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Genomics,transcriptomics,and peptidomics of the greater wax moth Galleria mellonella neuropeptides and their expression in response to lead stress

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Insect Science 2024年第3期31卷 773-791页

作者： Li-Lin Luo Yang Lin Jun-hong Linghu Wei Gong Yuan-hong Luo man Liu Dao-Chao jin Guy Smagghe Tong-Xian Liu Shun-Hua Gui Tian-Ci Yi Guizhou Provincial Key Laboratory for Agricultural Pest management of the Mountainous Region Institute of EntomologyGuizhou UniversityGuiyangGuizhouChina Guizhou Institute of Biology Guizhou Academy of SciencesGuiyangChina Institute of Plant Health and Medicine Guizhou UniversityGuiyangChina

Neuropeptides are crucial in regulation of a rich variety of developmental,physiological,and behavioral functions throughout the life cycle of *** an integrated approach of multiomics,we identified neuropeptide precursors in the greater wax moth Galleria mellonella,which is a harmful pest of honeybee hives with a worldwide ***,a total of 63 and 67 neuropeptide precursors were predicted and annotated in the *** genome and transcriptome,in which 40 neuropeptide precursors were confirmed in the *** ***,we identified 12 neuropeptide precursor genes present in *** but absent in honeybees,which may be potential novel pesticide target *** hives were contaminated with heavy metals such as lead,enabling its bioaccumulation in *** bodies through the food chain,we performed transcriptome sequencing to analyze the effects of Pb stress on the mRNA expression level of *** neuropeptide *** treatment by Pb,the expression of neuropeptide F1 was found to be significantly downregulated,implying that this neuropeptide might be associated with responding to the heavy metal stress in *** study comprehensively identified neuropeptide precursors in ***,and discussed the effects of heavy metals on insect neuropeptides,with the example of *** results are valuable for future elucidation of how neuropeptides regulate physiological functions in *** and contribute to our understanding of the insect's environmental plasticity and identify potential new biomarkers to assess heavy metal toxicity in insects.

关键词： Galleria mellonella genomics heavy metal stress neuropeptide peptidomics transcriptomics

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sTREM-1 as promising prognostic biomarker for acute-on-chronic liver failure and mortality in patients with acute decompensation of cirrhosis

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World Journal of Gastroenterology 2024年第9期30卷 1177-1188页

作者： Song-man Yu Hai Li Guo-hong Deng Xian-Bo Wang Xin Zheng jin-Jun Chen Zhong-ji Meng Yu-Bao Zheng Yan-Hang Gao Zhi-Ping Qian Feng Liu Xiao-Bo Lu Yu Shi jia Shang Ruo-Chan Chen Yan Huang Department of Infectious Diseases Xiangya HospitalCentral South UniversityChangsha 410000Hunan ProvinceChina Department of Gastroenterology Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Department of Infectious Diseases Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Center of Integrative Medicine Beijing Ditan HospitalCapital Medical UniversityBeijing 100020China Department of Infectious Diseases Institute of Infection and ImmunologyUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430020Hubei ProvinceChina Department of Infectious Diseases Nanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Department of Infectious Disease Taihe HospitalHubei University of MedicineShiyan 442009Hubei ProvinceChina Department of Infectious Diseases The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630Guangdong ProvinceChina Department of Hepatology The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Department of Liver Intensive Care Unit Shanghai Public Health Clinical CentreFudan UniversityShanghai 200093China Tianjin Institute of Hepatology Nankai University Second People's HospitalTianjin 300192China Infectious Disease Center The First Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830054Xinjiang Uygur Autonomous RegionChina The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital of School of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvineChina Department of Infectious Diseases Henan Provincial People's HospitalZhengzhou 463599Henan ProvineChina Department of Infectious Diseases Hunan Key Laboratory of Viral HepatitisXiangya HospitalCentral South UniversityChangsha 110051Hunan ProvineChina

