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Rapid Inactivation of Chloroplastic Ascorbate Peroxidase is Responsible for Oxidative Modification to Rubisco in Tomato (Lycopersicon esculentum) under Cadmium Stress

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Journal of Integrative Plant Biology 2008年第4期50卷 415-426页

作者： Kai-Lang Liu Lin Shen jia-Qi Wang ji-ping sheng College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100094 China

To investigate the sensitive site of antioxidant systems in chloroplast under cadmium stress and its consequence on reactive oxygen species production and action, the sub-organellar localization of chloroplast superoxide dismutases （SOD, EC 1.***.1.1） and ascorbic peroxidase （APX, EC 1.***.1.11） isoenzymes and changes of enzymes activities under cadmium stress were investigated in tomato seedlings. Two APX isoforms, one thylakoid-bound and one stromal, were detected. Cd at 50 μM induced a moderate increase of SOD activities but a rapid inactivation of both APX isoenzymes. APX inactivation was mainly related to the decrease of ascorbate concentration, as supported by in vitro treatment of exogenous ascorbate and APX kinetic properties under Cd stress. H2O2 accumulation in chloroplast, as a consequence of APX inactivation, was associated with a 60% loss of Rubisco （EC 4.***.1.39） activity, which could be partially accounted for by a 10% loss of Rubisco content. Protein oxidation assay found that the Rubisco large subunit was the most prominent carbonylated protein; the level of carbonylated Rubisco large subunit increased fivefold after Cd exposure. Thiol groups in the Rubisco large subunit were oxidized, as indicated by non-reducing electrophoresis. Treating crude extract with H2O2 resulted in a similar pattern of protein oxidation and thiols oxidation with that observed in Cd-treated plants. Our study indicates that APXs in the chloroplast is a highly sensitive site of antioxidant systems under Cd stress, and the inactivation of APX could be mainly responsible for oxidative modification to Rubisco and subsequent decrease in its activity.

关键词： ascorbate peroxidase cadmium hydrogen peroxide protein oxidation Rubisco.

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Two novel gene-specific markers at the Pik locus facilitate the application of rice blast resistant alleles in breeding

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Journal of Integrative Agriculture 2021年第6期20卷 1554-1562页

作者： TIAN Da-gang CHEN Zi-qiang LIN Yan CHEN Zai-jie LUO jia-mi ji ping-sheng YANG Li-ming WANG Zong-hua WANG Feng Fujian Key Laboratory of Genetic Engineering for Agriculture/Biotechnology Research Institute Fujian Academy of Agricultural SciencesFuzhou 350003P.R.China State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops College of Life ScienceFujian Agriculture and Forestry UniversityFuzhou 350002P.R.China College of Biology and the Environment Nanjing Forestry UniversityNanjing 210037P.R.China Department of Plant Pathology University of GeorgiaTiftonGA 31793USA

Blast,a disease caused by Magnaporthe oryzae,is a major constraint for rice production *** of durable blast resistance genes into high-yielding rice cultivars has been considered a priority to control the *** blast resistance Pik locus,located on chromosome 11,contains at least six important resistance genes,but these genes have not been widely employed in resistance breeding since existing markers hardly satisfy current breeding needs due to their limited scope of *** this study,two PCR-based markers,Pikp-Del and Pi1-In,were developed to target the specific In Del(insertion/deletion)of the Pik-p and Pi-1 genes,*** two markers precisely distinguished Pik-p,Pi-1,and the K-type alleles at the Pik locus,which is a necessary element for functional genes from rice *** also revealed that only several old varieties contain the two genes,of which nearly half carry the K-type ***,these identified varieties can serve as new gene sources for developing blast resistant *** two newly developed markers will be highly useful for the use of Pik-p,Pi-1 and other resistance genes at the Pik locus in markerassisted selection(MAS)breeding programs.

关键词： rice blast disease molecular marker Pik-p Pi-1 K-type alleles

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Long-term effectiveness of moxibustion on herbs for moderate-severe persistent allergic rhinitis population:A study protocol for a randomized controlled trial

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World Journal of Acupuncture-Moxibustion 2018年第3期28卷 212-217,I0007页

作者： Yi-fan jiA ji-ping ZHAO,sheng-nan GUO Qi XIE Yi XIAO sheng CHEN Dongzhimen Hospital Beijing University of Chinese Medicine Belting 100700 China China Academy of Chinese Medical Sciences Institute of Acupuncture and Moxibustion Beijing 100700 China

