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Plasmid-based generation of neural cells from human fibroblasts using non-integrating episomal vectors

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Neural Regeneration Research 2019年第3期14卷 501-505页

作者： Shao-Bing Dai Ting Shen Ting-Ting Zheng jia-li pu Xin-Zhong Chen Department of Anesthesiology Women's Hospital School of Medicine Zhejiang University Department of Neurology Second Affiliated Hospital College of Medicine Zhejiang University

Differentiation of human fibroblasts into functional neurons depends on the introduction of viral-mediated transcription factors, which present risks of viral gene integration and tumorigenicity. In recent years, although some studies have been successful in directly inducing neurons through sustained expression of small molecule compounds, they have only been shown to be effective on mouse-derived cells. Thus, herein we delivered vectors containing Epstein-Barr virus-derived oriP/Epstein-Barr nuclear antigen 1 encoding the neuronal transcription factor, Ascl1, the neuron-specific microRNA, miR124, and a small hairpin directed against p53, into human fibroblasts. Cells were incubated in a neuron-inducing culture medium. Immunofluorescence staining was used to detect Tuj-1, microtubule-associated protein 2, neuron-specific nucleoprotein NeuN and nerve cell adhesion molecules in the induced cells. The proportion of Tuj1-positive cells was up to 36.7% after induction for 11 days. From day 21, these induced neurons showed neuron-specific expression patterns of microtubule-associated protein 2, NeuN and neural cell adhesion molecule. Our approach is a simple, plasmid-based process that enables direct reprogramming of human fibroblasts into neurons, and provides alternative avenues for disease modeling and neurodegenerative medicine.

关键词： nerve regeneration induced neurons plasmid-based human fibroblasts nucleofection Ascl1 miR124 p53 reprogramming neural regeneration

Effects of two silicone resin coatings on performance of FeSiAl magnetic powder cores

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Journal of Iron and Steel Research International 2024年第5期31卷 1279-1288页

作者： jia-qi liu Zheng-qu Zhu pu Wang Yi-fan li Jing Pang jia-quan Zhang School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing100083China Qingdao Yunlu Advanced Materials Technology Co. Ltd.Qingdao266232ShandongChina

Two silicon resins with excellent thermal stability,JH1123 and JH7102,are used as the insulated agents and binders for the gas-atomized FeSiAl powder,and corresponding magnetic powder cores(MPCs)are *** insulation capability and application prospects of the two silicon resins are evaluated by comparing the magnetic properties of the coated powder and *** scanning electron microscopy,energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy results show that uniform insulation layers are both formed on the powder ***1123 has stronger binding ability,and the JH1123-coated powder exhibits severe agglomeration,with d50(average particle size)approximately twice that of the JH7102-coated *** as-prepared MPCs exhibit outstanding soft magnetic ***,the permeability of FeSiAl@JH1123 is up to 74.0,which is 35.5%higher than that of FeSiAl@JH7102 because JH1123 can further improve the density of the *** for FeSiAl@JH7102,it has better direct current bias and lower core loss of 716.9 mW cm^(−3) at 20 mT and 1000 kHz due to its lower coercivity and greater anti-magnetic saturation ability.A comprehensive comparison shows that FeSiAl@JH1123 is suitable for medium and high frequency applications,while FeSiAl@JH7102 is more suitable for high frequency *** indicates that the use of JH1123 and JH7102 silicon resins for binding and insulated coating not only simplifies the preparation process of MPCs,but also enables the controlled production of MPCs for different applications.

关键词： FeSiAl Insulated coating Magnetic powder core Permeability Core loss

Propagation form of internal cracks induced by continuous casting soft reduction and control strategy for internal quality

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Journal of Iron and Steel Research International 2024年第3期31卷 622-633页

作者： pu Wang liang Chen Qun-wei Tang Wei-tao li Shao-xiang li jia-quan Zhang School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China Weifang Special Steel Group Co..Ltd. Weifang 262400ShandongChina School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

The propagation form of internal cracks induced by continuous casting soft reduction and the control strategy for enhancing the internal quality of 45 steel through industrial trials and a three-dimensional flow-heat transfer-solidification coupling model were *** results showed that the internal cracks induced by soft reduction exhibited a characteristic of being"coarse in the middle and fine at both ends",and displayed an elliptical arc distribution on the loose side of the strand cross *** cracks originated within the brittle temperature range and propagated inward to the liquid impenetrable temperature and outward to the zero ductility temperature or *** control strategy for enhancing the internal quality of the 45 steel strand through soft reduction is to adjust the casting speed or the reduction zone appropriately,ensuring that the central solid fraction of the reduction zone falls within the range of *** this point,a reasonable reduction amount is allocated to eliminate the center shrinkage cavities and center segregation,even if it results in minor reduction-induced cracks.

