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MicroRNAs Are Involved in Maize Immunity Against Fusarium verticillioides Ear Rot

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Genomics, Proteomics & Bioinformatics 2020年第3期18卷 241-255页

作者： Zijian Zhou Yan Cao Tao Li Xinghao Wang jiafa chen Hang He Wen Yao Jianyu Wu Huiyong Zhang State Key Laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China State Key Laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of Life SciencesHenan Agricultural UniversityZhengzhou 450002China School of Advanced Agricultural Sciences and School of Life Sciences State Key Laboratory of Protein and Plant Gene ResearchPeking UniversityBeijing 100871China

Fusarium ear rot(FER)caused by Fusarium verticillioides is one of the most common diseases affecting maize production *** results in severe yield losses and grain contamination with health-threatening *** most studies to date have focused on comprehensive analysis of gene regulation in maize during defense responses against *** infection,less is known about the role of micro RNAs(mi RNAs)in this *** used deep sequencing to compare small RNA libraries from the maize kernels of susceptible(N6)or resistant(BT-1)inbred lines from uninfected plants and upon *** *** found that pathogen exposure was accompanied by dynamic alterations in expression levels of multiple mi RNAs,including new members of previously annotated mi RNA families.A combination of transcriptomic,degradomic,and bioinformatics analyses revealed that ***-responsive mi RNAs and their potential target genes displayed opposite expression patterns in the susceptible and resistant *** category analysis uncovered preferential enrichment of the pathogen-responsive mi RNAs and their targets in the phenylpropanoid metabolic processes,plant–pathogen interactions,and plant phytohormone signal transduction ***,transgenic maize plants overexpressing mi R408 b exhibited reduced resistance to *** infection in a susceptible maize *** findings provide new insights into the regulatory roles of mi RNAs in maize immunity against FER and new resources for breeding disease resistance into maize.

关键词： Maize MicroRNA Fusarium ear rot Deep sequencing Disease resistance

Natural variation in maize gene ZmSBR1 confers seedling resistance to Fusarium verticillioides

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The Crop Journal 2024年第3期12卷 836-844页

作者： Yunxia Song Peipei Ma Jingyang Gao Chaopei Dong Zhao Wang Yifan Luan jiafa chen Doudou Sun Pei Jing Xuecai Zhang Weibin Song Zijian Zhou Jianyu Wu College of Agronomy Henan Agricultural UniversityZhengzhou 450002HenanChina College of Life Sciences Henan Agricultural UniversityZhengzhou 450002HenanChina Global Maize Program International Maize and Wheat Improvement Center(CIMMYT)Apdo 6-64106600 MexicoDFMexico National Maize Improvement Center Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China

Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease,but the genetics and mechanisms of resistance are not well *** this study,GWAS performed by MLM and 3VmrMLM identified 40 and 20 QTNs,associated with seedling blight *** methods identified 49 and 36 genes,*** verification of candidate gene ZmSBR1 identified by both methods showed that the resistance of a mutant line to seedling blight decreased by 0.37 grade points after inoculation with ***,compared with the *** length of the stem rot lesion caused by *** increased by 86%in mutant seedlings,and the relative length of the adult plant stalk rot increased by 35%in mutant plants compared to the wild type after inoculation with Fusarium *** analysis showed that expression of defense-related genes after inoculation was down-regulated in the mutant compared to the wild type,synthesis of secondary metabolites associated with resistance was reduced,and the immune response triggered by PAMP decreased,resulting in decreased resistance of mutant maize *** gene association analysis showed that most maize inbred lines carried the susceptible haplotype.A functional PCR marker was *** results demonstrated that ZmSBR1 conferred resistance to multiple Fusarium diseases at the seedling and adult growth stages and had important application value in breeding.

