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Exosome-Transmitted miR-224-5p Promotes Colorectal Cancer Cell Proliferation via Targeting ULK2 in p53-Dependent Manner

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Biomedical and Environmental Sciences 2024年第1期37卷 71-84页

作者： YANG Le Mei ZHENG Qi LIU Xiao jia LI Xian Xian Veronica Lim CHEN Qi zhao Zhong Hua wang Shu Yang Department of Pathology School of Basic MedicineFudan UniversityShanghai 200032China Department of Pathology Dunedin School of MedicineUniversity of OtagoDunedin 9054New Zealand Department of Pathology Huashan HospitalFudan UniversityShanghai 200040China Shanghai Cancer Institute Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Teladoc Health Inc Shanghai 200335China Shanghai Medical College Fudan UniversityShanghai 200032China Shanghai Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention Shanghai 200032China

Objective To investigate the role and molecular mechanism of exosomal miR-224-5p in colorectal cancer(CRC).Methods The miR-224-5p expression in CRC patient tissues and cell-derived exosomes was measured by laser capture microdissection and qRT-PCR,***-luciferase reporter gene assay was used to determine the target gene of *** protein expressions of p53 and unc-51 like kinase 2(ULK2)in CRC cells were detected by western *** cytometry was used to detect cell cycle and *** proliferation was measured by CCK8 and EdU *** The miR-224-5p expression was upregulated in CRC tissues and increased progressively with the rise of CRC *** cells secreted extracellular miR-224-5p mainly in an exosome-dependent manner,and then miR-224-5p could be transferred to surrounding tumor cells to regulate cell proliferation in the form of autocrine or ***,ULK2 was characterized as a direct target of miR-224-5p and was downregulated in CRC ***,ULK2 inhibited CRC cell proliferation in a p53-dependent ***,exosome-derived miR-224-5p partially reversed the proliferation regulation of ULK2 on CRC *** Our findings demonstrate that exosome-transmitted miR-224-5p promotes p53-dependent cell proliferation by targeting ULK2 in CRC,which may offer promising targets for CRC prevention and therapy.

关键词： miR-224-5p Exosome ULK2 p53 Cell proliferation Colorectal cancer

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Understanding the mechanisms and potential pathways of soil carbon sequestration from the biogeochemistry perspective

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Science China Earth Sciences 2024年第11期67卷 3386-3396页

作者： Xiaojuan FENG Guohua DAI Ting LIU Juan jia Erxiong ZHU Chengzhu LIU Yunpeng zhao Ya wang Enze KANG Jun XIAO Wei LI State Key Laboratory of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences China National Botanical Garden College of Resources and Environment University of Chinese Academy of Sciences Department of Earth Sciences National Natural Science Foundation of China

Soil carbon sequestration is listed by the United Nations Framework Convention on Climate Change as one of the key ways to achieve long-term “carbon neutrality” in the context of global warming. Soil carbon sequestration is a complex biogeochemical process that involves plants, microbes, and rock minerals at its core. Yet, its regulation mechanisms and promotion pathways remain unclear. This paper reviews recent progress in the related domestic and international research and provides an overview of the key processes and mechanisms of soil carbon sequestration. The main pathways for enhancing soil carbon sequestration(including plant inputs, mineral protection, microbial transformation, and rock weathering) are summarized. The paper also discusses and synthesizes how advanced biogeochemical methods and technologies may be employed to explore soil carbon sequestration mechanisms and potentials. The overall aim of this review is to improve our understanding of soil carbon sequestration as a nature-based solution to combatting climate change from the biogeochemistry perspective, and to highlight the role of fundamental research in Earth Sciences in helping to achieve China's carbon neutrality goals.

