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Bis(7)-tacrine protects retinal ganglion cells against excitotoxicity via NMDA receptor inhibition

International Journal of Ophthalmology(English edition) 2011年第2期4卷 125-130页

作者： Zu-Hai Zhang, jia-hua fang Department of Ophthalmology the First Hospital of Jingzhou Yangtze University Jingzhou 434000 Hubei Province China Wuhan Institute of Neuroscience & Drug Research Jianghan University Wuhan 430056 Hubei Province China Department of Ophthalmology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China

AIM: To investigate whether bis (7)-tacrine, a multifunctional drug, inhibits N-methyl-D-aspartate (NMDA) -activated current in retinal ganglion cells (RGC) and provides neuroprotection against retinal cell damage. METHODS: Purified RGC cultures were obtained from retinas of 1-3 days old Sprague-Dawley (SD) rats, following a two-step immunopanning procedure. After 7 days of cultivation, the inhibition of NMDA-activated current by bis(7) -tacrine was measured by using patch-clamp recording techniques. In animal experiments, RGCs were damaged after intravitreal injection of NMDA (5 mu L, 40nmol) in adult rats. Bis (7)-tacrine(0.05, 0.1, 0.2mg/kg) or memantine(20mg/kg) was intraperitoneal administered to the rats fifteen minutes before intravitreally injection of NMDA. RGC damage was analyzed by histologic techniques, TUNEL and retrograde labeling techniques. RESULTS: Whole-cell patch-clamp recordings demonstrated that NMDA (30 mu mol/L) resulted in approximately -50 pA inward currents that were blocked by bis (7)-tacrine (1 mu mol/L). Histological examination and retrograde labeling analysis revealed that bis (7)-tacrine induced a significant neuroprotective effect against NMDA-induced cell damage 7 days after NMDA injection. TUNEL staining showed that pretreatment with bis(7)-tacrine was effective in ameliorating NMDA-induced apoptotic cell loss in the retinal ganglion cell layer 18 hours after injection. CONCLUSION: Bis (7)-tacrine possesses remarkable neuroprotective activities against retinal excitotoxicity through inhibition of NMDA receptors.

关键词： bis(7)-tacrine N-methyl-D-aspartate receptors excitotoxicity neuroprotection

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Influence of nitric oxide synthase and cyclooxygenase blockade on expression of cyclooxygenase and hemodynamics in rats with portal hypertension

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Hepatobiliary & Pancreatic Diseases International 2006年第4期5卷 564-569页

作者： Hui Cao, jia Xu, hua Liu, fang-Bin Meng, jiang-Feng Qiu and Zhi-Yong Wu Department of Surgery, Renji Hospital, College of Medicine, Shanghai jiaotong University, Shanghai 200127, China Department of Surgery Renji Hospital College of Medicine Shanghai Jiaotong University Shanghai 200127 China. xujia201800@***

BACKGROUND : The importance of nitric oxide (NO) in the pathogenesis of portal hypertension (PHT) has been extensively studied, but whether or not prostacyclin (PGI2) plays a role in formation and development of hyperdynamic circutatory state in PHT has not been verified. The present study was undertaken to investigate the possible interaction between prostacyclin (PGI2) and nitric oxide (NO) in the hyperdynamic circulatory state of rats with chronic portal hypertension (PHT), by measuring the hemodynamic changes and expression of cyclooxygenase (COX) mRNA in vessels and small intestine after administration of Nω- nitro-L-arginine (L-NNA) or indomethacin (INDO) either in the short-term (7 days) or long-term (15 days). METHODS: Ninety-seven male Sprague-Dawley rats were divided into three groups: intrahepatic portal hypertension (IHPH) induced by injection of CCl4, prehepatic portal hypertension (PHPH) induced by partial stenosis of the portal vein, and sham-operated controls (SO). Animals of each group received L-NNA or INDO either for 7 or 15 days, with saline as control. Splanchnic hemodynamics was measured by the radioactive microsphere technique. The concentration of NO in serum was determined as the nitrate; nitrite ratio (NO2-/NO3-, μmol/L) by a colorometric method, and that of PGI2 was measured by specific radioimmunoassay for its stable hydrolysis product 6-keto-PGF1α (pg/ml). The reverse transcription- polymerase chain reaction measured the levels of COX-1 mRNA in the superior mesenteric artery, thoracic aorta, and small intestine of these ***: Compared with SO rats, COX-1 mRNA expression and the concentrations of plasma 6-keto- PGF1α and serum NO2-/NO3- were enhanced in both IHPH and PHPH rats; splanchnic vascular resistance (SVR) decreased, but portal venous inflow (PVI) markedly increased (P<0.05). Seven or 15 days of L-NNA treatment reduced COX-1 mRNA expression in these vessels and the small intestine, concomitant with a significant decrease i

