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A high throughput antiviral screening platform for alphaviruses based on Semliki Forest virus expressing eGFP reporter gene

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Virologica Sinica 2023年第4期38卷 585-594页

作者： Yu-jia Shi jia-qi li Hong-qing Zhang Cheng-lin Deng qin-Xuan Zhu Bo Zhang Xiao-Dan li Hunan Normal University School of MedicineChangsha410081China Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

Alphaviruses,which contain a variety of mosquito-borne pathogens,are important pathogens of emerging/reemerging infectious diseases and potential biological ***,no specific antiviral drugs are available for the treatment of alphaviruses *** most highly pathogenic alphaviruses are classified as risk group-3 agents,the requirement of biosafety level 3(BSL-3)facilities limits the live virus-based antiviral *** facilitate the antiviral development of alphaviruses,we developed a high throughput screening(HTS)platform based on a recombinant Semliki Forest virus(SFV)which can be manipulated in BSL-2 *** the reverse genetics approach,the recombinant SFV and SFV reporter virus expressing eGFP(SFV-eGFP)were successfully *** SFV-eGFP reporter virus exhibited robust eGFP expression and remained relatively stable after four passages in BHK-21 *** a broad-spectrum alphavirus inhibitor ribavirin,we demonstrated that the SFV-eGFP can be used as an effective tool for antiviral *** SFV-eGFP reporter virus-based HTS assay in a 96-well format was then established and optimized with a robust Z0 score.A section of reference compounds that inhibit highly pathogenic alphaviruses were used to validate that the SFV-eGFP reporter virus-based HTS assay enables rapid screening of potent broad-spectrum inhibitors of *** assay provides a safe and convenient platform for antiviral study of alphaviruses.

关键词： Alphavirus Semliki Forest virus Reporter virus High throughput screening Inhibitor

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Conceptual Model for Automatic Early Warning Information System of Infectious Diseases Based on Internet Reporting Surveillance System

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Biomedical and Environmental Sciences 2007年第3期20卷 208-211页

作者： jia-qi MA li-PING WANG XIAO-PENG qi XIAO-MING SHI GONG-HUAN YANG National Center for Public Health Surveillance and Information Services Chinese Center for Disease Control and Prevention Beijing 100050 China

Objective To establish a conceptual model of automatic early warning of infectious diseases based on internet reporting surveillance system, with a view to realizing an automated warning system on a dally basis and timely identifying potential outbreaks of infectious diseases. Methods The statistic conceptual model was established using historic surveillance data with movable percentile method. Results Based on the infectious disease surveillance information platform, the conceptual model for early warning was established. The parameter, threshold, and revised sensitivity and specificity of early warning value were changed to realize dynamic alert of infectious diseases on a dally basis. Conclusion The instructive conceptual model of dynamic alert can be used as a validating tool in institutions of infectious disease surveillance in different districts.

关键词： Infectious disease Automated warning Information system

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Ku80 gene G-1401T promoter polymorphism and risk of gastric cancer

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World Journal of Gastroenterology 2011年第16期17卷 2131-2136页

作者： jia-qi li Jie Chen Nan-Nan liu li Yang Ying Zeng Bin Wang Xue-Rong Wang Key Laboratory of Reproductive Medicine Department of Pharmacology Nanjing Medical University Nan-jing 210029 Jiangsu Province China Department of General Surgery First Affiliated Hospitalof Nanjing Medical University. Nanjing 210029 Jiangsu Province China

AIM:To evaluate the possible relationship between the Ku80 gene polymorphism and the risk of gastric cancer in ***:In this hospital-based case-control study of gastric cancer in jiangsu Province,China,we investigated the association of the Ku80 G-1401T (rs828907) polymorphism with gastric cancer risk.A total of 241 patients with gastric cancer and 273 age-and sexmatched control subjects were genotyped and analyzed by polymerase chain reaction-restriction fragment length ***:The frequencies of genotypes GG,GT and TT were 65.6%,22.8% and 11.6% in gastric cancer cases,respectively,and 75.8%,17.6% and 6.6% in controls,*** were significant differences between gastric cancer and control groups in the distribution of their genotypes (P=0.03) and allelic frequencies (P=0.002) in the Ku80 promoter G-1401T ***:The T allele of Ku80 G-1401T may be associated with the development of gastric cancer.

