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Effects of cryptotanshinone on immune functions in rats with adjuvant arthritis

Chinese Medical Journal 2009年第24期122卷 3039-3042页

作者： ZHENG Fu-lin CHANG Yan jia Xiao-yi WEI Wei Department of Pharmacology Medical College Anhui University of Science and Technology Huainan Anhui 232001 China Institute of Clinical Pharmacology Anhui Medical University Hefei Anhui 230032 China

Background Cryptotanshinone （CT） was originally isolated from the dried roots of Salvia militorrhiza, an herb that is used extensively in Asian medicine and the extracts of this herb have been used in the treatment of several pathologies, including cardiovascular diseases, hematological abnormalities, hepatitis, and hyperlipidemia, but no studies had been carried on the treatment for rheumatic diseases with it. This study aimed to investigate the effects of cryptotanshinone on immune functions in rats with adjuvant arthritis （AA）. Methods Complete Freund＇s adjuvant was used to induce AA in rats. Thymus and spleen was aseptically taken from normal rats and the AA rats. Then a thymus lymphoid cell suspension, splenic lymphoid cell suspension and peritoneal macrophage cell suspension were prepared. After adding CT （0.1 ug/ml, 1.0 ug/ml, 10 ug/ml, 100 ug/ml, 1000 ug/ml） into the suspension, T and B lymphocytes proliferation was determined by 3-（4,5-2 dimethylthiazal-2yl）2,5- diphenyltetrazoliumbromide （MTT） assay. And the activities of interleukin-1 （IL-1） and IL-2 were measured by the mouse lymphocytes proliferation assay. Results Thymic T and splenic B lymphocyte proliferation of the AA rat was significantly lower, and could be stored through using CT in vitro. CT （100ug/ml and 1000ug/ml） increased T or B lymphocytes proliferation in vitro （P 〈0.01）. In AA rats, the levels of IL-1 released by abdominal PMφ significantly increased whereas the level of IL-2 released by T cells decreased in vitro. CT （1000 pg/ml） decreased the production of IL-1 and promoted production of IL-2 in vitro （P 〈0.05）. Conclusions CT can ameliorate the abnormal immunological functions in AA rats.

关键词： cryptotanshinone adjuvant arthritis immunopharmacological effect," rat

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Microstructural damage pattern of vascular cognitive impairment: a comparison between moyamoya disease and cerebrovascular atherosclerotic disease

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Neural Regeneration Research 2019年第5期14卷 858-867页

作者： jia-Bin Su Si-Da Xi Shu-Yi Zhou Xin Zhang Shen-Hong jiang Bin Xu Liang Chen Yu Lei Chao Gao Yu-Xiang Gu Department of Neurosurgery Huashan Hospital Fudan University Shanghai Medical College Fudan University Department of Radiology Huashan Hospital Fudan University

Moyamoya disease and cerebrovascular atherosclerotic disease are both chronic ischemic diseases with similar presentations of vascular cognitive impairment. The aim of the present study was to investigate the patterns of microstructural damage associated with vascular cognitive impairment in the two diseases. The study recruited 34 patients with moyamoya disease(age 43.9 ± 9.2 years; 20 men and 14 women, 27 patients with cerebrovascular atherosclerotic disease(age: 44.6 ± 7.6 years; 17 men and 10 women), and 31 normal controls(age 43.6 ± 7.3 years; 18 men and 13 women) from Huashan Hospital of Fudan University in China. Cognitive function was assessed using the Mini-Mental State Examination, long-term delayed recall of Auditory Verbal Learning Test, Trail Making Test Part B, and the Symbol Digit Modalities Test. Single-photon emission-computed tomography was used to examine cerebral perfusion. Voxel-based morphometry and tract-based spatial statistics were performed to identify regions of gray matter atrophy and white matter deterioration in patients and normal controls. The results demonstrated that the severity of cognitive impairment was similar between the two diseases in all tested domains. Patients with moyamoya disease and those with cerebrovascular atherosclerotic disease suffered from disturbed supratentorial hemodynamics. Gray matter atrophy in bilateral middle cingulate cortex and parts of the frontal gyrus was prominent in both diseases, but in general, was more severe and more diffuse in those with moyamoya disease. White matter deterioration was significant for both diseases in the genu and body of corpus callosum, in the anterior and superior corona radiation, and in the posterior thalamic radiation, but in moyamoya disease, it was more diffuse and more severe. Vascular cognitive impairment was associated with regional microstructural damage, with a potential link between, gray and white matter damage. Overall, these results provide insight into the p