关键词： portal hypertension hyperhemodynamics prostacyclin cyclooxygenase nitric oxide

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Expression of cyclooxygenase in hyperdynamic portal hypertensive rats

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Hepatobiliary & Pancreatic Diseases International 2006年第2期5卷 252-256页

作者： Hui Cao, jia Xu, Rong hua, fang-Bin Meng, jiang-Feng Qiu and Zhi-Yong Wu Department of Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200127, China Department of Surgery Renji Hospital Shanghai Second Medical University Shanghai 200127 China.

BACKGROUND: By detecting hemodynamic changes, concentration of plasm prostacyclin ( PGI2) and expression of cyclooxygenase( COX) in vasculature and splanchnic tissues, we evaluated the relative contributions of PGI2 and COX mRNA expression to the hyperdynamic circulatory state in chronic portal hypertensive rats. METHODS: Fifty male Sprague-Dawley rats were divided into 3 groups: intrahepatic portal hypertension (IHPH, n = 18) by injection of CCl4, prehepatic portal hypertension (PHPH, n = 18) by partial stenosis of the portal vein, and sham-operated controls (SO, n =14). Splanchnic hemodynamics was measured by radioactive microsphere techniques and the concentration of PGI2 was detected by specific radioimmunoassay for its stable hydrolysis product 6-keto-PGF1α. Semi-quantitive reverse transcriptase-polyme-rase chain reaction (RT-PCR) was performed to measure the levels of COX-1 mRNA and COX-2 mRNA in the thoracic aorta, superior mesenteric artery( SMA),and small intestine of IHPH, PHPH and SO rats, respectively. RESULTS: Hyperdynamic circulatory state was characterized by increased splanchnic blood flow and decreased splanchnic vascular resistance in IHPH and PHPH rats. The concentration of plasma 6-keto-PGF1α ( pg/ml) in IHPH (1093.75 ± 142.15) and PHPH (897. 42 ± 53. 29) rats was significantly higher than that in SO rats (730.13 ± 98. 67) (P <0.05). The expression of COX-1 mRNA in the thoracic aorta, SMA and small intestine was enhanced, whereas COX-2 mRNA expression was not detected in either of these vessels or the small intestine. The plasma 6-keto-PGF1α concentration and the expression of COX-1 mRNA in these vessels and the small intestine were closely correlated with such hemodynamic parameters as portal venous inflow (PVI), splanchnic vascular resistance (SVR) and free portal venous pressure (FPP) (P<0.05). CONCLUSION: The expression of COX-1 mRNA and the levels of PGI2 were closely related to the hyperdynamic circulatory state of portal hypertensive rats.

关键词： hypertension, portal hyperhemodynamics prostacyclin cyclooxygenase

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调整房地产开发企业土地增值税税负的方法

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特区经济 2006年第6期 375-376页

作者：贾华芳襄樊学院经济与管理学系湖北襄樊441053

增值税为纳税人转让房地产的收入减除《土地增值税暂行条例实施细则》规定的扣除项目金额后的余额。可从确定适当房地产价格、成本换算对象、选择合适的利息扣除方式及运用税收优惠政策等方式调整税负。

关键词：土地增殖税房地产开发企业税负

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氢键链质子接受能力对质子转移反应影响的理论研究

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分子科学学报 2017年第4期33卷 282-290页

作者：易佳成方华南京林业大学理学院化学与材料科学系江苏南京210037

采用密度泛函B3LYP方法,6-311+g(d,p)基组,对甲酸与质子性溶剂分子形成的HCOOH-S_1-S_2(S_1和S_2分别为H_2O和NHF2)复合物在气相时发生的基态三质子转移反应过程进行了理论研究.4个甲酸复合物HCOOH-H_2O-H_2O,HCOOH-NHF2-NHF2,HCOOH-H_2O-NHF2及HCOOH-NHF2-H_2O中发生的三质子转移反应都是以异步协同质子迁移方式进行的.甲酸复合物中的氢键链组成和连接方式对基态三质子转移反应能垒有显著影响.HCOOH-S_1-S_2复合物中氢键链的质子接受能力可以表示为a×β1+b×β2(a+b=1).当a=0.45,b=0.55时,HCOOH-S_1-S_2中氢键链的质子接受能力和HCOOH-S_1-S_2复合物中的质子转移反应能垒成线性关系.氢键链的质子接收能力越强,反应能垒越低.

