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内蒙古牛呼吸道合胞体病毒的分离鉴定及其全基因组分析

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中国预防兽医学报 2024年第5期46卷 476-483页

作者：黄金田李静丁玉林王文龙高娃王金玲内蒙古农业大学兽医学院/农业农村部动物疾病临床诊疗技术重点实验室内蒙古呼和浩特010010 内蒙古自治区科学技术厅内蒙古呼和浩特010000 河套学院医学系/内蒙古自治区蜱媒人畜共患传染病重点实验室内蒙古巴彦淖尔015000

为确定引起内蒙古地区3月龄~6月龄荷斯坦犊牛暴发呼吸系统疾病的病原,并分析其基因组序列,本研究对病死犊牛剖检并观察其各脏器的剖检病变,制备各组织病理切片,观察其组织病变。结果可见犊牛肺脏呈深红色有光泽的肌肉样实变,心脏肥大,... 详细信息

为确定引起内蒙古地区3月龄~6月龄荷斯坦犊牛暴发呼吸系统疾病的病原,并分析其基因组序列,本研究对病死犊牛剖检并观察其各脏器的剖检病变,制备各组织病理切片,观察其组织病变。结果可见犊牛肺脏呈深红色有光泽的肌肉样实变,心脏肥大,肝脏表面散在细小的结节状灰白色病灶,肾脏表面散在细小深红色出血点;组织病理学观察可见典型间质性肺炎,多数各级细支气管腔和肺泡腔内巨噬细胞和淋巴细胞大量增生和浸润,偶见合胞体细胞,在各级细支气管部分黏膜上皮细胞浆内有大小不等的圆形红染的病毒包涵体;轻度纤维化和小坏死灶的变质性肝炎,急性炎性脾肿,多发性小出血灶的慢性硬化性肾小球肾炎。进一步进行RT-PCR检测,结果显示,扩增获得约600 bp的目的条带,测序后与genBank中相关基因序列比对,结果显示目的基因与牛呼吸道合胞体病毒(BRSV)美国分离株USII/S1(KU159366)的同源性最高(98.42%),表明该地区某牧场犊牛感染了BRSV。将该病例肺组织研磨上清液接种牛肾细胞(MDBK)盲传3代出现典型的细胞病变,经RT-PCR检测确认为BRSV,进一步表明分离到1株BRSV,命名为IM BRSV-39。分段扩增IM BRSV-39株的全基因组序列,经测序后拼接获得长度为15130 bp的BRSV全基因组序列。采用MegAlign软件分析IM BRSV-39株的全基因组序列、g基因序列和F基因序列的同源性及突变位点;采用MEgA 11软件构建分离株全基因组进化树分析其遗传进化关系。结果显示,IM BRSV-39株的全基因组序列、g基因和F基因序列均与BRSVⅢ亚型分离株相应基因序列的同源性最高、且位于同一进化分支,证实IM BRSV-39株属于BRSVⅢ亚型;突变位点分析结果显示:与BRSVⅢ亚型参考株相比,IM BRSV-39株g基因序列存在7个突变位点,F基因序列存在12个突变位点;g蛋白氨基酸序列存在4个突变位点,其中L13H处在g蛋白的胞质结构域(aa1~aa37);Y^(127)H、I^(139)T、P^(177)L、A^(186)T处在g蛋白的胞外结构域(aa66~aa257);F蛋白氨基酸序列存在5个氨基酸突变位点,其中M^(4)T、M^(7)T、S^(14)C处在F蛋白氨基末端信号肽区(aa1~aa26)。本研究检测并分离到1株BSRV,揭示了该病毒基因组的遗传进化特征,为国内BRSV的深入研究奠定了基础。

关键词：病毒包涵体牛呼吸道合胞体病毒全基因组遗传进化

Effects of bark beetle pheromones on the attraction of Monochamus alternatus to pine volatiles

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Insect Science 2010年第6期17卷 553-556页

作者： jian-Ting Fan Daniel R. Miller Long-wa Zhang jiang-Hua Sun School of Forestry and Bio-technology Zhejiang Forestry University Lin'an Zhejiang Province China State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China Southern Research Station USDA Forest Service Athens Georgia USA School of Forestry and landscape Architecture Anhui Agriculture University Hefei China

We evaluated the attraction ofMonochamus alternatus Hope （Coleoptera： Cerambycidae）, Dryocoetes luteus Blandford and Orthotomicus erosus Wollaston （Coleoptera： Curculionidae： Scolytinae） to multiple-funnel traps baited with the pine volatiles, ethanol and （＋）-α-pinene and the bark beetle pheromones, ipsenol and ipsdienol. M. alternatus were attracted to traps baited with ethanol and （＋）-α-pinene but not those baited with ipsdienol and ipsenol. Ipsdienol and ipsenol decreased catches ofM. alternatus in traps baited with ethanol and （＋）-α-pinene. Traps baited with either binary combinations of ethanol and （＋）-α-pinene or ipsdienol and ipsenol were attractive to D. luteus and O. erosus. The addition of ipsenol and ipsdienol to traps baited with ethanol and （＋）-α-pinene synergized attraction of O. erosus but not D. luteus .

