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Designer cell-self-implemented labeling of microvesicles in situ with the intracellular-synthesized quantum dots

Science China Chemistry 2020年第4期63卷 448-453页

作者： Ling-Hong Xiong jia-wei Tu Ya-nan Zhang Ling-Ling Yang Ran Cui Zhi-Ling Zhang Dai-Wen Pang College of Chemistry and Molecular Sciences State Key Laboratory of VirologyInstitute for Advanced Studiesand Wuhan Institute of BiotechnologyWuhan UniversityWuhan 430072China State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratoryof Biosensing and Molecular RecognitionResearch Center for Analytical Sciencesand College of ChemistryNankai UniversityTianjin 300071China

Cell-derived microvesicles(MVs) are secreted from almost all kinds of mammalian cells into the extracellular space, and play crucial roles in intercellular communication and transporting biomolecules between cells. However, there is a great challenge for visualizing and monitoring of MVs’ bio-behaviors due to the limitations of existing labeling methods. Herein, we report the first paradigm of designer cell-self-implemented labeling of MVs secreted from living mammalian MCF-7 cells in situ using the intracellular-synthesized fluorescent quantum dots(QDs) during the formation of MVs. By elaborately coupling intracellular biochemical reactions and metabolism pathways, the MCF-7 cells can be illuminated brightly by intracellular-biosynthesized fluorescent CdSe QDs. Simultaneously, intracellular-synthesized QDs can be in situ encapsulated by the secreted MVs budding from the plasma membrane of the fluorescing cells to label the MVs with an efficiency of up to 89.9%. The whole labeling process skillfully combines designer precise cell-tuned intricate synthesis of CdSe QDs with mild in-situ labeling via cell-selfimplementation just after feeding the cell with suitable chemicals, which is structure-or function-nondestructive and much more straightforward and milder than those by chemical conjugation or indirect encapsulation with conventional fluorogenic labels.

关键词： in situ labeling live cell synthesis cell-derived microvesicles quantum dot cell-self-implementation

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Modeling fluid–particle interaction in dilute-phase turbulentliquid–particle flow simulation

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Particuology 2010年第2期8卷 150-160页

作者： Kunn Hadinoto jia wei Chew School of Chemical and Biomedical Engineering Nanyang Technological University Singapore 637459 Singapore Department of Chemical and Biological Engineering University of Colorado Boulder CO 80309 USA

The present work examines the predictive capability of a two-fluid CFD model that is based on the kinetic theory of granular flow in simulating dilute-phase turbulent liquid-particle pipe flows in which the inter- stitial fluid effect on the particle fluctuating motion is significant. The impacts of employing different drag correlations and turbulence closure models to describe the fluid-particle interactions （i.e. drag force and long-range interaction） are examined at both the mean and fluctuating velocity levels. The model pre- dictions are validated using experimental data of turbulent liquid-particle flows in a vertical pipe at different particle Reynolds numbers （Rep 〉 400 and Rep 〈 400）, which characterize the importance of the vortex shedding phenomenon in the fluid-phase turbulence modulation. The results indicate that （1） the fluctuating velocity level predictions at different Rep are highly sensitive to the drag correlation selec- tion and （2） different turbulence closure models must be employed to accurately describe the long-range fluid-particle interaction in each phase. In general, good agreement is found between the model predic- tions and the experimental data at both the mean and fluctuating velocity levels provided that appropriate combinations of the drag correlation and the turbulence closure model are selected depending on Rep. 2009 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.

