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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-jin Jung Dae-hyun Kim Jun-Koo Yi Jae-Jung Ha ji-hyun lee Seong-Beom lee Woo-Jae Son Soo-Young Yum Won-Wu lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

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Mechanical and electrical properties of In-Bi solder at Bi_(2212) superconductor interface with annealed Ag spray layers and Ag precoating layers

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中国有色金属学会会刊：英文版 2009年第4期19卷 961-964页

作者： Seung-Yong SHIN ji-hyun lee Hai-Woong PARK Department of Materials Engineering Korea University of Technology and Education(KUT)

The electrical properties of solder contact layers between Cu-Ni shunt metal and tube type Bi2212 superconductor that is applied in superconducting fault current limiter were *** contact properties of the solders are improved not only by Ag precoating layers,but also by the pre-sprayed Ag layer and subsequent Ag precoating *** annealed Ag sprayed layers onto Bi2212 superconductor prior to Ag electroplating work as protecting layers for the superconductor from plating *** contact angle of the electroplated Ag layer is 42.91° and decreases to 15.25° and 5.88° with Ag sprayed layer and additional Ag electroplated *** Ag sprayed layer with suitable annealing prior to Ag electroplating improves contact strength of the Ag electroplated layer by about 12% due to denser microstructure of the Ag electroplated layers.

关键词：超导故障限流器 Bi2212 电镀银电气性能喷涂层预涂层退火焊料

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Anti-cancer effect of ethylacetate fraction from Orostachys japonicus on HT-29 human colon cancer cells by induction of apoptosis through caspase-dependent signaling pathway

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Asian Pacific Journal of Tropical Medicine 2018年第5期11卷 330-335页

作者： Deok-Seon Ryu hyun-ji lee ji-Hye Kwon Dong-Seok lee Department of Biomedical Laboratory Science Soonchunhyang University Department of Smart Foods and Drugs Graduate School of Inje University

Objective: To investigate the anti-colon cancer effects of ethylacetate fraction from Orostachys japonicus(0. japonicus) on HT-29 cancer cells. Methods: The viability of HT-29 cells was assayed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H-tetrazolium(MTS) method. Apoptosis induction and cell cycle inhibition were confirmed by fluorescein isothiocyanate and propidium iodide staining using flow *** changes in the nucleus were observed, using a fluorescence microscope with4',6-diamidino-2-phenylindole(DAPI) nuclear staining. The expression levels of the upstream and downstream proteins involved in the anti-cancer mechanism were confirmed by Western blotting. Results: After treating HT-29 cells with different concentrations of ethylacetate fraction from O. japonicus, the viability of cells decreased in a concentration-dependent manner,while apoptosis induction and apoptotic body formation increased. Cell cycle analysis showed that the arrest occurred at the sub-G_1 and S phase. Among the upstream and downstream proteins involved in anti-cancer activity, the level of B cell lymphoma-2 decreased, and the bcl-2-associated x protein increased. The level of pro-caspase-3, pro-caspase-8, and pro-caspase-9 decreased, while the level of cleaved-caspase-3, cleaved-caspase-8, and cleaved-caspase-9 increased. Moreover, the phosphorylation, that is, activation of extracellular signal regulated kinase 1/2, Jun-N-terminal kinase, and p38 increased. Conclusions: Combining the above results, it is thought that the survival of HT-29 cells is suppressed by ethylacetate fraction from0. japonicus through mitochondrial regulation-induced caspase cascade activation, induction of apoptosis and cell cycle arrest.

关键词： Orostachys japonicus HT-29 human colon cancer cells Anti-cancer activity Apoptosis Caspase cascade

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Development of a 3D subcutaneous construct containing insulin-producing beta cells using bioprinting

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Bio-Design and Manufacturing 2022年第2期5卷 265-276页

作者： Chi B.Ahn ji-hyun lee Joo H.Kim Tae H.Kim Hee-Sook Jun Kuk H.Son jin W.lee Department of Molecular Medicine College of MedicineGachon University155Gaetbeol-roYeonsu-kuIncheonRepublic of Korea Department of Health Sciences and Technology GAIHSTGachon University155Gaetbeol-roYeonsu-kuIncheonRepublic of Korea College of Pharmacy Gachon University191Hambangmoe-roYeonsu-kuIncheonRepublic of Korea Department Thoracic and Cardiovascular Surgery Gachon University Gil Medical CenterCollege of MedicineGachon University21Namdong-daero 774 beon-gilNamdong-guIncheonRepublic of Korea