BACKGROUND Acute decompensation(AD)of cirrhosis is associated with high short-term mortality,mainly due to the development of acute-on-chronic liver failure(ACLF).Thus,there is a need for biomarkers for early and accurate identification of AD patients with high risk of development of ACLF and *** triggering receptor expressed on myeloid cells-1(sTREM-1)is released from activated innate immune cells and correlated with various inflammatory *** To explore the prognostic value of sTREM-1 in patients with AD of *** A multicenter prospective cohort of 442 patients with cirrhosis hospitalized for AD was divided into a study cohort(n=309)and validation cohort(n=133).Demographic and clinical data were collected,and serum sTREM-1 was measured at *** enrolled patients were followed-up for at least 1 *** In patients with AD and cirrhosis,serum sTREM-1 was an independent prognosis predictor for 1-year survival and correlated with liver,coagulation,cerebral and kidney failure.A new prognostic model of AD(P-AD)incorporating sTREM-1,blood urea nitrogen(BUN),total bilirubin(TBil),international normalized ratio(INR)and hepatic encephalopathy grades was established and performed better than the model for end-stage liver disease(MELD),MELD-sodium(MELD-Na),chronic liver failure-consortium(CLIF-C)ACLF and CLIF-C AD ***,sTREM-1 was increased in ACLF and predicted the development of ACLF during first 28-d *** ACLF risk score incorporating serum sTREM-1,BUN,INR,TBil and aspartate aminotransferase levels was established and significantly superior to MELD,MELD-Na,CLIF-C ACLF,CLIF-C AD and P-AD in predicting risk of ACLF *** Serum sTREM-1 is a promising prognostic biomarker for ACLF development and mortality in patients with AD of cirrhosis.

关键词： Soluble triggering receptor expressed on myeloid cell-1 Acute decompensation Cirrhosis Acute-on-chronic liver failure Prognostic biomarker

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Recent advances in chemical protein synthesis:method developments and biological applications

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Science China Chemistry 2024年第4期67卷 1060-1096页

作者： Suwei Dong ji-Shen Zheng Yiming Li Huan Wang Gong Chen Yongxiang Chen Gemin Fang Jun Guo Chunmao He honggang Hu Xuechen Li Yanmei Li Zigang Li man Pan Shan Tang Changlin Tian Ping Wang Bian Wu Chuanliu Wu Junfeng Zhao Lei Liu State Key Laboratory of Natural and Biomimetic Drugs and School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei 230027China School of Food and Biological Engineering Engineering Research Center of Bio-processMinistry of EducationHefei University of TechnologyHefei 230009China School of Chemistry and Chemical Engineering Nanjing UniversityNanjing 210023China State Key Laboratory of Elemento-Organic Chemistry Nankai UniversityTianjin 300071China Department of Chemistry Tsinghua UniversityBeijing 100084China Institute of Health Sciences Anhui UniversityHefei 230601China College of Chemistry Central China Normal UniversityWuhan 430079China School of Chemistry and Chemical Engineering South China University of TechnologyGuangzhou 510640China School of Medicine or Institute of Translational Medicine Shanghai UniversityShanghai 200444China Department of Chemistry The University of Hong KongHong Kong 999077China Shenzhen Graduate School Peking UniversityShenzhen 518055China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Life Sciences University of Science and Technology of ChinaHefei 230026China Department of Chemical Biology Shanghai Jiao Tong UniversityShanghai 200240China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China Department of Chemistry Xiamen UniversityXiamen 361005China School of Pharmaceutical Science Guangzhou Medical UniversityGuangzhou 511436China

The central dogma of modern biology underscores the pivotal roles proteins play in diverse biological processes,the study of which necessitates advanced methods to produce proteins with precision and *** protein synthesis,a powerful approach utilizing chemical reactions for the de novo construction of structurally accurate proteins,has emerged as a transformative tool for studying proteins and generating protein derivatives/mimics inaccessible by natural biological machinery,including post-translationally modified proteins,proteins comprised of unnatural amino acids,as well as mirror-image *** review summarizes recent strides in synthetic method developments for chemical protein synthesis,including innovative techniques in solid-phase peptide synthesis,the challenges presented by difficult sequences in either synthesis or folding and the exploration of novel ligation reactions using both chemical and enzymatic ***,the review also delves into newly developed protocols for site-selective protein modifications and the generation of stapled or macrocyclized peptides/miniproteins,highlighting the power of chemical methods to make structurally diverse *** applications of synthetic proteins in investigating post-translational modifications(phosphorylation,lipidation,glycosylation,ubiquitination,etc.),mirror-image biological processes and drug development are further ***,these topics provide a comprehensive overview of the current landscape of chemical protein synthesis.

