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Mitochondrial genome sequencing with short overlapping amplicons on MiSeq FGx system

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Forensic Sciences Research 2022年第2期7卷 142-153页

作者： Yang Xin Rulin jia Suhua zh.ng Fei Guo Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceMinistry of JusticeShanghaiChina Forensic Science College Criminal Investigation Police University of ChinaShenyangChina

With the development and maturation of massively parallel sequencing(MPS)technology,the mitochondrial genome(mitogenome)sequencing is increasingly applied in the forensic *** this study,we employed the strategy of short overlapping amplicons for the whole mitogenome,library preparation with tagmentation using the Nextera®XT DNA Library Preparation Kit,sequencing on the MiSeq FGxTM Forensic Genomics System and analyzing data using the mitochondrial(mtDNA)MSR Plug-in and the mtDNA Variant Analyzer.A total of 27 libraries and 56 libraries were sequenced in a run using MiSeq Reagent Kit v2 and v3,*** showed more than 1800×of averaged depth of coverage(DoC)at each *** haplotypes of 9947 A and 2800 M were obtained at 32 ***-reactivity was observed with 1 ng primate DNA and 10 ng non-primate DNA but could be easily *** and accurate variants were obtained from at least 50 pg input DNA and from minor contributors between 19:1 and 1:19 mixed ratios with known reference *** than 86%variants were detected from≥200-bp degraded samples but its haplotype was assigned to more ancestral ***,a total of 3962 variants were observed at 613 nucleotide positions from 103 Xibe mitogenomes with 25:1 ratio of transitions to *** new transversions(C13735A and A14755C)and two tri-alleles at nps 9824 and 16092 were *** were 103 unique mitogenome haplotypes from 103 Chinese Xibe that were assigned to 79 *** D was the preponderant top-level haplogroup in Xibe followed by F,B,M,A,N,G,C,Z,Y,HV and *** match probability(RMP)and haplotype diversity(HD)of the whole mitogenome was calculated as 0.0097 and 1.0000,*** with HVS-I only,RMP decreased 33.56%,while the number of haplotypes and HD increased 15.73%and 0.49%,*** component analysis(PCA)showed that Xibe was clustered to East and Southeast *** a whole,this MPS strategy i

关键词： Forensic sciences massively parallel sequencing MiSeq FGx system mitochondrial genome Xibe degraded samples

Effect of martensitic transformation on nano/ultrafine-grained structure in 304 austenitic stainless steel

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Journal of Iron and Steel Research International 2017年第12期24卷 1231-1237页

作者： Na Gong Hui-bin Wu Gang Niu jia-ming Cao Da zh.ng Tana Instituted Engineering Technology University of Science and Technology BeijingBeiking 100083China Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China Sinohydro Renewable Energy Co. Ltd.Beijing 100083China

304 austenitic stainless steel was cold rolled in the range of 20%-80%reductions and then annealed at 700-900°C for 60 sto obtain nano/ultrafine-grained（ng/UFG）*** electron microscopy,electron backscatter diffraction and X-ray diffraction were used to characterize the resulting *** results showed that with the increase of cold reduction,the content of martensite was *** steel performed work hardening during cold-working owing to the occurrence of strain induced martensite which nucleated in single shear *** rolling broke up the lath-type martensite into dislocation-cell type martensite because of the formation of slip *** annealed at 800-960°C for 60 swere of ng/UFG structure with different percentage of nanocrystalline（60-100 nm）and ultrafine（100-500 nm）grains,submicron size（500-1000 nm）grains and micron size（〉1000 nm）*** value of the Gibbs free energy exhibited that the reversion mechanism of the reversion process was shear controlled by the annealing *** a certain annealing time during the reversion process,austenite nucleated first on dislocation-cell type martensite and the grains grew up subsequently and eventually to be micrometer/submicrometer grains,while the nucleation of austenite on lath-type martensite occurred later resulting in nanocrystalline/ultrafine *** existence of the ng/UFG structure led to a higher strength and toughness during tensile test.

关键词： 304 austenitic stainless steel Nano/ultrafine-grained structure Reversion mechanism Lath-type martensite Dislocation-cell type martensite Martensitic transformation

Chemiluminescence Determination of Molybdenum by on-Line Reduction with a Flow Injection System

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Chinese Chemical Letters 2000年第5期11卷 427-430页

作者： zhi jia zhU (Experimental Center, Hebei Normal University, Shijiazhuang 050016) Experimental Center Hebei Normal University Shijiazhuang 050016 China

The chemiluminescence (CL) reaction between lucigenin (Lu) and molybdenum (III) produced by a Jones reductor was investigated using a flow injection system. On the basis of this, a novel method for the determination of trace amount of molybdenum has been established. The emission intensity was linear with molybdenum concentration in the range of 0.1-1000 ng/mL; the detection limit was 0.02 ng/mL molybdenum; the relative standard deviation was less than 2% for the determination of 0.1 ng/mL molybdenum (n=11). The method has been applied successfully to the analysis of trace molybdenum in water and steel samples.

