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Prolonged Consumption of Dietary Advanced Lipoxidation End Products contributes to renal impairment in mice through Dysregulated Intestinal homeostasis

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Food Science and Human Wellness 2024年

作者： Yaya Wang Lu Dong Yaozhong Hu Tianchang Zhang Ruican Wang Linqing nie Junping Wang Yan Zhang Shuo Wang Tianjin Key Laboratory of Food Science and Health， School of Medicine， Nankai University State Key Laboratory of Food Nutrition and Safety， Key Laboratory of Food Nutrition and Safety， College of Food Engineering and Biotechnology， Tianjin University of Science and Technology

Heat processing of food has been well validated as the trigger to generate heat-processing side product of advanced lipoxidation end products (ALEs)， which potentially engenders the threat on systemic health or progression of diseases， especially the accumulated effect after long-term intake. Thus， the study was proposed to evaluate the effect of dietary ALEs on health after long-term ingestion， specifically through simulating the intake of dietary ALE in mice within 9 months to investigate the intervention effect and underlying mechanism. The unexpected observation of renal insufficiency or impairment after long-term intake of dietary ALEs indicated the negative impact on renal health， which has been verified by the pathological analysis. Further studies revealed that a high-ALEs diet disrupted the intestinal barrier， with enhanced impact after disturbing the gut microbiota to potentially lower the abundance of beneficial microbiome through producing nephrotoxic metabolites. Correlation analysis showed that the proliferation of harmful bacteria and the reduction of beneficial bacteria were strongly correlated with intestinal barrier damage and the development of renal insufficiency. Furthermore， the underlying mechanism was unveiled as that ALEs could inhibit AMPK/SIRT1.signaling to fundamentally induce renal inflammation and oxidative stress. Thus， it was revealed that long-term intake of dietary ALE could result in renal impairment， and the results emphasized the control or intervention on dietary ALE to decrease to accumulated impairment on systemic health.

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氟硅缩水甘油醚环氧单体的合成与性能研究

涂料工业 2024年第2期54卷 1-6页

作者：王烁天胡韵朗贾伟聂俊孙芳北京化工大学北京100029 北京化工大学安庆研究院安徽安庆246000

开发可阳离子光聚合的氟硅单体是光聚合领域重要的研究方向。利用易得的原料和简单的工艺合成了一种氟硅缩水甘油醚环氧单体(FSi-GE),采用核磁共振波谱仪(NMR)和傅立叶变换红外光谱仪(FT-IR)对该单体的结构进行了表征,并研究了其对环氧... 详细信息

开发可阳离子光聚合的氟硅单体是光聚合领域重要的研究方向。利用易得的原料和简单的工艺合成了一种氟硅缩水甘油醚环氧单体(FSi-GE),采用核磁共振波谱仪(NMR)和傅立叶变换红外光谱仪(FT-IR)对该单体的结构进行了表征,并研究了其对环氧单体3,4-环氧环己基甲基3,4-环氧环己基甲酸酯(E4221.和1.2-环氧−4-乙烯基环己烷(VOH)组成的阳离子光聚合体系的光聚合动力学、表面疏水性、耐热性及拉伸性能的影响。结果表明:当FSi-GE的添加量从0增加到0.5%,体系的环氧基团最终转化率从60.6%上升到65.7%,固化膜表面水接触角从56.9°升至1.4.6°,断裂伸长率从22.96%升至41.22%,同时耐热性提高。但随着FSi-GE的含量继续增加,表面水接触角、耐热性和断裂伸长率都呈现略微下降的趋势。说明加入适量的FSi-GE能够有效提高E4221.VOH体系的最终转化率、疏水性、耐热性和机械性能。

关键词：阳离子光聚合氟硅单体缩水甘油醚环氧单体

Role of hsa_circ_0007482 in pterygium development: insights into proliferation, apoptosis, and clinical correlations

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International Journal of Ophthalmology(English edition) 2024年第8期17卷 1387-1395页

作者： Li-Yun Zhang Xin Liu Shuo Zheng Wen-Qun Xi Xue-Ping Wu Dan-Yao nie Hui-Ling Hu {1.n-Tao Wang Xin-Hua Liu Jing Zhang Shenzhen Eye Hospital Jinan UniversityShenzhen Eye InstituteShenzhen 518000Guangdong ProvinceChina