关键词： chemical protein synthesis solid-phase peptide synthesis ligation reactions post-translational modifications mirror-image proteins peptide drugs

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Expert Consensus on the Treat-to-Target Strategy for Psoriasis With Biological Agents in China(Simplified Edition)

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International Journal of Dermatology and Venereology 2024年第2期7卷 61-69页

作者： Ai-Jun Chen Da-Xing Cai Xiang Chen Yong Cui Yan Ding Heng Gu Xing-Hua Gao Chao ji Xiao-jing Kang Ye-hong Kuang Cheng-Zhi Lyu Min Li Wei Li Xia Li Yu-Zhen Li Xiao-Yong man jian-Jun Qiao Juan Su Liang-Dan Sun Yu-Ling Shi Juan Tao jian-Xin Xia Rong Xiao Bin Yang Wu Zhu Xue-Jun Zhang Committee on Psoriasis Dermatology and Venereology Branch of the Chinese Medical Association Department of Dermatology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Dermatology Qilu HospitalShandong UniversityJinanShandong 250100China Department of Dermatology Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Dermatology China-Japan Friendship HospitalBeijing 100192China Department of Dermatology Hainan Provincial Hospital of Skin DiseaseHaikouHainan 570311China Department of Dermatology Hospital for Skin DiseasesChinese Academy of Medical SciencesNanjingJiangsu 210042China Department of Dermatology The First Hospital of China Medical UniversityShenyangLiaoning 110002China Department of Dermatology The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian 350004China Department of Dermatology People’s Hospital of Xinjiang Uygur Autonomous RegionUrumqiXinjiang Uyghur Autonomous Region 830001China Department of Dermatology Dalian Dermatosis HospitalDalianLiaoning 116021China Department of Dermatology Dushu Lake Hospital Affiliated to Soochow UniversitySuzhouJiangsu 215127China Department of Dermatology West China Hospital of Sichuan UniversityChengduSichuan 610044China Department of Dermatology Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Dermatology The Second Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiang 150086China Department of Dermatology The Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiang 310003China Department of Dermatology The First Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiang 310003China Department of Dermatology The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230022China Department of Dermatology Shanghai Skin Disease HospitalTongji University School of MedicineShanghai 200093China Department of Dermatology The Union Hospital of Tongji Medical CollegeHuazhong University of Science and Te

Psoriasis is a chronic,recurrent,inflammatory systemic disease that is not only characterized by skin manifestations but may also be accompanied by various comorbidities,imposing a heavy burden on patients’physical and mental health and affecting their quality of *** the continuous approval of various biological agents for clinical use has provided more effective treatment options to patients with psoriasis,no consensus on the evaluation of comprehensive treatment goals has yet been *** treat-to-target(T2T)strategy requires consideration of multiple dimensions of treatment outcomes,the development of long-term management goals,and regular assessments of treatment conditions,which are often used in the management of chronic ***,based on the latest consensuses and guidelines,research data,and clinical experience as well as the combination of survey results and expert group discussions,the present consensus focuses on 4 dimensions of short-and long-term integrated management goals for biological agents:alleviating skin lesions,improving quality of life,screening and managing psoriasis comorbidities,and ensuring drug *** implementation methods,evaluation time,treatment monitoring,and program adjustments are also herein described to achieve comprehensive management of psoriasis to the maximum *** consensus provides a reference for clinical practice.

关键词： psoriasis biological agents treat-to-target comorbidity consensus

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Non-isothermal reduction process analysis of iron-bearing burden with charging iron coke hot briquette under simulated blast furnace conditions

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Journal of Iron and Steel Research(International) 2022年第5期29卷 741-750页

作者： hong-tao Wang man-sheng Chu ji-wei Bao Zheng-gen Liu hong-ming Long School of Metallurgy Engineering Anhui University of TechnologyMa’anshan243032AnhuiChina State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang110819LiaoningChina School of Metallurgy Northeastern UniversityShenyang110819LiaoningChina Key Laboratory of Metallurgical Emission Reduction&Resources Recycling Anhui University of TechnologyMinistry of EducationMa’anshan243032AnhuiChina

Highly reactive iron coke hot briquette(ICHB)prepared by carbonizing the agglomerate of iron-bearing substance and blended coals is regarded as an alternative fuel to mitigate carbon emission and energy consumption of blast ***,the reduction process of iron-bearing burden is extremely crucial for blast furnace *** effects of ICHB on the non-isothermal reduction process of iron-bearing burden with different reduction properties were thus experimentally studied under the conditions of simulated blast furnace lump zone(below 1100°C),and the related mechanism was discussed and *** results showed that the non-isothermal reduction process of iron-bearing burden is promoted by adding *** the charging ratio of ICHB is increased from 0%to 30%,the reduction degree of pellet is increased from 22.91%to 36.62%,but the increased amplitude is leveled ***,the reduction degree of sinter is raised from 35.10%to 93.33%.It is also indicated that the promotion effect of ICHB on the non-isothermal reduction of iron-bearing burden depends on the reduction property of *** with the burden with poor reduction performance(such as pellet 1),the promotion is more significant for the burden with good reduction property(such as sinter 1)since the reduction reaction of iron oxide in iron-bearing burden and the gasification of carbon in ICHB are remarkably reinforced each *** practical application of ICHB in blast furnace should be utilized with highly reductive iron-bearing burden.