关键词： chemiluminescence analysis reductant flow-injection molybdenum lucigenin

Expression of liver insulin-like growth factor 1 gene and its serum level in patients with diabetes

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World Journal of Gastroenterology 2004年第2期10卷 255-259页

作者： jian-BoLi Cheng-YaWang jia-WeiChen zhen-QingFeng Hong-TaiMa DepartmentofEndocrinology FirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029JiangsuProvinceChina MolecularLaboratory FirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029JiangsuProvinceChina DepartmentofPathology NanjingMedicalUniversityNanjing210029JiangsuProvinceChina

AIM:To explore the effect of diabetic duration and blood glucose level on insulin like growth factor 1(IGF-1) gene expression and serum IGF-1 ***: Diabetes was induced into Sprague Dawley rats by alloxan and then the rats were subdivided into different groups with varying blood glucose level and diabetic *** parameters were measured as follows:IGF-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR),IGF-1 peptide and serum IGF-1 concentration by enzymelinked immunosorbent assay (ELISA).RESULTS:During early diabetic stage (week 2),in comparison with normal control group (NC), IGF-1 mRNA(1.17±0.069 vs 0.79±0.048, Png·mg^-1 vs(128.2±11.25)ng·mg^-1,Png·mg^-1 vs(74.43±5.33)ng·mg^-1, Png·mg^-1 vs (202.05±15.73)ng·mg^-1,Png·mg^-1 vs(202.05±15.73)ng·mg^-1,Png·mg^-1 vs(511.2±24.7)ng·mg^-1,Png·mg^-1 vs(511.2±24.7)ng·mg^-1, Png·mg^-1 vs(520.7±26.32)ng·mg^-1,Png·mg^-1,Pnge was significantly correlated with that in liver tissue (r=-0.99, P<0.001).Furthermore,No difference was found in the above parameters between diabetic rats with euglycemia and non-diabetic control ***: The influence of diabetic status on IGF-1 gene expression in li

关键词：胰岛素样生长因子1 基因表达血清学检查糖尿病逆转录聚合酶链反应 ELISA 动物模型

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蜂巢状有源配电网构想、关键技术与展望

电力系统自动化 2019年第17期43卷 1-11页

作者：江道灼徐宁江崇熙胡列翔朱炳铨浙江大学电气工程学院浙江省杭州市310027 国网浙江省电力有限公司电力科学研究院浙江省杭州市310014 国网浙江省电力有限公司浙江省杭州市310007

传统拓扑结构的配电网难以适应大规模可再生能源的接入,而微网技术的规模化应用已成为未来分布式有源智能配电网的发展方向。文中提出了一种蜂巢状有源配电网拓扑,将“源网荷储”规范化配置的分布式微网群通过智能功率/信息交换基站以... 详细信息

传统拓扑结构的配电网难以适应大规模可再生能源的接入,而微网技术的规模化应用已成为未来分布式有源智能配电网的发展方向。文中提出了一种蜂巢状有源配电网拓扑,将“源网荷储”规范化配置的分布式微网群通过智能功率/信息交换基站以蜂巢状结构组合成网,实现微网的区域自治和相互间的广域互联;在对比其他变形拓扑的基础上,认为蜂巢状有源配电网是一种值得深入研究的未来智能配电网优选拓扑结构。对所提拓扑的实现方式和关键技术展开了探索性研究,初步建立了基于分层控制的通信机制、基站的经济运营模型和微网的经济调度模型。与现有配电网方案对比分析后,认为所提拓扑具有网络架构坚强、清洁能源消纳能力强、配电方式灵活和支持电力市场交易等优点。最后,分析了该新型配电网拓扑在经济性和技术性等方面面临的挑战并展望了下一步的研究方向。

关键词：蜂巢状有源配电网新型拓扑智能功率/信息交换基站微网(微电网) 关键技术

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胆汁酸诱导的肠道激素与肝脏糖代谢

中国糖尿病杂志 2015年第12期23卷 1134-1137页

作者：曹宏伟姜崴哈尔滨医科大学附属第一医院内分泌科 150001

胆汁酸是胆汁的主要成分,其主要作用是维持体内胆固醇代谢的平衡和促进肠道脂肪的消化。近年,胆汁酸作为一种信号分子,对葡萄糖、脂质及能量代谢的调节作用日趋受到重视。胆汁酸在肠道重吸收时分别通过核受体法呢醇X受体(FXR)、膜受体T... 详细信息

胆汁酸是胆汁的主要成分,其主要作用是维持体内胆固醇代谢的平衡和促进肠道脂肪的消化。近年,胆汁酸作为一种信号分子,对葡萄糖、脂质及能量代谢的调节作用日趋受到重视。胆汁酸在肠道重吸收时分别通过核受体法呢醇X受体(FXR)、膜受体TGR5促进肠道细胞分泌成纤维细胞生长因子19(FGF19)及胰升血糖素样肽-1(GLP-1)。FGF19和GLP-1通过不同的作用途径影响肝脏的糖代谢。本文就胆汁酸诱导的肠道激素与肝脏糖代谢的关系及研究进展进行综述。