关键词： Slurry flowLiquid-particle flowKinetic theory of granular flowDrag correlationTurbulence

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High-voltage LiCoO_(2) cathodes for high-energy-density lithiumion battery

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Rare Metals 2022年第12期41卷 3946-3956页

作者： Jing-Chao Zhang Zhe-Dong Liu Cui-Hua Zeng jia-wei Luo Yi-Da Deng Xiao-Ya Cui Ya-nan Chen School of Materials Science and Engineering Tianjin UniversityTianjin 300072China Beijing Frontier Research Center for Biological Structures Tsinghua UniversityBeijing 100084China Ministry of Education Key Laboratory of Protein Sciences Beijing Advanced Innovation Center for Structural BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China

As the earliest commercial cathode material for lithium-ion batteries,lithium cobalt oxide(LiCoO_(2)) shows various advantages,including high theoretical capacity,excellent rate capability,compressed electrode density,*** now,it still plays an important role in the lithium-ion battery *** to these advantages,further increasing the charging cutoff voltage of LiCoO_(2)to guarantee higher energy density is an irresistible development trend of LiCoO_(2)cathode materials in the ***,using high charging cutoff voltage may induce a lot of negative effects,especially the rapid decay of cycle *** are mainly caused by rapid destruction of crystal structure and aggravation of interface side reaction at high voltage during the ***,how to maintain a stable crystal structure of LiCoO_(2)to ensure the excellent long cycle performance at high voltage is a hot research issue in the further application of LiCoO_(2).In this review,we summarized the failure causes and extensive solutions of LiCoO_(2)at high voltage and promoted some new modification ***,the development trend of solving the failure problem of high-voltage LiCoO_(2)in the future such as defect engineering and high-temperature shock technique is also discussed.

关键词： Lithium-ion battery Lithium cobalt oxide(LiCoO_(2))cathode High voltage Cycle performance Crystal structure

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Survey of Tetracyclines, Sulfonamides, Sulfamethazine, and Quinolones in UHT Milk in China Market

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Journal of Integrative Agriculture 2013年第7期12卷 1300-1305页

作者： HAN Rong-wei ZHENG nan WANG jia-qi ZHEN Yun-peng LI Song-li YU Qun-li College of Food Science and Engineering Gansu Agricultural University State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences College of Food Science and Engineering Qingdao Agricultural University

This study surveyed 180 samples of ultra high temperature （UHT） milk of four top Chinese dairy brands collected in the 25 cities in China in June 2011, and assessed their contamination with antibiotics, using the ELISA method. The percentages of tetracyclines, sulfonamides, sulfamethazine, and quinolones detected in the samples were 0, 16.7, 40.6, and 100%, respectively. The maximum concentrations of the tetracyclines, sulfonamides, sulfamethazine and quinolones in UHT milk samples were 〈1.5, 26.2, 22.6, and 58.8 μg kg-1, respectively. None of the samples exceeded the maximum residue levels （MRLs） for these four veterinary drugs, according to the regulations set by China, the European Union （EU） and the Codex Alimentarius Commission （CAC）.

关键词： survey tetracyclines sulfonamides sulfamethazine quinolones UHT milk China market

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A fluidized-bed model for NiMgW-catalyzed CO2 methanation

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Particuology 2020年第2期18卷 55-64页

作者： Chunmiao jia Yihu Dai Yanhui Yang jia wei Chew School of Chemical and Biomedical Engineering Nanyang Technological UniversitySingapore 637459Singapore Institute of Advanced Synthesis Nanjing Tech UniversityNanjing 211800Jiangsu ProvinceChina Singapore Membrane Technology Center Nanyang Technological UniversitySingapore 637141Singapore

The reduction of carbon dioxide to methane by hydrogen("CO2 methanation")using renewable energy is a promising process for recycling *** catalysts and better reactors are both required for the practical application of CO2 *** study examines how the operating parameters affect CO2 methanation in a highly efficient fluidized-bed *** first measured the kinetics of the CO2 methanation reaction using an NiMgW catalyst,which has been reported to exhibit superior catalytic *** then developed a fluidized-bed reactor model based on an earlier model for CO2 *** fluidized bed model indicated that the NiMgW was indeed superior to two other previously studied catalysts in terms of faster conversion of reactants and higher concentrations of product CH4 throughout the *** overall rate of production of CH4 increased with temperature and H2/CO2 ratio and decreased as the inlet reactant flow rate,catalyst particle diameter,and catalyst particle sphericity increased.