Type 1 diabetes is caused by insulin deficiency due to the loss of beta cells in the islets of *** severe cases,islet transplantation into the portal vein is ***,due to the loss of transplanted islets and the failure of islet function,the 5-year insulin independence rate of these patients is<50%.In this study,we developed a long-term,insulin-secreting,3 Dbioprinted construct implanted subcutaneously with the aim of preventing islet *** bioprinted construct was fabricated by the multi-layer bioprinting of beta-cell(mouse insulinoma-6:MIN-6)-encapsulated alginate bioink and poly(caprolactone)biodegradable polymer.A glucose response assay revealed that the bioprinted constructs proliferated and released insulin normally during the 4-week in vitro *** MIN-6 generated clusters with a diameter of 100-200μm,similar to the original pancreatic islets in the *** an in vivo study using type 1 diabetes mice,animals implanted with bioprinted constructs showed three times higher insulin secretion and controlled glucose levels at 8 weeks after *** the implanted,bioprinted constructs had a positive effect on insulin secretion in the experimental animals,the survival rate of the implanted group(75%)was three times higher than that of the non-implanted group(25%).The results suggest that the proposed,3 D-bioprinted,subcutaneous construct can be a better alternative to portal vein islet transplantation.

关键词： 3D bioprinting Subcutaneous construct Type 1 diabetes Insulin Beta cells

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Clinicopathologic significance of expression of nuclear factor-kB RelA and its target gene products in gastric cancer patients

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World Journal of Gastroenterology 2012年第34期18卷 4744-4750页

作者： Hyuk-Chan Kwon Sung-hyun Kim Sung Yong Oh Suee lee ji hyun lee jin Seok Jang Min Chan Kim Ki Han Kim Su-jin Kim Seong-Geun Kim Hyo-jin Kim Department of Internal Medicine Dong-A University College of MedicineBusan 602-715South Korea Department of Surgery Dong-A University College of MedicineBusan 602-715South Korea Department of Pathology Dong-A University College of MedicineBusan 602-715South Korea Research Institute for Convergence of Biomedical Science and Technology Pusan National University Yangsan HospitalBusan 602-715South Korea

AIM:To assess the prognostic significance of nuclear factor-kB (NF-kB) and its target genes in gastric cancer. METHODS:The tumor tissues of 115 patients with gastric cancer were immunohistochemically evaluated using monoclonal antibodies against NF-kB RelA. Preoperative serum levels of vascular endothelial growth factor (VEGF), interleukin-6 (IL-6) were assessed via enzyme-linked immuno-sorbent assay. C-reactive protein (CRP) and serum amyloid A (SAA) were measured via immunotrubidimetry. RESULTS:Positive rate of NF-kB RelA was 42.6%. NF-kB RelA expression in tumor tissues was also related to serum levels of IL-6 (P = 0.044) and CRP (P = 0.010). IL-6, SAA, CRP were related to depth of invasion, VEGF and SAA were correlated with lymph node metastasis. IL-6, VEGF, SAA and CRP were related to the stage. Univariate analysis demonstrated that immunostaining of NF-kB RelA, levels of IL-6, VEGF, SAA were significantly related with both disease free survival and over-all survival (OS). Multivariate analysis verified that NF-kB RelA [hazard ratio (HR): 3.40, P = 0.024] and SAA (HR: 3.39, P = 0.045) were independently associated with OS. CONCLUSION: Increased expression of NF-kB RelA and high levels of serum SAA were associated with poor OS in gastric cancer patients.

关键词： Nuclear factor-κB Vascular endothelial grow-th factor Interleukin-6 C-reactive protein Serum amy-loid A Stomach Carcinoma

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Behavioral inhibition in female college students with schizotypal traits: An event-related potential study

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Open Journal of Psychiatry 2012年第4期2卷 362-369页

作者： ji-hyun lee Myung-Sun Kim Department of Psychology Sungshin Women’s University Seoul Korea

This study investigated behavioral inhibition in female college students with psychometrically defined schizotypal traits using a Go/NoGo task and event-related potentials (ERPs). The schizotypal-trait (n = 15) and normal control (n = 15) groups were selected based on scores of the Schizotypal Personality Questionnaire (SPQ). The Go/NoGo task consisted of Go (requires response) and NoGo (requires no response) conditions. In terms of response time and accuracy rate for the Go/NoGo task, the two groups did not differ significantly. In terms of ERPs, the control group showed greater N2 amplitudes in response to NoGo (NoGo-N2) than to Go stimuli (Go-N2), whereas the schizotypal-trait group showed no significant difference in NoGo-N2 and Go-N2 amplitudes. In addition, the schizotypal-trait group showed reduced NoGo-N2 amplitudes at the frontal site compared to controls, and an association between SPQ scores and NoGo-N2 amplitudes measured at the frontal site. The two groups did not differ in P3 amplitudes. Since the N2 reflects the detection of response conflict and behavioral inhibition, the present results indicate that nonclinical individuals with schizotypal traits have difficulties in detecting response conflict and behavioral inhibition.

