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Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells

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Journal of Pharmaceutical Analysis 2022年第5期12卷 801-807页

作者： Xin-Yuan Hu jia-jing li Zi-Wei Yang Jun Zhang Huai-Song Wang Department of Pharmaceutical Analysis China Pharmaceutical UniversityNanjing210009China College of Food Science and Bioengineering Tianjin Agricultural UniversityTianjin300392China

The catalytic generation of H_(2) in living cells provides a method for antioxidant *** this study,an[FeFe]-hydrogenase mimic[Ru+Fe_(2)S_(2)@F127(80)]was synthesized by self-assembling polymeric pluronic F-127,catalytic[Fe_(2)S_(2)]sites,and photosensitizer Ru(bpy)_(3)^(2+).Under blue light irradiation,hydrated protons were photochemically reduced to H2,which increased the local pH in living cells(HeLa cells).The generated H2 was subsequently used as an antioxidant to decrease reactive oxygen species(ROS)levels in living cells(HEK 293T,HepG2,MCF-7,and HeLa cells).Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times,relative to that of other cells(HepG2,MCF-7,and HeLa cells).Intracellular ROS and pH levels were then monitored using fluorescent cell *** study showed that cell imaging can be used to evaluate the ability of Ru t Fe2S2@F127 to eliminate oxidative stress and prevent ROS-related diseases.

关键词： Cell imaging [FeFe]-hydrogenase Reactive oxygen species Photocatalytic system

Morphological and biochemical characteristics associated with autophagy in gastrointestinal diseases

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World Journal of Gastroenterology 2024年第11期30卷 1524-1532页

作者： Yi-Fan Chang jia-jing li Tao liu Chong-Qing Wei li-Wei Ma Vladimir N Nikolenko Wei-Long Chang Department of Gastrointestinal Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Department of Pathology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Department of Clinical Laboratory The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Department of Human Anatomy and Histology I.M.Sechenov First Moscow State Medical University(Sechenov University)Moscow 119991Russia

Autophagy is a cellular catabolic process characterized by the formation of double-membrane *** electron microscopy is the most rigorous method to clearly visualize autophagic engulfment and degradation.A large number of studies have shown that autophagy is closely related to the digestion,secretion,and regeneration of gastrointestinal(GI)***,the role of autophagy in GI diseases remains *** article focuses on the morphological and biochemical characteristics of autophagy in GI diseases,in order to provide new ideas for their diagnosis and treatment.

关键词： Autophagy Morphological study Biochemical characteristics Subcellular structure Transmission electron microscopy Gastrointestinal diseases

Synchronous occurrence of extramedullary plasmacytoma and squamous cell carcinoma in situ in the larynx: a case report

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Chinese Journal of Cancer 2010年第12期29卷 1029-1034页

作者： Xiao-li Zhang Dai-Qiang li jing-jia li Shi-Sheng li Xin-Ming Yang Department of Otolaryngology-Head and Neck Surgery the Second Xiangya Hospital Central South University Changsha Hunan 410011 P. R. China Department of Pathology the Second Xiangya Hospital Central South University Changsha Hunan 410011 P. R. China

Extramedullary plasmacytoma of the larynx is rare, especially when coexisted with squamous cell carcinoma in situ. We report a 56-year-old woman with hoarseness for 6 months and dysphonia for a week. Fiberoptic laryngoscopic examination showed a red, smooth-surface swelling in the submucous region of the left ventricle and ventricular band of the larynx. The patient underwent vertical laryngectomy and modified left neck dissection. Postoperative pathologic examination revealed coexisting plasmacytoma and carcinoma in situ. Bone marrow biopsy and systemic radiogram showed no positive findings. The hepatic and renal functions were normal. Monoclonal immunoglobulin light chain of type kappa was detected in urine. Hence, a laryngeal extramedullary plasmacytoma with carcinoma in situ was diagnosed. No recurrence or progression was observed during a 2-year follow-up. Here, we discussed the risk factors, diagnosis, and therapy for this rare disease.

关键词：鳞状细胞癌原位病例报告浆细胞同步病理检查免疫球蛋白危险因素

The development of laminin-alginate microspheres encapsulated with Ginsenoside Rg1 and ADSCs for breast reconstruction after lumpectomy

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Bioactive Materials 2021年第6期6卷 1699-1710页

作者： I-Hsuan Yang Yo-Shen Chen jia-jing li Ya-Jyun liang Tzu-Chieh lin Subhaini Jakfar Minal Thacker Shinn-Chih Wu Feng-Huei lin Department of Biomedical Engineering College of Medicine and College of EngineeringNational Taiwan UniversityNo.49Fanglan RdTaipei10672China Division of Plastic Surgery Department of SurgeryFar Eastern Memorial HospitalNo.21Sec.2Nanya S.Rd.New Taipei City220China Department of Animal Science and Technology National Taiwan UniversityNo.1Section 4Roosevelt RoadTaipei City10617China Institute of Biomedical Engineering and Nanomedicine National Health Research InstitutesNo.35Keyan RoadZhunanMiaoli County35053China