关键词： Ku80 Gastric cancer Polymorphism Promoter Carcinogenesis

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Identification of fangchinoline as a broad-spectrum enterovirus inhibitor through reporter virus based high-content screening

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Virologica Sinica 2024年第2期39卷 301-308页

作者： qiu-Yan Zhang jia-qi li qi li Yang Zhang Zhe-Rui Zhang Xiao-Dan li Hong-qing Zhang Cheng-lin Deng Feng-Xia Yang Yi Xu Bo Zhang The Joint Center of Translational Precision Medicine Department of Infections and DiseasesGuangzhou Institute of PediatricsGuangzhou Women and Children's Medical CenterGuangzhou510623China Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China University of Science and Technology of China Department of Life Sciences and MedicineHefei230026China Hunan Normal University School of MedicineChangsha410081China

Hand,foot,and mouth disease(HFMD)is a common pediatric illness mainly caused by enteroviruses,which are important human ***,there are no available antiviral agents for the therapy of enterovirus *** this study,an excellent high-content antiviral screening system utilizing the EV-A71-eGFP reporter virus was *** this screening system,we screened a drug library containing 1042 natural compounds to identify potential EV-A71 ***(FAN),a bis-benzylisoquinoline alkaloid,exhibits potential inhibitory effects against various enteroviruses that cause HFMD,such as EV-A71,CV-A10,CV-B3 and *** investigations revealed that FAN targets the early stage of the enterovirus life *** the selection of FAN-resistant EV-A71 viruses,we demonstrated that the VP1 protein could be a potential target of FAN,as two mutations in VP1(E145G and V258I)resulted in viral resistance to *** research suggests that FAN is an efficient inhibitor of EV-A71 and has the potential to be a broad-spectrum antiviral drug against human enteroviruses.

关键词： Hand,foot,and mouth disease(HFMD) Enterovirus EV-A71 High-content screening Fangchinoline(FAN)

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Recurrent acute liver failure associated with novel SCYL1 mutation:A case report

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World Journal of Clinical Cases 2019年第4期7卷 494-499页

作者： jia-qi li Jing-Yu Gong A S Knisely Mei-Hong Zhang jian-She Wang Department of Pediatrics Jinshan Hospital of Fudan University Institut für Pathologie Medizinische Universit?t Graz The Center for Pediatric liver Diseases Children’s Hospital of Fudan University

BACKGROUND Pediatric recurrent acute liver failure(RALF) with recovery between episodes is rare. Causes include autoimmune disease, which may flare and subside;intermittent exposure to toxins, as with ingestions; and metabolic disorders,among them the fever-associated crises ascribed to biallelic mutations in SCYL1,with RALF beginning in infancy. SCYL1 disease manifest with RALF, as known to date, includes central and peripheral neurologic and muscular morbidity(hepatocerebellar neuropathy syndrome). Primary ventilatory and skeletal diseases also have been noted in some *** SUMMARY We describe a Han Chinese boy in whom fever-associated RALF began at age 14 mo. Bilateral femoral head abnormalities and mild impairment of neurologic function were first noted aged 8 years 6 mo. liver biopsy after the third RALF episode(7 years) and during resolution of the fourth RALF episode(8 years 6 mo) found abnormal architecture and hepatic fibrosis, respectively. Whole-exome sequencing revealed homozygosity for the novel frameshift mutation c.92_93 insGGGCCCT, p.(H32 Gfs~*20) in SCYL1(parental heterozygosity confirmed).CONCLUSION Our findings expand the mutational and clinical spectrum of SCYL1 disease. In our patient a substantial neurologic component was lacking and skeletal disease was identified relatively late.

关键词： SCYL1 Recurrent acute liver failure Whole-exome sequencing Case report

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Annual advances of acupuncture research in 2022

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Traditional Medicine Research 2023年第7期8卷 24-34页

作者： jia-qi li Wen-Jing Huang Kai Zhang Mei-Dan Zhao Research Center of Experimental Acupuncture Science Tianjin University of Traditional Chinese MedicineTianjin 301617China Department of Acupuncture and Moxibustion Tianjin Gong An HospitalTianjin 300042China School of Medical Technology Tianjin University of Traditional Chinese MedicineTianjin 301617China

This article reviews the major advances in acupuncture research in 2022,including clinical reports,basic research,and *** terms of the type of literature,most of them are systematic reviews and clinical trials,while high-quality basic studies can also be *** innovative inventions and researches in this field are of increasing quality and in a wide range of fields,acupuncture is attracting more and more attention in the international *** particular,some acupuncture combined sensors such as H2-EC/SD co-therapy,precise positioning,and vivo monitoring of neurotransmitter has been used for oncological diseases and neuropathic *** has been shown to be beneficial in the treatment of pain,stroke,psychiatric disorders,cancer,COVID-19 and *** of the studies show that acupuncture can play a positive role in various diseases and provide evidence for clinical applications and mechanism research.

关键词： acupuncture pain stroke cancer COVID-19 review

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Multidimensional trapping by dual-focusing cylindrical vector beams with all-silicon metalens

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Photonics Research 2022年第5期10卷 1162-1169页

作者： jia-LU ZHU REN-CHAO JIN li-li TANG ZHENG-GAO DONG jia-qi li JIN WANG School of Physics Southeast UniversityNanjing 211189China

Dual-focusing effect with a cylindrical vector-light characteristic(i.e.,radial and azimuthal polarizations)is theoretically proposed and numerically demonstrated by spin-decoupled phase control with all-silicon *** to the polarization dependence,the pair of focusing cylindrical vector beams can be interchanged by orthogonally switching the polarization of incident *** demonstrate the unique contributions of focused radial and azimuthal vector beams to longitudinal and transverse optical forces on glass spheres,respectively,by calculations based on the Maxwell stress *** paper presents the use of all-silicon metalens with highly-compact vector beams,promising for applications such as multidimensional optical trapping.

