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Human umbilical cord-derived mesenchymal stem cells differentiate into epidermal-like cells using a novel co-culture technique

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Human umbilical cord-derived mesenchymal stem cells differen...

A transcription factor STOP1-centered pathway coordinates ammonium and phosphate acquisition in Arabidopsis

第七届全国医学生物化学与分子生物学和第四届全国临床应用生物化学与分子生物学联合学术研讨会暨医学生化分会会员代表大会

作者： Li Dong-jie Chai jia-ke Han Yan-fu Sun tian-jun Burn Institute the First Affiliated Hospital of PLA General Hospital

Human umbilical cord-derived mesenchymal stem cells(hUCMSCs) isolated from human umbilical Wharton’s Jelly are a population of primitive and pluripotent *** different specific conditions,they can differentiate into different cells,such as adipocytes,osteoblasts,chondrocytes, neurocytes,endothelial cells ***,few reports on differentiating hUCMSCs into epidermal cells in vitro were *** are several methods of MSCs differentiation for choice,such as the specific induction medium,direct or indirect *** this study,We have developed a microporous membrane-based indirect co-culture system for hUCMSCs differentiation,in which epidermal stem cells were seeded on the bottom of microporous membrane and the culture flask of the 6-well dish,hUCMSCs were seeded on the top of microporous *** system allows real-time conditioning of the culture medium with human epidermal cells while still maintaining the complete separation of the two cell types. HUCMSCs in this indirect co-culture system expressed the early markers of epidermal cell lineage,P63, cytokeratinl9(CK19),andβ*** morphologies were changed from spindle-like fibroblastic appearances to oblate or irregular shapes under phase contrast *** these data not only highlight the possibility of in vitro control of hUCMSCs into epidermal lineage,but also suggest a novel indirect co-culture model for MSCs differentiation.

关键词： human umbilical cord-derived mesenchymal stem cells differentiation epidermal-like cells microporous membrane indirect co-culture system

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Molecular Plant 2021年第9期14卷 1554-1568页

作者： Wen Hao tian jia Yuan Ye Meng Qi Cui Jun Bo Chang Yu Liu GuiXin Li Yun Rong Wu Ji Ming Xu Nicholas P.Harberd Chuan Zao Mao Chong Wei Jin Zhong Jie Ding Shao jian Zheng State Key Laboratory of Plant Physiology and Biochemistry College of Life ScienceZhejiang UniversityHangzhou 310058China College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310038China College of Agronomy and Biotechnology Zhejiang UniversityHangzhou 310038China Department of Plant Sciences University of OxfordOxford OX13RBUK Guangdong Laboratory for Lingnan Modern Agriculture College of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 5100642China

Phosphorus(P)is an indispensable macronutrient required for plant growth and *** phosphate(Pi)reserves are finite,and a better understanding of Pi utilization by crops is therefore vital for worldwide food *** has long been known to enhance Pi acquisition efficiency in agriculture;however,the molecular mechanisms coordinating Pi nutrition and ammonium remains ***,we reveal that ammonium is a novel initiator that stimulates the accumulation of a key regulatory protein,STOP1,in the nuclei of Arabidopsis root cells under Pi *** show that Pi deficiency promotes ammonium uptake mediated by AMT1 transporters and causes rapid acidification of the root *** acidification-triggered STOP1 accumulation activates the excretion of organic acids,which help to solubilize Pi from insoluble iron or calcium *** uptake by AMT1 transporters is downregulated by a CIPK23 protein kinase whose expression is directly modulated by STOP1 when ammonium reaches toxic *** together,we have identified a STOP1-centered regulatory network that links external ammonium with efficient Pi acquisition from insoluble phosphate *** findings provide a framework for developing possible strategies to improve crop production by enhancing the utilization of non-bioavailable nutrients in soil.

