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Recombinant Mouse Canstatin Inhibits Chicken Embryo Chorioallantoic Membrane Angiogenesis and Endothelial Cell Proliferation

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 845-850页

作者： Hou WH Wang TY Yuan BM Chai YR jia yl Tian F Wang JM Xue LX Laboratory for Cell Biology Zhengzhou University Zhengzhou 450052 China

Human canstatin, a 24 kD fragment of the alpha2 chain of type IV collagen, has been proved to be one of the most effective inhibitors of angiogenesis and tumor growth. To investigate in vivo antiangiogenesis activity and in vitro effects on endothelial cell proliferation of recombinant mouse canstatin, the cDNA of mouse canstatin was introduced into an expression vector pQE40 to construct a prokaryotic expression vector pQE-mCan. The recombinant mouse canstatin efficiently expressed in E. coli M15 after IPTG induction was monitored by SDS-PAGE and by Western blotting with an anti-hexahistidine tag antibody. The expressed mouse canstatin, mainly as inclusion bodies, accounted for approximately 35% of the total bacterial proteins. The inclusion bodies were washed, lysed and purified by the nickel affinity chromatography to a purity of approximately 93%. The refolded mouse canstatin was tested on the chicken embryo chorioallantoic membranes (CAM), and a large number of newly formed blood vessels were significantly regressed. In addition, recombinant mouse canstatin potently inhibited endothelial cell proliferation with no inhibition on non-endothelial cells. Taken together, these findings demonstrate that the recombinant mouse canstatin effectively inhibited angiogenesis of the chicken embryo in a dose-dependent manner and specially suppressed in vitro the proliferation of human umbilical vein endothelial cells.

关键词： Angiogenesis Inhibitors Animals Blotting Western Cell Proliferation Chick Embryo Chorioallantoic Membrane Collagen Type IV Endothelial Cells Endothelium Vascular Humans Mice Molecular Sequence Data Neovascularization Physiologic Peptide Fragments Recombinant Proteins Research Support Non-U.S. Gov't

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转变兽药行政审批理念提高兽药行政审批效能

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中国兽药杂志 2007年第5期41卷 1-4页

作者：贾幼陵农业部兽医局北京100026

兽药行政审批工作,是法律设定的具有强制力的政府行为,对管理相对人和生产者、畜产品消费者的利益影响重大,处理不好,很容易引发社会矛盾。兽药管理在管理制度、管理内容和管理形式上,与人用药品管理都有很多相似之处,国家食品药品监督... 详细信息

兽药行政审批工作,是法律设定的具有强制力的政府行为,对管理相对人和生产者、畜产品消费者的利益影响重大,处理不好,很容易引发社会矛盾。兽药管理在管理制度、管理内容和管理形式上,与人用药品管理都有很多相似之处,国家食品药品监督管理局腐败案件的发生,给我们敲响了警钟。本文结合近年来兽药管理工作实践,借鉴发达国家的先进管理理念,从树立先进审批理念、严格审批程序和建立完善权力运行机制等3个方面着重阐述了兽药行政审批工作应坚持以科学发展观为指导,转变审批理念,完善审批程序,提高审批效能。

关键词：行政审批兽药管理国家食品药品监督管理局管理制度审批工作审批程序科学发展观政府行为

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国家首席兽医官贾幼陵在国务院新闻办新闻发布会上的讲话

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中国兽药杂志 2005年第11期39卷 1-2页

作者：贾幼陵国家首席兽医官

女士们、先生们:今秋以来,经国家禽流感参考实验室确诊,我国内蒙古自治区、安徽省、湖南省先后各发生一起H5N1亚型高致病性禽流感疫情.

关键词：国务院新闻办新闻发布会高致病性禽流感疫情贾幼陵兽医内蒙古自治区 H5N1亚型实验室安徽省

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Synthesis of proline fullerene derivative

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Chinese Science Bulletin 1997年第16期42卷 1360-1363页

作者： Zhao, yl Gan, LB Zhou, DJ Luo, CP Huang, CH jia, GC Wu, T Peking Univ State Key Lab Rare Earth Mat Chem & Applicat Beijing 100871 Peoples R China Hong Kong Univ Sci & Technol Hong Kong Hong Kong Peoples R China Inst Beijing Microchem Beijing 100091 Peoples R China

THE unique spherical nature and potential application of fullerene have attracted great attention. Their functionalization is in particular an active research field. Many methods are now available for the preparation of fullerene derivatives. The 1, 3-dipole cycloaddition of ylide to fullerene is one of the best known methods. We have recently reported that aminoacid esters can be added to C60 to form fulleropyrrolidine derivatives under photolysis. Our in-

关键词： fullerene pyrrolidine stereoisomer.

