varied depending on the developmental stage of the *** functional complement experiment using an Arabidopsis mutant ft-10 revealed that the archetypal and AS forms of CmFTL1 had the function of complementing late flower phenotype in different *** addition,transgenic confirmation at transcript level showed CmFTL1 and CmFTL1ast coexist in the same tissue type at the same developmental stage,indicating a post-transcriptional modification of CmFTL1 in ***,ectopic expression of different AS forms in chrysanthemum resulted in the development of multiple altered phenotypes,varying degrees of early *** found that an alternative splicing form(CmFTL1-astE134)without the exon 2 lacked the ability causing the earlier flower *** evidence in this study indicates that complex alternative processing of CmFTL1 transcripts in *** may be associated with flowering regulation and hold some potential for biotechnical engineering to create early-flowering phenotypes in ornamental cultivars.

关键词： LOCUS analysis cultivar

Temporal and Spatial Distribution of SARS-CoV-2 Aerosols in a Large-Scale Fangcang Shelter Hospital in Shanghai,China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering 2023年第9期28卷 222-233页

作者： jiafu jiang Zhe Yin Jing Li Leili Jia Rulin He Wenhui Yang Jihu Yang Hang Fan Sen Zhang Yunfei Wang Zengming Zhao Haoran Peng Lizhong Li Yi Yang Shi-Yong Fan Rong Xiang Jianshu Guo Jinjin Wang Juanning Wei Fengling Zhou Ding Liu Ping Zhao Yujun Cui Yunxi Liu Dongsheng Zhou Gang Dong Beijing Institute of Microbiology and Epidemiology Academy of Military Medical SciencesBeijing 100071China Chinese People’s Liberation Army Center for Disease Control and Prevention Beijing 100071China Department of Disease Prevention and Control the First Medical Center of Chinese PLA General HospitalBeijing 100853China The 902 Hospital of People’s Liberation Army Joint Logistics Support Force Bengbu 233015China General Hospital of Eastern Theater Command People’s Liberation ArmyNanjing 210002China Fourth Military Medical University Xijing HospitalXi’an 710032China General Hospital of Central Theater Command of Chinese People’s Liberation Army Wuhan 430070China Third Military Medical University Southwest HospitalChongqing 400038China Department of Microbiology Faculty of Naval MedicineNaval Medical UniversityShanghai 200433China Academy of Military Medical Sciences Institute of Pharmacology and Toxicology Beijing 100039China

The coronavirus disease 2019(COVID-19)pandemic caused by frequently mutating severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has had a worldwide ***,detailed data on the potential aerosol transmission of SARS-CoV-2 in real-world and controlled laboratory settings remain *** the COVID-19 pandemic in Shanghai,China in 2022,samples were collected in a Fangcang shelter hospital,a large-scale temporary hospital rapidly built by converting the existing National Exhibition and Convention Center(Shanghai)into a health care *** samples at different sites and intervals around patients and in public areas,surface samples,and pharyngeal swab samples from corresponding patients were *** were tested for SARS-CoV-2 using real-time quantitative polymerase chain reaction(RT-qPCR)assays,followed by sequencing if the cycle threshold(Ct)value was<*** positivity rate for SARS-CoV-2 in aerosol samples was high in contaminated zones(37.5%,104/277),especially around the bed(41.2%,68/165)and near ventilation inlets(45.2%,14/31).The prevalence of SARS-CoV-2 around the bed,public areas,and air inlets of exhaust vents fluctuated and was closely related to the positivity rate among patients at corresponding sampling *** surface samples of different personal protective equipment from medical staff had high positivity *** sequences of joined ORF1ab and spike genes obtained from sixty samples represented two main clusters of Omicron *** was consistency in virus sequences from the same patient and their environment,and the detected virus sequences matched those of virus strains in circulation during the collection periods,which indicated a high likelihood of cross-contamination in the Fangcang shelter *** summary,the results provide a quantitative and real landscape of the aerosol transmission of SARS-CoV-2 and a patient-centered view of contamination in large and enclosed spaces and offer a useful guid

