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BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum

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Molecular Horticulture 2023年第1期3卷 306-318页

作者： Yiwen Zhai Yuqing Zhu Qi Wang Guohui Wang Yao Yu Lijun Wang Tao Liu Shenhui Liu Qian Hu Sumei Chen Fadi Chen jiafu jiang National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization Key Laboratory of LandscapingMinistry of Agriculture and Rural AffairsKey Laboratory of Biology of Ornamental Plants in East ChinaNational Forestry and Grassland AdministrationZhongshan Biological Breeding LaboratoryCollege of HorticultureNanjing Agricultural UniversityNanjing 210095China

The quantitative control of FLOWERING LOCUS T(FT)activation is important for the floral transition in flowering ***,the flowering regulation mechanisms in the day-neutral,summer-flowering chrysanthemum plant remain *** this study,the chrysanthemum BBX7 homolog CmBBX7 was isolated and its flowering function was *** expression of CmBBX7 showed a diurnal rhythm and CmBBX7 exhibited higher expression levels than *** of CmBBX7 in transgenic chrysanthemum accelerated flowering,whereas lines transfected with a chimeric repressor(pSRDX-CmBBX7)exhibited delayed *** single hybridization,luciferase,electrophoretic mobility shift,and chromatin immunoprecipitation assays showed that CmBBX7 directly targets *** addition,we found that CmBBX7 and CmBBX8 interact to positively regulate the expression of CmFTL1 through binding to its ***,these results highlight CmBBX7 as a key cooperator in the BBX8–FT module to control chrysanthemum flowering.

关键词： BBX family Diurnal rhythm CmFTL1 Photoperiod Flowering transition

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EAST纵场电源均流技术

电工技术学报 2007年第9期22卷 118-123页

作者：江加福刘小宁许留伟廖燕川中国科学院等离子体物理研究所合肥230031

EAST纵场电源由两组双反星形整流电路直接并联而成,要求单组电源的环流和两组电源之间的环流必须满足规定。从纵场电源的数学模型着手,通过谐波分析得到产生环流的原因,对于单组电源的环流,通过改变电路的阻抗特性来抑制,而对于两组电... 详细信息

EAST纵场电源由两组双反星形整流电路直接并联而成,要求单组电源的环流和两组电源之间的环流必须满足规定。从纵场电源的数学模型着手,通过谐波分析得到产生环流的原因,对于单组电源的环流,通过改变电路的阻抗特性来抑制,而对于两组电源之间的环流,则通过控制的方法进行动态调节。仿真和实验结果证明,该方案能在保证纵场电源稳定的前提下,取得良好的均流效果。

关键词：双反星形并联环流谐波超导托卡马克

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创建学习型远程教育校外学习中心的几点思考

中国电化教育 2005年第8期 48-50页

作者：蒋家傅佛山科学技术学院信息与教育技术中心广东佛山528000

远程教育校外学习中心作为远程教育系统的学习活动基地、教育教学信息的双向交互通道和教学资源的集散地,其主要作用促进信息的流动和人际(师生之间、生生之间)互动。该文在解读学习中心内涵的基础上,结合一线学生的实际需求,运用创建... 详细信息

远程教育校外学习中心作为远程教育系统的学习活动基地、教育教学信息的双向交互通道和教学资源的集散地,其主要作用促进信息的流动和人际(师生之间、生生之间)互动。该文在解读学习中心内涵的基础上,结合一线学生的实际需求,运用创建学习型组织的原理,提出了利用博客(Blog)、BBS和ICQ等技术建立学习团队并开展团队学习,创建学习型学习中心的基本思路。

关键词：远程教育学习中心学习支持服务学习型组织

The Chrysanthemum nankingense Genome Provides Insights into the Evolution and Diversification of Chrysanthemum Flowers and Medicinal Traits

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Molecular Plant 2018年第12期11卷 1482-1491页

作者： Chi Song Yifei Liu Aiping Song Gangqiang Dong Hongbo Zhao Wei Sun Shyam Ramakrishnan Ying Wang Shuaibin Wang Tingzhao Li Yan Niu jiafu jiang Bin Dong Ye Xia Sumei Chen Zhigang Hu Fadi Chen Shilin Chen Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing 100700 China College of Pharmacy Hubei University of Chinese Medicine Wuhan 430065 China College of Horticulture Nanjing Agricultural University Key Laboratory of Landscape Agriculture Ministry of Agriculture Nanjing 210095 China Amway (China) Botanical R&D Center Wuxi 214115 China Department of Ornamental Horticulture School of Landscape Architecture Zhejiang Agriculture and Forestry University Hangzhou 311300 China Amway Corporation Buena Park CA 90622-5940 USA Wuhan Benagen Tech Solutions Company Limited Wuhan 430070 China Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden The Chinese Academy of Sciences Guangzhou 510650 China These authors contributed equally to this article