AIM:To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts(HPFs)and its correlation with the severity grades of ***:Pterygium and normal conjunctival tissues were collected from the superior area of the same patient’s eye(n=33).The correlation between pterygium severity and hsa_circ_0007482 expression using quantitative reversetranscription polymerase chain reaction(RT-qPCR)were *** distinct siRNA sequences targeting hsa_circ_0007482,along with a negative control sequence,were transfected into *** proliferation was assessed using the cell counting *** levels of Ki67,proliferating cell nuclear antigen(PCNA),Cyclin D1.Bax,B-cell lymphoma-2(Bcl-2),and Caspase-3 were measured via *** staining was employed to detect Ki67 and vimentin *** was evaluated using flow ***:Hsa_circ_0007482 expression was significantly higher in pterygium tissues compared to normal conjunctival tissues(P1..Positive correlations were observed between hsa_circ_0007482 expression and pterygium severity,thickness,and vascular *** of hsa_circ_0007482 inhibited cell proliferation,reducing the mRNA expression of Ki67,PCNA,and Cyclin D1.in ***_circ_0007482 knockdown induced apoptosis,increasing mRNA expression levels of Bax and Caspase-3,while decreasing Bcl-2 expression in ***,hsa_circ_0007482 knockdown attenuated vimentin expression in ***:The downregulation of hsa_circ_0007482 effectively hampers cell proliferation and triggers apoptosis in *** are discernible positive correlations detected between the expression of hsa_circ_0007482 and the severity of pterygium.

关键词： hsa_circ_0007482 circular RNA pterygium cell proliferation apoptosis

UPLC-QQQ-MS/MS指纹图谱结合一测多评法的不同品种苦荞麦质量评价

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中草药 2024年第11期55卷 3855-3865页

作者：王迪杨洁丽聂佳何悦月李维李杰李阳倩成都中医药大学药学院西南特色中药资源国家重点实验室四川成都611137 成都中医药大学资产与实验室管理处四川成都611137 成都大学基础医学院四川成都610106 成都中医药大学本草基因组学研究院四川成都611137

目的建立1.个品种苦荞麦Fagopyrum tataricum指纹图谱,并以芦丁为内标物,采用一测多评法(quantitative analysis of multi-components by single-marker,QAMS)同时检测苦荞麦中葫芦巴碱、表儿茶素、山柰酚-3-O-芸香糖苷、槲皮素、山柰... 详细信息

目的建立1.个品种苦荞麦Fagopyrum tataricum指纹图谱,并以芦丁为内标物,采用一测多评法(quantitative analysis of multi-components by single-marker,QAMS)同时检测苦荞麦中葫芦巴碱、表儿茶素、山柰酚-3-O-芸香糖苷、槲皮素、山柰酚和大黄素含量,以期对不同产地苦荞麦进行质量控制。方法采用超高效液相-三重四级杆液质联用技术(UPLC-QQQ-MS/MS),首次以一级全扫(Full-MS)和多反应监测(multiple reaction monitoring,MRM)2种采集模式相结合的方式建立UPLC-QQQ-MS/MS指纹图谱共有模式,采用夹角余弦法进行相似度评价。以ThermoFisher Accucore^(TM)C_(1.)色谱柱(1.0 mm×3.0 mm,2.6μm);流动相为乙腈-0.1.甲酸水溶液,体积流量0.3 mL/min,柱温30℃,梯度洗脱。以芦丁作为内标物,建立苦荞中其他6种指标成分的相对校正因子(fs/x),从而计算各待测成分的量,并将QAMS计算值与外标法(external standard method,ESM)实测值进行比较,以验证QAMS的准确性和方法适宜性。运用聚类分析、主成分分析、加权分析等化学计量学分析手段建立苦荞麦综合质量优劣评价方法。结果建立了苦荞麦的UPLC-QQQ-MS/MS指纹图谱,在Full-MS扫描模式下标定了1.个共有峰,各品种间相似度在0.399~0.973;同时以MRM扫描模式结合对照品对其中芦丁等7种指标成分进行了峰指认。以芦丁为内标物,与葫芦巴碱、表儿茶素、山柰酚-3-O-芸香糖苷、槲皮素、山柰酚和大黄素6种有效成分的建立的fs/x重现性良好,分别为0.283、1.338、0.654、1.1.2、8.509、0.709。且采用QAMS测定的1.个品种苦荞麦中7种指标成分含量与ESM的实测值之间无显著性差异(P>0.05)。化学计量学分析结果表明,芦丁、山柰酚-3-O-芸香糖苷和山柰酚是影响苦荞麦质量的主要潜在标志物,质量排序相对靠前的苦荞麦产区主要集中在海拔高、光照强、昼夜温差大的中国西南地区,而指纹图谱相似度差异较明显的荞杂2号、综甜2号和通荞1.排在后3位。结论所建立的指纹图谱结合QAMS简便可行,结果准确,化学计量学分析方法全面客观,可为苦荞麦品质评价和质量控制提供参考和依据。