关键词： Iron coke hot briquette Non-isothermal reduction Iron-bearing burden Low carbon emission Blast furnace

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超宽公路架桥机优化设计与应用:以贵州省新平河特大桥为例

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科学技术与工程 2024年第17期24卷 7342-7350页

作者：韩洪举李建勋郭吉平胡嫚赵疆陶铁军吴飞贵州路桥集团有限公司贵阳550001 西南大学工程技术学院重庆400715 贵州大学土木工程学院贵阳550025 重庆大学机械与运载工程学院重庆400044

针对在超宽梁架设过程中,由于桥墩的墩顶直径较小,使得前支腿间距过窄,横梁会与架桥机前支腿产生干涉,并且现有架桥机无法满足喂梁和架设要求这一问题,提出了超宽公路架桥机前支腿内收开合的设计方案。通过理论分析,三维建模以及有限元... 详细信息

针对在超宽梁架设过程中,由于桥墩的墩顶直径较小,使得前支腿间距过窄,横梁会与架桥机前支腿产生干涉,并且现有架桥机无法满足喂梁和架设要求这一问题,提出了超宽公路架桥机前支腿内收开合的设计方案。通过理论分析,三维建模以及有限元分析的方法,提出了架桥机双前支腿独立运动方案,将架桥机前支腿由传统的纯箱式结构改造为具有主支腿,液压升降支腿,副支腿的新型前支腿结构,通过前支腿的内收可以使架桥机前支腿架设在普通直径的桥墩上;通过前支腿开合结构设计,可以使超宽梁通过前支腿,不造成干涉。成功解决贵州省钢-混桥梁实际施工建设中的技术难点,实现了超宽连续超宽钢主梁的整孔高效架设,并以贵州省都匀—安顺高速公路新平河特大桥铺设为例,进行了有限元分析与现场应力、挠度监测,验证了所提出架桥机的结构安全性能,为超宽公路架桥的应用提供参考。

关键词：桥梁工程超宽新型公路架桥机三维建模公路架桥机超宽梁架设

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“植物生理学”理论结合实践的教学模式改革

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教育教学论坛 2024年第4期 51-54页

作者：肖继坪于虹漫陈疏影云南农业大学农学与生物技术学院云南昆明650201

“植物生理学”是高等农林院校植物生产类本科生的专业基础课,教学涉及的专业多,授课对象的知识结构和学习特点不断变化,且面临学时少、新知识和新技术层出不穷的窘境。因此,从甄选教学内容、丰富教学题材,改进教学方法,学以致用。建立... 详细信息

“植物生理学”是高等农林院校植物生产类本科生的专业基础课,教学涉及的专业多,授课对象的知识结构和学习特点不断变化,且面临学时少、新知识和新技术层出不穷的窘境。因此,从甄选教学内容、丰富教学题材,改进教学方法,学以致用。建立“植物生理学”开放课堂,科研反哺教学、指导学生参加创新创业大赛、增强科研创新意识等方面对“植物生理学”进行了教学改革与实践。同时,“植物生理学”教学的研究对象选用本地的植物类型或地方品种并列举它们与当地生态环境之间的关系,使教学内容既贴近生活实际又符合学校的办学特色,既能提高学生的综合素质与实践能力,又可为培养创新型人才奠定基础。

关键词：植物生理学教学内容教学方法理论联系实际科研创新

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Adaptive sliding mode control of modular self-reconfigurable spacecraft with time-delay estimation

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Defence Technology（防务技术） 2022年第12期18卷 2170-2180页

作者： Xin-hong Li Zhi-bin Zhang ji-ping An Xin Zhou Gang-xuan Hu Guo-hui Zhang Wan-xin man Department of Aerospace Science and Technology Space Engineering UniversityNo.1 BaYi RoadHuaiRou DistrictBeijing101416China Beijing Institute of Remote Sensing Information Beijing100192China