关键词： Pi deficiency response ammonium STOP1 organic acid exudation CIPK23

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AFM常见针尖与试样面间力的计量研究

计量学报 2005年第3期26卷 242-244,288页

作者：田文超贾建援西安电子科技大学机电工程学院陕西西安710071

首先建立了原子力显微镜(AFM)针尖与试样面力学计量的物理模型,介绍了研究该问题的方法。根据Hamaker理论,利用微观连续介质法建立了AFM常见的圆锥型、四棱锥型和抛物面型针尖与试样面间的包含斥力的力学模型。经计算得到仿真结果,仿真... 详细信息

首先建立了原子力显微镜(AFM)针尖与试样面力学计量的物理模型,介绍了研究该问题的方法。根据Hamaker理论,利用微观连续介质法建立了AFM常见的圆锥型、四棱锥型和抛物面型针尖与试样面间的包含斥力的力学模型。经计算得到仿真结果,仿真结果与实验结果一致。

关键词：计量学原子力显微镜微观连续介质法 Hamaker假设

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疏松砂岩地层压裂充填支撑剂粒径优选

中国石油大学学报（自然科学版） 2012年第1期36卷 94-100页

作者：郭天魁张士诚王雷贺甲元中国石油大学石油工程学院北京102249 中石化石油勘探开发研究院北京100083

在疏松软地层中实施压裂充填工艺,出砂和支撑剂的嵌入是造成裂缝失效的主要因素,而两者都与支撑剂的粒径有密切的关系。针对金县油田高渗储层,设计模拟裂缝壁面的嵌入与砂侵和裂缝端部的砂侵试验。在不同压力下,对两种不同粒度组成的模... 详细信息

在疏松软地层中实施压裂充填工艺,出砂和支撑剂的嵌入是造成裂缝失效的主要因素,而两者都与支撑剂的粒径有密切的关系。针对金县油田高渗储层,设计模拟裂缝壁面的嵌入与砂侵和裂缝端部的砂侵试验。在不同压力下,对两种不同粒度组成的模拟地层砂进行测试,获得支撑剂在软地层中的嵌入程度、不同粒径支撑剂及其组合下的出砂量、出砂粒径以及砂侵后的导流能力。结果表明:在软地层中,支撑剂的嵌入程度随支撑剂粒径的增加而增大,随地层砂粒度中值的增大而减小;压裂充填防砂支撑剂粒径应优选为5～9倍的地层砂粒度中值;支撑剂粒径是地层砂粒度中值的6～9倍时,可形成内部砂桥;适合于压裂充填防砂的支撑剂粒径组合方案是粒径为地层砂粒度中值6～9倍的支撑剂与小于6倍的支撑剂体积比为3∶1。

关键词：砂岩压裂充填支撑剂嵌入导流能力砂侵

Time representation of mitochondrial morphology and function after acute spinal cord injury

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Neural Regeneration Research 2016年第1期11卷 137-143页

作者： Zhi-qiang jia Gang Li Zhen-yu Zhang Hao-tian Li Ji-quan Wang Zhong-kai Fan Gang Lv Department of Orthopedics First Affiliated Hospital of Liaoning Medical University

Changes in mitochondrial morphology and function play an important role in secondary damage after acute spinal cord injury. We recorded the time representation of mitochondrial morphology and function in rats with acute spinal cord injury. Results showed that mitochondria had an irregular shape, and increased in size. Mitochondrial cristae were disordered and mitochondrial membrane rupture was visible at 2–24 hours after injury. Fusion protein mitofusin 1 expression gradually increased, peaked at 8 hours after injury, and then decreased to its lowest level at 24 hours. Expression of dynamin-related protein 1, amitochondrial fission protein, showed the opposite kinetics. At 2–24 hours after acute spinal cord injury, malondialdehyde content, cytochrome c levels and caspase-3 expression were increased, but glutathione content, adenosine triphosphate content, Na＋-K＋-ATPase activity and mitochondrial membrane potential were gradually reduced. Furthermore, mitochondrial morphology altered during the acute stage of spinal cord injury. Fusion was important within the first 8 hours, but fission played a key role at 24 hours. Oxidative stress was inhibited, biological productivity was diminished, and mitochondrial membrane potential and permeability were reduced in the acute stage of injury. In summary, mitochondrial apoptosis is activated when the time of spinal cord injury is prolonged.

关键词： nerve regeneration spinal cord injury mitochondria fusion fission oxidative damage bioenergy mitochondrial permeability cytochrome c Caspase-3 apoptosis NSFC neural regeneration

Stacked Denoising Autoencoder based Fault Diagnosis for Rotating Motor

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Stacked Denoising Autoencoder based Fault Diagnosis for Rota...