关键词：苦荞麦指纹图谱一测多评一级全扫多反应监测化学计量学芦丁葫芦巴碱表儿茶素山柰酚-3-O-芸香糖苷槲皮素山柰酚大黄素

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含不同取代基的钌-锗化合物的合成及表征

有机化学 2024年第1期44卷 173-179页

作者：田雁董睿聂鹏许波贵州省食品药品检验所贵阳550004

钌-锗化合物(PPh3)(X)RuGeCl_(2)ArTrip(X=H,1.Cl,2;ArTrip=C_(6)H_(3)-2-(η^(6)-Trip)-6-Trip,Trip=2,4,6-i Pr3-C_(6)H_(3))与有机试剂如格氏试剂、LiHBEt3以及萘钠反应实现了锗和钌原子上的取代基团的调控.化合物1.格氏试剂EtMgBr在不同的温度下反应,分别生成(PPh3)HRuGeBr2ArTrip(3)和(PPh3)HRuGeEt2ArTrip(4).化合物1.LiHBEt3反应也生成化合物4,在该反应中LiHBEt3作为乙基转移试剂而不是氢化试剂.此外,化合物1.HBF4反应生成离子型化合物[(PPh_(3))H2RuGeCl_(2)ArTrip][BF4](5).另外,利用Na/naphthalene还原化合物2可以得到双羟基化合物(PPh3)HRuGe(OH)2-ArTrip(6),而化合物2与碘单质的反应历经单碘化,形成化合物(PPh3)IRuGeCl2ArTrip(7).所有的化合物都经过详细的核磁谱学和X射线单晶衍射的表征.

关键词：钌锗宾取代反应结构

Proteomic analysis of ferroptosis pathways reveals a role of CEPT1.in suppressing ferroptosis

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Protein & Cell 2024年第9期15卷 686-703页

作者： Xiaoguang Liu Zhen Chen Yuelong Yan Fereshteh Zandkarimi Litong nie Qidong Li Amber Horbath Kellen Olszewski Lavanya Kondiparthi Chao Mao Hyemin Lee Li Zhuang Masha Poyurovsky Brent RStockwell Junjie Chen Boyi Gan Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer CenterHoustonTX 77030USA Department of Biological Sciences and Department of Chemistry Columbia UniversityNew YorkNY 10027USA Kadmon Corporation LLC(A Sanofi Company)New YorkNY 10016USA The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences HoustonTX 77030USA The Barer Institute PhiladelphiaPA 19104USA

Ferroptosis has been recognized as a unique cell death modality driven by excessive lipid peroxidation and unbalanced cellular *** this study,we established a protein interaction landscape for ferroptosis pathways through proteomic analyses,and identified choline/ethanolamine phosphotransferase 1.CEPT1.as a lysophosphatidylcholine acyltransferase 3(LPCAT3)-interacting protein that regulates LPCAT3 protein *** contrast to its known role in promoting phospholipid synthesis,we showed that CEPT1.suppresses ferroptosis potentially by interacting with phospholipases and breaking down certain pro-ferroptotic polyunsaturated fatty acid(PUFA)-containing ***,our study reveals a previously unrecognized role of CEPT1.in suppressing ferroptosis.

关键词： proteomics ferroptosis CEPT1 LPCAT3

Endoplasmic reticulum-targeted delivery of celastrol and PD-L1.siRNA for reinforcing immunogenic cell death and potentiating cancer immunotherapy

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3643-3660页

作者： Jie Wang Zilong Zhang Yan Zhuo Zhuan Zhang Rongrong Chen Li Liang Xiaohe jiang Di {1. Chang Liu Zhiwen Zou Xiang Li jiaxin Li Bingqi Wang Rui Wang Yong Gan Miaorong Yu School of Pharmacy Shanghai University of Traditional Chinese MedicineShanghai 201203China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China jiangxi Medical College Nanchang UniversityNanchang 330006China School of Pharmacy Nanjing University of Chinese MedicineNanjing 210023China University of Chinese Academy of Sciences Beijing 100049China NMPA Key Laboratory for Quality Research and Evaluation of Pharmaceutical Excipients National Institutes for Food and Drug ControlBeijing 100050China

The prospect of employing chemoimmunotherapy targeted towards the endoplasmic reticulum(ER)presents an opportunity to amplify the synergistic effects of chemotherapy and *** this study,we initially validated celastrol(CEL)as an inducer of immunogenic cell death(ICD)by promoting ER stress and autophagy in colorectal cancer(CRC)***,an ER-targeted strategy was posited,involving the codelivery of CEL with PD-L1.small interfering RNAs(siRNA)using KDEL peptide-modified exosomes derived from milk(KME),to enhance chemoimmunotherapy *** findings demonstrate the efficient transportation of KME to the ER via the Golgi-to-ER *** to their non-targeting counterparts,KME exhibited a significant augmentation of the CEL-induced ICD ***,it facilitated the release of danger signaling molecules(DAMPs),thereby stimulating the antigen-presenting function of dendritic cells and promoting the infiltration of T cells into the ***,the ER-targeted delivery of PD-L1.siRNA resulted in the downregulation of both intracellular and membrane PD-L1.protein expression,consequently fostering the proliferation and activity of CD8^(+)T ***,the ER-targeted formulation exhibited enhanced anti-tumor efficacy and provoked anti-tumor immune responses against orthotopic colorectal tumors in ***,a robust ER-targeted delivery strategy provides an encouraging approach for achieving potent cancer chemoimmunotherapy.

