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Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation

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Frontiers of Chemical Science and Engineering 2024年第4期18卷 41-48页

作者： Ziyi Chu Boyu Zhang Zhenhua Wu Jiaxu Zhang Yiran Cheng Xueying Wang jiafu Shi Zhongyi jiang School of Environmental Science&Engineering Tianjin UniversityTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering Tianjin 300072China State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

Organic matter-induced mineralization is a green and versatile method for synthesizing hybrid nanostructured materials,where the material properties are mainly influenced by the species of natural biomolecules,linear synthetic polymer,or small molecules,limiting their ***,we adopted dendrimer poly(amidoamine)(PAMAM)as the inducer to synthesize organosilica-PAMAM network(OSPN)capsules for mannose isomerase(MIase)encapsulation based on a hard-templating *** structure of OSPN capsules can be precisely regulated by adjusting the molecular weight and concentration of PAMAM,thereby demonstrating a substantial impact on the kinetic behavior of the MIase@OSPN *** MIase@OSPN system was used for catalytic production of mannose from Dfructose.A mannose yield of 22.24% was obtained,which is higher than that of MIase in organosilica network capsules and similar to that of the free *** overall catalytic efficiency(kcat/Km)of the MIase@OSPN system for the substrate D-fructose was up to 0.556 s^(-1)·mmol^(-1)·***,the MIase@OSPN system showed excellent stability and recyclability,maintaining more than 50% of the yield even after 12 cycles.

关键词： enzyme immobilization enzyme catalysis organosilica networks capsules sugar biosynthesis

Overexpression of CmSOS1 confers waterlogging tolerance in Chrysanthemum

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Journal of Integrative Plant Biology 2020年第8期62卷 1059-1064页

作者： Lijun Wang Jiaojiao Gao Zixin Zhang Weimiao Liu Peilei Cheng Wenting Mu Tong Su Sumei Chen Fadi Chen jiafu jiang State Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of LandscapingMinistry of AgricultureCollege of HorticultureNanjing Agricultural UniversityNanjing 210095China

Plants experiencing hypoxia(a shortage of oxygen)are unable to maintain aerobic respiration,which leads to an energy and carbohydrate *** pervasive and rapid accumulation of ethylene is an early and reliable response to hypoxic stress(Sasidharan and Voesenek 2015),producing an uptick in the accumulation of reactive oxygen species(ROS).This in turn triggers apoptosis in root cortex cells,eventually leading to the formation of lysigenous aerenchyma,a tissue from which ethylene is readily lost(Loreti et al.2016).In plants exposed to prolonged waterlogging,group VII ethylene response factors operate to activate the suite of genes required to establish anaerobic metabolism(Gibbs et al.2011);some of these genes are also modulated by ROS(Narsai et al.2011;Sasidharan and Voesenek 2015).The transcriptional activation of genes encoding group VII ethylene response factors is regulated by the hypoxia response attenuator 1(HRA1)protein(Pucciariello and Perata 2017).A link between the oxygen sensing and ROS signaling has been established involving the hypoxia‐responsive universal stress protein 1(HRU1),which interacts with the respiratory burst oxidase homolog D(RBOHD)(Gonzali et al.2015).Salinity also tends to induce ROS production(You and Chan 2015).

关键词： metabolism eventually maintain

Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni

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Horticulture Research 2020年第1期7卷 1330-1338页

作者： Wanwan Zhang Tianwei Gao Peiling Li Chang Tian Aiping Song jiafu jiang Zhiyong Guan Weimin Fang Fadi Chen Sumei Chen State Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of LandscapingMinistry of Agriculture and Rural AffairsCollege of HorticultureNanjing Agricultural UniversityNanjing 210095China College of Horticulture Xinyang Agricultural and Forestry UniversityXinyangHenanChina

Chrysanthemum is frequently attacked by aphids,which greatly hinders the growth and ornamental value of this plant *** transcription factors play an important role in the response to biotic stresses such as pathogen and insect ***,chrysanthemum CmWRKY53 was cloned,and its expression was induced by aphid *** verify the role of CmWRKY53 in resistance to aphids,CmWRKY53 transgenic chrysanthemum was ***53 was found to mediate the susceptibility of chrysanthemum to *** expression levels of secondary metabolite biosynthesis genes,such as peroxidase-and polyphenol oxidase-encoding genes,decreased in CmWRKY53-overexpressing(CmWRKY53-Oe)plants but dramatically increased in chimeric dominant repressor(CmWRKY53-SRDX)plants,suggesting that CmWRKY53 contributes to the susceptibility of chrysanthemum to aphids,possibly due to its role in the regulation of secondary metabolites.

