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Hepatocyte growth factor enhances the ability of dental pulp stem cells to ameliorate atherosclerosis in apolipoprotein E-knockout mice

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World Journal of Stem Cells 2024年第5期16卷 575-590页

作者： Han Duan Ning Tao Lin Lv Kai-xin Yan Yong-Gang You Zhuang Mao Chang-Yao wang Xue Li jia-Yan Jin Chu-Tse Wu Hua wang School of Life Sciences Hebei UniversityBaoding 071002Hebei ProvinceChina Beijing Institute of Radiation Medicine Beijing 100850China Department of Cardiology The Sixth Medical CentreChinese People’s Liberation Army General HospitalBeijing 100037China Department of Orthopaedics The Fourth Medical CentreChinese People’s Liberation Army General HospitalBeijing 100853China Third Cadet Regiment School of Basic Medical ScienceAir Force Medical UniversityXi’an 710032Shaanxi ProvinceChina

BACKGROUND Atherosclerosis(AS),a chronic inflammatory disease of blood vessels,is a major contributor to cardiovascular *** pulp stem cells(DPSCs)are capable of exerting immunomodulatory and anti-inflammatory effects by secreting cytokines and exosomes and are widely used to treat autoimmune and inflam-mation-related *** growth factor(HGF)is a pleiotropic cytokine that plays a key role in many inflammatory and autoimmune *** To modify DPSCs with HGF(DPSC-HGF)and evaluate the therapeutic effect of DPSC-HGF on AS using an apolipoprotein E-knockout(ApoE-/-)mouse model and an in vitro cellular *** ApoE-/-mice were fed with a high-fat diet(HFD)for 12 wk and injected with DPSC-HGF or Ad-Null modified DPSCs(DPSC-Null)through tail vein at weeks 4,7,and 11,respectively,and the therapeutic efficacy and mechanisms were analyzed by histopathology,flow cytometry,lipid and glucose measurements,real-time reverse transcription polymerase chain reaction(RT-PCR),and enzyme-linked immunosorbent assay at the different time points of the *** in vitro inflammatory cell model was established by using RAW264.7 cells and human aortic endothelial cells(HAOECs),and indirect co-cultured with supernatant of DPSC-Null(DPSC-Null-CM)or DPSC-HGF-CM,and the effect and mechanisms were analyzed by flow cytometry,RT-PCR and western *** factor-κB(NF-κB)activators and inhibitors were also used to validate the related signaling *** DPSC-Null and DPSC-HGF treatments decreased the area of atherosclerotic plaques and reduced the expression of inflammatory factors,and the percentage of macrophages in the aorta,and DPSC-HGF treatment had more pronounced *** treatment had no effect on serum lipoprotein *** FACS results showed that DPSCs treatment reduced the percentages of monocytes,neutrophils,and M1 macrophages in the peripheral blood and ***-Null-CM and DPSC-HGF-CM reduced adhesion molecule expression in tu

关键词： Atherosclerosis Apolipoprotein E-knockout mice Cell therapy Dental pulp stem cells Hepatocyte growth factor

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Risk factors and clinical significance of posterior slip of the proximal vertebral body after lower lumbar fusion

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World Journal of Clinical Cases 2024年第26期12卷 5885-5892页

作者： jia-Jun Zhu Yi wang Jun Zheng Sheng-Yang Du Lei Cao Yu-Ming Yang Qing-xi Zhang Ding-Ding xie Department of Spinal Surgery Xuzhou First People’s HospitalXuzhou 221112Jiangsu ProvinceChina Department of Spinal Surgery The Affiliated Xuzhou Municipal Hospital of Xuzhou Medical UniversityXuzhou 221112Jiangsu ProvinceChina Department of Spinal Surgery The Affiliated Hospital of China University of Mining and TechnologyXuzhou 221112Jiangsu ProvinceChina