关键词： nerve regeneration vascular cognitive impairment moyamoya disease cerebrovascular atherosclerotic disease magnetic resonance imaging diffusion tensor imaging gray matter volume tract-based spatial statistics single-photon emission computed tomography neural regeneration

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Tetrandrine suppresses proliferation, induces apoptosis, and inhibits migration and invasion in human prostate cancer cells

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Asian Journal of Andrology 2015年第5期17卷 850-853,I0011页

作者： Wei Liu Bo KOU Zhen-Kun Ma Xiao-Shuang Tang Chuan Lv Min Ye jia-Qi Chen Lei Li Xin-Yang Wang Da-Lin He Department of Urology First Affiliated Hospital of Medical School Xi'an Jiaotong University Xi'an 710061 China Oncology Research Lab Key Laboratory of Environment and Genes Related to Diseases Ministry of Education of the People's Republic of China Xi'an 710061 China Department of Urology First People's Hospital of Jining Jining 272100 China Ophthalmology department The First Affiliated Hospital of Medical School Xi'an Jiaotong University Xi'an 710061 China

Tetrandrine （TET）, a traditional Chinese medicine, exerts remarkable anticancer activity on various cancer cells. However, little is known about the effect of TET on human prostate cancer cells, and the mechanism of function of TET on prostate cancer has not yet been elucidated. To investigate the effects of TET on the suppression of proliferation, induction of apoptosis, and inhibition of migration and invasion in human prostate cancer cell lines, DU145 and PC-3. Inhibition of growth was determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay and clone formation assay, and flow cytometry analysis was performed to detect the induction of apoptosis. Activation of poly （ADP-ribose） polymerase, caspase-3, Akt, phospho-Akt, Bcl-2, and Bax was analyzed by Western blotting. Wound healing assay and transwell migration assay were used to evaluate the effect of TET on migration and invasion of cancer cells. TET inhibited the growth of DU145 and PC-3 cells in a dose- and time-dependent manner. Cell cloning was inhibited in the presence of TET in DU145 and PC-3 cells. TET suppressed the migration of DU145 and PC-3 cells. Transwell invasion assay showed that TET significantly weakened invasion capacity of DU 145 and PC-3 cells. TET exhibited strong inhibitory effect on proliferation, migration, and invasion of prostate cancer cells. In addition, TET induced apoptosis in a dose-dependent manner by activating the caspase cascade and inhibiting phosphoinositide 3-kinase-Akt signal pathway. The accumulating evidence suggests that TET could be a potential therapeutic candidate against prostate cancer in a clinical setting.

关键词： apoptosis invasion migration proliferation prostate cancer tetrandrine

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Effects and mechanisms of Cryptotanshinone on rats with adjuvant arthritis

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Chinese Medical Journal 2011年第24期124卷 4293-4298页

作者： ZHENG Fu-lin CHANG Yan jia Xiao-yi HUANG Min WEI Wei Department of Phar-macology Medical College Anhui Universityof Science and Technology Huainan Anhui 232001 China Institute of Clinical Pharmacology Anhui Medical UniversityHefei Anhui 230032 China School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou Guangdong 510006 China

Background Cryptotanshinone （CT） is the major active constituent of Salvia miltiorrhiza Bunge. The present study was carried out to investigate the effects of CT on rats with adjuvant arthritis （AA）. Methods AA was induced by the metatarsal footpad injection with complete Freund＇s adjuvant in male Sprague-Dawley rats. The secondary inflammatory reaction was evaluated by hind paw swelling and the polyarthritis index. Activity of interleukin-1 （IL-1） was detected by the concanavalin A-induced thymocytes proliferation assay. The lymphocytes proliferation and IL-2 production were assayed by 3-（4,5-2dimethylthiazal-2yl）2,5-diphenyltetrazoliumbromide （MTT） and activated mouse splenocytes proliferation, respectively. Results Intragastric administration of CT （50 and 100 mg/kg） significantly decreased secondary inflammatory reactions and increased the spleen and thymus index. There was a marked immunologic and inflammatory response in the AA model, which was accompanied by the decrease of thymocyte proliferation and IL-2 production as well as the increase of IL-1 production. CT apparently enhanced thymocyte proliferation and decreased IL-1 production in AA rats. Conclusion These results indicate that CT may exert its anti-inflammatory and immunoregulatory effects through inhibiting lymphocyte proliferation and production of pro-inflammatory mediators.

