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Expression and function of classical protein kinase C isoenzymes in gastric cancer cell line and its drugresistant sublines

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World Journal of Gastroenterology 2002年第3期8卷 441-445页

作者： Ying Han Zhe-Yi Han Xin-Min Zhou Ru Shi Yue Zheng Yong-Quan Shi ji-yan miao Bo-Rong Pan Dai-Ming Fan Institute of Digestive Disease Xijing HospitalFourth Military Medical UniversityXi’an 710032Shannxi ProvinceChina Department of Pharmaeology and Reagents Xijing HospitalFourth Military Medical UniversityXi’an 710032Shannxi ProvinceChina the 6th Undergraduate Section Fourth Military Medical UniversityXi’an 710032Shannxi ProvinceChina Oncology Center of Xijing Hospital Fourth Military Medical UniversityXi’an 710032Shannxi ProvinceChina

AIM: To investigate the expression and function of classicalprotein kinase C (PKC) isoenzymes in inducing MDRphenotype in gastric cancer ***: Two cell lines were used in the study: gastriccancer cell SGC7901 and its drug-resistant cell SGC7901/VCRstepwise-selected by vincristine 0.3, 0. 7 and 1.0 mg@ L-1 ,respectively. The expression of classical PKC (cPKC)isoenzymes in SGC7901 cells and SGC7901/VCR cells weredetected using immunofluorescent cytochemistry, laserconfocal scanning microscope and Wsstern blot. The effectsof anti-PKC isoenzymes antibody of adriamycinaccumulation in SGC7901/VCR cells were determined usingflow cytometric ***: (1) SGC7901 cells exhibited positive staining ofPKC-α. SGC7901/VCR cells exhibited stronger staining ofPKC-α than SGC7901 cells. The higher dosage vincristineselected, the much stronger staining of PKC-α was observedon SGC7901/VCR cells. (2) Both SGC7901 and SGC7901/VCRcells exhibited positive staining of PKC-βⅠ and PKC-βⅡ withno significant difference. ( 3 ) Compared with SGC7901,SGC7901/VCR cells had decreased adriamycin accumulationand retention. Accumulation of adriamycin in SGC7901 was5.21 + 2.56 mg@ L-1, in SGC7901/VCR 0.3 was 0.85 + 0.29 mg@L-1 , in SGC7901/VCR 0.7 was 0.81 + 0.32 og@ L-1 , and inSGC7901NCR 1.0 was 0.80 + 0.33 mg @ L-1; Retention ofadriamycin in SGC 7901 was 2.51 + 1.23 mg@L-1, in SGC7901/VCR 0.3 was 0.47 + 0.14 mg@ L-1 , in SGC7901/VCR 0.7 was 0.44 + 0.15 mg@ L-1, and in SGC 7901/VCR 1.0 was 0.41 + 0.1 1mg @ L-1 . (4) Fluorescence intensity presented adriamycinaccumulation in SGC7901/VCR cells was increased from 1.14+0.36 to 2.71 +0.94 when cells were co-incubated with anti-PKC-αbut not with anti-PKC-βⅠ, PKC-βⅡ and PKCγ ***: PKC-α, but not PKC-βⅠ, PKC-βⅡ or PKCγ,may play a role in multidrug resistance of gastric cancercells SGC7901/VCR.

关键词： C蛋白激酶同工酶胃癌细胞表达抗药性

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基于语料库的译者风格与翻译策略研究——以《红楼梦》中报道动词及英译为例

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解放军外国语学院学报 2010年第4期33卷 87-92页

作者：刘泽权闫继苗燕山大学外国语学院河北秦皇岛066004 燕山大学里仁学院河北秦皇岛066004

本文旨在对《红楼梦》英文语料库中前56回的报道动词及3个英译本的翻译进行描述性研究,通过"道"的最频繁报道形式"(某人)道"的翻译,考察3位译者的翻译风格和策略。结果表明,译者对报道动词的选择受报道方式和话语内容等多方面影响,数据... 详细信息

本文旨在对《红楼梦》英文语料库中前56回的报道动词及3个英译本的翻译进行描述性研究,通过"道"的最频繁报道形式"(某人)道"的翻译,考察3位译者的翻译风格和策略。结果表明,译者对报道动词的选择受报道方式和话语内容等多方面影响,数据统计结果印证了报道动词反映译者风格、翻译策略呈显化趋势的假设。

关键词：红楼梦翻译策略语料库报道动词道

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我国不同时代流行爱情歌曲的意识形态倾向

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燕山大学学报（哲学社会科学版） 2007年第1期8卷 95-101页

作者：刘泽权闫继苗燕山大学外国语学院河北秦皇岛066004

流行爱情歌曲的演变是意识形态的缩影。分析不同年代流行爱情歌曲中的意识形态倾向,比较不同年代歌曲中承载的隐性意识形态因素,可以揭示歌词内容与特定时期意识形态相互作用的密切关系。

关键词：流行爱情歌曲意识形态歌词价值观

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Emerging structures and dynamic mechanisms ofγ-secretase for Alzheimer’s disease

