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iTRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation

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Journal of Integrative Agriculture 2015年第7期14卷 1407-1413页

作者： MA lu BU Deng-pan YANG Yong-xing YAN Su-mei WANG jia-qi Inner Mongolia Agricultural University State Key Laboratory of Animal Nutrition/CAAS-ICRAF Joint Laboratory on Agroforestry and Sustainable Animal Husbandry/Institute of Animal Sciences Chinese Academy of Agricultural Sciences Synergetic Innovation Center of Food Safety and Nutrition Northeast Agricultural University

Dairy cows undergo tremendous changes in physiological, metabolism and the immune function from pregnancy to lac- tation that are associated with cows being susceptible to metabolic and infectious diseases. The objective of this study is to investigate the changes of plasma proteome on 21 d before expected calving and 1 d after calving from dairy cows using an integrated proteomic approach consisting of minor abundance protein enrichment by ProteoMiner beads, protein labeling by isobaric tags for relative and absolute quantification, and protein identification by liquid chromatography coupled with tandem mass spectrometry. Nineteen proteins were changed around the time of calving. These proteins were asso- ciated with response to stress, including acute-phase response and defense response, based on the proteins annotation. In particular, three up-regulated proteins after calving including factor V, a2-antiplasmin and prothrombin were assigned into the complement and coagulation pathway. These results may provide new information in elucidating host response to lactation and parturition stress, and inflammatory-like conditions at the protein level. Differential proteins may serve as potential markers to regulate the lactation and parturition stress in periparturient dairy cows.

关键词： dairy cow periparturient plasma proteomic quantitative analysis

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高压拉曼光谱研究均苯四甲酸二酐

光谱学与光谱分析 2024年第9期44卷 2434-2438页

作者：丛广宇李东飞刘嘉睿长春光华学院基础教研部吉林长春130033 长春师范大学物理学院吉林长春130032 吉林师范大学物理学院吉林四平136000

测量了常温常压下的均苯四甲酸二酐的拉曼光谱,并对所测量的各个拉曼谱线进行归属认证。获得了从常压到24GPa压强条件下的原位拉曼光谱,通过频率-压强关系曲线可以得出该材料在2Gpa存在晶体结构相变,定义了均苯四甲酸二酐在低于2GPa压... 详细信息

测量了常温常压下的均苯四甲酸二酐的拉曼光谱,并对所测量的各个拉曼谱线进行归属认证。获得了从常压到24GPa压强条件下的原位拉曼光谱,通过频率-压强关系曲线可以得出该材料在2Gpa存在晶体结构相变,定义了均苯四甲酸二酐在低于2GPa压强条件的晶体结构为相Ⅰ,高于2GPa压强条件的晶体结构相为相Ⅱ。在相变前后,绝大多数的拉曼谱线频率-压强曲线斜率变小,说明与相Ⅰ相比,各官能团对压强的敏感程度下降,相Ⅱ的分子晶体具有更紧密的堆积结构。拉曼光谱强度能够给出丰富的材料结构信息,通过对C—O伸缩振动的倍频(常压位于1837cm^(-1))和C=O伸缩振动基频(常压位于1865cm^(-1))的相对拉曼光谱强度随压强的变化规律进行分析,发现两拉曼谱线强度比在2GPa附近存在突变,为均苯四甲酸二酐在2GPa附近发生结构相变提供了实验依据。

关键词：拉曼光谱高压相变均苯四甲酸二酐

Strigolactone-Regulated Hypocotyl Elongation Is Dependent on Cryptochrome and Phytochrome Signaling Pathways in Arabidopsis

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Molecular Plant 2014年第3期7卷 528-540页

作者： Kun-Peng jia Qian luo Sheng-Bo He Xue-Dan lu Hong-Quan Yang Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of Agricultural and Biological Sciences Shanghai Jiaotong University800 Dongchuan Road Shanghai 200240 China Schoolof Life Sciences and Biotechnology Shanghai Jiaotong University. 800 Dongchuan Road Shanghai 200240 China

Seedling development including hypocotyl elongation is a critical phase in the plant life cycle. Light regula- tion of hypocotyl elongation is primarily mediated through the blue light photoreceptor cryptochrome and red/far-red light photoreceptor phytochrome signaling pathways, comprising regulators including COP1, HY5, and phytochrome- interacting factors （PIFs）. The novel phytohormones, strigolactones, also participate in regulating hypocotyl growth. However, how strigolactone coordinates with light and photoreceptors in the regulation of hypocotyl elongation is largely unclear. Here, we demonstrate that strigolactone inhibition of hypocotyl elongation is dependent on cryp- tochrome and phytochrome signaling pathways. The photoreceptor mutants cry1 cry2, phyA, and phyB are hyposensi- tive to strigolactone analog GR24 under the respective monochromatic light conditions, while cop1 and pifl pif3 pif4 pif5 （pifq） quadruple mutants are hypersensitive to GR24 in darkness. Genetic studies indicate that the enhanced respon- siveness of cop1 to GR24 is dependent on HY5 and MAX2, while that of pifq is independent of HY5. Further studies demonstrate that GR24 constitutively up-regulates HY5 expression in the dark and light, whereas GR24-promoted HY5 protein accumulation is light- and cryptochrome and phytochrome photoreceptor-dependent. These results suggest that the light dependency of strigolactone regulation of hypocotyl elongation is likely mediated through MAX2-dependent promotion of HY5 expression, light-dependent accumulation of HY5, and PIF-regulated components.