关键词： Continuous casting Soft reduction Crack propagation Internal quality Control strategy

Digital signal acquisition system for complex nuclear reaction experiments

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Nuclear Science and Techniques 2024年第1期35卷 124-133页

作者： Wei-liang pu Yan-lin Ye jian-ling Lou jia-Hao Chen School of Physics and State Key Laboratory of Nuclear Physics and Technology Peking UniversityBeijing 100871China Institute of Applied Physics and Computational Mathematics Beijing 100094China

A digital data-acquisition system based on XIA LLC products was used in a complex nuclear reaction experiment using radioactive ion beams.A flexible trigger system based on a field-programmable gate array(FPGA)parametrization was developed to adapt to different experimental sizes.A user-friendly interface was implemented,which allows converting script language expressions into FPGA internal control *** proposed digital system can be combined with a conventional analog data acquisition system to provide more *** performance of the combined system was veri-fied using experimental data.

关键词： Digital signal acquisition system Trigger Programmable logic Timestamp

Impact of cognition-related single nucleotide polymorphisms on brain imaging phenotype in Parkinson’s disease

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Neural Regeneration Research 2023年第5期18卷 1154-1160页

作者： Ting Shen jia-li pu Ya-Si jiang Yu-Mei Yue Ting-Ting He Bo-Yi Qu Shuai Zhao Ya-Ping Yan Hsin-Yi Lai Bao-Rong Zhang Department of Neurology of the Second Affiliated Hospital Zhejiang University School of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina Department of Neurology of the Second Affiliated Hospital Interdisciplinary Institute of Neuroscience and TechnologyKey Laboratory of Medical Neurobiology of Zhejiang ProvinceZhejiang University School of MedicineHangzhouZhejiang ProvinceChina Department of Neurology of Sir Run Run Shaw Hospital Zhejiang University School of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina College of Biomedical Engineering and Instrument Science Key Laboratory for Biomedical Engineering of Ministry of EducationZhejiang UniversityHangzhouZhejiang ProvinceChina

Multiple single nucleotide polymorphisms may contribute to cognitive decline in Parkinson’s disease. However, the mechanism by which these single nucleotide polymorphisms modify brain imaging phenotype remains unclear. The aim of this study was to investigate the potential effects of multiple single nucleotide polymorphisms on brain imaging phenotype in Parkinson’s disease. Forty-eight Parkinson’s disease patients and 39 matched healthy controls underwent genotyping and 7 T magnetic resonance imaging. A cognitive-weighted polygenic risk score model was designed, in which the effect sizes were determined individually for 36 single nucleotide polymorphisms. The correlations between polygenic risk score, neuroimaging features, and clinical data were analyzed. Furthermore, individual single nucleotide polymorphism analysis was performed to explore the main effects of genotypes and their interactive effects with Parkinson’s disease diagnosis. We found that, in Parkinson’s disease, the polygenic risk score was correlated with the neural activity of the hippocampus, parahippocampus, and fusiform gyrus, and with hippocampal-prefrontal and fusiform-temporal connectivity, as well as with gray matter alterations in the orbitofrontal cortex. In addition, we found that single nucleotide polymorphisms in α-synuclein(SNCA) were associated with white matter microstructural changes in the superior corona radiata, corpus callosum, and external capsule. A single nucleotide polymorphism in catechol-O-methyltransferase was associated with the neural activities of the lingual, fusiform, and occipital gyri, which are involved in visual cognitive dysfunction. Furthermore, DRD3 was associated with frontal and temporal lobe function and structure. In conclusion, imaging genetics is useful for providing a better understanding of the genetic pathways involved in the pathophysiologic processes underlying Parkinson’s disease. This study provides evidence of an association between genetic factors

关键词： cognition imaging genetics magnetic resonance imaging multi-modality Parkinson’s disease polygenic risk score single nucleotide polymorphism ultra-high field

Betaine addition to the diet alleviates intestinal injury in growing rabbits during the summer heat through the AAT/mTOR pathway

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1623-1637页

作者： Zimei li Junning pu Xiang Chen Yanbin Chen Xiaoyan Peng Jingyi Cai Gang jia Hua Zhao Gang Tian Animal Nutrition Institute Sichuan Agricultural UniversityChengduSichuan 611130China

Background The aim of this experiment was to investigate the effect of different levels of betaine(Bet)inclusion in the diet on the intestinal health of growing rabbits under summer heat.A total of 100 weaned Qixing meat rabbits aged 35 d with body weight of 748.61±38.59 g were randomly divided into 5 treatment groups:control group(basal diet)and Bet groups(basal diet+0.75,1.0,1.5 or 2.0 g/kg Bet).The average daily temperature in the rabbitry during the experiment was 30.48°C and the relative humidity was 69.44%.Results Dietary addition of Bet had no significant effect on growth performance and health status of growing rabbits(P>0.05),but it increased ileal secretory immunoglobulin A content compared to the control under summer heat(Pli height(VH)in the jejunum(P<0.05).Serum glucose levels were reduced,and the expression of SLC6A20 was up-regulated in jejunum and ileum of rabbits fed with 1.5 g/kg Bet(P<0.05).When added at 2.0 g/kg,Bet reduced serum HSP70 content,increased jejunal VH,and up-regulated duodenal SLC7A6,SLC38A2,mTOR and 4EBP-2 expression(P<0.05).Correlation analysis revealed that intestinal mTOR expression was significantly and positively correlated with SLC7A6,SLC38A2,SLC36A1 and IL-4 expression(P<0.05).Conclusions Dietary addition of Bet can up-regulate the expression of anti-inflammatory factors through the AAT/mTOR pathway,improve the intestinal immune function,alleviate intestinal damage in growing rabbits caused by summer heat,and improve intestinal health.

