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An efficient Plant Regeneration System of Hydrangea bretschneideri Dipp via Stem Segments as explants

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Phyton-International Journal of experimental Botany 2021年第2期90卷 595-604页

作者： Si ran Wang jia Yan Bu er Ha Yu’e Bai Institute of Forest Tree genetic Breeding Forestry CollegeInner Mongolia Agricultural UniversityHohhot010000China

Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden *** engineering technology has been successfully used in many plant species,but it is limited in *** we established an efficient regeneration system by using stem segments as explants for the first *** our study,the plant growth regulators(PgRs)were evaluated at the different regeneration processes,including axillary shoots regeneration and root *** found that the optimal concentration for axillary buds’induction was 2.0 mgL^(-1)6-BA and 0.5 mgL^(-1)1 IAA,its highest induction rate was 70%.Moreover,the highest axillary shoots proliferation coefficient was 10.7 on the Murashige and Skoog(MS)medium with 2.0 mgL^(-1)6-benzyladenine(BA),0.2 mgL^(-1)indole-3-butyric acid(IBA),and 1.0 mgL^(-1)gibberellin A3(gA3).The highest frequency of root induction was 80.0±0.06%by culturing the elongated shoots in 1/2 MS medium containing 0.1 mgL^(-1)*** summary,our study will provide an effective technology for large-scale propagation and important pathway for promoting the popularization and application of Hydrangea bretschneideri Dipp.

关键词： Hydrangea bretschneideri Dipp stem segments regeneration

Production of translocation by crossing two different alien substitutions in wheat

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Chinese Science Bulletin 1999年第14期44卷 1294-1297页

作者： Yiwen Li Shunxue Tang Tiehan Zhao Hui lia.g Hongjie Li Shuang’e jia Zhensheng Li Xu jia Acad Sinica Inst Genet State Key Lab Plant Cell & Chromosome Eng Beijing 100101 Peoples R China

The translocation frequency up to 3.7% has been obtained by crossing a Triticum-Thinopyrum substitution with a Triticum-Hayna/dia substitution. The pattern of translocations includes not only wheat-alien Robertson translocations but also inserting translocations involving Thinopyrum chromatin and Haynaldia chromatin. The result indicates that it may be a new method of producing translocations to make two different wheat-alien substitution cross with each other.

关键词： substitution hybridization translocation genomic in situ hybridization.

Synergistic Disinfection effects of Dual-wavelength Ultraviolet and Its Impact on Photoreactivation and Dark Repair

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Huanjing Kexue/environmental Science 2025年第3期46卷 1443-1449页

作者： Zhou, Tian-Hong lia.g, Yuan-Fu Sun, Zhe Li, Meng-Kai Wang, jia-Le Li, Wen-Tao Ben, Wei-Wei Zhang, guo-Zhen Qiang, Zhi-Min School of environment and Municipal engineering Lanzhou Jiaotong University Lanzhou730070 China State Key Laboratory of environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing100085 China

Ultraviolet-C（UV-C）irradiation can effectively deactivate pathogenic microorganisms. The far UV-C（200-230 nm）and conventional UV-C（250-280 nm）can damage the proteins and nucleic acids of pathogenic microorganisms，respectively. The combination of far and conventional UV-C has the potential of synergistic inactivation. However，relevant studies remain limited owing to the lack of appropriate experimental setups. Therefore，this study established a mini-fluidic photoreaction system equipped with a KrCl excimer lamp and a low-pressure mercury lamp. This system could independently/simultaneously deliver stable 222 nm（far UV-C）and 254 nm（conventional UV-C）irradiations. Subsequently，the system was used to investigate the synergistic effect of dual-wavelength UV-C（the combination of 222 nm and 254 nm UV-C）on escherichia coli（e. coli）inactivation and the subsequent impact on photo- and dark-reactivation. The results indicated that dual-wavelength UV-C had a significant synergistic effect on e. coli inactivation，with a synergistic coefficient up to 2.2. Additionally，comparing to 254 nm UV irradiation，the photo-reactivation of e. coli after dual-wavelength UV-C irradiation was weakened，with the maximum lg reactivation percentage reducing from 50.8% to 36.1%. Furthermore，e. coli after dual-wavelength UV-C irradiation exhibited dark decay，in which the inactivation efficiency was further enhanced during dark treatment. Therefore，these results suggest that dual-wavelength UV-C could inhibit the light reactivation and dark repair of e. coli，ensuring the water biosafety，as well as providing reference for the development of effective and safe water disinfection technologies. © 2025 Science Press. All rights reserved.