关键词： hypocotyl elongation cryptochrome phytochrome strigolactones COP1 HY5 PIF MAX2.

工程水泥基复合材料配合比对其拉伸性能及耗能特性的影响

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西南科技大学学报 2024年第2期39卷 23-31页

作者：黄辉张强吴芝忠贾彬陆冰西南科技大学土木与建筑工程学院四川绵阳621010 中国五冶集团市政工程分公司成都610056

工程水泥基复合材料(Engineered cementitious composites,ECC)在工程结构修复加固等领域应用广泛。制备了12组不同配比且不同养护龄期的ECC试件进行单轴拉伸试验,研究了不同砂胶比、水胶比、硅灰掺量、纤维掺量及养护龄期对ECC拉伸性... 详细信息

工程水泥基复合材料(Engineered cementitious composites,ECC)在工程结构修复加固等领域应用广泛。制备了12组不同配比且不同养护龄期的ECC试件进行单轴拉伸试验,研究了不同砂胶比、水胶比、硅灰掺量、纤维掺量及养护龄期对ECC拉伸性能及耗能特性的影响,优化了ECC的配合比范围。结果表明:ECC的初裂强度和峰值强度在水胶比的一定范围内随水胶比的降低而增加,水胶比的建议区间为0.24~0.30;砂胶比在一定范围内的增加会导致ECC的初裂强度和峰值强度显著提高,砂胶比增加且不超过0.66时断裂能、延性指数(耗能能力)均呈现线性增加,砂胶比的建议区间为0.60~0.66;纤维掺量增加对ECC的断裂能和裂纹扩展有一定提高,但会使强度降低,纤维掺量的建议值为1.6%;适当的硅灰掺量能有效增加抗拉应变能力。

关键词：工程水泥基复合材料配合比单轴拉伸试验断裂能延性指数

Estimation of soil erosion in the Xihanshui River Basin by using 137Cs technique

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International Journal of Sediment Research 2012年第4期27卷 486-497页

作者： Yan-hong jia Zhao-yin WANG Xiang-min ZHENG lu-jie HAN East China Normal University State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University State Key Laboratory of Hydroscience and Engineering of Tsinghua University

The Cesium-137 technique was used to estimate soil erosion in the Xihanshui River *** than 100 samples were taken from 10 sites and 20 hillslopes with a 10cm diameter hand-operated core *** sample was 60 cm ***137Cs activity was analyzed by gamma *** simplified mass balance model and the profile distribution model were used to calculate soil erosion and deposition *** local137Cs reference ranged from 1,600 to 2,402 Bq *** data shows an exponential decrease of mass concentration and amount with depth in an undisturbed soil *** erosion in the river basin is moderate or severe on cultivated land with annual erosion rates of 2,000-6,000 t *** general,very severe or severe soil erosion occurred at the upper slope sections,moderate or severe soil erosion at the middle section,and moderate or slight soil erosion at the lower slope *** the slopes with natural vegetation,consisting of herbaceous and wood species,the erosion rate is much lower or not *** the lower section of slopes with well-developed vegetation however,there was no soil loss,instead deposition occurred at a rate of more than 300 t *** slope gradient and vegetation cover affected soil erosion and deposition *** general,the rate of soil erosion was proportional to the slope gradient and inversely proportional to the degree of vegetative cover.

关键词： Soil erosion 137Cs technique Cultivated land Vegetation Xihanshui River Basin

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折叠翼飞行器气动弹性变体飞行仿真平台

北京航空航天大学学报 2024年第6期50卷 1921-1930页

作者：徐浩韩景龙奚勇贾成龙孙钊上海航天控制技术研究所上海201109 南京航空航天大学航空学院南京210016

折叠翼飞行器的变体过程伴随质量分布、刚度分布及气动载荷分布的大幅动态变化,是一个高度复杂的动力学过程。为实现该过程各种动力学问题的有效分析,基于共享内存技术开发了一套高性能的耦合计算程序,并在此基础上整合了Adams多体动力... 详细信息

折叠翼飞行器的变体过程伴随质量分布、刚度分布及气动载荷分布的大幅动态变化,是一个高度复杂的动力学过程。为实现该过程各种动力学问题的有效分析,基于共享内存技术开发了一套高性能的耦合计算程序,并在此基础上整合了Adams多体动力学计算软件、非定常气动力计算程序及飞行控制技术,搭建了折叠翼的气动弹性变体飞行仿真平台。基于该平台,对折叠翼进行了气动弹性分析和飞行变体过程仿真,验证了平台强大的计算和分析能力。

关键词：折叠翼变体飞行器动力学仿真气动弹性飞行仿真

脑血管数字减影血管造影高分辨率分割网络设计

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哈尔滨工程大学学报 2024年第4期45卷 786-793页