Many technologies have been developed for breast reconstruction after *** the technologies achieved promising success in clinical,there are still many shortages hanging over and trouble the *** engineering technology was introduced to plastic surgery that gave a light to lumpectomy patients in breast *** unexpected absorption rate,resulting from limited vascularization and low cell survival rate,is a major factor that leads to unsatisfactory results for the previous studies in our *** the study,the laminin-modified alginate synthesized by a new method of low concertation of sodium periodate would be mixed with ADSCs and Rg1 in the medium;and then sprayed into a calcium chloride(CaCl2)solution to prepare into microsphere(abbreviated as ADSC-G-LAMS)by bio-electrospray with a power syringe for the mass production and smaller bead *** developed ADSC-G-LAMS microspheres had the diameter of 232±42μ***-release of the Rg1 retained its biological ***-1,live/dead staining,and chromosome aberration assay were evaluated to confirm the safety of the *** in vivo study,ADSC-G-LAMS microspheres combined with autologous adipocytes were transplanted into the dorsum of rats by subcutaneous *** efficacy was investigated by H&E and immunofluorescence *** results showed that the bioactive ADSC-G-LAMS microspheres could integrate well into the host adipose tissue with an adequate rate of angiogenesis by constantly releasing Rg1 to enhance the ADSC or adipocyte survival rate to join tissue growth and repair with adipogenesis for breast reconstruction after lumpectomy.

关键词： Laminin-alginate microspheres Adipose-derived stem cells Ginsenoside Rg1 Bio-electrospray Tissue engineering

基于混合优化方法的UniTire轮胎模型参数辨识

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吉林大学学报（工学版） 2017年第1期47卷 15-20页

作者：张家旭李静吉林大学汽车仿真与控制国家重点实验室长春130022 中国第一汽车集团技术中心长春130011

提出一种基于粒子群优化(PSO)算法和修正的Gauss-Newton算法的混合优化方法,对纯纵滑和纯侧偏工况下UniTire轮胎模型的特征参数进行辨识。为充分发挥两个算法的优点,首先,利用粒子群优化算法的全局区域的搜索优势辨识出UniTire轮胎模型... 详细信息

提出一种基于粒子群优化(PSO)算法和修正的Gauss-Newton算法的混合优化方法,对纯纵滑和纯侧偏工况下UniTire轮胎模型的特征参数进行辨识。为充分发挥两个算法的优点,首先,利用粒子群优化算法的全局区域的搜索优势辨识出UniTire轮胎模型的特征参数近似解,然后,利用修正的Gauss-Newton算法局部搜索优势在近似解邻近区域获得UniTire轮胎模型的特征参数的最优解。最后,对辨识结果进行残差分析,结果表明:用辨识数据参数化的UniTire轮胎模型具有较高的精度,可满足构建车辆底盘电控系统硬件在环仿真测试环境的需求。

关键词：车辆工程粒子群优化算法 Gauss-Newton算法 UniTire轮胎模型参数辨识

Landscape Change and its effect on the environment of Daqing City

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Progress in Natural Science:Materials International 2005年第7期15卷 641-649页

作者： ZANG Shuying, ZHANG jing and jia li ( College of life and Environment Science, Harbin Normal University, Harbin 150080, China College of Resources Environment & Tourism, Capital Normal University, Beijing 100037, China College of Economics and Management, Northeast Agricultural University, Harbin 150030, China) College of life and Environment Science Harbin Normal University Harbin 150080 China. College of Resources Environment & Tourism Capital Normal University Beijing 100037 China. College of Economics and Management Northeast Agricultural University Harbin 150030 China

In this paper, we will study the temporal-spatial landscape change from 1978 to 2001 in Daqing City, based on the mul- ti spectral scanner (MSS) and thematic mapper (TM) images of remote sensing (RS) of 1978, 1988, 1992, 1996 and 2001. In order to explain the effects of the component change on the whole landscape, we make a statistic analysis about the transfer probability matrix of the component types of the landscape by using the ARCGIS platform through the overlay analysis of different landscape components. Based on the transfer probability matrix, we calculate the in-transfer ratio, the out-transfer ratio and the contributor ratio of the special transfer process of the main landscape components. The results illustrate that the cultivated-land, grassland, swamp, and alkaline land are the main contributors resulting in the landscape change: the out-transfer ratios of grassland and swamp are much higher than the in-transfer ones; the in-transfer ratio of alkaline land is much higher than the out-transfer one; the in-transfer ratio of the land-lot is always higher than its out-transfer with the highest rate per year, although there is a small difference among the in-transfers and the out-transfers per year. The main landscape changing processes are the salination of cultivated-land, salination or desertification of grassland, salination and degeneration of swamp. Results in this paper can provide scientific basis for investigating the landscape change mechanism of resources-based city.

关键词： landscape change environmental effect transfer probability resources-based city.