关键词： Carbon neutrality Plant input Microbial carbon pump Mineral protection Rock weathering

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Lysophosphatidylcholine binds α-synuclein and prevents its pathological aggregation

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National Science Review 2024年第6期11卷 119-128页

作者： Chunyu zhao jia Tu Chuchu wang Wenbin Liu Jinge Gu Yandong Yin Shengnan Zhang Dan Li jiajie Diao Zheng-jiang Zhu Cong Liu Interdisciplinary Research Center on Biology and Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences University of Chinese Academy of Sciences Bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders Ministry of Education Shanghai Jiao Tong University Zhangjiang Institute for Advanced Study Shanghai Jiao Tong University Department of Cancer Biology University of Cincinnati College of Medicine

Accumulation of aggregated α-synuclein(α-syn) in Lewy bodies is the pathological hallmark of Parkinson's disease(PD). Genetic mutations in lipid metabolism are causative for a subset of patients with Parkinsonism. The role of α-syn's lipid interactions in its function and aggregation is recognized, yet the specific lipids involved and how lipid metabolism issues trigger α-syn aggregation and neurodegeneration remain unclear. Here, we found that α-syn shows a preference for binding to lysophospholipids(LPLs),particularly targeting lysophosphatidylcholine(LPC) without relying on electrostatic interactions. LPC is capable of maintaining α-syn in a compact conformation, significantly reducing its propensity to aggregate both in vitro and within cellular environments. Conversely, a reduction in the production of cellular LPLs is associated with an increase in α-syn accumulation. Our work underscores the critical role of LPLs in preserving the natural conformation of α-syn to inhibit improper aggregation, and establishes a potential connection between lipid metabolic dysfunction and α-syn aggregation in PD.

关键词： α-synuclein Parkinson's disease lysophosphatidylcholine, aggregation

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A Modeling Study Recurrent Tuberculosis Towards End TB Strategy—China,2025-2035

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China CDC weekly 2024年第35期6卷 885-890页

作者： Tao Li Xin Du Zhongwei jia Yanlin zhao Department of Epidemiology and Biostatistics School of Public HealthPeking UniversityBeijingChina Chinese Center for Disease Control and Prevention BeijingChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionBeijingChina Center for Intelligent Public Health Institute for Artificial IntelligencePeking UniversityBeijingChina Department of Global Health School of Public HealthPeking UniversityBeijingChina Center for Drug Abuse Control and Prevention National Institute of Health Data SciencePeking UniversityBeijingChina

Summary What is already known about this topic?The recurrence of tuberculosis(TB)following successful treatment presents a significant *** is added by this report?Achieving the global End TB Strategy milestones and targets with the current strategies in China is ***,interventions following recovery to prevent recurrence,in conjunction with preventive treatment for latent TB infection(LTBI),will aid in meeting these objectives.

关键词： Strategy tuberculosis treatment

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Slc43a2^(+)T cell metastasis from spleen to brain in RGNNV infected teleost

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Science China(Life Sciences) 2024年第4期67卷 733-744页

作者： Qing wang Yali Liu Minlin Zhang Min Yang jiantao Liang Xiaoling Zuo Shaowen wang Xianze jia Huihong zhao Han jiang Qiang Lin Qiwei Qin College of Marine Sciences South China Agricultural UniversityGuangzhou 510642China CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Nansha-South China Agricultural University Fishery Research Institute Guangzhou 511457China Joint University Laboratory of Guangdong Province Hong Kong and Marco Region on Marine Bioresource Conservation and ExploitationGuangzhou 510642China University of Chinese Academy of Sciences Beijing 100101China

The origin of T cells in the teleost's brain is *** viewing the central nervous system(CNS)as immune privileged has been widely accepted,previous studies suggest that T cells residing in the thymus but not in the spleen of the teleost play an essential role in communicating with the peripheral ***,we identified nine T cell subpopulations in the thymus and spleen of orange-spotted grouper(Epinephelus coioices)through single-cell RNA-sequencing *** viral CNS infection with red-spotted grouper nervous necrosis virus(RGNNV),the number of slc43a2^(+)T cells synchronously increased in the spleen and *** the infection tests in asplenic zebrafish(tlx1^▲zebrafish model),no increase in the number of slc43a2^(+)T cells was observed in the ***-cell transcriptomic analysis indicated that slc43a2^(+)T cells mature and functionally differentiate within the spleen and then migrate into the brain to trigger an immune *** study suggests a novel route for T cell migration from the spleen to the brain during viral infection in fish.