关键词：密度泛函氢键链质子接受能力基态质子转移

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毛泽东的监督文化思想及其当代价值

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湘潭大学学报（哲学社会科学版） 2017年第3期41卷 1-7页

作者：颜佳华方浩伟湘潭大学公共管理学院湖南湘潭411105

毛泽东监督文化思想是以马克思主义民主监督理论为基础,批判继承中国传统监督文化,以中国历史发展经验为参照,在中国革命与建设的实践中形成和发展起来的,是毛泽东关于监督文化的一系列基本观点所构成的思想理论体系,具有丰富的内涵。... 详细信息

毛泽东监督文化思想是以马克思主义民主监督理论为基础,批判继承中国传统监督文化,以中国历史发展经验为参照,在中国革命与建设的实践中形成和发展起来的,是毛泽东关于监督文化的一系列基本观点所构成的思想理论体系,具有丰富的内涵。当代监督文化是对毛泽东监督文化思想的继承与发展,毛泽东监督文化思想对推进当前的监督工作具有重要的指导意义。

关键词：毛泽东监督文化人民监督党内监督

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探索建立颅脑MRI图像、断层标本图像及三维重建图像的对比数据模型

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解剖学报 2016年第2期47卷 243-246页

作者：付廷刚张静黄忠浩贾方华齐鲁医药学院解剖学教研室山东淄博255213 齐鲁医药学院口腔修复教研室山东淄博255213 齐鲁医药学院影像学教研室山东淄博255213 齐鲁医药学院解剖学技术室山东淄博255213

目的利用计算机图像处理系统对颅脑主要重要结构进行三维成像,探索建立1套正常颅脑部MRI图像、断层标本及三维重建图像的数据对照计算机模型,为影像诊断及颅脑部手术定位提供形态依据。方法利用Microsoft Visual Studio将获得的MRI图像... 详细信息

目的利用计算机图像处理系统对颅脑主要重要结构进行三维成像,探索建立1套正常颅脑部MRI图像、断层标本及三维重建图像的数据对照计算机模型,为影像诊断及颅脑部手术定位提供形态依据。方法利用Microsoft Visual Studio将获得的MRI图像、断层标本图像及重建的三维图像建立数据模型。结果建立的数据模型提供了颅脑部正常解剖学影像学对比特点,构建了主要结构的三维立体模型图像,使抽象结构数字化、立体化、可视化,有助于对人脑的理解。结论本数据模型把颅脑结构的三维图像结合到标本MRI的对比图中,有利于初次接触颅脑断层的初学者。

关键词：颅脑磁共振成像三维图像数据模型人

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青光眼手术技术的进展

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国际眼科杂志 2013年第8期13卷 1572-1574页

作者：左国进方家华中国湖北省荆州市长江大学附属第一医院眼科 434000

手术是治疗青光眼的主要方法,为了减少术后并发症,提高手术效果,近年来出现了多种新型的手术方式。减少房水生成的手术已从传统的经巩膜睫状体光凝术改进到内窥镜下激光睫状体光凝术。就外引流手术而言,代表性的手术是传统的小梁切除术... 详细信息

手术是治疗青光眼的主要方法,为了减少术后并发症,提高手术效果,近年来出现了多种新型的手术方式。减少房水生成的手术已从传统的经巩膜睫状体光凝术改进到内窥镜下激光睫状体光凝术。就外引流手术而言,代表性的手术是传统的小梁切除术,近来出现了诸如Ex-Press微型引流钉植入术,Fugo刀经睫状体滤过术等新型手术。而内引流手术包括准分子激光小梁切开术、iStent植入术、内路小梁切开术、Schlemm管成形术以及金质微型引流器植入术。现将青光眼手术治疗进展做一综述。

关键词：青光眼手术治疗眼压

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