关键词： Seedling blight Stalk rot Multiple disease resistance

Energy-Efficient Bandwidth and Power Allocation in Relay-Assisted Multi-Layer Heterogeneous Networks with Energy Harvesting

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Journal of Shanghai Jiaotong university(Science) 2023年第6期28卷 822-830页

作者：高锦程赵宜升陈加法陈忠辉 Fujian Key Laboratory for Intelligent Processing and Wireless Transmission of Media Information College of Physics and Information EngineeringFuzhou UniversityFuzhou 350108China

Aiming at excessive users existing in a pico base station(PBS)in the multi-layer heterogeneous networks,the resource allocation problem of maximizing the energy efficiency of the networks is investigated in this *** deploying a relay node with energy harvesting function,the data of some users in the PBS can be transferred to an adjacent idle *** bandwidth and transmitting power of users and the relay node are both considered to formulate the resource allocation optimization *** objective is to maximize the energy eficiency of the whole heterogeneous networks under the constraints of the user's minimum data rate and energy *** suboptimal solution is obtained by using the particle swarm optimization(PSO)algorithm and quantum-behaved particle swarm optimization(QPSO)*** results show that the adopted methods have higher energy efficiency than the conventional fixed power and bandwidth *** addition,the time complexity of the adopted methods is relatively low.

关键词： energy harvesting heterogeneous networks energy efficiency

Enhancing maize's nitrogen-fixing potential through ZmSBT3, a gene suppressing mucilage secretion∞

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Journal of Integrative Plant Biology 2023年第12期65卷 2645-2659页

作者： Jingyang Gao Peijiang Feng Jingli Zhang Chaopei Dong Zhao Wang Mingxiang chen Zhongliang Yu Bowen Zhao Xin Hou Huijuan Wang Zhaokun Wu Razia Sultana Jemim Haidong Yu Doudou Sun Pei Jing jiafa chen Weibin Song Xuecai Zhang Zijian Zhou Jianyu Wu College of Agronomy State Key Laboratory of Wheat and Maize Crop ScienceHenan Agricultural UniversityZhengzhou 450002China College of Life Sciences Henan Agricultural UniversityZhengzhou 450002China State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China International Maize and Wheat Improvement Center(CIMMYT) El BatanTexcoco 56237Mexico

Maize(Zea mays)requires substantial amounts of nitrogen,posing a challenge for its *** work discovered that some ancient Mexican maize landraces harbored diazotrophic bacteria in mucilage secreted by their aerial *** see if this trait is retained in modern maize,we conducted a field study of aerial root mucilage(ARM)in 258 inbred *** observed that ARM secretion is common in modern maize,but the amount significantly varies,and only a few lines have retained the nitrogen‐fixing traits found in ancient *** mucilage of the high‐ARM inbred line HN5‐724 had high nitrogen‐fixing enzyme activity and abundant diazotrophic *** genome‐wide association study identified 17 candidate genes associated with ARM across three *** of one candidate gene,the subtilase family gene ZmSBT3,confirmed that it negatively regulates ARM ***,the ZmSBT3 knockout lines had increased biomass and total nitrogen accumulation under nitrogen‐free culture *** ARM was associated with three ZmSBT3 haplotypes that were gradually lost during maize domestication,being retained in only a few modern inbred lines such as HN5‐*** summary,our results identify ZmSBT3 as a potential tool for enhancing ARM,and thus nitrogen fixation,in maize.

关键词： maize aerial root mucilage nitrogen‐fixing ZmSBT3 GWAS gene cloning Diazotroph

Growth Effect of Biennial Seedlings of Half-sib Families of Cyclobalanopsis glauca

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Agricultural Biotechnology 2018年第5期7卷 159-161页

作者： Jiyou WU Yong chenG Minggao chen jiafa chen Zhe WU Dezhi LIAO Yao SHU Hunan Academy of Forestry Changsha 410004China Hunan Fulin Biological Science and Technology Co. Ltd.Changsha 410004China Forestry Institute of Changde City Changde 415104China

The seedling height and ground diameter of 42 half-sib families of biennial Cyclobalanopsis glauca were investigated and compared. The results showed that there were extremely significant differences between different families in the terms of seedling height and ground diameter. According to the seedling height and ground diameter, families 39, 27, 28 and 19 of C. glauca were superior to other families at the seedling stage.