关键词： AAT/mTOR Betaine Growing rabbits Intestinal injury Summer heat

Insight into the Role of Isolated Gold Atoms-Ceria Conjunction in Catalyzing the Water-Gas Shift Reaction

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Chinese Journal of Chemistry 2024年第6期42卷 636-644页

作者： Xin-pu Fu Hui-Zhao Wei-Wei Wang Chun-jiang jia Key Laboratory for Colloid and Interface Chemistry Key Laboratory of Special Aggregated MaterialsSchool of Chemistry and Chemical EngineeringShandong UniversityJinanShandong250100 China

As the promising catalysts for the water-gas shift(WGS)reaction,the activity of Au-CeO_(2) composites is susceptible to the aggregation size and electronic state of Au *** reports were extensively focused on the discrepancy between nonmetallic Au and metallic Au nanoparticles,whereas the understanding of the authentic role of the isolated Au atoms was ***,we investigated the catalytic behavior and structural information over two types of Au/CeO_(2) catalysts,in which the predominant conjunctions were isolated Au1-CeO_(2) and Aun-CeO_(2),*** on comprehensive characterizations,particularly by in-situ Raman and in-situ DRIFTS,we found that the isolated Au atoms were responsible for enhancing the reducibility of the CeO_(2) *** CO adsorption ability on the isolated Au sites was significantly inferior to clustered Au atoms,especially at relatively high temperatures(>200°C).As a result,the boosted O vacancy on the isolated Au1-CeO_(2) conjunctions could improve the H2O activation ability for the Au-CeO_(2) catalysts and demonstrate a comparable activity to the clustered Aun-CeO_(2) *** work might deepen understanding of the catalytic function for the isolated Au1 site within Au/CeO_(2) systems while catalyzing the WGS reaction.

关键词： Isolated Au atoms Ceria-based catalysts Water-gas shift reaction Defect Adsorption

Real-time Energy Management Method for Electric-hydrogen Hybrid Energy Storage Microgrids Based on DP-MPC

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CSEE Journal of Power and Energy Systems 2024年第1期10卷 324-336页

作者： Qi li Xueli Zou Yuchen pu Weirong Chen School of Electrical Engineering Southwest Jiaotong UniversityChengdu 611756China

With the increasing presence of intermittent energy resources in microgrids,it is difficult to precisely predict the output of renewable resources and their load *** order to realize the economical operations of the system,an energy management method based on a model predictive control(MPC)and dynamic programming(DP)algorithm is *** method can reasonably distribute the energy of the battery,fuel cell,electrolyzer and external grid,and maximize the output of the distributed power supply while ensuring the power balance and cost optimization of the *** on an ultra-shortterm forecast,the output power of the photovoltaic array and the demand power of the system load are *** offline global optimization of traditional dynamic programming is replaced by the repeated rolling optimization in a limited period of time to obtain power values of each unit in the energy storage *** with the traditional DP,MILP-MPC and the logic based real-time management method,the proposed energy management method is proved to be feasible and effective.

关键词： Electric-hydrogen hybrid energy economic energy management improved dynamic programming microgrid model predictive control

Surface Elastic Effects on Electromechanical Responses of a Piezoelectric Semiconducting Nanobeam

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Acta Mechanica Solida Sinica 2024年第4期37卷 598-612页

作者： Aowen Bao Xiaobao li Yuxue pu Chunxiao Zhan School of Civil Engineering Hefei University of TechnologyHefei230009China

Piezoelectric semiconductors(PSCs)find extensive applications in modern smart electronic devices because of their dual properties of being piezoelectric and *** the increasing demand for miniaturization of these devices,the performance of their components needs to be carefully designed and optimized,especially when reduced to *** has been shown that surface elastic properties play a substantial role in the mechanical performance of nanoscale materials and *** on this understanding,the surface elastic effects,encompassing surface residual stress,surface membrane stiffness,and surface bending stiffness,are comprehensively taken into account to explore the electromechanical responses of a PSC ***,the flexoelectric effect on their responses is also systematically *** results of this work reveal that surface elastic properties predominantly influence mechanical performance,while the flexoelectric effect plays a more dominant role in electric-related quantities at the ***,the significance of surface bending rigidity,which was often underestimated in the earlier literature,is ***,owing to the flexoelectric effect,the linear distribution of electric potential and charge carriers along the length transforms into a nonlinear *** distributions of electric potential and charge carriers across the cross section are also evidently ***,the size-dependent responses are *** findings may provide valuable insights for optimizing electronic devices based on nanoscale PSCs.

关键词： Surface elastic effect Flexoelectricity Size effect Piezoelectric semiconductor