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Antiparasitic activities of specific bacterial extracellular products of Streptomyces griseus SDX-4 against Ichthyophthirius multifiliis

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Antiparasitic activities of specific bacterial extracellular...

2014年中国水产学会学术年会

作者： jia-yun yao Wen-Lin YIN Yang XU Wei-Ming AI Jin-Yu SHEN Zhejiang Institute of Freshwater Fisheries

The ciliate Ichthyophthirius multifiliis is one of the most pathogenic parasites of fish maintained in captivity. In this study, effects of bacterial extracellular products of Streptomyces griseus SDX-4 against I. multifiliis were determined. The fermentation liquor of S. griseus was extracted successively in a separating funnel with petroleum ether, ethyl acetate, and n-butanol. In vitro assays revealed that the n-butanol extracts（NBu-E） and ethyl acetate（Eto-E） of S. griseus were observed to be more effictive against theronts than the other extracts with median effective concentration（EC50） values of 0.86 mg L-1 and 12.5 mg L-1, respectively, and significantly reduced the survival of the tomonts and the total number of theronts released by the tomonts（P＜0.05）. All encysted tomonts were killed when the concentration of NBu-E was 30.0 mg L-1. Results of in vivo test demonstrated that the number of I. multifiliis trophonts on the grasscarp treated with NBu-E was markedly lower compared to the control group at 11 days after exposed to theronts（p＜0.05）. In the control group, 100% mortality was observed owing to heavy I. multifiliis infection at 11 days after the exposure. On the other hand, only 9.5% mortality owing to parasite infection was recorded in the groups treated with the NBu-E（30 mg L-1）. The median lethal dose（LD50） of NBu-E for grasscarp was 152.4 mg L-1. Our results indicate that n-butanol extract of S. griseus will be useful in aquaculture for controlling I. multifiliis infections.

关键词： Ichthyophthirius multifiliis extracellular products Streptomyces griseus Antiparasitic

Pharmacokinetic Disposition of Streptomycin Sulfate in Japanese Eel (<i>Anguilla japonica</i>) after Oral and Intramuscular Administrations

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Pharmacology & Pharmacy 2012年第2期3卷 195-200页

作者： Ping He Jin-Yu Shen Wen-Lin Yin jia-yun yao Yang Xu Xiao-Yi Pan Gui-Jie Hao Zhejiang Institute of Freshwater Fisheries Huzhou China

Pharmacokinetics and residue elimination of streptomycin sulfate (STR) are important in the determination of optimal dosage regimens and in establishing safe withdrawal periods in farmed fishes. The pharmacokinetics of STR was studied after a single dose (50 mg/kg) of intramuscular (i.m.) or oral gavage (p.o.) administration to Japanese eel (Anguilla japonica) in freshwater at 25°C. Eight fish per sampling point were examined after treatment. Plasma and muscle were collected and analyzed by high-performance liquid chromatography (HPLC) method with 0.05 μg/ml detection limit. The data of pharmacokinetics conformed to the two-compartment open model for intramuscular and one-compartment open model for oral administrations. After intramuscular administration, the elimination half-life (t1/2β) was calculated to be 11.346 h, the maximum plasma concentration (Cmax) to be 29.524 μg/ml, the time to peak plasma streptomycin concentration (Tmax) to be 0.218 h, and the area under the plasma concentration-time curve (AUC) to be 90.206 μg/ml?h. Following p.o. administration, the corresponding estimates were 13.239 h, 0.346 μg/ml, 11.960 h, and 12.356 μg/ml?h. After intramuscular administration, a therapeutic concentration of the drug was maintained for 12 hours in the plasma, however, a therapeutic level could not be achieved after oral administration, and the results suggest that the drug can be used clinically by intramuscular administration against streptomycin susceptible systemic infections in Japanese eel.

关键词： Pharmacokinetic Streptomycin Sulfate Japanese Eel Intramuscular Oral Gavage

Live recombinant Lactococcus lactis vaccine expressing immobilization antigen(i-Ag) for protection against Ichthyophthirius multifiliis in goldfish

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Live recombinant Lactococcus lactis vaccine expressing immob...