关键词： cylindrical trapping radial

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Association betweenITGA2 C807T polymorphism and gastric cancer risk

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World Journal of Gastroenterology 2011年第23期17卷 2860-2866页

作者： Jie Chen Nan-Nan liu jia-qi li li Yang Ying Zeng Xiao-Mei Zhao lin-lin Xu Xuan Luo Bin Wang Xue-Rong Wang Department of Pharmacology Nanjing Medical University Nanjing210029 Jiangsu Province China Department of General Surgery First Affiliated Hospitalof Nanjing Medical University Nanjing 210029 Jiangsu Prov-ince China

AIM： To evaluate the impact of the ITGA2 gene polymorphism on gastric cancer risk. METHODS： A hospital-based case-control study was conducted, including 307 gastric cancer patients and 307 age- and gender-matched control subjects. The genotypes were identified by polymerase chain reaction-restriction fragment length polymorphism assay. RESULTS： The frequencies of the wild and variant genotypes in cases were significantly different from those of controls （P = 0.019）. Compared with individuals with the wild genotype CC, subjects with the variant genotypes （CT ＋ IT） had a significantly higher risk of gastric cancer （adjusted odds ratio = 1.57, 95% CI = 1.13-2.17, P = 0.007）. In stratified analyses, the elevated gastric cancer risk was especially evident in older individuals aged 〉 58 years, nonsmokers and rural subjects. Further analyses revealed that the variant genotypes were associated with poor tumor differentiation and adjacent organ invasion in the sub-analysis of gastric cancer patients. CONCLUSION： The ITGA2 gene C807T polymorphism may be associated with an increased risk of gastric cancer, differentiation and invasion of gastric cancer.

关键词： Gastric cancer Integrin ITGA2 Polymor-phism Genotype

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Broadband visible light absorber based on ultrathin semiconductor nanostructures

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Chinese Physics B 2020年第1期29卷 254-259页

作者： lin-Jin Huang jia-qi li Man-Yi Lu Yan-Quan Chen Hong-Ji Zhu Hai-Ying liu Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices School for Information and Optoelectronic Science and EngineeringSouth China Normal UniversityGuangzhou 510006China

It is desirable to have electromagnetic wave absorbers with ultrathin structural thickness and broader spectral absorption bandwidth with numerous applications in *** this paper,we theoretically propose and numerically demonstrate a novel ultrathin nanostructure absorber composed of semiconductor nanoring array and a uniform gold *** results show that the absorption covers the entire visible light region,achieving an average absorption rate more than 90%in a wavelength range from 300 nm to 740 nm and a nearly perfect absorption from 450 nm to 500 nm,and the polarization insensitivity performance is particularly *** absorption performance is mainly caused by the electrical resonance and magnetic resonance of semiconductor nanoring array as well as the field coupling *** designed broadband visible light absorber has wide application prospects in the fields of thermal photovoltaics and photodetectors.

关键词： ultrathin nanostructures electrical resonance magnetic resonance polarization insensitivity

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Taxonomic revision of the genus Mesechinus (Mammalia Erinaceidae) with description of a new species

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Zoological Research 2018年第5期39卷 335-347页

作者： Huai-Sen Ai Kai He Zhong-Zheng Chen jia-qi li Tao Wan Quan li Wen-Hui Nie Jin-Huan Wang Wei-Ting Su Xue-Long jiang Gaoligongshan National Nature Reserve Baoshan Yunnan 678000 China Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China The Kyoto University Museum Kyoto University Kyoto 606-8501 Japan College of life Sciences Anhui Normal University Wuhu Anhui 241000 China Nanjing Institute of Environmental Sciences Ministry of Environmental Protection Nanjing Jiangsu 210042 China

Hedgehogs in the genus Mesechinus （Family Erinaceidae）, which include two currently recognized species （ M. dauuricus and M. hughi）, are distributed from northeast Mongolia to the upper Amur Basin in Russia and adjacent areas in northeast and northern China. In recent years, a population of Mesechinus hedgehogs was discovered from Mt. Gaoligong, southwestern Yunnan, China, far from the known distribution range of the genus. Furthermore, these hedgehogs are the only known population to be distributed at elevations higher than 2 100 m and in sympatry with gymnures. To evaluate the taxonomic status of these hedgehogs, we examined specimens representing Mesechinus taxa in China and further conducted morphometric and karyotypic analyses. Our results supported the existence of four species in China. Specifically, we identified the hedgehogs from Mt. Gaoligong as a new species, Mesechinus wangi sp. nov., and recognized M. miodon, previously considered as a synonym of either M. dauuricus or M. hughi as a distinct species. Interestingly, we observed a supernumerary M4 on all specimens of Mesechinus wangi sp. nov., which is an extremely rare event in the evolution of mammalian dentition.

关键词： Mesechinus Taxonomy MorphometricsInhibitory cascade Karyotype New speciesSupernumerary molar

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