Type I methionine aminopeptidase from Saccharomyces cerevisiae is a potential target for antifungal drug screening

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Acta Pharmacologica Sinica 2004年第7期25卷 61-68页

作者： ling-ling CHEN, jia li, jing-ya li, Qun-li LUO, Wei-feng MAO, Qiang SHEN, Fa-jun NAN3, Qi-zhuang YE2,3 NationalCenter for Drug Screening, Instituteof Materia Medica, Shanghai Institutes for BiologicalSciences, Chinese AcademyofSciences, Shanghai 201203, China 2 The High Throughput Screening Laboratory, University of Kansas, Lawrence, Kansas 66047, USA National Center for Drug Screening Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

AIM: To screen antifungal drug candidates using in vitro and in vivo assays based on type I methionine aminopep- tidase from Saccharomyces cerevisiae (ScMetAP1). METHODS: A colorimetric assay suitable for high throughput screening (HTS) using recombinant ScMetAP1 protein expressed in Escherichia coli was established for antifungal lead discovery. A series of pyridine-2-carboxylic acid derivatives were characterized and a chemical library of 12 800 pure organic compounds was screened with the in vitro ScMetAP1 assay. Active compounds from the in vitro assay were further evaluated by a growth inhibition assay on yeast strain with deletion of ScMetAP1 gene map1 in comparison with the wild-type yeast strain and the yeast strain with deletion of type II enzyme (ScMetAP2) gene map2. RESULTS: Active ScMetAP1 inhibitors were identified from HTS. Some of the pyridine-2-carboxylic acid derivatives (compound 2 and 3) had selective inhibition of the growth of map2 deletion yeast and weak inhibition on wild-type yeast growth, while no inhibition on map1 deletion yeast. CONCLUSION: ScMetAP1 is a novel potential target for developing antifungal drugs. The in vitro and in vivo ScMetAP1 assays can serve as tools in discovering antifungal drug candidates.

关键词： methionine aminopeptidase high-throughput screening antifungal agent

土地利用/覆被变化对区域径流的影响——以秦皇岛地区为例

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地理与地理信息科学 2017年第2期33卷 108-114,F0003页

作者：贾静石晓丽河北师范大学资源与环境科学学院河北石家庄050024 河北省环境演变与生态建设重点实验室河北石家庄050024

土地利用方式的变化是影响流域径流的重要因素。为定量研究土地利用/覆被变化对径流的影响,该文结合SWAT(Soil and Water Assessment Tool)分布式水文模型,分析了秦皇岛地区2000年、2005年、2010年和2014年不同土地利用/覆被变化对径流... 详细信息

土地利用方式的变化是影响流域径流的重要因素。为定量研究土地利用/覆被变化对径流的影响,该文结合SWAT(Soil and Water Assessment Tool)分布式水文模型,分析了秦皇岛地区2000年、2005年、2010年和2014年不同土地利用/覆被变化对径流的影响,并在子流域尺度上分析径流的空间变化。研究结果表明:1)SWAT模型能够较好地模拟研究区流域的月径流过程。2000-2005年研究区耕地急剧增加,林地和裸地减少,导致多年平均径流量增加4.79%;2005-2010年林地明显增加,耕地和裸地减少,导致多年平均径流量减少3.32%;2010-2014年城镇建设用地增加明显,耕地和林地减少,导致多年平均径流量增加2.62%。2)耕地和城镇建设用地的增加在不同程度上会增加径流量,而林地具有涵养水源、减少径流量的作用。3)研究区年均地表径流变化空间差异较显著,整体呈增加趋势。研究结果可为合理规划土地利用格局、优化配置水资源提供参考依据。

关键词：土地利用/覆被变化 SWAT模型径流秦皇岛

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寡糖诱导向日葵抗锈病的信号转导途径

中国油料作物学报 2012年第5期34卷 523-527页

作者：景岚李凌欣裴旭景佳丽内蒙古农业大学农学院内蒙古呼和浩特010019

为了解寡糖诱导向日葵抗锈病过程中的信号转导途径,利用寡糖、水杨酸(SA)、茉莉酸(JA)预先喷雾处理向日葵叶片,1d后接种锈菌,通过对植株体内脂氧合酶(LOX)活性及SA含量的测定,并通过RT-PCR对SA、JA信号途径的标记基因PR5和PDF1.2等表达... 详细信息

为了解寡糖诱导向日葵抗锈病过程中的信号转导途径,利用寡糖、水杨酸(SA)、茉莉酸(JA)预先喷雾处理向日葵叶片,1d后接种锈菌,通过对植株体内脂氧合酶(LOX)活性及SA含量的测定,并通过RT-PCR对SA、JA信号途径的标记基因PR5和PDF1.2等表达水平进行测定,初步确定诱导抗性表达的信号转导途径。结果表明:经寡糖诱导后,向日葵叶片内LOX活性降低,SA含量上升,且诱导水杨酸途径标记基因PR5表达量增加。结果说明寡糖诱导向日葵抵抗锈病主要与SA介导的信号途径有关。

关键词：向日葵锈病信号转导途径寡糖脂氧合酶水杨酸茉莉酸