第十二届浙江渔业科技论坛

作者： jia-yun yao Xue-Mei Yuan Yang XU Wen-Lin YIN Ling-yun LIN Xiao-Yi PAN Gui-lian WANG Chun-Feng Wang Jin-Yu SHEN Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of Fish Health and Nutrition of Zhejiang ProvinceZhejiang Institute of Freshwater Fisheries College of Animal Science and Technology Jilin Agricultural University

The parasite Ichthyophthirius multifiliis(Ich) has been reported in various freshwater fishes worldwide and results in severe losses to both food and aquarium fish *** strains have a number of properties that make them attractive candidates as delivery vehicles for the presentation to the mucosa of compounds with pharmaceutical interest,in particular ***,the present study was conducted to evaluate a live recombinant Lactococcus lactis vaccine expressing immobilization antigen(IAG-52 X) in protection against ***.A 1266 bp gene fragment containing a potential antigenic epitope of the 48 kDa immobilization antigen of *** was assembled from six synthetic ohgonucleotides and cloned into pSIP409 and electrotransformed into Lactobacillus plantarum *** recombinant vaccine candidate was then orally fed into *** expression of immune-related genes:complement component 3(C3),MHC I,IgM gene in blood from goldfish at different time points after immunization were *** fish were than challenged with a lethal dose of infectious *** cumulative mortality and relative percentage survival(RPS) were also *** results showed that the antibody level in the blood and skin of the immunized fish was statistically significant(P < 0.05) in relation to the control *** orally immunized with NC8-pSIP409-IAG-52 X had high serum antibody titers that ranged from 32 to256 after 28 d post immunization,while fish fed with NC8-pSIP409 or PBS had no detectable immobilizing antibody *** of IgM,C3,MHC I genes in the group immunized with IAG-52 X were significantly(P < 0.05) up regulated as compared with control group,indicating that different immune cells were actively involved in cellular immune *** results showed that the average survival rate and RPS of fish orally immunized with 10 NC8-pSIP409-IAG-52 X was 50%and 55.5 ***,NC8-pSIP409-IAG-52 X could

关键词： Ichthyophthirius multifiliis Immune protection oral vaccines immobilization antigen gene expression

Transcriptomic profiling reveals new insights into the regulation of immune pathways during largemouth bass virus(LMBV) infection

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Transcriptomic profiling reveals new insights into the regul...

浙江省免疫学会第十三次学术大会

作者： Jing Chen Xiao-Hong Huang Jin-Biao jiao Xue-Mei Yuan Lei Huang Zhe Yu Wen-Lin Yin Ling-yun Lin Xiao-Yi Pan Jin-Yu Shen Hai-Qi Zhang jia-yun yao Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of Fish Health and Nutrition of Zhejiang ProvinceZhejiang Institute of Freshwater Fisheries

Background:Largemouth bass virus（LMBV）,which belongs to the genus Ranavirus,causes high mortality and serious economic losses in the largemouth bass breeding ***:This study aims to investigate the immune regulatory mechanisms of largemouth bass induced by LMBV infection at different infection *** and methods:We conducted Hematoxylin-Eosin（HE） staining and transcriptome analysis for LMBV-infected largemouth bass with different time points in this ***:Hematoxylin-Eosin（HE） staining for liver and guts tissues indicated that the liver cells of largemouth bass were highly swollen,many round vacuoles were found in the cytoplasm,and intestinal villi increased,intestinal cavity expanded at 48 hours *** transcriptome analysis indicated that 150 differentially expressed genes（DEGs） uniquely existed in D-vs-H48,and 85 overlapped DEGs were detected in the six pairwise ***,a total of 1,895 differentially expressed transcription factors（TFs） were identified and classified into 69 TF families,among which zf-C2 H2（408）,Homeobox（291）,bHLH（167）,TFZIP（102） contained more genes than other TF families and involved in immune *** analysis indicated that the 1,631 DEGs of D-vs-H48 were enriched in 316 pathways,including Cytokine-cytokine receptor interaction（ko04060）,JAK-STAT signaling pathway（ko04630）,TNF signaling pathway（ko04668）,***,we explored the time-process differential expression of 53 genes annotated in cytokine-cytokine receptor *** result revealed that D,H4,and H72 groups were clustered into one branch with a similar expression level,and H24 and H48 groups were clustered into one branch with a similar expression ***,a total of 9 genes showed a high expression level in D,H4,and H72 groups compared to H24 and H48 groups;44 genes had a low expression level in D,H4,and H72 groups,and a high expression level in H24 and H48 *** up-regulated genes mainly

关键词： Largemouth bass virus transcriptome analysis histological structure innate immune system cytokine

Evaluation of Salinomycin isolated from Streptomyces albus JSY-2 against the ciliate,Ichthyophthirius multifiliis

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Evaluation of Salinomycin isolated from Streptomyces albus J...