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Expression of co-stimulator 4-1BB molecule in hepatocellular carcinoma and adjacent non-tumor liver tissue,and its possible role in tumor immunity

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World Journal of Gastroenterology 2004年第2期10卷 195-199页

作者： Wan, yl Zheng, SS Zhao, ZC Li, MW jia, CK Zhang, H Zhejiang Univ Sch MedKey Lab Combined Multiorgan Transplantat Affiliated Hosp 1Minist Publ Hlth Dept Hepatobiliary & Pancreat Surg Hangzhou 310000 Zhejiang Prov Peoples R China

AIM:To investigate the expression of 4-1BB molecule in hepatocellular carcinoma (HCC) and its adjacent ***:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in hepatocarcinoma and its adjacent tissues,and peripheral blood mononuclear cells (PBMCs) from both HCC and health control *** cytometry was used to analyse the phenotypes of T cell subsets from the blood of HCC patients and healthy volunteers,and further to determine whether 4-1BB molecules were also expressed on the surface of CD4+ and CD8+ T cells. The localization of 4-1BB proteins on tumor infiltrating T cells was determined by direct immunofluorescence cytochemical staining and detected by confocal ***:4-1BB mRNA, which was not detectable in normal liver,was found in 19 liver tissues adjacent to tumor edge(0.05,respectively),while a significant lower percentage of CD3^+ T cell was found in HCC group as compared to healthy control group (P<0.05).However, 4-1BB molecules were almost not found on the surface of CD4+ and CD8+ T cells in HCC and healthy control ***-staining of 4-1BB^+/CD4^+ and 4-1BB^+/CD8^+ immunofluorescence on tumor infiltrating T cells was detected in 13 liver tissues adjacent to tumor edge (<1.0cm) by confocal ***: Although HCC may escape from immune attack by weak immunogenicity or downregulated expression of MHC-1 molecules on the tumor cell surface,tumor infiltrating T cells can be activated via other costimulatory signal pathways to exert a limited antitumor effect on local microenvironment. The present study also implicates that modulating 4-1BB/4-1BBL costimulatory pa

关键词：肝细胞癌肿瘤免疫学 4-1BB基因分子机制逆转录聚合酶链反应 T淋巴细胞

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Hybrid Control Strategy for Air-conditioning Loads Participating in Peak Load Reduction Through Wide-range Transport Model

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Journal of Modern Power Systems and Clean Energy 2022年第6期10卷 1542-1551页

作者： Yunfeng Ma Zengqiang Mi Ruifeng Zhang Huowen Peng Yulong jia School of Electrical&Electronic Engineering North China Electric Power UniversityBeijingChina Department of Electrical Engineering North China Electric Power UniversityBaodingChina University of Edinburgh Old CollegeSouth BridgeEdinburghEH89YLUK Planning&Design Institute at State Grid Integrated Energy Services Group Co. Ltd.Beijing 100032China

This paper proposes a hybrid control strategy of air-conditioning loads(ACLs)for participating in peak load *** hybrid control strategy combines the temperature setpoint adjustment(TSA)control and on/off control together to make full use of response potentials of *** primary free transport model of ACLs has been established in literature at or near a fixed temperature *** this paper,a wide-range transport(WRT)model suitable for larger value of TSA is *** WRT model can be constructed easily through the parameter of devices and indoor and outdoor *** modulate the aggregate response characteristics of ACLs more friendly to the power grid,the safe protocol(SP)is adopted and integrated into the WRT model,which achieves a good unification of oscillation suppression and efficient ***,the hybrid control strategy is implemented based on the WRT model,and the model predictive control(MPC)controller is designed considering the tracking error and control switch *** last,the superiority of the hybrid control strategy is verified and the performance of ACLs for peak load reduction under this controller is *** simulation results show that the hybrid control strategy could exploit the load reduction potential of ACLs fully than the TSA mode and track the reference signal more accurately.

关键词： Peak load reduction air conditioning load aggregate modeling response characteristics state space model model predictive control

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Crystalline-State Solvent:Metal-Organic Frameworks as a Platform for Intercepting Aggregation-Caused Quenching

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Chinese Journal of Chemistry 2022年第5期40卷 589-596页

作者： Yan-Yuan jia jia-Cheng Yin Na Li Ying-Hui Zhang Rui Feng Zhao-Quan Yao Xian-He Bu School of Materials Science and Engineering Tianjin Key Laboratory of Metal and Molecule-Based Material ChemistryNankai UniversityTianjin300350 China State Key Laboratory of Elemento-Organic Chemistry College of ChemistryNankai UniversityTianjin300071 China

The sequestration of organic luminescent molecules(OLMs)within cage-based metal-organic frameworks(MOFs)as a dispersion platform has been developed to impede aggregation-caused quenching(ACQ).The homogenous encapsulation of distinct luminescent guests of different sizes and emissive behaviors in the cage structure of a MOF resulted in high fluorescent quantum yields of 44.8%for DAPI@NKU-110(DAPI=4',6-diamidino-2-phenylindole),65.4%for TPPA@NKU-110(TPPA=tris(4-(pyridin-4-yl)phenyl)amine),31.3%for R6G@NKU-110(R6G=Rhodamine 6G),and 58.3%for PY@NKU-110(PY=Pyronin Y),attributable to the confinement effect caused by the rigid cages of *** significantly,a positive correlation of the encapsulated quantity of OLMs with their concentration in the in-situ solvothermal reaction was unveiled by spectral analysis and utilized to facilely fabricate a white-light-emitting crystal material TPPA+R6G@*** material features a large crystal size on the millimeter-scale,broadband white emission,ideal CIE coordinates(0.33,0.34),and a high quantum yield(49.1%)when excited at 365 ***,such a strategy can be easily generalized to an abundance of other cage-based MOFs and a plentiful volume of OLMs for the future development of colorful,high performance luminescent materials.