关键词： WRKY53 resistance hinder

Gain-of-function mutations of AtNHX1 suppress sos1 salt sensitivity and improve salt tolerance in Arabidopsis

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Stress Biology 2021年第1期1卷 170-188页

作者： Isaiah Catalino M.Pabuayon jiafu jiang Hongjia Qian Jung-Sung Chung Huazhong Shi Department of Chemistry and Biochemistry Texas Tech UniversityLubbockTX 79424USA Current address:State Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of LandscapingMinistry of Agriculture and Rural AffairsKey Laboratory of Biology of Ornamental Plants in East ChinaNational Forestry and Grassland AdministrationCollege of HorticultureNanjing Agricultural UniversityNanjing 210095China Current address:Department of Agronomy Gyeongsang National UniversityJinju 52828South Korea

Soil salinity severely hampers agricultural *** salt stress,excess Na+accumulation causes cellular damage and plant growth retardation,and membrane Na+transporters play central roles in Na+uptake and exclusion to mitigate these adverse *** this study,we performed sos1 suppressor mutant(named sup)screening to uncover potential genetic interactors of SOS1 and additional salt tolerance ***-based cloning and sequencing identified a group of mutants harboring dominant gain-of-function mutations in the vacuolar Na+/H+antiporter gene *** gain-of-function variants of AtNHX1 showed enhanced transporter activities in yeast cells and increased salt tolerance in Arabidopsis wild type *** content measurements indicated that at the cellular level,these gain-of-function mutations resulted in increased cellular Na+accumulation likely due to enhanced vacuolar Na+***,the gain-of-function suppressor mutants showed reduced shoot Na+but increased root Na+accumulation under salt stress,indicating a role of AtNHX1 in limiting Na+translocation from root to *** also identified another group of sos1 suppressors with loss-of-function mutations in the Na+transporter gene ***-of-function mutations in AtHKT1 and gain-of-function mutations in AtNHX1 additively suppressed sos1 salt sensitivity,which indicates that the three transporters,SOS1,AtNHX1 and AtHKT1 function independently but coordinately in controlling Na+homeostasis and salt tolerance in *** findings provide valuable information about the target amino acids in NHX1 for gene editing to improve salt tolerance in crops.

关键词： sos1 suppressor AtHKT1 AtNHX1 Gain-of-function Salt tolerance

The zoonotic and natural foci characteristics of SARS-CoV-2

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Journal of Biosafety and Biosecurity 2021年第1期3卷 51-55页

作者： Zhenjun Li jiafu jiang Xiangdong Ruan Yigang Tong Shuai Xu Lichao Han jianguo Xu State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingPR China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingPR China Academy of Forest Inventory and Planning State Forestry and Grassland AdministrationBeijing 100714PR China Beijing Advanced Innovation Center for Soft Matter Science and Engineering College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijingPR China

The origin of SARS-CoV-2 is still an unresolved *** this study,we systematically reviewed the main research progress of wild animals carrying virus highly homologous to SARS-CoV-2 and analyzed the natural foci characteristics of *** complexity of SARS-CoV-2 origin in wild animals and the possibility of SARS-CoV-2 long-term existence in human populations are also *** joint investigation of corona virus carried by wildlife,as well as the ecology and patho-ecology of bats and other wildlife,are key measures to further clarify the characteristics of natural foci of SARS-CoV-2 and actively defend against future outbreaks of emerging zoonotic diseases.

关键词： SARS-CoV-2 COVID-19 Natural foci disease Zoonotic disease

Miura origami based reconfigurable polarization converter for multifunctional control of electromagnetic waves

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Photonics Research 2024年第3期12卷 581-586页

作者： ZHIBIAO ZHU YONGFENG LI ZHE QIN LIXIN jiang WENJIE WANG HONGYA CHEN jiafu WANG YONGQIANG PANG SHAOBO QU Shaanxi Key Laboratory of Artificially-Structured Functional Materials and Devices Air Force Engineering UniversityXi’an 710051China School of Electronic Science and Engineering Xi’an Jiaotong UniversityXi’an 710049China

Polarization is one of the basic characteristics of electromagnetic(EM)waves,and its flexible control is very important in many practical *** present,most of the multifunction polarization metasurfaces are electrically tunable based on PIN and varactor diodes,which are easy to operate and have strong real-time ***,there are still some problems in them,such as few degrees of freedom of planar structure control,complex circuit,bulky sample,and high *** view of these shortcomings,this paper proposes a Miura origami based reconfigurable polarization conversion metasurface for multifunctional control of EM *** interaction between the electric dipoles is changed by adjusting the folding angleθ,thereby tuning the operating frequency of the polarization conversion and the polarization state of the reflected *** mechanical control method brings more degrees of freedom to manipulate EM *** the processed sample is with lightweight and low *** verify the performance of the proposed origami polarization converter,a Miura origami structure loaded with metal split rings is designed and *** operating frequency of the structure can be tuned in different folding *** addition,by controlling the folding angleθ,linear-to-linear and linear-to-circular polarization converters can be realized at different folding *** proposed Miura origami polarization conversion metasurface provides a new idea for reconfigurable linear polarization conversion and multifunctional devices.