BACKGROUND Adjacent segment disease(ASD)after fusion surgery is frequently manifests as a cranial segment instability,disc herniation,spinal canal stenosis,spondylolisthesis or *** risk factors and mechanisms of ASD have been widely discussed but never clearly *** To investigate the risk factors and clinical significance of retrograde movement of the proximal vertebral body after lower lumbar *** This was a retrospective analysis of the clinical data of patients who underwent transforaminal lumbar interbody fusion surgery between September 2015 and July 2021 and who were followed up for more than 2 ***-one patients with degenerative lumbar diseases were included(22 males and 69 females),with an average age of 52.3 years(40-73 years).According to whether there was retrograde movement of the adjacent vertebral body on postoperative X-rays,the patients were divided into retrograde and nonretrograde *** sagittal parameters of the spine and pelvis were evaluated before surgery,after surgery,and at the final *** the same time,the Oswestry Disability Index(ODI)and Visual Analogue Scale(VAS)were used to evaluate the patients’quality of *** Nineteen patients(20.9%)who experienced retrograde movement of proximal adjacent segments were included in this *** pelvic incidence(PI)of the patients in the retrograde group were significantly higher than those of the patients in the nonretrograde group before surgery,after surgery and at the final follow-up(P<0.05).There was no significant difference in lumbar lordosis(LL)between the two groups before the operation,but LL in the retrograde group was significantly greater than that in the nonretrograde group postoperatively and at the final *** significant differences were detected in terms of the|PI–LL|,and there was no significant difference in the preoperative lordosis distribution index(LDI)between the two *** LDIs of the retrograde group were

关键词： Adjacent segment disease Posterior vertebral slip Sagittal alignment of spine-pelvis Lower lumbar fusion Quality of life

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一种迭代边界优化的医学图像小样本分割网络

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自动化学报 2024年第10期50卷 1988-2001页

作者：贾熹滨郭雄王珞杨大为杨正汉北京工业大学信息学部北京100124 首都医科大学附属北京友谊医院北京100050

精准的医学图像自动分割是临床影像学诊断和影像三维重建的重要基础.但医学图像数据的目标对象间对比度差异小、受器官运动影响大,加之标注样本规模小,因此在小样本下建立高性能的医学分割模型仍是目前的难点问题.针对主流原型学习小样... 详细信息

精准的医学图像自动分割是临床影像学诊断和影像三维重建的重要基础.但医学图像数据的目标对象间对比度差异小、受器官运动影响大,加之标注样本规模小,因此在小样本下建立高性能的医学分割模型仍是目前的难点问题.针对主流原型学习小样本分割网络对医学图像边界分割性能差的问题,提出一种迭代边界优化的小样本分割网络(Iterative boundary refinement based few-shot segmentation network,IBR-FSS-Net).以双分支原型学习的小样本分割框架为基础,引入类别注意力机制和密集比较模块(Dense comparison module,DCM),对粗分割掩码进行迭代优化,引导分割模型在多次迭代学习过程中关注边界,从而提升边界分割精度.为进一步克服医学图像训练样本少且多样性不足问题,使用超像素方法生成伪标签,扩充训练数据以提升模型泛化性.在ABD-MR和ABD-CT医学图像分割公共数据集上进行实验,与现有多种先进的医学图像小样本分割方法进行对比分析和消融实验.实验结果表明,该方法有效提升了未见医学类别的分割性能.

关键词：医学图像分割小样本学习注意力机制边界优化

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The energy response of LaBr_(3)(Ce),LaBr_(3)(Ce,Sr),and NaI(Tl)crystals for GECAM

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Nuclear Science and Techniques 2024年第2期35卷 110-122页

作者： Pei-Yi Feng xi-Lei Sun Zheng-Hua An Yong Deng Cheng-Er wang Huang jiang Jun-Jie Li Da-Li Zhang xin-Qiao Li Shao-Lin xiong Chao Zheng Ke Gong Sheng Yang xiao-Jing Liu Min Gao xiang-Yang Wen Ya-Qing liu Yan-Bing Xu xiao-Yun Zhao jia-Cong Liu Fan Zhang Hong Lu Particle and Astrophysics Center Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100049China Experimental Physics Center Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China School of Nuclear Science and Technology University of South ChinaHengyang 421001China National Engineering Research Center for Rare Earth Grirem Advanced Materials Co.Ltd.and General Research Institute for Nonferrous MetalsBeijing 100088China

The GECAM series of satellites utilizes LaBr_(3)(Ce),LaBr_(3)(Ce,Sr),and NaI(Tl)crystals as sensitive materials for gamma-ray detectors(GRDs).To investigate the nonlinearity in the detection of low-energy gamma rays and address the errors in the calibration of the E-C relationship,comprehensive tests and comparative studies of the three aforementioned crystals were conducted using Compton electrons,radioactive sources,and mono-energetic *** nonlinearity test results of the Compton electrons and X-rays demonstrated substantial differences,with all three crystals presenting a higher nonlinearity for X/-rays than for Compton *** the LaBr_(3)(Ce)and LaBr_(3)(Ce,Sr)crystals having higher absolute light yields,they exhibited a noticeable nonlinear decrease in the light yield,especially at energies below 400 *** NaI(Tl)crystal demonstrated an"excess"light output in the 6-200 keV range,reaching a maximum"excess"of 9.2%at 30 keV in the X-ray testing and up to 15.5%at 14 keV during Compton electron testing,indicating a significant advantage in the detection of low-energy gamma ***,we explored the underlying causes of the observed nonlinearity in these *** study not only elucidates the detector responses of GECAM,but also initiates a comprehensive investigation of the nonlinearity of domestically produced lanthanum bromide and sodium iodide crystals.