关键词： Coronavirus disease 2019 Severe acute respiratory syndrome coronavirus 2 Aerosols Fangcang shelter hospital China

中小学生对数字教材的技术接受度及其影响因素研究

在线全文

学校读者我要写书评

暂无评论

现代远距离教育 2019年第4期 77-83页

作者：胡畔蒋家傅佛山科学技术学院

在教育云服务与智能终端技术快速发展的背景下,数字教材成为智慧教育的重要环节。如何增强数字教材的有用性与易用性,进而提升学生使用数字教材的行为意愿,是推动数字教材普及应用的关键因素。本研究以小学生、初中生及高中生为实验对象... 详细信息

在教育云服务与智能终端技术快速发展的背景下,数字教材成为智慧教育的重要环节。如何增强数字教材的有用性与易用性,进而提升学生使用数字教材的行为意愿,是推动数字教材普及应用的关键因素。本研究以小学生、初中生及高中生为实验对象,通过问卷调查研究学生对数字教材的技术接受程度。结果表明,学习者对数字教材的技术接受度取决于数字教材学习系统各项功能有用性与易用性的整体表现。同时,研究发现小学生与初中生对数字教材的使用意愿显著高于高中生,且学习者阅读电子书与使用平板电脑的经验会影响其对数字教材的技术接受度。依据研究结果,从数字教材的内容组织、交互设计、应用部署及推广策略等方面给出相应建议。

关键词：数字教材技术接受度有用性易用性行为意愿

基于改进简化PCNN模型的椒盐噪声滤波方法

在线全文

学校读者我要写书评

暂无评论

计算机工程与应用 2011年第18期47卷 191-194页

作者：蒋加伏申静朱德正长沙理工大学计算机与通信工程学院长沙410114

针对PCNN简化模型在图像滤波中存在的问题,首先运用反证法证明PCNN简化模型在图像椒盐噪声检测时对低亮度的椒噪声检测失效;然后采用分而治之的方法对PCNN简化模型进行了改进,得到一种改进的PCNN简化模型;最后利用改进的PCNN简化模型检... 详细信息

针对PCNN简化模型在图像滤波中存在的问题,首先运用反证法证明PCNN简化模型在图像椒盐噪声检测时对低亮度的椒噪声检测失效;然后采用分而治之的方法对PCNN简化模型进行了改进,得到一种改进的PCNN简化模型;最后利用改进的PCNN简化模型检测图像的污染程度,确定噪声的具体位置,自适应地确定中值滤波窗口的大小,实现图像的自适应中值滤波。实验结果表明,此方法提高了噪声检测的准确性、图像滤波的保真性,对不同密度的椒盐噪声都有较好的滤波性能。

关键词：自适应中值滤波脉冲耦合神经网络椒盐噪声

The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2018年第1期5卷 470-479页

作者： Yuying Qi Yanan Liu Zixin Zhang Jiaojiao Gao Zhiyong Guan Weimin Fang Sumei Chen Fadi Chen jiafu jiang College of Horticulture Key Laboratory of LandscapingMinistry of AgricultureNanjing Agricultural University210095NanjingChina

The enzyme RNAPII CTD phosphatase-like 1 is known as a transcriptional regulator of the plant response to various abiotic ***,the isolation of CmCPL1,a chrysanthemum(Chrysanthemum morifolium)gene encoding this enzyme is *** predicted 955 residue gene product includes the FCPH catalytic domain,two double-stranded RNA binding motifs,and a nuclear localization signal.A sub-cellular localization assay confirmed that CmCPL1 was expressed in the ***1 transcription was shown to be significantly inducible by heat *** over-expression and knockdown of CmCPL1,respectively,increased and diminished the tolerance of chrysanthemum to heat stress,which maybe dependent on the regulation of CmCPL1 and on the expression of downstream heat stress-responsive genes.