关键词： cryptotanshinone adjuvant arthritis lymphocyte proliferation cytokine

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Contragestazol (DL111-IT) inhibits proliferation of human androgen-independent prostate cancer cell line PC3 in vitro and in vivo

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Asian Journal of Andrology 2005年第4期7卷 389-393,页

作者： Qiao-Jun He Bo Yang Yi-jia Lou Rui-Ying Fang Department of Pharmacology College of Pharmaceutical Science Zhejiang University Hangzhou 310031 China

Aim： To evaluate the antiproliferative activity of contragestazol （DL111-IT） on the human prostate cancer cell line PC3 in vitro and in vivo and to elucidate its potential molecular mechanisms. Methods： The cell killing ability of DL111-IT was measured by the 3-（4,5-dimethylthia-zol,2-yl）-2,5-diphenyltetrazolium bromide （MTT） reagent assay method and the tumor xenograft model. The cell cycle was analyzed by flow cytometry and protein expression, including retinoblastoma （pRb）, cyclin-dependent kinase 4 （CDK4） and cyclin D 1, was detected by Western blotting. Results： DL111-IT exhibited high efficiency on cell growth inhibition of the human androgen-independent prostate cancer cell line PC3. The drug concentration that yielded 50 % cell inhibition （IC50 value） was 9.9 mg/mL. In the PC3 tumor xenograft study, DL111-IT （1.25 mg/kg-20.0 mg/kg） given once a day for 10 days significantly inhibited tumor growth, with the inhibition rate ranging from 21% to 50 %. Flow cytometric analysis indicated that DL111-IT could cause GI arrest in the PC3 cell line, but not apoptosis. DL111-IT enhanced pRb expression and down-regulated CDK4 and cyclin D 1 expression, suggesting that cell cycle regulation might contribute to the anticancer property of DL 111- IT. Conclusion： DL111-1T inhibits the proliferation of human androgen-independent prostate cancer cell line PC3 in vitro and in vivo by a cell cycle regulation pathway.

关键词： DL111-IT prostate cancer pRb cyclin-dependent kinase 4 cyclin D 1 PC3 cell line

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Robot-assisted Living-donor Left Lateral Sectionectomy

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Chinese Medical Journal 2017年第7期130卷 874-876页

作者： Ming-Heng Liao jia-Yin Yang Hong Wu Yong Zeng Department of Liver Surgery Liver Transplantation Division West China Hospital Sichuan University Chengdu Sichuan 610041 China

INTRODUCTION In 2002, Cherqui et al. reported the first purely laparoscopic living-donor left lateral sectionectomy （LDLLS）yl This technique has now become a standardized procedure and has significantly shortened donor *** The da Vinci robotic surgical system was introduced into the field of liver surgery more than 10 years ago. Its flexible mechanical ＂wrist＂ and stable three-dimensional （3D） visual field help minimize risks from complicated procedures.

关键词： Laparoscopic Surgery Liver Transplantation Living Donor Robotic Surgery Sectionectomy

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含肟基的Schiff碱Cu(Ⅱ)和Ni(Ⅱ)配合物的合成、超分子结构和光谱性质（英文）

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无机化学学报 2018年第12期34卷 2261-2270页

作者：张宏佳常健贾浩然孙银霞兰州交通大学化学与生物工程学院兰州730070

合成了2个含肟基Schiff碱的Cu(Ⅱ)和Ni(Ⅱ)配合物[Cu(L1)2]·(1,4-dioxane)(1)和[Ni(L2)2](2),并通过元素分析、红外光谱、紫外可见光谱,荧光光谱及X射线单晶衍射分析进行了表征和分析。结果表明,配合物1和2均包含1个中心金属离子和2个... 详细信息

合成了2个含肟基Schiff碱的Cu(Ⅱ)和Ni(Ⅱ)配合物[Cu(L1)2]·(1,4-dioxane)(1)和[Ni(L2)2](2),并通过元素分析、红外光谱、紫外可见光谱,荧光光谱及X射线单晶衍射分析进行了表征和分析。结果表明,配合物1和2均包含1个中心金属离子和2个双齿配体单元,且金属离子的配位数均为4,均具有轻微扭曲的平面四边形几何构型。不同的是配合物1通过分子间氢键以及π…π堆积作用相连接形成了三维超分子结构,而配合物2仅通过分子间的π…π堆积作用形成了一条一维超分子链。

关键词： Schiff碱配体 Cu(Ⅱ)和Ni(Ⅱ)配合物超分子结构荧光性质

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Single-cell Transcriptomic Analysis Reveals the Cellular Heterogeneity of Mesenchymal Stem Cells