关键词： escherichia coli

Animal models of hepatitis e infection: Advances and challenges

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Hepatobiliary & Pancreatic Diseases International 2024年第2期23卷 171-180页

作者： Ze Xiang Xiang-Lin He Chuan-Wu Zhu jia-jia Yang Lan Huang Chun jiang jian Wu Chinese Consortium for the Study of Hepatitis e(CCSHe) Zhejiang University School of Medicine Hangzhou 310030China Department of Infectious Diseases The Fifth People’s Hospital of SuzhouSuzhou 215007China Department of Infection Management The Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215008China Department of Clinical Laboratory The Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215008China 不详

Hepatitis e virus(HeV)is one of the leading causes of acute viral hepatitis *** most of HeV infections are asymptomatic,some patients will develop the symptoms,especially pregnant women,the elderly,and patients with preexisting liver diseases,who often experience anorexia,nausea,vom-iting,malaise,abdominal pain,and *** infection may become chronic in immunosuppressed *** addition,HeV infection can also cause several extrahepatic *** exists in a wide range of hosts in nature and can be transmitted across ***,animals susceptible to HeV can be used as *** establishment of animal models is of great significance for studying HeV transmission,clinical symptoms,extrahepatic manifestations,and therapeutic strategies,which will help us understand the pathogenesis,prevention,and treatment of hepatitis *** review summarized the animal models of HeV,including pigs,monkeys,rabbits,mice,rats,and other *** each animal species,we provided a concise summary of the HeV genotypes that they can be infected with,the cross-species transmission pathways,as well as their role in studying extrahepatic manifestations,prevention,and treatment of HeV *** advantages and disadvantages of these animal models were also *** review offers new perspectives to enhance the current understanding of the research landscape surrounding HeV animal models.

关键词： Hepatitis e virus Animal models Pathogenesis Prevention Treatment

Venous thrombosis and prothrombotic factors in inflammatory bowel disease

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World Journal of gastroenterology 2014年第17期20卷 4857-4872页

作者： Fernando Magro Jo?o-Bruno Soares Dália Fernandes gastroenterology Department of Centro Hospitalar S?o Jo?o 4200-319 PortoPortugal Institute of Pharmacology and Therapeutics Faculty of MedicineUniversity of Porto4200-319 PortoPortugal IBMC Institute for Molecular and Cell BiologyUniversity of Porto4150-180 PortoPortugal gastroenterology Department of Hospital de Braga 4710-243 BragaPortugal gastroenterology Department of Centro Hospitalar da Cova da Beira 6200-251 Covilh?Portugal

Patients with inflammatory bowel disease (IBD) may have an increased risk of venous thrombosis (VTe). PubMed, ISI Web of Knowledge and Scopus were searched to identify studies investigating the risk of VTe and the prevalence of acquired and genetic VTe risk factors and prothrombotic abnormalities in IBD. Overall, IBD patients have a two- to fourfold increased risk of VTe compared with healthy controls, with an overall incidence rate of 1%-8%. The majority of studies did not show significant differences in the risk of VTe between Crohn’s disease and ulcerative colitis. Several acquired factors are responsible for the increased risk of VTe in IBD: inflammatory activity, hospitalisation, surgery, pregnancy, disease phenotype (e.g., fistulising disease, colonic involvement and extensive involvement) and drug therapy (mainly steroids). There is also convincing evidence from basic science and from clinical and epidemiological studies that IBD is associated with several prothrombotic abnormalities, including initiation of the coagulation system, downregulation of natural anticoagulant mechanisms, impairment of fibrinolysis, increased platelet count and reactivity and dysfunction of the endothelium. Classical genetic alterations are not generally found more often in IBD patients than in non-IBD patients, suggesting that genetics does not explain the greater risk of VTe in these patients. IBD VTe may have clinical specificities, namely an earlier first episode of VTe in life, high recurrence rate, decreased efficacy of some drugs in preventing further episodes and poor prognosis. Clinicians should be aware of these risks, and adequate prophylactic actions should be taken in patients who have disease activity, are hospitalised, are submitted to surgery or are undergoing treatment.