关键词： stress residue chrysanthemum

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

结合背景差分和闭合轮廓拟合的运动人体检测

计算机工程与应用 2015年第14期51卷 198-202页

作者：蒋加伏田莎莎长沙理工大学计算机与通信工程学院长沙410114

假定图像序列的背景图像已经获得,提出一种结合时空背景差和闭合轮廓拟合的运动人体目标检测算法。算法以较小的阈值获得当前帧的背景差分图像,并搜寻最大连通域为初始前景目标;构造加权高斯滤波器,提取初始前景目标边缘;拟合前景目标轮... 详细信息

假定图像序列的背景图像已经获得,提出一种结合时空背景差和闭合轮廓拟合的运动人体目标检测算法。算法以较小的阈值获得当前帧的背景差分图像,并搜寻最大连通域为初始前景目标;构造加权高斯滤波器,提取初始前景目标边缘;拟合前景目标轮廓,提出2 bit区域选择法提取不连续弧段的端点,根据类间最小距离准则构造闭合轮廓,结合帧间差分提取最终前景轮廓,标注运动目标位置。仿真实验表明,该算法能准确提取出运动目标,并标注目标位置,在背景环境发生变化时同样可以准确检测到运动目标,具有较好的鲁棒性。

关键词：背景差分闭合轮廓拟合运动人体检测视频分析

在线全文

学校读者我要写书评

暂无评论

菊花近缘野生种质资源研究进展及保护建议

科技导报 2023年第4期41卷 31-42页

作者：陈希蒋甲福陈发棣大理大学农学与生物科学学院大理671003 南京农业大学园艺学院南京210095

综述了菊花近缘野生种质资源的地理分布特征、多样性热点、研究现状、珍稀物种保护等,分析了中国特有的菊属、亚菊属以及其他珍稀菊科物种的生存及保护现状,提出了以菱叶菊、桌子山菊、密绒亚菊、太行菊、复芒菊、白菊木以及栌菊木为主... 详细信息

综述了菊花近缘野生种质资源的地理分布特征、多样性热点、研究现状、珍稀物种保护等,分析了中国特有的菊属、亚菊属以及其他珍稀菊科物种的生存及保护现状,提出了以菱叶菊、桌子山菊、密绒亚菊、太行菊、复芒菊、白菊木以及栌菊木为主的一批珍稀种质资源的研究进展和建议保护等级。从原生境调查与保护和濒危机制研究两方面阐述了珍稀物种保护的研究进展,并从前沿监测技术体系发掘以及多学科交叉分析的角度,提出了菊花近缘野生种质资源保护的研究方法和发展方向。

关键词：菊花种质资源特有物种

在线全文

学校读者我要写书评

暂无评论

基于雾计算的计算资源分配方案

计算机工程与应用 2019年第19期55卷 96-104页

作者：汤琳煜蒋加伏谷科长沙理工大学计算机与通信工程学院

雾计算可以为用户提供近距离的数据存储、计算和其他服务,因此雾计算中的任务调度和资源分配已经成为一个新的研究热点。考虑终端用户和雾设备通常处于一种相对开放的状态,扩展了雾计算的体系结构,提出一种开放式雾计算环境中基于稳定... 详细信息

雾计算可以为用户提供近距离的数据存储、计算和其他服务,因此雾计算中的任务调度和资源分配已经成为一个新的研究热点。考虑终端用户和雾设备通常处于一种相对开放的状态,扩展了雾计算的体系结构,提出一种开放式雾计算环境中基于稳定匹配的计算资源分配方案,利用雾网络中动态的计算资源协同为用户提供计算服务并收取计算收益,同时终端用户向雾服务器提交任务请求并支付一定的费用。基于稳定匹配的思想,利用子任务的优先级列表、子任务和计算服务设备的偏好列表解决子任务与计算服务设备的分配问题,保证任务的完成时间和计算服务设备的收益。通过实验对方案性能进行了分析,实验结果表明该方案的资源分配时间相对稳定,且在执行雾计算任务时延以及任务违规率上都优于SGA算法和ACOSA算法。

关键词：雾计算任务调度计算资源分配匹配策略