关键词： LaBr_(3)(Ce)detector LaBr_(3)(Ce,Sr)detector NaI(Tl)detector GECAM Energy response Light yield nonlinearity

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The issues on the commercialization of perovskite solar cells

Materials Futures

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Materials Futures 2024年第2期3卷 1-35页

作者： Lixiu Zhang Yousheng wang xiangchuan Meng jia Zhang Pengfei Wu Min wang Fengren Cao Chunhao Chen Zhaokui wang Fu Yang xiaodong Li Yu Zou xi Jin Yan jiang Hengyue Li Yucheng Liu Tongle Bu Buyi Yan Yaowen Li Junfeng Fang Lixin xiao Junliang Yang Fuzhi Huang Shengzhong Liu Jizhong Yao Liangsheng Liao Liang Li Fei Zhang Yiqiang Zhan Yiwang Chen Yaohua Mai Liming Ding Center for Excellence in Nanoscience(CAS) Key Laboratory of Nanosystem and Hierarchical Fabrication(CAS)National Center for Nanoscience and TechnologyBeijing 100190People’s Republic of China Institute of New Energy Technology College of Information Science and TechnologyJinan UniversityGuangzhou 510632People’s Republic of China Institute of Polymers and Energy Chemistry(IPEC) Nanchang UniversityNanchang 330031People’s Republic of China School of Information Science and Technology Fudan UniversityShanghai 200433People’s Republic of China School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072People’s Republic of China School of Physical Science and Technology Soochow UniversitySuzhou 215006People’s Republic of China Institute of Functional Nano&Soft Materials(FUNSOM) Soochow UniversitySuzhou 215123People’s Republic of China Institute of optoelectronics Fudan UniversityShanghai 200433People’s Republic of China College of Chemistry Chemical Engineering and Materials ScienceSoochow UniversitySuzhou 215123People’s Republic of China School of Physics and Electronic Science East China Normal UniversityShanghai 200241People’s Republic of China State Key Laboratory for Artificial Microstructure and Mesoscopic Physics Department of PhysicsPeking UniversityBeijing 100871People’s Republic of China College of Biophotonics South China Normal UniversityGuangzhou 510631People’s Republic of China School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081People’s Republic of China School of Physics and Electronics Central South UniversityChangsha 410083People’s Republic of China School of Materials Science and Engineering Shaanxi Normal UniversityXi’an 710119People’s Republic of China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070People’s Republic of China Microquanta semiconductor Hangzhou 311121People’s Republic of China

Perovskite solar cells have aroused a worldwide research upsurge in recent years due to their soaring photovoltaic performance,ease of solution processing,and low *** power conversion efficiency record is constantly being broken and has recently reached 26.1%in the lab,which is comparable to the established photovoltaic technologies such as crystalline silicon,copper indium gallium selenide and cadmium telluride(CdTe)solar ***,perovskite solar cells are standing at the entrance of industrialization,where huge opportunities and risks ***,towards commercialization,challenges of up-scaling,stability and lead toxicity still remain,the proper handling of which could potentially lead to the widespread adoption of perovskite solar cells as a low-cost and efficient source of renewable *** review gives a holistic analysis of the path towards commercialization for perovskite solar cells.A comprehensive overview of the current state-of-the-art level for perovskite solar cells and modules will be introduced first,with respect to the module efficiency,stability and current status of *** will then discuss the challenges that get in the way of commercialization and the corresponding strategies to address them,involving the upscaling,the stability and the lead toxicity *** into the future direction of commercialization of perovskite photovoltaics was also provided,including the flexible perovskite cells and modules and perovskite indoor ***,the future perspectives towards commercialization are put forward.