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Neural Regeneration Research 2025年第1期20卷 174-180页

作者： Yinglong miao Michael S.Wolfe Computational Medicine Program and Department of Pharmacology University of North Carolina–Chapel HillChapel HillNCUSA Department of Medicinal Chemistry University of KansasLawrenceKSUSA

γ-Secretase,called“the proteasome of the membrane,”is a membrane-embedded protease complex that cleaves 150+peptide substrates with central roles in biology and medicine,including amyloid precursor protein and the Notch family of cell-surface *** inγ-secretase and amyloid precursor protein lead to early-onset familial Alzheimer’s disease.γ-Secretase has thus served as a critical drug target for treating familial Alzheimer’s disease and the more common late-onset Alzheimer’s disease as ***,critical gaps remain in understanding the mechanisms of processive proteolysis of substrates,the effects of familial Alzheimer’s disease mutations,and allosteric modulation of substrate cleavage byγ-*** this review,we focus on recent studies of structural dynamic mechanisms ofγ-*** mechanisms,including the“Fit-Stay-Trim,”“Sliding-Unwinding,”and“Tilting-Unwinding,”have been proposed for substrate proteolysis of amyloid precursor protein byγ-secretase based on all-atom molecular dynamics *** an incorrect registry of the Notch1 substrate was identified in the cryo-electron microscopy structure of Notch1-boundγ-secretase,molecular dynamics simulations on a resolved model of Notch1-boundγ-secretase that was reconstructed using the amyloid precursor protein-boundγ-secretase as a template successfully capturedγ-secretase activation for proper cleavages of both wildtype and mutant Notch,being consistent with biochemical experimental *** approach could be potentially applied to decipher the processing mechanisms of various substrates byγ-*** addition,controversy over the effects of familial Alzheimer’s disease mutations,particularly the issue of whether they stabilize or destabilizeγ-secretase-substrate complexes,is ***,an outlook is provided for future studies ofγ-secretase,including pathways of substrate binding and product release,effects of modulators on familial Alzheimer’s disease mutations

关键词： Alzheimer’s disease amyloid precursor protein cryo-EM structures drug design intramembrane proteolysis molecular dynamics Notch

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Repetitive transcranial magnetic stimulation in Alzheimer’s disease:effects on neural and synaptic rehabilitation

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Neural Regeneration Research 2025年第2期20卷 326-342页

作者： Yi ji Chaoyi yang Xuerui Pang Yibing yan Yue Wu Zhi Geng Wenjie Hu Panpan Hu Xingqi Wu Kai Wang Department of Neurology The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui ProvinceChina Anhui Province Key Laboratory of Cognition and Neuropsychiatric Disorders HefeiAnhui ProvinceChina Collaborative Innovation Center of Neuropsychiatric Disorders and Mental Health HefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina Department of Psychology and Sleep Medicine The Second Affiliated Hospital of Anhui Medical UniversityHefeiAnhui ProvinceChina

Alzheimer’s disease is a neurodegenerative disease resulting from deficits in synaptic transmission and *** Alzheimer’s disease brain tends to be hyperexcitable and hypersynchronized,thereby causing neurodegeneration and ultimately disrupting the operational abilities in daily life,leaving patients *** transcranial magnetic stimulation is a cost-effective,neuro-modulatory technique used for multiple neurological *** the past two decades,it has been widely used to predict cognitive decline;identify pathophysiological markers;promote neuroplasticity;and assess brain excitability,plasticity,and *** has also been applied to patients with dementia,because it can yield facilitatory effects on cognition and promote brain recovery after a neurological ***,its therapeutic effectiveness at the molecular and synaptic levels has not been elucidated because of a limited number of *** study aimed to characterize the neurobiological changes following repetitive transcranial magnetic stimulation treatment,evaluate its effects on synaptic plasticity,and identify the associated *** review essentially focuses on changes in the pathology,amyloidogenesis,and clearance pathways,given that amyloid deposition is a major hypothesis in the pathogenesis of Alzheimer’s *** mechanisms associated with repetitive transcranial magnetic stimulation procedures and different pathways mediating gene transcription,which are closely related to the neural regeneration process,are also ***,we discuss the outcomes of animal studies in which neuroplasticity is modulated and assessed at the structural and functional levels by using repetitive transcranial magnetic stimulation,with the aim to highlight future directions for better clinical translations.

关键词： Alzheimer’s disease amyloid deposition apoptotic mechanisms biomarker neural regeneration neurodegeneration repetitive transcranial magnetic stimulation synaptic plasticity

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Inspires effective alternatives to backpropagation:predictive coding helps understand and build learning

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Neural Regeneration Research 2025年第11期20卷 3215-3216页

作者： Zhenghua Xu miao Yu Yuhang Song School of Health Sciences and Biomedical Engineering Hebei University of TechnologyTianjinChina Department of Computer Science University of OxfordOxfordUK

Artificial neural networks are capable of machine learning by simulating the hiera rchical structure of the human *** enable learning by brain and machine,it is essential to accurately identify and correct the prediction errors,referred to as credit assignment(Lillicrap et al.,2020).It is critical to develop artificial intelligence by understanding how the brain deals with credit assignment in neuroscience.