关键词： Metal-organic frameworks Cage compounds Fluorescence Color tunability In-situ encapsulation

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Synthesis and biological evaluation of 3-(piperidin-4-yl)isoxazolo[4,5-d]pyrimidine derivatives as novel PI3Kδ inhibitors

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Chinese Chemical Letters 2015年第10期26卷 1283-1288页

作者： jia-Lin Guo Yun-Yong Liu Ya-Zhong Pei The Center for Combinatorial Chemistry and Drug Discovery of Jilin University School of Pharmaceutical Sciences Jilin University Nanjing Gator Medi Tech Company Ltd.

An efficient synthesis of novel 3-（piperidin-4-yl）isoxazolo[4,5-d]pyrimidine scaffold has been designed and deveopled. A series of 5-phenylurea derivatives was synthesized using this method. Their cytotoxic activities against breast cancer cell line BT-474 were evaluated by CCK-8 assay. Most of them showed potent anti-proliferative activities, of which compound 20 and 21 exhibited IC50 s of 1.565 mmol/L and1.311 mmol/L, respectively. Furthermore, compound 20 and 21 also showed potent inhibitory activities against PI3 Kd with IC50 s of 0.286 mmol/L and 0.452 mmol/L, respectively. These results indicate that these 3-（piperidin-4-yl）isoxazolo[4,5-d]pyrimidine derivatives are novel antitumor agents through the inhibition of PI3 Kd.

关键词： Synthesis Isoxazolopyrimidine PI3Kδ inhibitors Cytotoxicity

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Methodology of network pharmacology for research on Chinese herbal medicine against COVID-19:A review

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Journal of Integrative Medicine 2022年第6期20卷 477-487页

作者： Yi-xuan Wang Zhen Yang Wen-xiao Wang Yu-xi Huang Qiao Zhang jia-jia Li Yu-ping Tang Shi-jun Yue Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation)and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources IndustrializationShaanxi University of Chinese MedicineXi’an 712046Shaanxi ProvinceChina Department of Scientific Research Shaanxi Provincial People’s HospitalXi’an 710068Shaanxi ProvinceChina

Traditional Chinese medicine,as a complementary and alternative medicine,has been practiced for thousands of years in China and possesses remarkable clinical ***,systematic analysis and examination of the mechanistic links between Chinese herbal medicine(CHM)and the complex human body can benefit contemporary understandings by carrying out qualitative and quantitative *** increasing attention,the approach of network pharmacology has begun to unveil the mystery of CHM by constructing the heterogeneous network relationship of“herb-compound-target-pathway,”which corresponds to the holistic mechanisms of *** integrating computational techniques into network pharmacology,the efficiency and accuracy of active compound screening and target fishing have been improved at an unprecedented *** review dissects the core innovations to the network pharmacology approach that were developed in the years since 2015 and highlights how this tool has been applied to understanding the coronavirus disease 2019 and refining the clinical use of CHM to combat it.

关键词： Chinese traditional medicine Herbal medicine Network pharmacology Compound identification COVID-19

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Synthesis and biological evaluation of novel triazole derivatives as antifungal agents

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Chinese Chemical Letters 2013年第3期24卷 219-222页

作者： Hui Tang Can-Hui Zheng Xiao-Hui Ren jia Liu Na Liu jia-Guo Lv Ju Zhu You-Jun Zhou Pharmacy Provincial Hospital Affiliated to Shandong University School of Pharmacy Second Military Medical University

A series of 1-（benzylamino）-2-（2,4-difiuorophenyl）-3-（lH-1,2,4-triazol-l-yl）propan-2-ols compounds were synthesized and evaluated for their antifungal activities in vitro. The results showed that compounds 6A and 6B exhibited good antifungal activity. Compound 6tt8 showed the strongest antifungal activity, which was significantly higher than that of the lead compounds and positive-control drugs Fluconazole and Itraconazole. In particular, the antifungal activity of compound 6/18 against Candida albicans and Candida krusei （MIC80 both at 0.00097 p.g/mL） was 515 and 64 times that of Fluconazole, respectively. The structure-activity relationships of the synthesized compounds were discussed, and the docking model of the target compounds with fungal lanosterol 14a-demethylase （CYP51） was analyzed.

关键词： TriazoleAntifungalStructure-activity relationshipCYP51

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