The Asteraceae (Compositae),a large plant family of approximately 24 000-35 000 species,accounts for^10% of all angiosperm species and contributes a lot to plant *** most representative members of the Asteraceae are the economically important chrysanthemums (Chrysanthemum L.)that diversified through reticulate *** is typically created by multiple evolutionary mechanisms such as wholegenome duplication 0NGD)or polyploidization and locally repetitive genome ***,the lack of genomic data from chrysanthemum species has prevented an in-depth analysis of the evolutionary mechanisms involved in their ***,we used Oxford Nanopore long-read technologyto sequence the diploid Chrysanthemum nankingense genome,which represents one of the progenitor genomes of domesticated *** analysis revealed that the evolution of the *** genome was driven by bursts of repetitive element expansion and WGD events including a recentWGD that distinguishes chrysanthemum from sunflower,which diverged from chrysanthemum approximately 38.8 million years *** of ornamental and medicinal traits in chrysanthemums are linked to the expansion of candidate gene families by duplication events including paralogous gene ***,our study of the assembled reference genome offers new knowledge and resources to dissect the history and pattern of evolution and diversification of chrysanthemum plants,and also to accelerate their breeding and improvement.

关键词： Chrysanthemum genome evolution whole-genome duplication nanopore sequencing flower evolution medicinal plant

基于栈式去噪自动编码器的边际Fisher分析算法

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计算机工程与应用 2017年第5期53卷 134-139页

作者：颜丹蒋加伏长沙理工大学计算机与通信工程学院长沙410114

特征学习是模式识别领域的关键问题。基于自动编码器的深度神经网络通过无监督预训练与有监督微调能够有效地提取数据中关键信息,形成特征。提出一种基于栈式去噪自编码器的边际Fisher分析算法,该算法将边际Fisher分析运用于有监督微调... 详细信息

特征学习是模式识别领域的关键问题。基于自动编码器的深度神经网络通过无监督预训练与有监督微调能够有效地提取数据中关键信息,形成特征。提出一种基于栈式去噪自编码器的边际Fisher分析算法,该算法将边际Fisher分析运用于有监督微调阶段,进一步提升算法的特征学习能力。实验结果表明,该算法与标准的栈式去噪自编码器和基于受限玻尔兹曼机的深度信念网相比,具有更好的识别效果。

关键词：特征学习深度学习人工神经网络栈式去噪自动编码器边际Fisher分析

Genomic and transcriptomic alterations following intergeneric hybridization and polyploidization in the Chrysanthemum nankingense×Tanacetum vulgare hybrid and allopolyploid (Asteraceae)

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Horticulture Research 2018年第1期5卷 839-853页

作者： Xiangyu Qi Haibin Wang Aiping Song jiafu jiang Sumei Chen Fadi Chen Key Laboratory of Landscape Agriculture College of HorticultureNanjing Agricultural UniversityMinistry of AgricultureNanjing210095China

Allopolyploid formation involves two major events:interspecific hybridization and polyploidization.A number of species in the Asteraceae family are polyploids because of frequent *** effects of hybridization on genomics and transcriptomics in Chrysanthemum nankingense×Tanacetum vulgare hybrids have been *** this study,we obtained allopolyploids by applying a colchicine treatment to a synthesized ***×*** ***-related amplified polymorphism(SRAP),methylation-sensitive amplification polymorphism(MSAP),and high-throughput RNA sequencing(RNA-Seq)technologies were used to investigate the genomic,epigenetic,and transcriptomic alterations in both the hybrid and *** genomic alterations in the hybrid and allopolyploids mainly involved the loss of parental fragments and the gain of novel *** DNA methylation level of the hybrid was reduced by hybridization but was restored somewhat after *** were more significant differences in gene expression between the hybrid/allopolyploid and the paternal parent than between the hybrid/allopolyploid and the maternal *** differentially expressed genes(DEGs)showed down-regulation in the hybrid/allopolyploid relative to the *** the non-additive genes,transgressive patterns appeared to be dominant,especially repression *** expression dominance was observed specifically for down-regulated *** methylase and methyltransferase genes showed differential expression between the hybrid and parents and between the allopolyploid and *** data indicate that hybridization may be a major factor affecting genomic and transcriptomic changes in newly formed *** formation of allopolyploids may not simply be the sum of hybridization and polyploidization changes but also may be influenced by the interaction between these processes.