关键词： commercializations perovskite solar cells state-of-the-art level challenges and perspectives indoor photovoltaics

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Myotis bat STING attenuates aging-related inflammation in female mice

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动物学研究 2024年第5期 961页

作者： xi wang Jing-Kun jia Qi wang Jing-Wen Gong Ang Li jia Su Peng Zhou Wuhan Institute of Virology Chinese Academy of Sciences Guangzhou National Laboratory Guangzhou International Bio Island University of Chinese Academy of Sciences State Key Laboratory of Respiratory Disease First Affiliated Hospital of Guangzhou Medical School

Bats, notable as the only flying mammals, serve as natural reservoir hosts for various highly pathogenic viruses in humans(e.g., SARS-CoV and Ebola virus). Furthermore,bats exhibit an unparalleled longevity among mammals relative to their size, particularly the Myotis bats, which can live up to 40 years. However, the mechanisms underlying these distinctive traits remain incompletely understood. In our prior research, we demonstrated that bats exhibit dampened STING-interferon activation, potentially conferring upon them the capacity to mitigate virus-or aging-induced inflammation. To substantiate this hypothesis, we established the first in vivo bat-mouse model for aging studies by integrating Myotis davidii bat STING(Md STING) into the mouse genome. We monitored the genotypes of these mice and performed a longitudinal comparative transcriptomic analysis on Md STING and wild-type mice over a 3-year aging process. Blood transcriptomic analysis indicated a reduction in agingrelated inflammation in female Md STING mice, as evidenced by significantly lower levels of pro-inflammatory cytokines and chemokines, immunopathology, and neutrophil recruitment in aged female Md STING mice compared to aged wild-type mice in vivo. These results indicated that Md STING knock-in attenuates the agingrelated inflammatory response and may also improve the healthspan in mice in a sex-dependent manner. Although the underlying mechanism awaits further study, this research has critical implications for bat longevity research, potentially contributing to our comprehension of healthy aging in humans.

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The chromosome-scale assembly of the Notopterygium incisum genome provides insight into the structural diversity of coumarins

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3760-3773页

作者： Qien Li Yiqun Dai xin-Cheng Huang Lanlan Sun Kaixuan wang xiao Guo Dingqiao Xu Digao Wan Latai An Zixuan wang Huanying Tang Qi Qi Huihui Zeng Minjian Qin jia-Yu Xue Yucheng Zhao Tibetan Medicine Research Center of Qinghai University Tibetan Medical CollegeQinghai UniversityXining 810016China School of Pharmacy Bengbu Medical UniversityBengbu 233030China Department of Resources Science of Traditional Chinese Medicines School of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjing 210009China College of Horticulture Bioinformatics CenterAcademy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjing 210095China Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility Shaanxi University of Chinese MedicineXi’an 712046China

Coumarins,derived from the phenylpropanoid pathway,represent one of the primary metabolites found in *** alignment of the tetrahydropyran(THP)and tetrahydrofuran(THF)rings with the lactone structure results in the formation of at least four types of complex ***,the mechanisms underlying the structural diversity of coumarin remain poorly ***,we report the chromosome-level genome assembly of Notopterygium incisum,spanning 1.64 Gb,with a contig N50 value of 22.7 Mb and 60,021 annotated protein-coding ***,we identified the key enzymes responsible for shaping the structural diversity of coumarins,including two p-coumaroyl CoA 2′-hydroxylases crucial for simple coumarins basic skeleton architecture,two UbiA prenyltransferases responsible for angular or linear coumarins biosynthesis,and five CYP736 cyclases involved in THP and THF ring ***,two bifunctional enzymes capable of catalyzing both demethylsuberosin and osthenol were identified for the first *** analysis implies that tandem and ectopic duplications of the CYP736 subfamily,specifically arising in the Apiaceae,contributed to the structural diversity of coumarins in ***,this study proposes a parallel evolution scenario for the complex coumarin biosynthetic pathway among different angiosperms and provides essential synthetic biology elements for the heterologous industrial production of coumarins.

关键词： Notopterygium incisum Parallel evolution Biosynthesis Pyranocoumarins Furanocoumarins

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Joint alignment and steering to manage interference

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Digital Communications and Networks 2024年第2期10卷 429-438页

作者： Zhao Li xiujuan Liang Yinghou Liu jia Liu Zheng Yan School of Cyber Engineering Xidian UniversityXi'an710126China Technol.on Commun.Net.Lab. The 54th Research Inst.of China Elec.Technol.Group Corp.Shijiazhuang050081China School of Computer Science and Technology Xidian UniversityXi'an710126China The Department of Communications and Networking(Comnet) Aalto University02150EspooFinland

