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Modulation of stem cell fate in intestinal homeostasis,injury and repair

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World Journal of Stem Cells 2023年第5期15卷 354-368页

作者： Zhe Wang Yan-ji qu Min Cui Department of Gastroenterology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina Department of Otorhinolaryngology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina Department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina

The mammalian intestinal epithelium constitutes the largest barrier against the external environment and makes flexible responses to various types of *** cells are fast-renewed to counteract constant damage and disrupted barrier function to maintain their *** homeostatic repair and regeneration of the intestinal epithelium are governed by the Lgr5+intestinal stem cells(ISCs)located at the base of crypts,which fuel rapid renewal and give rise to the different epithelial cell *** biological and physicochemical stress may challenge epithelial integrity and the function of *** field of ISCs is thus of interest for complete mucosal healing,given its relevance to diseases of intestinal injury and inflammation such as inflammatory bowel ***,we review the current understanding of the signals and mechanisms that control homeostasis and regeneration of the intestinal *** focus on recent insights into the intrinsic and extrinsic elements involved in the process of intestinal homeostasis,injury,and repair,which fine-tune the balance between self-renewal and cell fate specification in *** the regulatory machinery that modulates stem cell fate would aid in the development of novel therapeutics that facilitate mucosal healing and restore epithelial barrier function.

关键词： Intestinal stem cell Epithelial repair Homeostasis Regeneration Selfrenewal Apoptosis

Effects of Kupffer cell inactivation on graft survival and liver regeneration after partial liver transplantation in rats

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Hepatobiliary & Pancreatic Diseases International 2015年第1期14卷 56-62页

作者： Hang-Yu Luo Shan-Fang Ma ji-Fu qu De-Hu Tian Department of Emergency Medicine Beijing Ditan Hospital Capital Medical University Department of Emergency Medicine Daping HospitalThird Military Medical University Orthopetics Department Third Hospital of Hebei Medical University

BACKGROUND： Gadolinium chloride（Gd Cl3） selectively inactivates Kupffer cells and protects against ischemia/reperfusion and endotoxin injury. However, the effect of Kupffer cell inactivation on liver regeneration after partial liver transplantation（PLTx） is not clear. This study was to investigate the role of Gd Cl3 pretreatment in graft function after PLTx, and to explore the potential mechanism involved in this ***： PLTx（30% partial liver transplantation） was performed using Kamada’s cuff technique, without hepatic artery reconstruction. Rats were randomly divided into the control low-dose（5 mg/kg） and high-dose（10 mg/kg） Gd Cl3 groups. Liver injury was determined by the plasma levels of alanine aminotransferase and aspartate aminotransferase, liver regeneration by PCNA staining and Brd U uptake, apoptosis by TUNEL assay. IL-6 and p-STAT3 levels were measured by ELISA and Western ***： Gd Cl3 depleted Kupffer cells and decreased animal survival rates, but did not significantly affect alanine aminotransferase and aspartate aminotransferase（P〉0.05）. Gd Cl3 pretreatment induced apoptosis and inhibited IL-6 overexpression and STAT3 phosphorylation after PLTx in graft ***： Kupffer cells may contribute to the liver regeneration after PLTx through inhibition of apoptosis and activation of the IL-6/p-STAT3 signal pathway.

关键词： GdCI3 Kupffer cells partial liver transplantation liver regeneration apoptosis

西藏改良奶牛低氧适应的血液生理指标与生产性能分析

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饲料研究 2024年第8期47卷 92-96页

作者：高乐程巴桑珠扎班旦索朗曲吉罗布多吉白玛坚才苟潇旦增洛桑佛山科学技术学院生命科学与工程学院广东佛山528200 西藏自治区农牧科学院畜牧兽医研究所西藏拉萨850000 西藏拉萨市墨竹工卡县农业农村局农业技术推广站西藏拉萨850211 西藏自治区山南市隆子县农牧综合服务中心西藏山南856600