关键词： Cyclobalanopsis glauca Half-sib families Seedling height Ground diameter

TWO BASIC TYPES OF CRITERIA FOR LEXICAL AMBIGUITY

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铜仁师专学报 1999年第1期1卷 20-26页

作者：陈加法田兴斌贵州大学 550025 铜仁师专英语系

The purpose of this paper is to try to clarify the notion of Iexical ambiguity, and discuss possible diagnostic criteria.

关键词：模糊词判别标准直接标准间接标准语言

Heritability Analysis of Growth Traits and Selection of Semi-sib Families of Pinus elliottii

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Agricultural Biotechnology 2018年第6期7卷 160-162页

作者： Jiyou WU Yong chenG jiafa chen Hongmei XIE Dezhi LIAO Huajun LI Zhiheng WEI Zhe WU Hunan Academy of Forestry Changsha 410004China Yongzhou Forestry Science Institute Yongzhou 425000China Hunan Fulin Biological Science and Technology Co. Ltd.Changsha 410004China

Growth traits of semi-sib families of Pinus elliottii were *** results showed that there were extremely significant differences between the *** families in terms of tree height,DBH and volume( P < 0.01),and the families could be selected *** family heritability and single plant heritability of growth traits of *** were *** family heritability of tree height,DBH and volume was 0.799,0.779 and 0.770 respectively,and their single plant heritability was 0.683,0.829 and 0.742 *** them,the family heritability of tree height was the highest,while the single plant heritability of DBH was the *** Duncan analysis of growth traits of the *** families revealed that families 12,7,15,4 and 6 were superior families and can be used as high-yield families.

关键词： Pinus elliottii Semi-sib families Growth traits Heritability

Monthly Changes in the Resin Yield of Semi-sib Families of Pinus elliottii

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Agricultural Biotechnology 2018年第6期7卷 184-187,191页

作者： Jiyou WU Yong chenG jiafa chen Dezhi LIAO Huajun LI Hongmei XIE Zhiheng WEI Zhe WU Hunan Academy of Forestry Changsha 410004China Yongzhou Forestry Science Institute Yongzhou 425000China Hunan Fulin Biological Science and Technology Co. Ltd.Changsha 410004China

Monthly changes in the resin yield of semi-sib families of Pinus elliottii during 2013-2015 were *** results showed that from April to October,the resin yield of the *** families was the lowest in April and the highest in *** were significant differences between various families in terms of resin *** change of resin yield was closely related to temperature,and the higher the temperature was,the higher the resin yield *** mainly produced resin from June to September.

关键词： Pinus elliottii Semi-sib families Resin yield Monthly changes

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鸡呼吸道疾病综合防治

畜牧兽医科学（电子版） 2022年第21期 172-174页

作者：陈家发江苏省宿迁市宿城区洋北兽医站江苏宿迁223800

社会经济的加速发展促使畜牧养殖业迎来了大规模发展,但同时传染疾病的风险随之增高,其中呼吸道疾病比较常见,对鸡群生产性能、生命安全造成严重影响。该病主要是由于养殖管理不科学、病原菌侵袭所致,传播速度较快,需重视其综合防治措... 详细信息

社会经济的加速发展促使畜牧养殖业迎来了大规模发展,但同时传染疾病的风险随之增高,其中呼吸道疾病比较常见,对鸡群生产性能、生命安全造成严重影响。该病主要是由于养殖管理不科学、病原菌侵袭所致,传播速度较快,需重视其综合防治措施。通过免疫接种、卫生管理、饲养管理及药物治疗等措施,控制环境温湿度等养殖要点,有效提高鸡群的抗病能力,降低呼吸道疾病发生风险,减少不利影响,从整体上提高养殖场效益。

关键词：鸡呼吸道疾病综合防治