关键词： bark beetle pheromones Dryocoetes luteus Monochamus alternatus Orthotomicus erosus pine volatiles

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西藏昌都丁青县孜珠寺藏古籍考察记

古籍保护研究 2024年第2期 103-114页

作者：萨仁高娃王沛白张王建海国家图书馆古籍馆国家古籍保护中心办公室西藏图书馆

2023年5月15日,“古象雄文化遗产调查及抢救性保护研究项目”课题组在西藏丁青县孜珠山(外象雄中心)启动古籍普查及调研座谈活动。本项目由中国经济时报社与国家古籍保护中心合作开展,在“中华古籍保护计划”框架下,对西藏昌都孜珠寺所... 详细信息

2023年5月15日,“古象雄文化遗产调查及抢救性保护研究项目”课题组在西藏丁青县孜珠山(外象雄中心)启动古籍普查及调研座谈活动。本项目由中国经济时报社与国家古籍保护中心合作开展,在“中华古籍保护计划”框架下,对西藏昌都孜珠寺所藏藏文古籍开展调研,旨在深度挖掘古象雄文化遗产的时代价值,探索古籍的文物和文献价值,在古籍普查的基础上进一步做好原生性保护与再生性保护。本文作为“古象雄文化遗产调查及抢救性保护研究项目”成果之一,详尽记录了本次考察的项目背景、考察目的、考察情况,并对文献价值进行评估,提出下一步工作计划。

关键词：西藏古象雄孜珠寺苯教古籍

Comparative transcriptome analysis reveals molecular adaptations underlying distinct immunity and inverted resting posture in bats

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Integrative Zoology 2023年第3期18卷 493-505页

作者： Jinwei WU Libiao ZHANg Chao SHEN Simon Yung wa SIN Caoqi LEI Huabin ZHAO Engineering Research Center of Eco-environment in Three gorges Reservoir Region of Ministry of Education China Three Gorges UniversityYichangChina guangdong Key Laboratory of Animal Conservation and Resource Utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationInstitute of ZoologyGuangdong Academy of SciencesGuangzhouChina College of Life Sciences Wuhan UniversityWuhanChina School of Biological Sciences The University of Hong KongHong Kong SARChina

Understanding how natural selection shapes unique traits in mammals is a central topic in evolutionary *** mammalian order Chiroptera(bats)is attractive for biologists as well as the general public due to their speciﬁc traits of extraordinary immunity and inverted resting ***,genomic resources for bats that occupy key phylogenetic positions are not sufﬁcient,which hinders comprehensive investigation of the molecular mechanisms underpinning the origin of speciﬁc traits in ***,we sequenced the transcriptomes of 5 bats that are phylo-genetically divergent and occupy key positions in the phylogenetic tree of *** combination with the available genomes of 19 bats and 21 other mammals,we built a database consisting of 10918 one-to-one ortholog genes and reconstructed phylogenetic relationships of these *** found that genes related to immunity,bone remodeling,and cardiovascular system are targets of natural selection along the ancestral branch of *** analyses revealed that the T cell receptor signaling pathway involved in immune adaptation is speciﬁcally enriched in ***,molecular adaptations of bone remodeling,cardiovascular system,and balance sensing may help to explain the reverted resting posture in *** study provides valuable transcriptome resources,enabling us to tentatively identify genetic changes associated with bat-speciﬁc *** work is among theﬁrst to advance our understanding of the molecular underpinnings of inverted resting posture in bats,which could provide insight into healthcare applications such as hypertension in humans.

关键词： adaptation bats immunity resting posture transcriptome

Emerging roles of the neural adaptor FE65 in neurite outgrowth

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Neural Regeneration Research 2018年第12期13卷 2085-2086页

作者： Wen Li wai wa Ray Chan Jacky Chi Ki Ngo Kwok-Fai Lau Shenzhen Institute of Advanced Technology Chinese Academy of Sciences School of Life Sciences Chinese University of Hong Kong

The brain is the third largest organ in the human body and consists of over80 billion neurons（Herculano-Houzel,2009）.Neurons are interconnected by neurite to form a complex neural network that allows the communicati... 详细信息

关键词： FE Emerging roles of the neural adaptor FE65 in neurite outgrowth gEF

Electroconvulsive therapy:80 years old and still going strong

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World Journal of Psychiatry 2019年第1期9卷 1-6页