关键词： polarization reconfigurable tuning

Comparative Analysis of Leaf Trichomes, Epidermal Wax And Defense Enzymes Activities in Response to Puccinia horiana in Chrysanthemum and Ajania Species

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Horticultural Plant Journal 2020年第3期6卷 191-198页

作者： Yinjie Wang Jun Zeng Xiaolong Xia Yang Xu Jing Sun Jing Gu Hainan Sun Haini Lei Fadi Chen jiafu jiang Weiming Fang Sumei Chen College of Horticulture Nanjing Agricultural UniversityNanjing 210095China

White rust caused by Puccinia horiana is a destructive disease of chrysanthemum *** better understand the resistance mechanisms of composite species to this disease,the leaf cuticular traits,antioxidant and defensive enzymes activities of immune(Chrysanthemum makinoi ***)and highly susceptible(Ajania shiwogiku ***)species were *** density of two species was markedly different,negatively associated with plant resistance to *** wax load in *** *** was two times more than that in *** *** wax composition in immune one was abundant in esters and primary *** dismutase(SOD,EC 1.***.1.1),peroxidase(POD,EC 1.***.1.7),polyphenoloxidase(PPO,EC 1.***.18.1 or EC 1.***.3.2)and phenylalanine ammonia lyase(PAL,EC 4.***.1.5)activitieswere *** *** ***,the activity of SOD and POD increased rapidly after inoculation,whichmight be non-host induced reactive oxygen species(ROS)activated antioxidant enzymes,however SOD and POD remained a low and steady level in the highly susceptible one after *** increase in PPO activities after inoculation was observed in both species,however it remained higher in *** *** at the late period of *** in *** *** was induced after pathogen inoculation,but not in *** ***,suggesting that these two enzymes might contribute to the resistance to ***.

关键词： Chrysanthemum Puccinia horiana Resistance Leaf cuticular wax Enzyme activity

The Establishment and Application of Mobile Electronic Surveillance System for Infectious Diseases with the Help of China—Sierra Leone,2016-Present

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China CDC weekly 2021年第36期3卷 763-768页

作者： Guangyu Zhao Haorong Chen Yanfeng Yan jiafu jiang Lei Lin Baogui jiang Foday Sahr Stephen Sevalie Qiang Xu Jinjin Chen Henry Saidu Bangura Kandeh Bassie Kargbo Yajun Song Wei Liu Liqun Fang Yansong Sun State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina College of Life Science and Technology Beijing University of Chemical TechnologyBeijingChina College of Medicine and Allied Health Sciences University of Sierra LeoneFreetownSierra Leone Military Hospital WilberforceFreetownSierra Leone.

Introduction:Infectious disease surveillance has long been a challenge for low-income countries like Sierra *** approaches based on paper and Short Message Service(SMS)were subject to severe delays in obtaining,transmitting,and analyzing ***:During the China aid operation for fighting Ebola since the end of 2014,a mobile electronic surveillance system for infectious diseases(MESSID)was developed in collaboration with the Republic of Sierra Leone Armed Forces(RSLAF),which comprised an Android-based reporting system and a complementary web-based program designed by Active Server ***(***)with the main functions including surveillance,real-time reporting,and risk assessment of infectious ***:MESSID was successfully registered in June 2016 and had been used by all medical and health institutions in *** June 1,2016 to July 5,2021,34,419 cases were diagnosed with 47 infectious diseases of 5 categories,with a total of 42 clinical *** to traditional approaches based on paper and SMS,the MESSID showed flexibility,high efficiency,convenience,and ***:MESSID is an accessible tool for surveillance of infectious diseases in Sierra Leone and possibly in other African countries with similar needs,capable of improving timeliness of disease reporting,thus rendering a timely outbreak detection and response.

关键词： rendering Server Sierra

CmBES1 is a regulator of boundary formation in chrysanthemum ray florets

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Horticulture Research 2020年第1期7卷 1097-1108页

作者： Peilei Cheng Yanan Liu Yiman Yang Hong Chen Hua Cheng Qian Hu Zixin Zhang Jiaojiao Gao Jiaxin Zhang Lian Ding Weimin Fang Sumei Chen Fadi Chen jiafu jiang State Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of LandscapingMinistry of Agriculture and Rural AffairsCollege of HorticultureNanjing Agricultural University210095 NanjingChina

Chrysanthemum(Chrysanthemum morifolium)is an ideal model species for studying petal morphogenesis because of the diversity in the flower form across varieties;however,the molecular mechanisms underlying petal development are poorly ***,we show that the brassinosteroid transcription factor BRI1-EMS-SUPPRESSOR 1(CmBES1)in chrysanthemum(*** ***)is important for organ boundary formation because it represses organ boundary identity *** plants overexpressing CmBES1 displayed increased fusion of the outermost ray florets due to the loss of differentiation of the two dorsal petals,which developed simultaneously with the ventral ***-seq analysis of the overexpression lines revealed potential genes and pathways involved in petal development,such as CUP-SHAPED COTYLEDON(CUC2),CYCLOIDEA 4(CYC4),genes encoding MADS-box transcription factors and homeodomain-leucine zippers(HD-Zips)and auxin pathway-related *** study characterizes the role of CmBES1 in ray floret development by its modulation of flower development and boundary identity genes in chrysanthemum.

关键词： genes boundary expressing