关键词： alterations polyp additive

CmFTL2 is involved in the photoperiod- and sucrose-mediated control of flowering time in chrysanthemum

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Horticulture Research 2017年第1期4卷 374-379页

作者： Jing Sun Heng Wang Liping Ren Sumei Chen Fadi Chen jiafu jiang College of Horticulture Nanjing Agricultural UniversityNanjing210095China

The chrysanthemum genome harbors three FT-like genes:CmFTL1 and CmFTL3 are thought to act as regulators of floral induction under long-day(LD)and short-day(SD)conditions,respectively,whereas the function of CmFTL2 is currently *** objective of the present research was to explore the function of CmFTL2 in the determination of flowering time of the photo-insensitive chrysanthemum cultivar‘Floral Yuuka’,both in response to variation in the photoperiod and to the exogenous provision of *** leaves of‘Floral Yuuka’plants with 50 mM sucrose accelerated flowering and increased the level of CmFTL2 transcription in the leaf more strongly than either CmFTL1 or FTL3 under both long and SD *** profiling indicated that all three CmFTL genes were upregulated during floral *** relationship of the CmFTL2 sequence with that of other members of the PEBP family suggested that its product contributes to the florigen rather than to the anti-florigen *** heterologous expression of CmFTL2 in the Arabidopsis thaliana ft-10 mutant rescued the mutant phenotype,showing that CmFTL2 could compensate for the absence of *** results suggest that CmFTL2 acts as a regulator of floral transition and responds to both the photoperiod and sucrose.

关键词： photoperiod floral harbor

Current achievements and future prospects in the genetic breeding of chrysanthemum:a review

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Horticulture Research 2019年第1期6卷 378-396页

作者： jiangshuo Su jiafu jiang Fei Zhang Ye Liu Lian Ding Sumei Chen Fadi Chen State Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of LandscapingMinistry of Agriculture and Rural AffairsCollege of HorticultureNanjing Agricultural University210095 NanjingChina

Chrysanthemum(Chrysanthemum morifolium Ramat.)is a leading flower with applied value *** new chrysanthemum cultivars with novel characteristics such as new flower colors and shapes,plant architectures,flowering times,postharvest quality,and biotic and abiotic stress tolerance in a time-and cost-efficient manner is the ultimate goal for *** breeding strategies have been employed to improve the aforementioned traits,ranging from conventional techniques,including crossbreeding and mutation breeding,to a series of molecular breeding methods,including transgenic technology,genome editing,and marker-assisted selection(MAS).In addition,the recent extensive advances in high-throughput technologies,especially genomics,transcriptomics,proteomics,metabolomics,and microbiomics,which are collectively referred to as omics platforms,have led to the collection of substantial amounts of *** of these omics data with phenotypic information will enable the identification of genes/pathways responsible for important *** attempts have been made to use emerging molecular and omics methods with the aim of accelerating the breeding of ***,applying the findings of such studies to practical chrysanthemum breeding remains a considerable challenge,primarily due to the high heterozygosity and polyploidy of the *** review summarizes the recent achievements in conventional and modern molecular breeding methods and emerging omics technologies and discusses their future applications for improving the agronomic and horticultural characteristics of chrysanthemum.

关键词： breeding chrysanthemum cultivar

基于LBP和栈式自动编码器的人脸识别算法研究

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计算机工程与应用 2018年第2期54卷 163-167,245页

作者：易焱蒋加伏长沙理工大学计算机与通信工程学院长沙410114

LBP算法对光照敏感且能有效地提取图像的纹理结构特征。提出一种基于局部二值模式(Local Binary Pattern,LBP)和栈式自动编码器(Stacked Autoencoders,SAE)的人脸识别算法。用统一模式LBP算子提取分块后的人脸图像的直方图,按顺序连接... 详细信息

LBP算法对光照敏感且能有效地提取图像的纹理结构特征。提出一种基于局部二值模式(Local Binary Pattern,LBP)和栈式自动编码器(Stacked Autoencoders,SAE)的人脸识别算法。用统一模式LBP算子提取分块后的人脸图像的直方图,按顺序连接形成整幅图像的LBP特征,并将其作为栈式自动编码器的输入,完成进一步的特征提取,实现人脸图像的识别与分类。在Extended Yale B等数据库上的实验结果表明,该算法与传统的人脸识别算法和标准的栈式自动编码器相比,对光照变化有更强的鲁棒性,具有更好的识别效果。

关键词：人脸识别深度学习栈式自动编码器局部二值模式