作者：崔颖付瑞朱佳高山陈立伟张广哈尔滨工程大学信息与通信工程学院黑龙江哈尔滨150001 哈尔滨医科大学附属第一医院神经外科黑龙江哈尔滨150001

针对现存卷积神经网络对脑血管数字减影血管造影分割精度不高的问题,本文提出了一种基于U-Net的改进网络(IC-Net)。通过融合使用Inception和CAM通道注意力模块,以多种感受域提取更丰富的血管特征信息,并对特征信息进行筛选。增加7×7卷... 详细信息

针对现存卷积神经网络对脑血管数字减影血管造影分割精度不高的问题,本文提出了一种基于U-Net的改进网络(IC-Net)。通过融合使用Inception和CAM通道注意力模块,以多种感受域提取更丰富的血管特征信息,并对特征信息进行筛选。增加7×7卷积层,通过压缩特征层分辨率的方式减少训练过程中产生的数据量。本文所提模型与U-Net、R2U-Net、Attention U-Net相比,IOU、Accuracy、F1-Score和ROC曲线下面积4项指标平均提升了1.82%、0.014%、1.19%和0.73%。结果验证了IC-Net模型明显提升了脑血管数字减影血管造影虚弱血管和血管末端的检测能力,提升了分辨伪影噪声的能力,为医生识别脑血管中产生的病变提供有力参考。

关键词：图像分割特征提取脑血管数字减影血管造影 U-Net Inception模块通道注意力降维处理

Fine mapping and marker-assisted selection (MAS) of a low glutelin content gene in rice

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Cell Research 2005年第8期15卷 622-630页

作者： Yi Hua WANG1, Shi jia LIU1, Su Lan JI1, Wen Wei ZHANG1, Chun Ming WANG1, Ling jiaNG1, jian Min WAN1,2,* 1The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University jiangsu Re- search Center of Plant Engineering, Nanjing 210095, China 2Crop Science Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China The State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University jiangsu Research Center of Plant Engineering jiangsu Research Center of Plant Engineering Nanjing China Crop Science Research Institute Chinese Academy of Agricultural Science Beijing China

Rice with low glutelin content is suitable as functional food for patients affected with diabetes and kidney failure. The fine mapping of the gene(s) responsible for low glutelin content will provide information regarding the distribution of glutelin related genes in rice genome and will generate markers for the selection of low glutelin rice varieties. Following an SDS-PAGE screen of rice germplasm from Taihu Valley of China, Japonica selection W3660 is identified to be a novel mutant characterized with low glutelin content. For fine mapping the mutant gene for low glutelin content, F2 and F3 populations were derived from a cross between W3660 and Jingrennuo. SDS-PAGE analysis of the total endosperm protein showed that the low glutelin content trait was controlled by a single dominant nuclear gene. Genetic mapping, using SSRs, located this gene to chromosome 2, in the region between SSR2-001/SSR2-004 and RM1358. The dis- tances of the two markers to the target gene were 1.1 cM and 3.8 cM respectively. By semi-quantitative RT-PCR analysis, the transcripts of GluB4/GluB5 genes located within the region do not change. However, GluB5 gene located proximal to SSR2-001/SSR2-004 was specifically reduced. SSR profiles of seven Japonica varieties were compared with that of W3660 for loci in the relevant genetic region. The markers SSR2-004 and RM1358 were used for marker- assisted selection. The selection efficiencies of SSR2-004 and RM1358 were 96.8% and 92.7% respectively. This provides a standard starting point for the breeding of low glutelin content rice varieties in China.

关键词： fine mapping low glutelin content marker-assisted selection mutant rice.

Explore the dominant factor in prime editing via a view of DNA processing

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Synthetic and Systems Biotechnology 2023年第3期8卷 371-377页

作者： Zhimeng Xu Dacheng Ma Houzhen Su Xiaodong jia Yinqing Li Yinying lu Zhen Xie MOE Key Laboratory of Bioinformatics and Bioinformatics Division Center for Synthetic and System BiologyDepartment of AutomationBeijing National Research Center for Information Science and TechnologyTsinghua UniversityBeijing100084China Research Center for Biological Computation Zhejiang LaboratoryHangzhou311100China Comprehensive Liver Cancer Centre The Fifth Medical Center of PLA General HospitalBeijingChina School of Pharmaceutical Sciences IDG/McGovern Institute for Brain ResearchTsinghua UniversityBeijingChina

Prime editing is a revolutionary gene-editing method that is capable of introducing insertions,deletions and base substitutions into the ***,the editing efficiency of Prime Editor(PE)is limited by the DNA repair ***,we show that overexpression of the flap structure-specific endonuclease 1(FEN1)and the DNA ligase 1(LIG1)increases the efficiency of prime editing,which is similar to the dominant negative mutL homolog 1(MLH1dn).In addition,MLH1 is still the dominant factor over FEN1 and LIG1 in prime *** results help to further understand the relationship of proteins involved in prime editing and envisage future directions for the development of PE.

关键词： process. prime directions