作者： gábor gazdag gabor S Ungvari First Department of Psychiatry and Psychiatric Rehabilitation Jahn Ferenc South Pest HospitalBudapest 1204Hungary Department of Psychiatry and Psychotherapy Semmelweis University Medical School1083 BudapestHungary University of Notre Dame FremantleWA 6009Australia Division of Psychiatry School of MedicineUniversity of Western AustraliaCrawleyWA 6009Australia

Electroconvulsive therapy(ECT),which is among the oldest and most controversial treatments in the field of psychiatry,has its 80th birthday this *** this brief historical overview,the discovery of the therapeutic effects of convulsive therapy by LaszlóMeduna,and the circumstances that motivated Ugo Cerletti and Lucio Bini to use electricity as a means of seizure induction are ***’s original theory about the antagonism between epilepsy and schizophrenia has been replaced by hypotheses on the mechanism of action of *** position of ECT in modern psychiatry is also discussed with special attention to its most important clinical indications,including catatonia,and preand postpartum affective and psychotic states that are responsive to ECT and in which ECT may even be *** effects and comparison of ECT with recently developed brain stimulation methods are also *** negative media portrayal of ECT and its earlier misuse may have contributed to its negative professional and public perceptions indicated repeatedly in attitude *** negative attitude has played an important role in the decreasing use of ECT in the developed world and a reduction in access to ECT,which constitutes a violation of psychiatric patients’right to an effective treatment.

关键词： Electroconvulsive therapy History Indications Utilization Attitudes

Theoretical Study on the Localizations and Conversions of Excess Dielectrons inside Double-Cage-Like Single Molecule

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Theoretical Study on the Localizations and Conversions of Ex...

Theoretical Study on the Localizations and Conversions of Excess Dielectrons inside Double-Cage-Like Single Molecule

中国化学会第十二届全国量子化学会议

作者： Yin-Feng wa.g jia Li jiangen Huang Ding-wa Zhang jiangxi Province Key Laboratory of Coordination Chemistry/School of Chemistry and Chemical Engineering Jinggangshan University

The perfluorinated cages with all the exo polarized Cδ+-Fδ-bonds have been proposed to be efficient containers of excess *** manipulate the localizations and conversions of excess electrons and spin states,we presented the theoretical investigations on the double-cage-like single molecular solvated dielectron systems,e2@C20F18（NH）2C20F18（e2@BB’）at the B3LYP/6-31g（d）+4s4p theory ***’s found that the lone pair of excess electrons is confined inside one cage（left or right）to form two different singlet electromers（1L and 1R）,while the two excess electrons are,respectively,trapped inside different cages to form triplet structure（3,ground state）.Interestingly,the application of small external electric field of 21（-21）×10-4 au results in single electron transfer of 3→1L（1R）and such transfer is also a spin state conversion of T→S.

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Theoretical Study on the Localizations and Conversions of Ex...

Syntheses,structures,and reactivity of ruthenium(Ⅱ) hydride complexes containing Klui's oxygen tripodal ligand

中国化学会2013年中西部地区无机化学化工学术研讨会

作者： Yin-Feng wa.g jia Li jiangen Huang Ding-wa Zhang jiangxi Province Key Laboratory of Coordination Chemistry Institute of Applied ChemistrySchool of Chemistry and Chemical EngineeringJinggangshan University

The perfiuorinated cages with all the exo polarized C-F bonds have been proposed to be efficient containers of excess *** manipulate the localizations and conversions of excess electrons and spin states,we presented the theoretical investigations on the double-cage-like single molecular solvated dielectron systems,e@CF(NH)CF(e@BB’) at the B3LYP/6-31g(d) + 4s4p theory ***’s found that the lone pair of excess electrons is confined inside one cage(left or

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Science China Chemistry 2010年第10期53卷 2139-2143页

作者： Ng Ho-Yuen LAI Chun-Sing WILLiaMS ian D. LEUNg wa-Hung Department of Chemistry The Hong Kong University of Science and Technology

Treatment of Ru(CO)(Cl)(H)(PPh3)3 with NaLOEt (LOEt-= [CpCo{P(O)(OEt)2}3]-) afforded the hydride complex (PPh3)(CO)-LOEtRu(H) (1), which has been characterized by X-ray crystallography. Similarly, the tricyclohexylphosphine analogue, (PCy3)(CO)LOEtRu(H) (2), was synthesized from Ru(CO)Cl(H)(PCy3)2 and NaLOEt. Treatment of complex 1 with R'SO2N3 afforded the (arylsulfonyl)amido complexes LOEtRu(CO)(PPh3)(NHSO2R) (R = 2,4,6-i-Pr3C6H2 (3), 4-t-BuC6H4 (4)). The crystal structure of complex 3 has been determined. The Ru-N distance and Ru-N-S angle in 3 are 2.076(3) and 126.14(16)°, respectively. Reactions of complex 1 with acids have been studied.

关键词： ruthenium oxygen tripodal ligand hydride complex (arylsulfonyl)amido complex