2018年（第十三届）浙江渔业科技论坛

作者： jia-yun yao Ming-Yue Gao Yong-Yi jia Yan-Xia WU Wen-Lin YIN Zheng CAO Gui-Lian YANG Hai-Bin HUANG Chun-Feng WANG Jin-Yu SHEN Zhi-Min GU College of Animal Science and Technology Jilin Provincial Engineering Research Center of Animal ProbioticsKey Laboratory of animal production and product quality safety of Ministry of Education Jilin Agricultural University Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of Fish Health and Nutrition of Zhejiang Province Zhejiang Institute of Freshwater Fisheries

The present study was undertaken to investigate the antiparasitic activity of extracellular products of Streptomyces ***-guided isolation of chloroform extracts affording a compound showing potent *** structure of tihe compound was elucidated as Salinomycin(SAL)by EI-MS, H NMR and C *** vitro test showed that SAL has potent anti-parasitic efficacy against theronts of *** with 10 min-,1 h-,2 h-,3 h-and 4 h-(effective concentration)EC(95% Confidence Intervals)of 2.12(2.22-2.02),1.93(1.98-1.88),1.42(1.47-1.37),1.35(1.41-1.31)and1.11(1.21-1.01)mg *** vitro antiparasitic assays revealed that SAL could be 100% effective against I multifiliis encysted tomonts at a concentration of 8.0 mg *** vivo test demonstrated that number of *** trophonts on Erythroculter ilishaeformis treated with SAL was markedly lower than that of control group at 10 days after exposed to theronts(p<0.05).In the control group,80% mortality was observed owing to heavy L multifiliis infection at 10 *** the other hand,only 30.0 % mortality was recorded in the group treated with 8.0 mg *** median lethal dose(LD)of SAL for *** was 32.9 mg L.

关键词： Ichthyophthirius multifiliis Salinomycin Sterptomyces albus Antiparasitic

Effects of ultrasonic-assisted extraction on bioactive compounds,volatile flavors and antioxidant activities of vine tea water extracts

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Traditional Medicine Research 2025年第1期10卷 73-81页

作者： Xiao-Long Zhou Wei-Jin jiang Ji Yu Mao-Jun yao yun-Tong Li Hunan Provincial Key Laboratory of Research Resource Mining and High-valued Utilization on Edible&Medicinal PlantCollege of Chemistry and Chemical EngineeringJishou UniversityJishou 416000China Hunan Gaoge Dairy Co. Ltd.Changsha 410000China

Background:Ampelopsis grossedentata,vine tea,which is the tea alternative beverages in *** vine tea processing,a large amount of broken tea is produced,which has low commercial ***:This study investigates the influence of different extraction methods(room temperature water extraction,boiling water extraction,ultrasonic-assisted room temperature water extraction,and ultrasonic-assisted boiling water extraction,referred to as room temperature water extraction(RE),boiling water extraction(BE),ultrasonic assistance at room temperature water extraction(URE),and ultrasonic assistance in boiling water extraction(UBE))on the yield,dihydromyricetin(DMY)content,free amino acid composition,volatile aroma components,and antioxidant properties of vine tea ***:A notable influence of extraction temperature on the yield of vine tea extracts(P<0.05),with BE yielding the highest at 43.13±0.26%,higher than that of RE(34.29±0.81%).Ultrasound-assisted extraction significantly increased the DMY content of the extracts(P<0.05),whereas DMY content in the RE extracts was 59.94±1.70%,that of URE reached 66.14±2.78%.Analysis revealed 17 amino acids,with L-serine and aspartic acid being the most abundant in the extracts,nevertheless ultrasound-assisted extraction reduced total free amino acid *** chromatography-mass spectrometry analysis demonstrated an increase in the diversity and quantity of compounds in the vine tea water extracts obtained through ultrasonic-assisted ***,69 and 68 volatile compounds were found in URE and UBE extracts,which were higher than the number found in RE and BE *** vitro,antioxidant activity assessments revealed varying antioxidant capacities among different extraction methods,with RE exhibiting the highest DPPH scavenging rate,URE leading in ABTS•+free radical scavenging,and BE demonstrating superior ferric ion reducing antioxidant ***:The findings suggest that extraction methods signi