The reconstruction control of modular self-reconfigurable spacecraft (MSRS) is addressed using an adaptive sliding mode control (ASMC) scheme based on time-delay estimation (TDE) technology. In contrast to the ground, the base of the MSRS is floating when assembled in orbit, resulting in a strong dynamic coupling effect. A TED-based ASMC technique with exponential reaching law is designed to achieve high-precision coordinated control between the spacecraft base and the robotic arm. TDE technology is used by the controller to compensate for coupling terms and uncertainties, while ASMC can augment and improve TDE’s robustness. To suppress TDE errors and eliminate chattering, a new adaptive law is created to modify gain parameters online, ensuring quick dynamic response and high tracking accuracy. The Lyapunov approach shows that the tracking errors are uniformly ultimately bounded (UUB). Finally, the on-orbit assembly process of MSRS is simulated to validate the efficacy of the proposed control scheme. The simulation results show that the proposed control method can accurately complete the target module’s on-orbit assembly, with minimal perturbations to the spacecraft’s attitude. Meanwhile, it has a high level of robustness and can effectively eliminate chattering.

关键词： Adaptive sliding mode control(ASMC) Time delay control Time delay estimation Modular self-reconfigurable spacecraft Uncertainty Coordinated control

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米缟螟蛹及成虫雌雄个体的快速鉴别方法

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环境昆虫学报 2024年第2期46卷 524-529页

作者：饶永超罗丽林王济红黄振兴刘曼姚松林杨茂发贵州省生物研究所贵阳550009 贵州大学贵阳550025

为建立一种快速鉴别米缟螟Aglossa dimidiata蛹及成虫雌雄的方法,分别利用超景深数码显微系统及扫描电镜对米缟螟蛹和成虫的外部特征进行观察并统计。结果表明:雌蛹第8腹节腹面上有一扁平的纵裂缝为生殖孔,生殖孔离肛门的距离较远,分节... 详细信息

为建立一种快速鉴别米缟螟Aglossa dimidiata蛹及成虫雌雄的方法,分别利用超景深数码显微系统及扫描电镜对米缟螟蛹和成虫的外部特征进行观察并统计。结果表明:雌蛹第8腹节腹面上有一扁平的纵裂缝为生殖孔,生殖孔离肛门的距离较远,分节不明显;而雄蛹的生殖孔在第9腹节,生殖孔两侧各有一个半瘤状突起,且生殖孔离肛门的距离较近,分节较明显。雌性成虫腹部较为粗壮,腹部末端呈圆筒状且有圆形环毛,雄性成虫腹部至末端较为瘦小,呈倒三角状且有味刷。利用该方法共鉴定雌雄蛹200头,成虫200头,准确率达100%。这些结果将为米缟螟的性行为学、化学生态学以及资源昆虫开发利用等方面的研究奠定基础。

关键词：米缟螟蛹成虫雌雄鉴别

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基于植被指数的生态恢复力评价及影响因素研究——以武陵山片区为例

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江西农业学报 2024年第3期36卷 93-101页

作者：杨蔓红尹宁玲左金友杨益民吴吉林吉首大学土木工程与建筑学院湖南张家界427000

选取归一化植被指数(NDVI)作为生态恢复力替代指标,从敏感性和适应性2个维度构建了生态恢复力测度模型,对2000—2020年武陵山片区生态恢复力进行了综合评价,并通过多元线性回归模型探究了武陵山片区生态恢复力的主要影响因素。结果表明:... 详细信息

选取归一化植被指数(NDVI)作为生态恢复力替代指标,从敏感性和适应性2个维度构建了生态恢复力测度模型,对2000—2020年武陵山片区生态恢复力进行了综合评价,并通过多元线性回归模型探究了武陵山片区生态恢复力的主要影响因素。结果表明:(1)2000—2020年武陵山片区生态恢复力指数平均为0.45,整体呈北高南低、西高东低的空间分布格局;(2)低、较低、中等、较高和高生态恢复力地区分别占武陵山总片区面积的0.29%、18.19%、35.82%、29.01%和16.69%;(3)生态恢复力与年平均气温、坡度、建设用地面积占比、碳排放量呈负相关,与年降水量、高程、人口密度、GDP呈正相关;其中年平均气温、人口密度和碳排放量是武陵山片区生态恢复力的主要影响因素。

关键词：生态恢复力归一化植被指数影响因素武陵山片区

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