In wireless communication networks,mobile users in overlapping areas may experience severe interference,therefore,designing effective Interference Management(IM)methods is crucial to improving network ***,when managing multiple disturbances from the same source,it may not be feasible to use existing IM methods such as Interference Alignment(IA)and Interference Steering(IS)*** is because with IA,the aligned interference becomes indistinguishable at its desired Receiver(Rx)under the cost constraint of Degrees-of-Freedom(DoF),while with IS,more transmit power will be consumed in the direct and repeated application of IS to each *** remedy these deficiencies,Interference Alignment Steering(IAS)is proposed by incorporating IA and IS and exploiting their advantages in *** IAS,the interfering Transmitter(Tx)first aligns one interference incurred by the transmission of one data stream to a one-dimensional subspace orthogonal to the desired transmission at the interfered Rx,and then the remaining interferences are treated as a whole and steered to the same subspace as the aligned ***,two improved versions of IAS,i.e.,IAS with Full Adjustment at the Interfering Tx(IAS-FAIT)and Interference Steering and Alignment(ISA),are *** former considers the influence of IA on the interfering user-pair's *** orthogonality between the desired signals at the interfered Rx can be maintained by adjusting the spatial characteristics of all interferences and the aligned interference components,thus ensuring the Spectral Efficiency(SE)of the interfering communication *** ISA,the power cost for IS at the interfered Tx is minimized,hence improving SE performance of the interfered *** the proposed methods are realized at the interfering and interfered Txs cooperatively,the expenses of IM are shared by both *** in-depth simulation results show that joint use of IA and

关键词： Interference Interference management Interference alignment Interference steering Spectral efficiency

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Strength and damage evolution mechanism of rock mass with holes under cyclic loading

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Journal of Central South University 2024年第8期31卷 2717-2735页

作者： Liu, Hong-tao Han, Zi-jun Guo, xiao-fei Liu, Qin-yu Qiao, Zhong-jin Liang, jia-lu Cheng, Wen-cong Zhang, xi-ying Zhang, Yu-qi School of Energy and Mining Engineering China University of Mining and Technology (Beijing) Beijing China Research Center of Roadway Support and Surrounding Rock Control Engineering in Coal Industry Beijing China

The damage and failure law of rock mass with holes is of great significance to the stability control of roadways. This study investigates the mechanical properties and failure modes of porous rock masses under cyclic loading, elucidates the acoustic emission (AE) characteristics and their spatial evolution, and establishes the interrelation among AE, stress, strain, time, and cumulative damage. The results reveal that the rock mass with holes and the intact rock mass show softening and hardening characteristics after cyclic loading. The plastic strain of the rock mass with holes is smaller than that of the intact rock mass, and the stress - strain curve shows hysteresis characteristics. Under uniaxial compression, the pore-bearing rock mass shows the characteristics of higher ringing count, AE energy, b-value peak, and more cumulative ringing count in the failure stage, while it shows lower characteristics under cyclic action. At the initial stage of loading, compared with the intact rock mass, the pore-containing rock mass shows the characteristics of a low b-value. The AE positioning and cumulative damage percentage are larger, and the AE positioning is denser around the hole. The specimen with holes is mainly shear failure, and the complete specimen is mainly tensile shear failure.

关键词： roadway surrounding rock control acoustic emission cyclic loading failure mode precursor of destruction

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Effects of Heterogeneous Polyhedrin on Virion Occlusion and Assembly of Fusion Protein

Effects of Heterogeneous Polyhedrin on Virion Occlusion and ...

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华东六省一市生物化学与分子生物学会2008年学术交流会

作者： HUANG jia-xi wang Wen-Bing WU Yah LI Bing SHEN Wei-De (Institute of Life Sciences,jiangsu University,301 Xuefu Road,Zhenjiang 212013,P.R.China)

The role of polyhedrin in the occlusion of virions was studied by substituting heterologous polyhedrin-coding sequences from a Attacus ricini nuclear polyhedrosis virus (ArMNPV)into the genome of Bombyx mori nuclear polyhydrosis virus(BmMNPV).The heterologous polyhedrin gene was highly expressed and polyhedra was produced in the nuclei of cells infected with the recombinant *** produced by the recombinant containing the ArMNPV polyhedrin(Ar)showed abnormal morphology,being small cuboidal inclusions rather than large triangle in the nucleus of infected *** formation of small cuboidal polyhedra makes it clear that occlusion body shape is determined by more than the primary amino acid sequence *** addition,a fully functional enhanced green fluorescent protein(EGFP)-Ar chimera was synthesized in order to investigate intracellular localization of Ar and illustrate the assembly capability of a Ar molecule with a large carboxyl terminus *** presence of fluorescence polyhedra was confirmed by confocal *** results demonstrates that virion occlusion is a finely controlled process that is to some extent specific to the polyhedrin involved and may also require other factors for optimal morphogenesis of *** also opens up the possibility of application as a delivery system and fusion protein purification.

关键词： polyhedrin polyhedra fusion protein assembly

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