关键词： assignment learning enable

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Nanomaterials-mediated lysosomal regulation:a robust protein-clearance approach for the treatment of Alzheimer’s disease

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Neural Regeneration Research 2025年第2期20卷 424-439页

作者： Mengqi Hao jianjian Chu Tinglin Zhang Tong Yin Yuankai Gu Wendanqi Liang Wenbo ji jianhua Zhuang yan Liu jie Gao You Yin Department of Neurology Second Affiliated Hospital of Naval Medical University(Shanghai Changzheng Hospital)ShanghaiChina School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghaiChina Changhai Clinical Research Unit Shanghai Changhai HospitalNaval Medical UniversityShanghaiChina Department of Clinical Pharmacy Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of Neurology Shanghai East HospitalSchool of MedicineTongji UniversityShanghaiChina

Alzheimer’s disease is a debilitating,progressive neurodegenerative disorder characterized by the progressive accumulation of abnormal proteins,including amyloid plaques and intracellular tau tangles,primarily within the ***,crucial intracellular organelles responsible for protein degradation,play a key role in maintaining cellular *** studies have suggested a link between the dysregulation of the lysosomal system and pathogenesis of neurodegenerative diseases,including Alzheimer’s *** the normal physiological function of lysosomes hold the potential to reduce the pathological burden and improve the symptoms of Alzheimer’s ***,the efficacy of drugs in treating Alzheimer’s disease is limited,with major challenges in drug delivery efficiency and ***,nanomaterials have gained widespread use in Alzheimer’s disease drug research owing to their favorable physical and chemical *** review aims to provide a comprehensive overview of recent advances in using nanomaterials(polymeric nanomaterials,nanoemulsions,and carbon-based nanomaterials)to enhance lysosomal function in treating Alzheimer’s *** review also explores new concepts and potential therapeutic strategies for Alzheimer’s disease through the integration of nanomaterials and modulation of lysosomal *** conclusion,this review emphasizes the potential of nanomaterials in modulating lysosomal function to improve the pathological features of Alzheimer’s *** application of nanotechnology to the development of Alzheimer’s disease drugs brings new ideas and approaches for future treatment of this disease.

关键词： Alzheimer’s disease autophagy dysfunction lysosomal acidification lysosomal system nanomaterials neurodegenerative diseases

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Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 39-48页

作者： Peng Li Meifeng Cai Shengjun miao Yuan Li Yu Wang Key Laboratory of Ministry of Education for Efficient Mining and Safety of Metal Mines University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Coal Resources and Safe Mining China University of Mining and TechnologyXuzhou 221116China

Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress *** investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution *** results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with *** is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and ***,there is no obvious relationship between the rock quality designation(RQD)and *** maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same *** addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data.

关键词： overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification

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GOMSS: A Simple Group Oriented(t, m, n) Multi-secret Sharing Scheme

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Chinese Journal of Electronics 2017年第3期26卷 557-563页

作者： miao Fuyou WANG Li ji yangyang XIONG yan School of Computer Science and Technology University of Science and Technology of China

In most(t,n)-Multi-secret sharing((t,n)-MSS)schemes,an illegal participant,even without any valid share,may recover secrets when there are over t participants in secret *** address this problem,the paper presents the notion of Group oriented(t,m,n)-multi-secret sharing(or(t,m,n)-GOMSS),in which recovering each secret requires all m(n≥m≥t)participants to have valid shares and actually participate in secret *** an example,the paper then proposes a simple(t,m,n)-GOMSS *** the scheme,every shareholder has only one share;to recover a secret,m shareholders construct a Polynomial-based randomized component(PRC)each with the share to form a tightly coupled group,which forces the secret to be recovered only with all m valid *** a result,the scheme can thwart the above illegal participant *** scheme is simple as well as flexible and does not depend on conventional hard problems or one way functions.

关键词： Multi-secret sharing Shamirs scheme Tightly coupled group Polynomial-based randomized component(PRC)

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Asynchronous Group Authentication

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Chinese Journal of Electronics 2017年第4期26卷 820-826页

作者： miao Fuyou jiANG Huiwen ji yangyang XIONG yan School of Computer Science and Technology University of Science and Technology of China

Group authentication usually checks whether an individual user belongs to a pre-defined group each time but cannot authenticate all users at once without public key system. The paper proposes a Randomized component-based asynchronous(t, m, n) group authentication((t, m, n)-RCAGA) scheme. In the scheme, each user employs the share of(t, n)-threshold secret sharing as the token, constructs a Randomized component(RC) with the share and verifies whether all users belong to a pre-defined group at once without requiring all users to release randomized components simultaneously. The proposed scheme is simple and flexible because each group member just uses a single share as the token and the scheme does not depend on any public key system. Analyses show the proposed scheme can resist up to t-1 group members conspiring to forge a token, and an adversary is unable to forge a valid token or derive a token from a RC.

关键词： Randomized component(RC) Group au thentication Chinese remainder theorem Asynchronous

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