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 70-86页

作者： Chen Zhang Xueshuai Han Jingkun Liu Lei Chen Ying Lei Kunying Chen jia Si Tian-yi Wang Hui Zhou Xiaoyun Zhao Xiaohui Zhang Yihua An Yueying Li Qian-Fei Wang CAS Key Laboratory of Genomic and Precision Medicine Collaborative Innovation Center of Genetics and DevelopmentBeijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China China National Center for Bioinformation Beijing 100101China University of Chinese Academy of Sciences Beijing 100049China Department of Medical Experimental Center Qilu Hospital(Qingdao)Cheeloo College of MedicineShandong UniversityQingdao 266035China Qingdao Key Lab of Mitochondrial Medicine Qingdao 266035China International Department Liangxiang CampusBeijing University of Chinese MedicineBeijing 102401China Yihua Biotechnology Co. Ltd.Beijing 100041China Peking University People’s Hospital Peking University Institute of HematologyBeijing 100044China Department of Functional Neurosurgery Third Medical CenterGeneral Hospital of Chinese PLABeijing 100039China

Ex vivo-expanded mesenchymal stem cells(MSCs)have been demonstrated to be a heterogeneous mixture of cells exhibiting varying proliferative,multipotential,and immunomodulatory ***,the exact characteristics of MSCs remain largely *** singlecell RNA sequencing of 61,296 MSCs derived from bone marrow and Wharton’s jelly,we revealed five distinct *** developmental trajectory of these five MSC subpopulations was mapped,revealing a differentiation path from stem-like active proliferative cells(APCs)to multipotent progenitor cells,followed by branching into two paths:1)unipotent preadipocytes or 2)bipotent prechondro-osteoblasts that were subsequently differentiated into unipotent *** stem-like APCs,expressing the perivascular mesodermal progenitor markers CSPG4/MCAM/NES,uniquely exhibited strong proliferation and stemness ***,the prechondrocyte subpopulation specifically expressed immunomodulatory genes and was able to suppress activated CD3^(+T) cell proliferation in vitro,supporting the role of this population in *** summary,our analysis mapped the heterogeneous subpopulations of MSCs and identified two subpopulations with potential functions in self-renewal and *** findings advance the definition of MSCs by identifying the specific functions of their heterogeneous cellular composition,allowing for more specific and effective MSC application through the purification of their functional subpopulations.

关键词： Mesenchymal stem cell Single-cell RNA sequencing Heterogeneity Lineage trajectory Immune regulation

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Synthesis and the Interaction of 2-(1H-pyrazol-4-yl)-1H-imidazo[4,5-f][1,10]phenanthrolines with Telomere DNA as antitumor agents

Synthesis and the Interaction of 2-(1H-pyrazol-4-yl)-1H-imid...

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中国化学会第30届学术年会-第二十八分会：化学生物学

作者： jia-Chun Liu Mei Cheng Wen Zhang Lab of Chemical Biology and Molecular Drug Design College of Pharmaceutical ScienceZhejiang University of Technology

A novel series of 2-(1H-pyrazol-4-yl)-lH-imidazo[4,5-f][1,10]phenanthrolines were designed,synthesized and evaluated for their antitumor activity against lung *** compounds showed potent antitumor activity against lung adenocarcinoma(A549/DDP),especially compound 12 c showed the most potential antitumor activity against A549/DDP cell with the half maximal inhibitory concentration(IC) value at 1.65 ± 0.13 μmol/***,compounds 11 c,12c and 13 c exhibited significant telomere DNA interactions to stablize telomeric DNA G-quadruplexs with ΔT values increased from 12.4 to 16.3 ℃ at 10 μM concentration level,suggesting that these phenanthroline derivatives could stabilize telomere *** work may make a contribution to the development of drug targeting DNA for lung cancer.

关键词： Phenanthroline Pyrazole Telomere DNA interaction Antitumor

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“有本字假借”产生原因述评

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黔东南民族师范高等专科学校学报 2005年第5期23卷 97-98,100页

作者：吉益民宿迁学院教育系江苏宿迁223800

尝试对语言学界争议颇多的“有本字假借”问题进行了探讨,从中发现“有本字假借”可以分为两种类型:一种是由于古书传抄中的讹误等原因而形成的真通假;另一种是由于淆乱了假借字产生的历时和共时关系而形成的伪通假,进而强调在研究假借... 详细信息

尝试对语言学界争议颇多的“有本字假借”问题进行了探讨,从中发现“有本字假借”可以分为两种类型:一种是由于古书传抄中的讹误等原因而形成的真通假;另一种是由于淆乱了假借字产生的历时和共时关系而形成的伪通假,进而强调在研究假借的生成缘由及其性质时应该严格区分这一点。

关键词：有本字假借真通假伪通假

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