关键词： central nervous system slc43a2^(+)T cell RGNNV T cell migration teleost

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Upcycling of polyphenylene oxide into 2,6-dimethyl aniline via depolymerization and amination over a Pt/CeOcatalyst

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Science China Chemistry 2024年

作者： Shengchao jia Wenke zhao Xiaohui Liu Yong Guo Yanqin wang State Key Laboratory of Green Chemical Engineering and Industrial Catalysis Research Institute of Industrial Catalysis School of Chemistry and Molecular Engineering East China University of Science and Technology Shanghai Key Laboratory of Functional Materials Chemistry School of Chemistry and Molecular Engineering East China University of Science and Technology

Upcycling of plastic waste into N-containing compound is a novel and attractive strategy for its disposal and will opens up a promising direction for the production of value-added ***,an efficient strategy for the depolymerization-amination of polyphenylene oxide （PPO） into 2,6-dimethyl aniline is developed over a simple Pt/*** C–O linkages of PPO can be selectively broken,producing 2,6-dimethyl phenol and then hydrogenated and aminated to 2,6-dimethyl cyclohexylamine over the Pt/***,2,6-dimethyl aniline was produced with high selectivity through the dehydrogenation of 2,6-dimethyl cyclohexylamine in the same *** can be used as the hydrogen-acceptor in the dehydrogenation step and enhance the selectivity of target *** N-containing compound,2,6-dimethyl aniline,is obtained and separated after HCl ***,the Pt/CeO2catalyst exhibit remarkable stability and can sustainably catalyze the synthesis of 2,6-dimethyl aniline in several cycles.

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Production and characterization of monoclonal antibodies against grass carp CD4-1 and CD4-2

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Aquaculture and Fisheries 2024年第2期9卷 154-163页

作者： Bangjie wang Yuting Qin zhaosheng Sun Xiaozhen Zhu zhao jia Kangyong Chen Danjie Liu Junya wang Jun Zou Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China International Research Center for Marine Biosciences at Shanghai Ocean University Ministry of Science and TechnologyShanghai201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China

CD4 T helper cells are an important group of cells in the immune system of vertebrates and express CD4 receptor on the cell *** mammals,the CD4 receptor is encoded by a single copy gene,whilst in fish,two copies of cd4 genes,namely cd4-1 and cd4-2,are *** this study,the ectodomains of grass carp(Ctenopharyngodon idella,Ci)CD4-1 and CD4-2 were expressed in the *** cells and used to generate monoclonal antibodies in *** blotting,confocal microscopy and flow cytometry were performed to characterize the monoclonal *** has been shown that the CiCD4-1 and CiCD4-2 monoclonal antibodies had good specificity to react with the recombinant ectodomains of CiCD4-1 and CiCD4-2 expressed in the CHO-S cells and the native CD4 molecules of grass *** CiCD4-1 monoclonal antibody did not recognize CiCD4-2 and verse *** addition,the CD4-1 and CD4-2 monoclonal antibodies specifically recognized the CD4-1 and CD4-2 receptors expressed in the HEK293 cells and native molecules of fish ***,the percentages of lymphocytes in immune tissues of healthy fish were analyzed by flow *** was found that 17.6%of lymphocytes were CD4-1^(+)cells and 22.5%were CD4-2^(+)in the head *** the spleen,13.1%of lymphocytes were CD4-1^(+)and 18.6%were CD4-2^(+)while 7.3%of blood lymphocytes were CD4-1^(+)cells and 8.8%were CD4-2^(+)*** availability of the CD4-1 and CD4-2 monoclonal antibodies provides antibody-based tools for further elucidation of the functions of CD4 T cells in grass carp.