关键词： Fluidized-bed reactor model CO2 methanation NiMgW catalyst Reaction kinetics Sensitivity analysis

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The properties of Sn-Zn-Al-La fusible alloy for mitigation devices of solid propellant rocket motors

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Defence Technology（防务技术） 2022年第9期18卷 1688-1696页

作者： Zi-ting wei nan Li jian-xin Nie jia-hao Liang Xue-yong Guo Shi Yan Tao Zhang Qing-jie jiao State Key Laboratory of Explosion Science and Technology Beijing Institute of TechnologyBeijing100081China

The Al and La elements are added to the Sn9Zn alloy to obtain the fusible alloy for the mitigation devices of solid propellant rocket motors. Differential scanning calorimetry(DSC), metallographic analysis,scanning electron microscopy(SEM), energy dispersive spectroscopy(EDS), tensile testing and fracture analysis were used to study the effect of Al and La elements on the microstructure, melting characteristics, and mechanical properties of the Sn9Zn alloy. Whether the fusible diaphragm can effectively relieve pressure was investigated by the hydrostatic pressure at high-temperature test. Experimental results show that the melting point of the Sn9Zn-0.8Al0·2La and Sn9Zn-3Al0·2La fusible alloys can meet the predetermined working temperature of ventilation. The mechanical properties of those are more than 35% higher than that of the Sn9Zn alloy at-50°C-70°C, and the mechanical strength is reduced by 80% at 175°C. It is proven by the hydrostatic pressure at high-temperature test that the fusible diaphragm can relieve pressure effectively and can be used for the design of the mitigation devices of solid propellant rocket motors.

关键词： Mitigation devices Solid propellant rocket motors Sn9Zn Al element La element Hydrostatic pressure at high-temperature test

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Linking circular intronic RNA degradation and function in transcription by RNase H1

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Science China(Life Sciences) 2021年第11期64卷 1795-1809页

作者： Xiang Li jia-Lin Zhang Yun-Ni Lei Xiao-Qi Liu wei Xue Yang Zhang Fan nan Xiang Gao Jun Zhang jia wei Li Yang Ling-Ling Chen School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China CAS Key Laboratory of Computational Biology Shanghai Institute of Nutrition and HealthShanghai Institutes for Biological SciencesUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Section on Integrative Physiology and Metabolism Joslin Diabetes CenterHarvard Medical SchoolBostonMA 02115USA

Circular intronic RNAs(ci RNAs) escaping from DBR1 debranching of intron lariats are co-transcriptionally produced from prem RNA splicing, but their turnover and mechanism of action have remained elusive. We report that RNase H1 degrades a subgroup of ci RNAs in human cells. Many ci RNAs contain high GC% and tend to form DNA:RNA hybrids(R-loops) for RNase H1 cleavage, a process that appears to promote Pol II transcriptional elongation at ci RNA-producing loci. One ci RNA, ciankrd52, shows a stronger ability of R-loop formation than that of its cognate pre-m RNA by maintaining a locally open RNA structure in vitro. This allows the release of pre-m RNA from R-loops by ci-ankrd52 replacement and subsequent ci RNA removal via RNase H1 for efficient transcriptional elongation. We propose that such an R-loop dependent ci RNA degradation likely represents a mechanism that on one hand limits ci RNA accumulation by recruiting RNase H1 and on the other hand resolves Rloops for transcriptional elongation at some GC-rich ci RNA-producing loci.

关键词： circular intronic RNA ciRNA ci-ankrd52 ciRNA structure DNA:RNA hybrid R-loop RNase H1 transcriptional elongation

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Rational drug design, synthesis, and biological evaluation of novel chiral tetrahydronaphthalene-fused spirooxindole as MDM2-CDK4 dual inhibitor against glioblastoma

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Acta Pharmaceutica Sinica B 2020年第8期10卷 1492-1510页

作者： Biao Wang Fu Peng wei Huang Jin Zhou nan Zhang jia Sheng Phensinee Haruehanroengra Gu He Bo Han Hospital of Chengdu University of Traditional Chinese Medicine State Key Laboratory of Southwestern Chinese Medicine ResourcesSchool of PharmacyChengdu University of Traditional Chinese MedicineChengdu 611137China State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan University and Collaborative Innovation Center for BiotherapyChengdu 610041China Department of Chemistry and the RNA Institute University at AlbanyState University of New YorkAlbanyNY 12222USA