研究旨在测定西藏改良奶牛(荷斯坦奶牛♂×西藏牛♀)在低氧环境下的血液常规指标和凝血生理指标,通过定期检测生产性能测定指标(DHI)与泌乳量对西藏改良奶牛的改良效果进行评估。试验以高原低氧环境下(位于西藏山南市琼结县,海拔3 617 m... 详细信息

研究旨在测定西藏改良奶牛(荷斯坦奶牛♂×西藏牛♀)在低氧环境下的血液常规指标和凝血生理指标,通过定期检测生产性能测定指标(DHI)与泌乳量对西藏改良奶牛的改良效果进行评估。试验以高原低氧环境下(位于西藏山南市琼结县,海拔3 617 m)饲养的改良奶牛作为研究对象,选取从澳大利亚引进西藏的荷斯坦奶牛和西藏牛(西藏本地黄牛)作为对照,测定血液常规指标、凝血指标、DHI指标和头胎次泌乳产量。结果显示,经过长期的低氧环境适应后,西藏改良奶牛的血液生理指标和DHI指标等均发生了明显的变化,主要表现为血红蛋白浓度(HGB)、红细胞比容(HCT)、平均红细胞体积(MCV)显著高于引进荷斯坦奶牛和西藏牛(P0.05),凝血功能较引进荷斯坦奶牛得到改善。与引进荷斯坦奶牛相比,西藏改良奶牛乳脂率、乳蛋白率、乳糖等指标显著升高(P<0.05),体细胞数显著降低(P<0.05)。研究表明,西藏改良奶牛已具备了一定的低氧适应能力,比引进荷斯坦奶牛更能适应低氧环境,相较西藏牛和引进荷斯坦奶牛,西藏改良奶牛的生产性能得到优化和改善。

关键词：西藏改良奶牛血液指标生产性能低氧适应

First case of forearm crisscross injury in children:A case report

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World Journal of Clinical Cases 2021年第25期9卷 7478-7483页

作者： Yi-Kun jiang Yan-Bing Wang Chuan-Gang Peng ji qu Dan-Kai Wu Department of Orthopedics The Second Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina

BACKGROUND Forearm crisscross injury is rare in children;there is no relevant literature so *** lack experience and knowledge in treating this type of crisscross *** report a case of forearm crisscross injury in a child for the first time and analyze its *** SUMMARY An 8-year-old boy experienced pain in his left forearm when he accidentally fell while *** examination revealed swelling and deformity of the left *** performed imaging and the results revealed left radial head dislocation,left distal radial epiphyseal separation from the shaft,and interruption of the continuity of the dorsal cortex of the left distal *** and lateral X-ray films showed that the radius and ulna were crisscrossed.A diagnosis of superior radioulnar joint dislocation,left distal radial epiphyseal injury,and left distal ulnar fracture was *** unsuccessful manual reduction,we adopted a minimally invasive procedure and *** a 14-wk period of follow-up,the patient had good left upper limb function,no complaints of pain or limited range of motion,and good follow-up *** This is the first report of a child with a forearm crisscross injury in which the mechanism and the differences from adult crisscross injury are *** invasive surgery with intramedullary fixation can achieve a good therapeutic *** case provides a reference for the treatment of similar patients in the future.

关键词： Children Forearm Cross injury Fracture Case report

A novel control strategy for reproducing the floor motions of high-rise buildings by earthquake-simulating shake tables

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Earthquake Research Advances 2024年第1期4卷 67-75页

作者： Yuteng Cao Zhe qu Xiaodong ji Key Laboratory of Earthquake Engineering and Engineering Vibration Institute of Engineering MechanicsChina Earthquake AdministrationHarbin150080China Key Laboratory of Earthquake Disaster Mitigation Ministry of Emergency ManagementHarbin150080China Key Laboratory of Civil Engineering Safety and Durability of China Education Ministry Department of Civil EngineeringTsinghua UniversityBeijing100084China

To enable the experimental assessment of the seismic performance of full-scale nonstructural elements with multiple engineering parameters(EDPs),a three-layer testbed named Nonstructural Element Simulator on Shake Table(NEST)has been *** testbed consists of three consecutive floors of steel *** bottom two floors provide a space to accommodate a full-scale *** fully explore the flexibility of NEST,we propose a novel control strategy to generate the required shake table input time histories for the testbed to track the target floor motions of the buildings of interest with high *** control strategy contains two parts:an inverse dynamic compensation via simulation of feedback control systems(IDCS)algorithm and an offline iteration procedure based on a refined nonlinear numerical model of the *** key aspects of the control strategy were introduced in this *** tests were conducted to simulate the seismic responses of a full-scale office room on the 21^(st)floor of a 42-story high-rise *** test results show that the proposed control strategy can reproduce the target floor motions of the building of interest with less than 20%errors within the specified frequency range.