关键词： Acquired genetic Prothrombotic Venous thrombosis Risk of venous thrombosis Inflammatory bowel disease

Accurate surface normal representation to facilitate gradient coil optimization on curved surface

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Magnetic Resonance Letters 2023年第1期3卷 67-84,I0004页

作者： Hao Ren Hui Pan Feng jia Jan g.Korvink Zhenyu Liu Changchun Institute of Optics Fine Mechanics and PhysicsChinese Academy of SciencesChangchun130033China School of Optoelectronics University of Chinese Academy of SciencesBeijing101499China Department of Radiology Medical PhysicsMedical Center University of FreiburgFaculty of MedicineUniversity of FreiburgFreiburg79110Germany Institute of Microstructure Technology Karlsruhe Institute of Technology(KIT)Karlsruhe76049Germany

The design methods for gradient coils are mostly based on discrete extrinsic methods(e.g.,the BioteSavart integration calculation),for which the surface normal vector strongly influences any numerical calculation of the discretized *** studies are mostly based on regular or analytical surfaces,which allow normal vectors to be expressed *** certain applications,design methods for extending currentcarrying surfaces from developable or analytic geometries to arbitrary surfaces generated from a scanned point cloud are *** key task is to correctly express the discretized normal vectors to ensure geometrical accuracy of the designed ***,it has been proven that applying a Delaunay triangulation to approximate a smooth surface can result in the discrete elemental normal vectors converging to those of the original ***,this article uses Delaunay triangulation to expand upon previous design methods so that they encompass arbitrary piecewise continuous *** design methods,the stream function and the so-called solid isotropic material with penalization(SIMP)method,are used to design circumvolute and noncircumvolute gradient coils on general surfaces.

关键词： gradient coils Delaunay triangulation Stream function SIMP method

金水缓纤组分方Ⅱ抑制脂滴分解阻抑巨噬细胞M2极化改善肺纤维化

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药学学报 2025年第2期60卷 369-378页

作者：邵文博郑佳萍赵鹏张琴河南中医药大学河南省中医药防治呼吸病重点实验室呼吸疾病中医药防治省部共建协同创新中心河南中医药大学中医药科学院

本研究旨在探讨金水缓纤组分方Ⅱ改善肺纤维化的作用和机制。动物实验已获得河南中医药大学动物实验中心伦理委员会批准(批准号:IACUC-202306012)。采用博来霉素(bleomycin, BLM)构建小鼠肺纤维化模型,苏木精-伊红染色法(hematoxylin-eo... 详细信息

本研究旨在探讨金水缓纤组分方Ⅱ改善肺纤维化的作用和机制。动物实验已获得河南中医药大学动物实验中心伦理委员会批准(批准号:IACUC-202306012)。采用博来霉素(bleomycin, BLM)构建小鼠肺纤维化模型,苏木精-伊红染色法(hematoxylin-eosin staining, H&e)检测各组小鼠肺组织病理形态;马松染色(Masson staining)检测各组小鼠肺组织纤维化;免疫荧光(immunofluorescence, IF)及实时荧光定量PCR (real-time quantitative PCR,qPCR)检测各组小鼠肺组织I型胶原(collagen type I, COL I)、α-平滑肌肌动蛋白(α-smooth muscle actin, α-SMA)、纤连蛋白(fibronectin, FN)、白细胞介素(interleukin, IL)-1β、白细胞介素-6 (interleukin-6, IL-6)、肿瘤坏死因子α (tumor necrosis factor α, TNF-α)表达;流式细胞术(flow cytometry, FCM)检测各组小鼠肺泡灌洗液(BALF)中M1型、M2型巨噬细胞比例; IF和qPCR检测各组小鼠肺组织脂肪酶家族成员N (lipase family member N, LIPN)表达水平;Amplex Red游离脂肪酸检测试剂盒检测各组小鼠肺组织游离脂肪酸水平。采用IL-4诱导骨髓衍生巨噬细胞(bone marrow derived macrophages, BMDMs) M2极化, FCM检测各组细胞CD206+M2型巨噬细胞比例; IF检测各组细胞LIPN表达及脂滴分解。结果显示,在BLM诱导的肺纤维化小鼠中,金水缓纤组分方Ⅱ显著减轻BLM引起的肺泡炎症和胶原沉积,显著抑制小鼠肺组织成纤维细胞活化和下调BALF中M2型巨噬细胞比例,显著抑制小鼠肺组织LIPN表达和游离脂肪酸水平;在IL-4诱导的BMDMs M2极化模型,金水缓纤组分方Ⅱ显著抑制CD206+M2型巨噬细胞比例,下调LIPN表达和阻抑脂滴分解。上述研究结果表明,金水缓纤组分方Ⅱ可能通过抑制脂滴分解,下调脂肪酸水平,阻抑巨噬细胞M2极化,减轻BLM诱导的肺纤维化。