Simultaneous inhibition of MDM2 and CDK4 may be an effective treatment against glioblastoma. A collection of chiral spirocyclic tetrahydronaphthalene(THN)-oxindole hybrids for this purpose have been developed. Appropriate stereochemistry in THN-fused spirooxindole compounds is key to their inhibitory activity: selectivity differed by over 40-fold between the least and most potent stereoisomers in time-resolved FRET and KINOMEscan~? in vitro assays. Studies in glioblastoma cell lines showed that the most active compound ent-4g induced apoptosis and cell cycle arrest by interfering with MDM2-P53 interaction and CDK4 activation. Cells treated with ent-4g showed up-regulation of proteins involved in P53 and cell cycle pathways. The compound showed good anti-tumor efficacy against glioblastoma xenografts in mice. These results suggested that rational design, asymmetric synthesis and biological evaluation of novel tetrahydronaphthalene fused spirooxindoles could generate promising MDM2-CDK4 dual inhibitors in glioblastoma therapy.

关键词： MDM2 glioblastoma CDK4

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Theoretical uncertainties of(d,^(3)He)and(^(3)He,d)reactions owing to the uncertainties of optical model potentials

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Nuclear Science and Techniques 2023年第6期34卷 147-153页

作者： wei-jia Kong Dan-Yang Pang School of Physics Beihang UniversityBeijing100191China School of Physics and Beijing Key Laboratory of Advanced Nuclear Materials and Physics Beihang UniversityBeijing100191China

The theoretical uncertainties of single proton transfer cross sections of the(^(3)He,d)and(d,^(3)He)reactions,owing to the uncertainties of the entrance-and exit-channel optical model potentials,are examined with the^(30)Si(^(3)He,d)^(31)P,^(13)B(d,^(3)He)^(12)Be,and^(34)S(^(3)He,d)^(35)Cl reactions at incident energies of 25,46,and 25 MeV,respectively,within the framework of the distorted wave Born *** differential cross sections at the first peaks in the angular distributions of these reactions are found to have uncertainties of approximately 5%,owing to the uncertainties in the optical model potentials from 20,000 calculations of randomly sampled *** amount of uncertainty is found to be nearly independent of the angular momentum transfer and the target masses within the studied range of incident *** in the single proton spectroscopic factors obtained by matching the theoretical and experimental cross sections at different scattering angles are also discussed.

关键词： Proton transfer reactions Optical model potentials Spectroscopic factors

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A New Feistel-Type White-Box Encryption Scheme

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Journal of Computer Science & Technology 2017年第2期32卷 386-395页

作者： Ting-Ting Lin Xue-jia Lai wei-jia Xue Yin jia Cryptography and Information Security Laboratory Department of Computer Science Shanghai Jiao Tong University Shanghai 200240 China Irdeto Canada Ottawa Ontario K2K3G5 Canada

The white-box attack is a new attack context in which it is assumed that cryptographic software is implemented on an un-trusted platform and all the implementation details are controlled by the attackers. So far, almost all white-box solutions have been broken. In this study, we propose a white-box encryption scheme that is not a variant of obfuscating existing ciphers but a completely new solution. The new scheme is based on the unbalanced Feistel network as well as the ASASASA （where ＂A＂ means affine, and ＂S＂ means substitution） structure. It has an optional input block size and is suitable for saving space compared with other solutions because the space requirement grows slowly （linearly） with the growth of block size. Moreover, our scheme not only has huge white-box diversity and white-box ambiguity but also has a particular construction to bypass public white-box cryptanalysis techniques, including attacks aimed at white-box variants of existing ciphers and attacks specific to the ASASASA structure. More precisely, we present a definition of white-box security with regard to equivalent key, and prove that our scheme satisfies such security requirement.

关键词： white-box equivalent key Feistel network cryptography ASASASA

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