关键词： Behavioral Inhibition Event-Related Potentials Go/NoGo Task N2 P3 Schizotypal Trait

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Small but big leaps towards neuroglycomics:exploring N-glycome in the brain to advance the understanding of brain development and function

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Neural Regeneration Research 2024年第3期19卷 489-490页

作者： Boyoung lee hyun Joo An Center for Cognition and Sociality Institute for Basic Science(IBS)DaejeonSouth Korea Asia-Pacific Glycomics Reference Site DaejeonSouth Korea Graduate School of Analytical Science&Technology Chungnam National UniversityDaejeonSouth Korea

Glycosylation is a process that involves the addition of sugar moieties or glycans to different types of molecules,including proteins,lipids,and nucleic *** these,protein glycosylation is one of the most prevalent forms of post-translational modification,playing a crucial role in biological *** more than ten monosaccharides identified within mammalian brain cells and more than 1×1012 possible combinations,the heterogeneity of glycosylation is extensive(Conroy et al.,2021).The diversity of glycans and the complexity of their structures allow for a wide range of protein functions.N-glycans are one of the most abundant forms of glycans and are involved in various cellular functions.N-glycans can be added to proteins at specific sequons,Asn-X-Ser/Thr,and are classified into three main types in mature glycoproteins:high mannose,complex,and *** mannose N-glycans consist of 5-9 mannose residues linked to a chitobiose core and undergo processing into complex or hybrid forms in the Golgi apparatus(Varki et al.,2017).Complex N-glycans are more diverse and contain various branched structures such as antennae with fucose,galactose,and sialic acid *** N-glycans contain one or more complex branches in conjunction with an oligomannose branch(Fisher and Ungar,2016).Understanding the specific functions of these different types of N-glycans in protein regulation,folding,and function is an active area of research in the life sciences,including glycobiology.

关键词： functions function apparatus

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Novel Rifle Number Recognition Based on Improved YOLO in Military Environment

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Computers, Materials & Continua 2024年第1期78卷 249-263页

作者： hyun Kwon Sanghyun lee Department of Artificial Intelligence and Data Science Korea Military AcademySeoulKorea Graduate School of Information Security Korea Advanced Institute of Science and TechnologyDaejeonKorea

Deep neural networks perform well in image recognition,object recognition,pattern analysis,and speech *** military applications,deep neural networks can detect equipment and recognize *** military equipment,it is necessary to detect and recognize rifle management,which is an important piece of equipment,using deep neural *** have been no previous studies on the detection of real rifle numbers using real rifle image *** this study,we propose a method for detecting and recognizing rifle numbers when rifle image data are *** proposed method was designed to improve the recognition rate of a specific dataset using data fusion and transfer *** the proposed method,real rifle images and existing digit images are fusedas trainingdata,andthe final layer is transferredto theYolov5 *** detectionand recognition performance of rifle numbers was improved and analyzed using rifle image and numerical *** used actual rifle image data(K-2 rifle)and numeric image datasets,as an experimental *** was used as the machine learning *** results show that the proposed method maintains 84.42% accuracy,73.54% precision,81.81% recall,and 77.46% F1-score in detecting and recognizing rifle *** proposed method is effective in detecting rifle numbers.

关键词： Machine learning deep neural network rifle number recognition detection

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Dual-logic-in-memory implementation with orthogonal polarization of van der Waals ferroelectric heterostructure

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InfoMat 2024年第2期6卷 107-117页

作者： jingjie Niu Sumin Jeon Donggyu Kim Sungpyo Baek hyun Ho Yoo jie Li ji-Sang Park Yoonmyung lee Sungjoo lee SKKU Advanced Institute of Nanotechnology(SAINT) Sungkyunkwan UniversitySuwonRepublic of Korea Department of Nano Science and Technology Sungkyunkwan UniversitySuwonRepublic of Korea Department of Electrical and Computer Engineering Sungkyunkwan UniversitySuwonRepublic of Korea IMEC HeverleeBelgium Department of Nano Engineering Sungkyunkwan UniversitySuwonRepublic of Korea