关键词：金水缓纤组分方Ⅱ 肺纤维化脂滴分解巨噬细胞M2极化脂肪酶家族成员N

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鄂尔多斯盆地榆林—神木地区千5段低压成因定量分析

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天然气地球科学 2025年第2期36卷 284-292页

作者：康锐杨勇闫小雄陈虹贾丽梁霞中国石油长庆油田分公司勘探开发研究院低渗透油气田勘探开发国家工程实验室中国地质科学院地质力学研究所自然资源部古地磁与古构造重建重点实验室自然资源部活动构造与地质安全重点实验室

鄂尔多斯盆地东部榆林—神木地区上二叠统石千峰组5段（千5段）具有明显的异常低地层压力特征，但目前对地层低压成因缺少系统的定量分析。鄂尔多斯盆地东部地区在中—新生代发生过多次构造抬升作用，造成该地区千5段发生明显的孔隙反... 详细信息

鄂尔多斯盆地东部榆林—神木地区上二叠统石千峰组5段（千5段）具有明显的异常低地层压力特征，但目前对地层低压成因缺少系统的定量分析。鄂尔多斯盆地东部地区在中—新生代发生过多次构造抬升作用，造成该地区千5段发生明显的孔隙反弹和温度降低。在早白垩世以来地层剥蚀量恢复的基础上，从孔隙反弹、温度降低两方面定量分析了异常低压的成因，结果显示孔隙反弹造成的地层压力降低值为12.44～24.02 MPa，降低比例为28.40%～53.25%，温度降低造成的地层压力降低值为9.72～17.17 MPa，降低比例为24.40%～43.08%，说明孔隙反弹和温度降低是榆林—神木地区千5段发育异常低压的主要原因，其中孔隙反弹是最重要的控制因素。上述因素与天然气沿微断裂和微裂缝的散失作用、轻烃扩散作用、地层水化学作用等共同造成了千5段现今异常低压分布格局。

关键词：异常低压构造抬升地层温度降低石千峰组鄂尔多斯盆地

Numerical study of effects of hydrogen addition on methane combustion behaviors

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Journal of Iron and Steel Research International 2023年第11期30卷 2173-2185页

作者： Dian-yu e Ling-yi Weng guang-wu Tang Jing Li guang-chao Wei jia-xin Cui jiangxi Provincial Key Laboratory for Simulation and Modelling of Particulate Systems Jiangxi University of Science and TechnologyNanchang330013JiangxiChina International Institute for Innovation Jiangxi University of Science and TechnologyNanchang330013JiangxiChina Shenzhen Smoore Technology Limited Shenzhen518102GuangdongChina ARC Research Hub for Computational Particle Technology Department of Chemical and Biological EngineeringMonash UniversityClaytonVIC3800Australia School of Transportation and Vehicle engineering Shandong University of TechnologyZibo255000ShandongChina

The methane combustion with hydrogen addition can effectively reduce carbon emissions in the iron and steel making industry,while the combustion mechanism is still poorly *** oxy-fuel combustion of methane with hydrogen addition in a 0.8 MW oxy-natural gas combustion experimental furnace was numerically studied to investigate six different combustion *** results show that the 28-step chemical reaction mechanism is the optimal recommendation for the simulation balancing the numerical accuracy and computational *** the hydrogen enrichment increases in fuel,the highest flame temperature ***,the chemical reaction accelerates with enlarging the peak of the highest flame temperature and intermediate OH *** the hydrogen enrichment reaches 75 vol.%,the flame front is the farthest,and the flame high-temperature zone occupies the largest proportion corresponding to the most vigorous chemical reactions in the same oxygen supply.

关键词： Oxy-fuel combustion Hydrogen Combustion mechanism Flame temperature Simulation Modeling