关键词： Shake table test Nonstructural element High-rise building Open-loop IDCS algorithm Off-line iteration

Identification of commercial cultivars of Agaricus bisporus in China using genome-wide microsatellite markers

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Journal of Integrative Agriculture 2019年第3期18卷 580-589页

作者： WANG Li-ning GAO Wei WANG Qiong-ying qu ji-bin ZHANG jin-xia HUANG Chen-yang Key Laboratory of Microbial Resources Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Agaricus bisporus is one of the most widely cultivated mushrooms in the world. Commercial cultivars are usually phenotypically alike and easy to be copied by isolating tissue cultures. This brings great challenges to distinguish different cultivars and to protect new varieties. Thus, techniques for the accurate identification of cultivars are essentially required. In this study, we accurately identified 11 commercial cultivars of A. bisporus released in China by using microsatellite(SSR, simple sequence repeat) markers. SSR markers were developed by mining the genome sequence. A total of 3 134 SSRs were identified, of which 1 490 SSRs were distributed in gene models, and 1 644 in the intergenic regions. A total of 17 polymorphic primer pairs were developed and SSR fingerprints were constructed for all the commercial cultivars. These SSR markers generated a total of 73 alleles, with an average of 4.29 per locus. Specifically, the primer combination of AB_SSR_2341 and AB_SSR_2590 could distinguish all the 11 commercial cultivars. The similarity coefficients of the 11 commercial cultivars were between 0.56 and 0.95 indicating that some of them were close related. Our results provide an efficient technique for the identification of A. bisporus cultivars in China, which can also facilitate the marker-assisted breeding in the future.

关键词： Agaricus bisporus SSR strain identification fingerprint

Overexpression of rice F-box phloem protein gene OsPP12-A13 confers salinity tolerance in Arabidopsis

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BIOCELL 2021年第4期45卷 1121-1135页

作者： CHUNKUN FAN YONGPENG ZHANG CHUNBAO YANG YAWEI TANG ji qu BU jiE DEji quZHEN LIYUN GAO Tibet Academy of Agriculture and Animal Husbandry Sciences Lhasa850009China

Salinity is a serious challenge for agriculture production by limiting the arable *** is a major staple food crop but very sensitive to salt *** this study,we used Arabidopsis for the functional characterization of a rice F-box gene LOC_Os04g48270(OsPP12-A13)under salinity ***12-A13 is a nuclear-localized protein that is strongly upregulated under salinity stress in rice and showed the highest expression in the stem,followed by roots and *** types of transgenic lines for OsPP12-A13 were generated,including constitutive tissue over-expression using the CaMV35S promoter and phloem specific over-expression using the pSUC2 *** types of transgenic plants showed salinity tolerance at the seedling stage through higher germination percentage and longer root length,as compared to control plants under salt stress in MS *** the transgenic plants also exhibited salt tolerance at the reproductive stage through higher survival rate,plant dry biomass,and seed yield per plant as compared to control *** of Na+concentration in leaves,stem and roots of salt-stressed transgenic plants showed that Na^(+) concentration was less in leaf and stem as compared to *** opposite was observed in wild type stressed plants,suggesting that OsPP12-A13 may be involved in Na+transport from root to *** plants also displayed less ROS levels and higher activities of peroxidase and glutathione S-transferase along with upregulation of their corresponding genes as compared to control plants which further indicated a role of OsPP12-A13 in maintaining ROS homeostasis under salt ***,the non-significant difference between the transgenic lines obtained from the two vectors highlighted that OsPP12-A13 principally works in the *** together,this study showed that OsPP12-A13 improves salt tolerance in rice,possibly by affecting Na^(+) transport and ROS homeostasis.