关键词：胆汁酸成纤维细胞生长因子19 胰升血糖素样肽-1 肝脏葡萄糖代谢

Switching Frequency Improvement of a High Speed on/off Valve Based on Pre-excitation Control Algorithm

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Chinese Journal of Mechanical Engineering 2024年第4期37卷 94-106页

作者： Qi zh.ng Xiaotian Li Yongxin Mao Enguang Xu Tiwei jia Yanbiao Li Huayong Yang College of Mechanical Engineering Zhejiang University of TechnologyHangzhou 310023China State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou 310027China Key Laboratory of Special Purpose Equipment and Advanced Processing Technology Ministry of Education and Zhejiang ProvinceZhejiang University of TechnologyHangzhou 310023China

The high-speed on/off valve(HSV)serves as the fundamental component responsible for generating discrete fluids within digital hydraulic *** the switching frequency of the HSV increases,the properties of the generated discrete fluid approach those of continuous ***,a higher frequency response characteristic of HSV is the key to ensure the control accuracy of digital hydraulic ***,the current research mainly focuses on its dynamic performance,but neglect its *** paper presents a theoretical analysis demonstrating that the FRC of the HSV can be enhanced by minimizing its switching *** maximum switching frequency(MSF)is mainly determined by opening dynamic performance when HSV operates with low switching duty ratio(SDR),whereas the closing dynamic performance limits the MSF when HSV operates with high *** upon these findings,the pre-excitation control algorithm(PECA)is proposed to reduce the switching time of the HSV,and consequently enhance its *** results demonstrate that PECA shortens the opening delay time of HSV by 1.12 ms,the closing delay time by 2.54 ms,and the closing moving time by 0.47 ms in comparison to the existing advanced control *** a result,a larger MSF of 417 Hz and a wider controllable SDR range from 20%to 70%were achieved at a switching frequency of 250 ***,the proposed PFCA in this paper has been verified as an effective and promising approach for enhancing the control performance of digital hydraulic systems.

关键词： High speed on/off valve Switching frequency Pre-excitation Dynamic performance Switching duty ratio

侧卧位并仰卧位（后外侧并内侧）双切口治疗三踝骨折

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医学临床研究 2015年第6期32卷 1172-1174页

作者：张梅刃蒋际钊黄辉春广东省中医院珠海医院骨三科广东珠海519000

【目的】探讨侧卧下后外侧切口入路并仰卧位下内侧切口入路治疗三踝骨折的方法及疗效。【方法】应用侧卧下后内侧切口入路并仰卧位下内侧切口入路治疗三踝骨折21例，手术采用先侧卧位下后外侧切口入路，先复位固定外踝，在直视下来复位... 详细信息

【目的】探讨侧卧下后外侧切口入路并仰卧位下内侧切口入路治疗三踝骨折的方法及疗效。【方法】应用侧卧下后内侧切口入路并仰卧位下内侧切口入路治疗三踝骨折21例，手术采用先侧卧位下后外侧切口入路，先复位固定外踝，在直视下来复位固定后踝骨折块，然后改为仰卧位内侧切口固定内踝。根据Lange‐Hansen 分型：Ⅳ度旋后‐外旋型18例，Ⅳ度旋前‐外旋型3例。【结果】21例随访6-33个月，平均21个月。根据 Mazur 踝关节评分系统：优13例，良 3例，可5例。【结论】侧卧下后外侧切口入路＋仰卧位下内侧切口入路治疗治疗三踝骨折，直视下复位固定后踝骨折块，可以获得良好的复位和临床疗效。

关键词：体位仰卧位骨折/外科学踝损伤/外科学

Recognition of abnormal body surface characteristics of oplegnathus punctatus

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Information Processing in Agriculture 2022年第4期9卷 575-585页

作者： Beibei Li Jun Yue Shixiang jia Qing Wang zhenbo Li zhenzh.ng Li School of Information and Electrical Engineering Ludong UniversityYantai 264025China School of Civil Engineering Ludong UniversityYantai 264025China School of Information and Electrical Engineering China Agricultural UniversityBeijing 100083China Shandong Dongrun Instrument Technology Co. Ltd.264000China

To identify the abnormal characteristics of the oplegnathus punctatus is great importance to the detection of iridovirus disease in the breeding *** this paper,an advanced neural network model to identify the characteristics of the oplegnathus puncta-tus and predict its different periods of suffering from iridovirus disease is proposed based on the establishment of a data *** of all,a standard format data set of oplegnathus punctatus and an abnormal format date set are established in order to verify the effective-ness of the method in this *** then,the feature extraction fusion method is used for preprocessing in terms of the abnormal format data set,which combines the edge fea-tures extracted by the improved multi-template Sobel operator and the color features extracted by the HSV ***,an improved VGG-GoogleNet network recognition model comes into being through the fusion and improvement of the VGG and GoogleNet neural network *** experiments results show that the prediction accuracy rate for oplegnathus punctatus suffering from iridovirus disease in the the abnormal format data set and the standard format data set are improved,which reach 98.55%and 69.18%.

关键词： Oplegnathus punctatus Body surface characteristics Red seabream iridovirus Abnormal recognition Transfer learning