关键词： vine tea dihydromyricetin ultrasonic-assisted extraction volatile aroma components

Nanostructured ZnO/BiVO_(4)I-scheme heterojunctions for piezocatalytic degradation of organic dyes via harvesting ultrasonic vibration energy

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 488-497页

作者： Yiling Li Xiaoyao Yu Yingjie Zhou yao Lin Ying Wu Key Laboratory of the Ministry of Education for Advanced Catalysis Materials College of Chemistry and Materials ScienceZhejiang Normal UniversityJinhua 321004China

BiVO_(4)porous spheres modified by ZnO were designed and synthesized using a facile two-step *** resulting ZnO/BiVO_(4)composite catalysts have shown remarkable efficiency as piezoelectric catalysts for degrading Rhodamine B(RhB)unde mechanical vibrations,they exhibit superior activity compared to pure *** 40wt%ZnO/BiVO_(4)heterojunction composite displayed the highest activity,along with good stability and *** enhanced piezoelectric catalytic activity can be attributed to the form ation of an I-scheme heterojunction structure,which can effectively inhibit the electron-hole ***,hole(h+)and superoxide radical(·O_(2)^(-))are proved to be the primary active ***,ZnO/BiVO_(4)stands as an efficient and stable piezoelectric catalyst with broad potential application in the field of environmental water pollution treatment.

关键词： piezoelectric catalytic heterojunction dye degradation ultrasonic vibration

维普期刊数据库

基于Workbench刀砧的热-结构耦合分析

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锻压装备与制造技术 2018年第2期53卷 75-79页

作者：张鸿飞尹华贾鋆贾晓亮姚鹤兰州兰石能源装备工程研究院有限公司甘肃兰州730050 甘肃省金属塑性成型装备智能控制重点实验室甘肃兰州730050 甘肃省大型快锻液压设备工程技术研究中心甘肃兰州730050 兰州兰石检测技术有限公司甘肃兰州730050

本文首先通过Solidworks建立了刀砧三维参数模型,基于workbench软件对其进行了静力学结构分析,得出了其应力、应变及方向变形的情况;其次,基于workbench对刀砧进行稳态热分析,然后利用顺序耦合的方法,把刀砧热分析得出的温度场以体积力... 详细信息

本文首先通过Solidworks建立了刀砧三维参数模型,基于workbench软件对其进行了静力学结构分析,得出了其应力、应变及方向变形的情况;其次,基于workbench对刀砧进行稳态热分析,然后利用顺序耦合的方法,把刀砧热分析得出的温度场以体积力形式导入静态结构中进行热-结构耦合分析,最后得出了刀砧在热-结构耦合应力作用下的应力、应变及其方向变形,结果表明热应力对刀砧的应力、应变及方向变形影响较大。

关键词：刀砧 Workbench 热-结构耦合分析应力应变

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Highly conductive and stable iodine doped argyrodite electrolyte for all-solid-state lithium batteries

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Journal of Energy Chemistry 2025年第1期100卷 50-58页

作者： Gaozhan Liu Jing Zhang Jing Yang Jinghui Chen Xingyue Xiao Xiayin yao Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences

Lithium argyrodites with high ionic conductivity and low cost are considered as one of the most prospective solid electrolytes for all-solid-state lithium ***,the poor chemical stability and compatibility with lithium metal limit their ***,Li5.4PS4.4Cl1.4I0.2solid electrolyte with high ionic conductivity of 11.49 m S cm-1and improved chemical stability is synthesized by iodine *** ultra-thin Li5.4PS4.4Cl1.4I0.2membrane with thickness of 10μm can be obtained by wet coating process,exhibiting a high ionic conductivity of 2.09 mS cm-1and low areal resistance of 1.17Ω***,iodine doping could in-situ form LiI at the lithium/electrolyte interface and improve the critical current density of Li5.4PS4.4Cl1.6from 0.8 to 1.35 mA *** resultant LiCoO2/Li5.4PS4.4Cl1.4I0.2/Li battery shows excellent cycling stability at 1 C,with a reversible specific capacity of 110.1 mA h g-1and a retention of 80.5% after 1000 *** addition,the assembled LiCoO2/Li5.4PS4.4Cl1.4I0.2membrane/Li pouch cell delivers an initial discharge capacity of 110.4 mA h g-1and 80.5% capacity retention after 100 cycles.

关键词： Argyrodite electrolytes Iodine doping Ionic conductivity Stability All-solid-state lithium battery