第37届中国控制会议

作者： Haichuan Tang Kunting Zhang Dingfei Guo Lihao jia Hong Qiao Yin tian Cloud Computing Center Chinese Academy of Sciences Beijing Key Laboratory of Research and Application for Robotic Intelligence of “Hand-Eye-Brain” Interaction Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences University of Chinese Academy of Sciences

Fault diagnosis is vital for normal operation of the rotating *** effective and reliable deep learning method known as stacked denoising autoencoder（SDAE） is investigated in this paper,which can extract the features from the pending signals with *** adaptive networks are designed to extract features automatically from time domain data and frequency domain data of motor vibration signal,***,the network parameters of the SDAE are trained to reconstruct the signal features,and clustering results are ***,a classification layer is added to the top layer of the SDAE network for the fault *** is shown that,the diagnosis accuracy with input of vibratory frequency signal is higher than that of time domain *** features extracted by SDAE can represent complex mapping relationships between signal and various running status,and the accuracy is improved comparing with traditional fault diagnosis methods.

关键词： Rotating motor Fault diagnosis SDAE Deep learning

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近视研究中动物模型的选择

中华眼科杂志 2005年第6期41卷 486-487页

作者：周翔天瞿佳温州医学院眼视光学院 325003

近视动物模型是近视研究的重要工具,但在动物选择、处理方法等问题上存在很多差异和争议。近视动物模型的选择不仅可决定实验研究成功与否,还能决定实验结果是否准确和正确。因此,有必要围绕近视动物模型中几个有争议的问题展开讨论,同... 详细信息

近视动物模型是近视研究的重要工具,但在动物选择、处理方法等问题上存在很多差异和争议。近视动物模型的选择不仅可决定实验研究成功与否,还能决定实验结果是否准确和正确。因此,有必要围绕近视动物模型中几个有争议的问题展开讨论,同时提出一些意见,仅供参考。

关键词：动物模型近视动物选择处理方法实验研究

Comparison of traditional vascular reconstruction with covered stent in the treatment of subclavian artery injury

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Chinese Journal of Traumatology 2020年第1期23卷 25-28页

作者： Xuan tian jian-Long Liu Wei jia Peng jiang Zhi-Yuan Cheng Yun-Xin Zhang Jin-Yong Li Chen-Yang tian Department of Vascular Surgery Beijing Jishuitan HospitalBeijing 100035China

Purpose:To explore the significance of traditional vascular reconstruction and covered stent for limb salvage after subclavian artery ***:Patients with subclavian artery injury admitted to Beijing Jishuitan Hospital from January 2010 to December 2018 were retrospectively *** the injuries have been confirmed by intraoperative exploration,computed tomography angiography or digital subtraction *** or partial amputation injuries were *** artery defect or partial intimal damage was treated by interventional implantation,while other patients received open surgeries,including direct suture of small defect less than 2 cm and transplantation with autologous vein or artificial blood when the defect was more than 2 *** were divided into open surgery group and stent implantation group based on the treatment they *** were followed up at 2 weeks(first stage)and 6 months(second stage)after operation to investigate limb ***'s t-test was used to compare the general data between two groups and Chi-square test to analyze the rate of limb ***:Altogether 50 cases of subclavian artery injury were treated,including 36 cases of open surgery and 14 cases of stent *** of nerve injury was observed in 27 cases(75.0%)in open surgery group and 12 cases(85.7%)in stent implantation *** developed in 3 cases with open surgery and 1 case with stent *** the rate of successful limb salvage was respectively 91.7%(33/36)and 92.9%(13/14),revealing no significant difference(p>0.05).Conclusion:Rapid reconstruction of blood circulation is crucial following subclavian artery injury,no matter what kinds of treatment strategies have been *** stent implantation can achieve a good effect for limb salvage.

关键词： Subclavian artery injury Vascular transplantation Covered stent

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计算表面波特征方程的一种新的数值方法

电波科学学报 2006年第6期21卷 859-862,884页

作者：田雨波钱鉴生佳根江苏科技大学电子信息学院南京大学电子科学与工程系江苏南京210093 南京大学电子科学与工程系

推导了同轴线馈电的微带天线表面波电磁场表达式及其特征方程。结合遗传算法与参数跟踪策略形成一种新的算法,并基于该算法对表面波特征方程进行了精确数值求解,求解结果与本问题的已有结论吻合的很好。该方法具有简单、通用、求解精度... 详细信息

推导了同轴线馈电的微带天线表面波电磁场表达式及其特征方程。结合遗传算法与参数跟踪策略形成一种新的算法,并基于该算法对表面波特征方程进行了精确数值求解,求解结果与本问题的已有结论吻合的很好。该方法具有简单、通用、求解精度高等优点。

关键词：微带天线表面波遗传算法