Background： Allergic rhinitis（AR） is defined as an immunoglobulin led atopic disorder that affects the nasal mucosa. Moxibustion on herbs, a common complementary and alternative medicine approach, is frequently used for treating AR in clinical practice. Western medicine is good at quick symptomatic relief,while offer little or no sustainable and steady long-term effect. Little established evidence is available to support the long-term effectiveness of moxibustion on herbs for ***： This study is a randomized controlled trial to assess the long-term effectiveness of moxibustion on herbs in moderate-severe persistent AR ***： The study sample size is 56 patients. Eligible patients with moderate-severe persistent AR will be randomized into a moxibustion on herbs combined with conventional treatment group（MOHCT group） and a conventional treatment group（CT group） in a 1：1 ratio. Patients in the MOHCT group will receive a 30-min moxibustion on herbs treatment on Zhiyang（至阳 GV 9）, Dàzhuī（大椎 GV 14） and bilateral Shenshu（肾俞 BL 23）, Fèishū（肺俞 BL 13） for a total of 12 times on the basis of conventional treatment, while those in the CT group will receive conventional treatment alone. The primary outcome measure is VAS score for total nasal symptoms, which will be obtained via a self-recorded AR diary. The secondary outcome measures include the average occurrence of symptoms per week, use of medication and Rhinoconjunctivitis Quality of Life Questionnaire（RQLQ）.Discussion： The objectives of this study include（1） to evaluate the long-term effectiveness and safety of moxibustion on herbs for treating AR;（2） to evaluate whether moxibustion on herbs can reduce the frequency of AR symptoms in patients with moderate-severe persistent allergic rhinitis. The finding of this study will provide evidence on the long-term effectiveness of moxibustion on herbs for moderatesevere persistent AR.

关键词： Moxibustion on herbs Moderate-severe persistent Allergic rhinitis Long-term effectiveness Randomized controlled trial (RCT) Protocol

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莘县甜瓜产业发展现状与思考

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园艺与种苗 2023年第2期43卷 68-70页

作者：姬生平苗纪忠莘县农业农村局山东莘县252400

莘县是全国最大的早春设施甜瓜种植基地。该文从种植面积和品种、设施类型、栽培技术、销售方式几个方面介绍了莘县甜瓜产业发展现状,对目前甜瓜生产和销售中存在的问题进行了分析,在此基础上对莘县甜瓜产业今后的发展方向进行了总结。

关键词：莘县甜瓜发展现状存在问题发展方向

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一种PSK接口电路的容差设计研究

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电子测量技术 2016年第12期39卷 31-37页

作者：计平李晟董方成中国空间技术研究院通信卫星事业部北京100094 中国空间技术研究院北京100094 中国空间技术研究院总体部北京100094

介绍了电路容差设计、分析和分配的定义,并提出了一种基于EDA仿真技术的电路容差设计方法。该容差设计方法综合运用了参数扫描分析、灵敏度分析和最坏情况分析等多种容差分析手段,可以对电路中影响电路容差性能的关键参数进行仿真分析... 详细信息

介绍了电路容差设计、分析和分配的定义,并提出了一种基于EDA仿真技术的电路容差设计方法。该容差设计方法综合运用了参数扫描分析、灵敏度分析和最坏情况分析等多种容差分析手段,可以对电路中影响电路容差性能的关键参数进行仿真分析和确认。利用Saber软件将该容差设计方法应用在PSK接口电路中,根据容差设计步骤,通过调整电路组成元器件的参数标称值,并通过为各元器件分配公差,提高了该电路输出特性的容差性能,为提高航天电子产品的可靠性水平提供了一种新的途径。

关键词：容差设计容差分析 EDA PSK

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马斯京根方程参数的确定及其程序化

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水科学与工程技术 2005年第2期 11-13页

作者：张洪国张洪文汲生平泛华工程有限公司岩土部北京100055 山东省莒南县水利局山东莒南276600 山东省莒南县建设局山东莒南276600

常用的试算法在确定马斯京根方程参数时一般得不到最优结果,且计算较繁杂。针对这一缺陷,尝试应用搜索法来确定其参数,并将方法编成计算机程序,使之具有通用性,实现了程序化。在洪水预报实例应用中,该方法所确定的参数值较试算法更优,... 详细信息

常用的试算法在确定马斯京根方程参数时一般得不到最优结果,且计算较繁杂。针对这一缺陷,尝试应用搜索法来确定其参数,并将方法编成计算机程序,使之具有通用性,实现了程序化。在洪水预报实例应用中,该方法所确定的参数值较试算法更优,也更简便,从而实现了求参数过程的优化和简化。

关键词：马斯京根方程搜索法程序化参数确定流量过程

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Unraveling the mechanism of action of Shangxia Liangji formula for treating insomnia:a metabolomics and network pharmacology approach

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Traditional Medicine Research 2025年第2期10卷 16-29页

作者： Xia-jie Quan Hao Liang Yong-Hong Tang Li jiang Xiong-Ying ji Feng-Ying Zhang ping Zhang Bo Ouyang Department of Information The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Neurology The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Gastroscopy The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Traditional Chinese Medicine The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China