The rapid advancement of AI-enabled applications has resulted in an increasing need for energy-efficient computing ***-in-memory is a promising approach for processing the data stored in memory,wherein fast and efficient computations are possible owing to the parallel execution of reconfigurable logic *** this study,a dual-logic-in-memory device,which can simultaneously perform two logic operations in four states,is demonstrated using van der Waals ferroelectric field-effect transistors(vdW FeFETs).The proposed dual-logic-in-memory device,which also acts as a twobit storage device,is a single bidirectional polarization-integrated ferroelectric field-effect transistor(BPI-FeFET).It is fabricated by integrating an in-plane vdW ferroelectric semiconductor SnS and an out-of-plane vdW ferroelectric gate dielectric material—CuInP_(2)S_(6).Four reliable resistance states with excellent endurance and retention characteristics were *** two-bit storage mechanism in a BPI-FeFET was analyzed from two perspectives:carrier density and carrier injection controls,which originated from the out-of-plane polarization of the gate dielectric and in-plane polarization of the semiconductor,*** conventional multilevel FeFETs,the proposed BPIFeFET does not require additional pre-examination or erasing steps to switch from/to an intermediate polarization,enabling direct switching between the four memory *** utilize the fabricated BPI-FeFET as a dual-logic-inmemory device,two logical operations were selected(XOR and AND),and their parallel execution was *** types of logic operations could be implemented by selecting different initial states,demonstrating various types of functions required for numerous neural network *** flexibility and efficiency of the proposed dual-logic-in-memory device appear promising in the realization of next-generation low-power computing systems.

关键词： ferroelectric field-effect transistor in-plane ferroelectricity logic-in-memory out-of-plane ferroelectricity

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Real-world outcomes of ibrutinib therapy in Korean patients with relapsed or refractory mantle cell lymphoma:amulticenter,retrospective analysis

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Cancer Communications 2021年第3期41卷 275-278页

作者： Jun Ho Yi Seok jin Kim Dok hyun Yoon Cheolwon Suh Myung Hee Chang Deok Hwan Yang Jae-Cheol Jo Shin Young hyun Hyeon-Seok Eom Jeong-Ok lee ji hyun Kwon Sang Hoon Han Seung-Shin lee Jae-Yong Kwak Se hyung Kim Dae Sik Kim ji hyun lee Sung Yong Oh Hun Mo Ryoo Hyo Jung Kim Won Seog Kim Division of Hematology-Oncology Department of MedicineChung-Ang UniversityDongjak-guSeoul 06973Korea Division of Hematology and Oncology Department of MedicineSamsung Medical Center Sungkyunkwan University School of MedicineGangnam-guSeoul 06351Korea Department of Oncology Asan Medical CenterUniversity of Ulsan College of MedicineSongpa-guSeoul 05505Korea National Health Insurance Corporation Ilsan Hospital Ilsandong-guGoyang 10444Korea Department of Hemato-Oncology Chonnam National University Hwasun HospitalChonnam National University Medical SchoolHwasunGwangju 58128Korea Department of Hematology and Oncology Ulsan University HospitalUniversity of Ulsan College of MedicineDong-guUlsan 44033Korea Department of Internal Medicine Yonsei University Wonju College of MedicineGangwon-DoWonju 26426Korea Department of Internal Medicine National Cancer CenterGoyang-si Gyeonggi-doGoyang 10408Korea Department of Internal Medicine Seoul National University Bundang HospitalBundang-guSeongnam 13620Korea Department of Internal Medicine ChungBuk National University HospitalChungbukCheongju 28644Korea Department of Internal Medicine Jeju National University HospitalJeju National University School of MedicineJeju 63241Korea Department of Hematology-Oncology Wonkwang University HospitalIksan-daeroIksan 54538Korea Department of Internal Medicine Jeonbuk National University Medical SchoolJeollabuk-doJeonju 54907Korea Division of Hematology-Oncology Department of Internal MedicineSoonchunhyang University College of MedicineBucheon HospitalWonmi-guBucheon 14584Korea Division of Hematology-Oncology Department of Internal MedicineKorea University Guro HospitalGuro-guSeoul 08308Korea Division of Hematology-Oncology Department of Internal MedicineDong-A University College of MedicineSeo-guBusan 49201Korea Department of Internal Medicine Division of Hematology-Oncology Catholic University of DaeguDaegu 42472Korea Division of Hematology-Oncology Department of Internal MedicineHallym U

Dear Editor,Despite the introduction of novel front-line therapies including rituximab plus high-dose cytarabine followed by consolidative autologous stem cell transplantation(ASCT)and salvage therapy with bendamustine,lenalidomide or bortezomib,mantle cell lymphoma(MCL)is still considered incurable and most patients experience relapse or refractory(RR)disease.

关键词： patients relapse lymphoma

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