关键词： Chemoimmunotherapy Targeted drug delivery Endoplasmic reticulum Endoplasmic reticulum stress Immunogenic cell death siRNA Chemoimmunotherapy Targeted drug delivery Endoplasmic reticulum Endoplasmic reticulum stress Immunogenic cell death siRNA

Diameter-dependent ultra-high thermoelectric performance of ZnO nanowires

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Chinese Physics B 2024年第4期33卷 86-94页

作者：聂祎楠唐桂华李一斐张敏赵欣 MOE Key Laboratory of Thermo-Fluid Science and Engineering School of Energy and Power EngineeringXi’an Jiaotong UniversityXi’an 710049China

Zinc oxide(ZnO)shows great potential in electronics,but its large intrinsic thermal conductivity limits its thermoelectric *** this work,we explore the significant carrier transport capacity and diameter-dependent thermoelectric characteristics of wurtzite-ZnO(0001.nanowires based on first-principles and molecular dynamics *** the synergistic effect of band degeneracy and weak phonon-electron scattering,P-type(ZnO)_(73) nanowires achieve an ultrahigh power factor above 1.00μW·cm^(-1.·K^(-2)over a wide temperature *** lattice thermal conductivity and carrier transport properties of ZnO nanowires exhibit a strong diameter size *** the ZnO nanowire diameter exceeds 1..72A,the carrier transport properties increase significantly,while the thermal conductivity shows a slight increase with the diameter size,resulting in a ZT value of up to 6.4 at 700 K for P-type(ZnO)_(73).For the first time,the size effect is also illustrated by introducing two geometrical configurations of the ZnO *** work theoretically depicts the size optimization strategy for the thermoelectric conversion of ZnO nanowires.

关键词： ZnO nanowire size effect thermoelectric performance deformation potential theory

Identification of SSR markers linked to the abscission of cotton boll traits and mining germplasm in Cotton

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Journal of Cotton Research 2024年第2期7卷 177-187页

作者： SHUI Guangling LIN Hairong MA Xiaomei ZHU Bo HAN Peng AINI Nurimanguli GUO Chunping WU Yuanlong PAN Zhenyuan YOU Chunyuan SONG Guoli nie Xinhui Key Laboratory of Oasis Ecology Agricultural of Xinjiang Bingtuan Agricultural CollegeShihezi UniversityShiheziXinjiang 832003China Cotton Institute Xinjiang Academy of Agriculture and Reclamation ScienceShiheziXinjiang 832000China Agricultural Science Research Institute of the 5th Division of Xinjiang Production and Construction Corps ShuangheXinjiang 833408China Modern Agricultural College Kashi UniversityKashiXinjiang 844000China National Key Laboratory of Crop Genetic Improvement College of Plant Sciences&TechnologyHuazhong Agricultural UniversityWuhanHubei 430070China National Key Laboratory of Cotton Bio‑Breeding and Integrated Utilization Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000China

Background Cotton is an economically important *** is crucial to find an effective method to improve cotton yield,and one approach is to decrease the abscission of cotton bolls and ***,the lack of knowledge of the genetic and molecular mechanisms underlying cotton boll abscission traits has hindered genetic *** Pearson’s correlation analysis revealed a significant positive correlation between boll abscission rates 1.AR1.and boll abscission rates 2(AR2).A genome-wide association study was conducted on 1.5 loci that exhibited high polymorphism and were uniformly distributed across 26 chromosomes(pair).The study revealed 1.,46,and 62 markers that were significantly associated with boll abscission,fiber quality,and yield traits(P1.75%–7.1.%,1.1.%–9.58%,and 1.40%–5.44%of the phenotypic variation,***,the marker MON_SHIN-1.84b was associated with the cotton boll abscission trait,whereas MON_CGR5732a was associated with cotton boll abscission and fiber quality *** of the marker loci identified in this study had been previously *** on phenotypic effects,six typical cultivars with elite alleles related to cotton boll abscission,fiber quality,and yield traits were *** cultivars hold great promise for widespread utilization in breeding *** These results lay the foundation for understanding the molecular regulatory mechanism of cotton boll abscission and provide data for the future improvement of cotton breeding.

关键词： SSR Genome wide association studies Abscission Favorable alleles Cotton Genetic improvement