Background:Insomnia is a prevalent clinical condition and Shangxia Liangji formula(SXLJF)is a well-established method of ***,the specific mechanism of action of SXLJF remains ***:The mouse model of insomnia was established by intraperitoneal injection of ***-two mice were randomly divided into a negative control group,model group,SXLJF group(18.72 g/kg/day),and positive control group(diazepam,2 mg/kg)and treated with the corresponding drugs for 7 consecutive *** open field test and pentobarbital-induced sleeping test were ***-MS-based untargeted metabolomics and network pharmacology were applied to explore the potential targets of SXLJF for treating ***,key targets were validated using ***:Behavioral tests demonstrated that SXLJF reduced the total distance,average velocity,central distance,and sleep latency,and prolonged sleep *** and network pharmacology revealed potential targets,signaling pathways,metabolic pathways,and metabolites associated with the anti-insomnia effects of ***,tyrosine hydroxylase(TH)and tyrosine metabolism emerged as crucial metabolic pathways and targets,***-qPCR results supported the role of TH in the mechanism of SXLJF in treating ***:In conclusion,TH and tyrosine metabolism may represent significant targets and pathways for SXLJF in treating insomnia.

关键词： Shangxia Liangji formula insomnia metabolomics network pharmacology tyrosine hydroxylase tyrosine metabolism

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Investigation of polycyclic aromatic hydrocarbons from coal gasification

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Journal of Environmental Sciences 2005年第1期17卷 141-145页

作者： ZHOUHong-cang jiNBao-sheng ZHONGZhao-ping HUANGYa-ji XIAORui LIDa-ji KeyLaboratoryofCleanCoalPowerGenerationandCombustionTechnologyofMinistryofEducation ThermoenergyEngineeringResearchInstituteSoutheastUniversityNanjing210096China

The hazardous organic pollutants generated from coal gasification, such as polycyclic aromatic hydrocarbons(PAHs), are highly mutagenic and carcinogenic. More researchers have paid particular attention to them. Using air and steam as gasification medium, the experiments of three kinds of coals were carried out in a bench scale atmospheric fluidized bed gasifier. The contents of the 16 PAHs specified by US EPA in raw coal, slag, bag house coke, cyclone coke and gas were measured by HPLC to study the contents of PAHs in raw coal and the effects of the inherent characters of coals on the formation and release of PAHs in coal gasification. The experimental results showed that the distributions of PAHs in the gasified products are similar to raw coals and the total\|PAHs content in coal gasification is higher than in raw coal(except Coal C). The total\|PAHs contents increase and then decrease with the rise of fixed carbon and sulfur of coal while there has an opposite variation when volatile matters content increase. The quantities of PAHs reduce with the increase of ash content or the drop of heating value during coal gasification.

关键词： polycyclic aromatic hydrocarbons (PAHs) coal gasification fluidized bed coal rank

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小波分析及其应用

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微机发展 2003年第8期13卷 93-94,100页

作者：马秀红曹继平董晟飞第二炮兵工程学院陕西西安710025

小波分析是传统傅里叶分析发展史上里程碑式的发展,近年来成为众多学科共同关注的热点。文章在小波变换的基础上,介绍了小波变换的一种快速算法—Mallat算法。在此基础上将其应用于信号分析与处理,对故障信号进行分解与重构,通过分析实... 详细信息

小波分析是传统傅里叶分析发展史上里程碑式的发展,近年来成为众多学科共同关注的热点。文章在小波变换的基础上,介绍了小波变换的一种快速算法—Mallat算法。在此基础上将其应用于信号分析与处理,对故障信号进行分解与重构,通过分析实验结果可知,高频部分能够清晰地反映信号的转折点,而这些点正是故障诊断所需的重要信息,通过对这些信息的分析可以得出故障点的位置。因此,可以推断小波分析在信号的奇异性位置检测方面是有效的。

关键词：小波分析信号分析信号处理 Mallat算法小波变换

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The impact of turbulence on electron heating and acceleration near the neutral point of externally driven reconnecting current sheets in solar flares

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Research in Astronomy and Astrophysics 2012年第12期12卷 1701-1713页

作者： Gui-ping Wu Hai-sheng ji Zong-Jun Ning Department of Physics Southeast University Nanjing 210096 China Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210008 China

We aim to investigate the influence of plasma instability on electron acceleration and heating near the neutral point of a turbulent reconnecting current sheet （RCS）.Through numerically solving the one dimensional relativistic Vlasov equation with typical solar coronal parameters and a realistic mass ratio in the presence of a strong inductive electric field E0,we suggest that the wave-particle scattering may produce a flat electron flux spectrum from thermal to nonthermal electrons without a sudden low-energy cutoff in the acceleration *** ratio between electron heating and acceleration decreases with the increase of the induced electric *** is about one for E0=1 V cm-1 and one fourth for E0=10 V *** unstable waves excited by the beam plasma instability first accelerate the electrons,then trap these electrons from further acceleration by an induced electric field through wave-particle resonant interactions.

关键词： turbulence-scattering-Sun:X-rays gamma rays-acceleration of particles

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