关键词： Antioxidants Rice phloem protein Reactive oxygen species Salt stress Na^(+)transport

Protective roles of trehalose in Pleurotus pulmonarius during heat stress response

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Journal of Integrative Agriculture 2019年第2期18卷 428-437页

作者： LIU Xiu-ming WU Xiang-li GAO Wei qu ji-bin CHEN Qiang HUANG Chen-yang ZHANG jin-xia Key Laboratory of Microbial Resources Ministry of Agriculture and Rural Affaris/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China

High temperature is one of the major abiotic stresses that limit edible mushroom growth and development. The understanding of physiological alterations in response to heat stress and the corresponding mechanisms involved is vital for the breeding of heat-resistant edible mushroom strains. Although trehalose functions as a protectant against abiotic stresses in fungi, the putative role of trehalose in thermotolerance remains to be elucidated. In this study, we found heat stress inhibited the growth of two Pleurotus pulmonarius strains, heat-sensitive and less-sensitive, and the inhibition was more significant for the sensitive strain. Heat stress leads to the increase of lipid peroxidation and intracellular trehalose accumulation, with a higher level in the heat-sensitive strain, and this effect is independent of exogenous trehalose application. In addition, a lower concentration of exogenous trehalose application in sensitive strain than in less-sensitive strain was found to alleviate the inhibition of mycelium growth and further increase the intracellular trehalose concentration by heat stress. Thus, the protective effects of trehalose were more remarkable in the sensitive strain. The activities of intracellular trehalose metabolic enzymes, i.e., trehalose-6-phosphate synthase, trehalose phosphorylase and neutral trehalase, were determined, and our data indicated that the changes of these enzymes activities in the sensitive strain were more beneficial to accumulate trehalose than that in the less-sensitive strain.

关键词： edible mushroom heat stress Pleurotus pulmonarius thermotolerance trehalose

Axial length/corneal radius of curvature ratio and refractive development evaluation in 3-to 4-year-old children: the Shanghai Pudong Eye Study

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International Journal of Ophthalmology(English edition) 2019年第6期12卷 1021-1026页

作者： Ke-Ke Zhao Yuan Yang Hong Wang Lu Li Zhao-Yang Wang Fan jiang ji-Fang qu Shanghai Children’s Medical Center Shanghai Jiao Tong University School of MedicineShanghai 200127China Shanghai Ninth People’s Hospital Shanghai Jiao Tong University School of MedicineShanghai 200011China Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology Shanghai 200011China

AIM: To measure and analysis axial length(AL)/corneal radius of curvature(CRC) ratio and other refractive parameters, provide a medical reference range for refractive development evaluation and earlier visual impairment screening of 3 to 4 y kindergarten ***: Between April and June 2017, a total of 4350 participants aged 3-to 4-year-old(8700 eyes) from 10 cluster random sampling kindergartens in Shanghai, Pudong District were involved. According to the measurement and analysis of the unaided visual acuity(VA), AL, CRC, AL/CRC ratio, astigmatism and other refractive parameters, the data distribution and reference range were ***: Uncorrected VA of examined children was 0.23±0.08(logMAR, mean±SD) [95% confidence interval(CI) range ≤0.36];AL was 22.10±0.79 mm(95%CI 20.55-23.65);CRC was 7.86±0.26 mm(95%CI, 7.35-8.37);AL/CRC ratio was 2.81±0.12(95%CI, 2.57-3.05). The median of astigmatism was-0.5 D, a total of 56.3% had astigmatism -1.50 D;71% were astigmatism with the rule. Eye-specific analyses were conducted. Statistical difference of VA was in right and left eyes. There were no significant differences between two eyes of AL, CRC, AL/CRC ratio and astigmatism(P>0.05).CONCLUSION: VA and AL/CRC ratio reference could be used to assess refractive development in children and screening uncorrected refractive errors or amblyopia. Astigmatism needs to be considered in the diagnosis.

关键词： children axial length/corneal radius of curvature ratio refractive development visual impairment reference