关键词： Grass carp CD4 Monoclonal antibodies Flow cytometry Confocal microscopy

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A direct electrochemical biosensor for rapid glucose detection based on nitrogen-doped carbon nanocages

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Rare Metals 2024年第5期43卷 2184-2192页

作者： Lan-Lan Li Yu zhao Li-jia Pan jian-Bin Xu Yi Shi College of Mechanical and Electrical Engineering Henan Agricultural UniversityZhengzhou 450002China School of Electronic Science and Engineering Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing210093China Department of Electronic Engineering The Chinese University of HongKongHongkong 999077China

Given the increasing number of diabetic patients,rapid and accurate detection of glucose in body fluids is *** study developed a direct electrochemical biosensor for glucose based on nitrogen-doped carbon nanocages(NCNCs).NCNCs possess a large specific surface area of 1395 m^(2)·g^(-1),a high N atomic content of 9.37%and good biocompatibility,which is favorable for enzyme loading and electron *** surface average concentration of electroactive glucose oxidase on NCNCs was 2.82×10^(-10)mol·cm^(-2).The NCNC-based direct electrochemical biosensor exhibited a high sensitivity of 13.7μA·(mmol·L^(-1))^(-1)·cm^(-2),rapid response time of 5 s and an impressive electron-transferrate constant(ks)of 1.87 s^(-1).Furthermore,we investigated an NCNC-based direct electron transfer(DET)biosensor for sweat glucose detection,which demonstrated tremendous promise for non-invasive wearable diabetes diagnosis.

关键词： Direct electron transfer(DET) Nitrogen-doped carbon nanocages(NCNCs) Glucose oxidase Biosensor

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A post-modification strategy to precisely construct dual-atom sites for oxygen reduction electrocatalysis

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Journal of Energy Chemistry 2024年第3期90卷 511-517,I0012页

作者： Juan wang Xinyan Liu Chang-Xin zhao Yun-Wei Song jia-Ning Liu Xi-Yao Li Chen-Xi Bi Xin Wan jianglan Shui Hong-Jie Peng Bo-Quan Li jia-Qi Huang School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing 100081China Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijing 100084China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 611731SichuanChina School of Materials Science and Engineering Beihang UniversityBeijing 100191China

Dual-atom catalysts(DACs) afford promising potential for oxygen reduction electrocatalysis due to their high atomic efficiency and high intrinsic ***,precise construction of dual-atom sites remains a *** this work,a post-modification strategy is proposed to precisely fabricate DACs for oxygen reduction ***,a secondary metal precursor is introduced to the primary single-atom sites to introduce direct metal-metal interaction,which ensures the formation of desired atom pair structure during the subsequent pyrolysis process and allows for successful construction of *** as-prepared FeCo-NC DAC exhibits superior oxygen reduction electrocatalytic activity with a half-wave potential of 0,91 V *** hydrogen ***-air batteries equipped with the FeCo-NC DAC demonstrate higher peak power density than those with the Pt/C *** importantly,this post-modification strategy is demonstrated universal to achieve a variety of dual-atom *** work presents an effective synthesis methodology for precise construction of catalytic materials and propels their applications in energy-related devices.

关键词： Dual-atom catalysts Electrocatalysis Oxygen reduction reaction Post-modification Zinc–air batteries

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Spatial transcriptomics:recent developments and insights in respiratory research

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Military Medical Research 2024年第3期11卷 430-448页

作者： Wen-jia wang Liu-Xi Chu Li-Yong He Ming-Jing Zhang Kai-Tong Dang Chen Gao Qin-Yu Ge Zhou-Guang wang Xiang-Wei zhao State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhou 325035ZhejiangChina Orthopaedic Bioengineering Research Group Division of Surgery and Interventional ScienceUniversity College LondonLondon HA74LPUK

The respiratory system's complex cellular heterogeneity presents unique challenges to researchers in this *** bulk RNA sequencing and single-cell RNA sequencing(scRNA-seq)have provided insights into cell types and heterogeneity in the respiratory system,the relevant specific spatial localization and cellular interactions have not been clearly *** transcriptomics(ST)has filled this gap and has been widely used in respiratory *** review focuses on the latest iterative technology of ST in recent years,summarizing how ST can be applied to the physiological and pathological processes of the respiratory system,with emphasis on the ***,the current challenges and potential development directions are proposed,including high-throughput full-length transcriptome,integration of multi-omics,temporal and spatial omics,bioinformatics analysis,*** viewpoints are expected to advance the study of systematic mechanisms,including respiratory studies.

关键词： Spatial transcriptomics Lung Tumor Spatial multi-omics

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