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Comparison of lipid extraction methods for the microalgae Acutodesmus obliquus

International Journal of Agricultural and Biological Engineering 2018年第5期11卷 211-217页

作者： Diana Ximena Hurtado Claudia Lorena Garzón-Castro jhon cortés-romero Edisson Tello Camacho Bioprospecting and CAPsAB Research Groups Faculty of EngineeringUniversidad de La SabanaCundinamarca 53753Colombia Biosciences Doctoral Program Faculty of EngineeringUniversidad de La SabanaCundinamarca 53753Colombia Department of Electrical and Electronic Engineering Universidad Nacional de ColombiaBogotá53753Colombia

Microalgae are widely used in the pharmaceutical and energy industries,therefore the conditions for their cultivation and extraction methods play an important role in the profiling and acquisition of *** efficiency of lipid extraction from microalgae has attracted great interest from industry because of the wide variety of lipids and amounts that can be *** obliquus(scenedesmus obliquus UTEX 393)was used in this *** was cultivated in Bold 3N medium modified with 75%nitrogen at 25℃,pH 6.8,125 r/min and a photoperiod of 18/6 h and illuminated with white light provided by a Light-Emitting Diode surface Mount Device extensions(LED sMD)with an intensity of 1200μE/(m^(2)·s).The cells were stained with the Red Nile(RN)technique to indicate lipid *** extraction methods were compared,classical,microwave(MW),soxhlet,and ultrasound(Us),using the same solvent proportions(hexane:chloroform:methanol=1:2:3).All samples were analyzed with Fourier Transform Infrared spectroscopy(FTIR)and Gas Chromatography coupled to Mass spectrometry(GC-Ms).The results showed:1)lipid production detected by RN was consistent with microalgal growth;2)the MW technique was the best extraction method,according to the statistical analysis through Randomized Complete Block(RCB)design and performance of 4.6%;and 3)the presence of saturated and unsaturated acids was indicated by FTIR ***-Ms was able to identify palmitic and linoleic acids as the likely major constituents of the sample.

关键词： Acutodesmus obliquus lipid extraction trans-esterification Red Nile(RN)

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Effect of monovalent/divalent ions and siO2-based nanocomposite dosage on thermochemical stability of HPAM polymeric solutions

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Petroleum 2024年第4期 719-735页

作者： jhon F.Gallego Lady J.Giraldo Henderson I.Quintero Hugo A.García Karol Zapata samira Heidari Masoud Riazi Camilo A.Franco Farid B.cortés Grupo de Investigación en Fenómenos de superficie-Michael Polanyi Facultad de Minas Universidad Nacional de Colombia-Sede Medellín Ecopetrol s.A-Instituto Colombiano del Petróleo school of Mining and Geosciences Nazarbayev University

This study evaluated the effect of monovalent and divalent ions and the dosage of a siO2-based nanocomposite on the thermochemical stability of HPAM polymeric solution. Chelating amine-functionalized NPs（AFNPs） were used to enhance the thermochemical stability of HPAM based on capturing monovalent/divalent ions after seven days at 70°C. Different polymer solutions prepared with calcium chloride dihydrate（CaCl2·2H2O） at 2000 mg/L and sodium chloride（NaCl） at 10000 mg/L, and two different dosages of HPAM（1000 and 2000 mg/L） were assessed in the presence and absence of AFNPs at dosages of 200, 500 and 1000 mg/L. The nanocomposite was characterized by N2adsorption, Fourier-transformed infrared spectrophotometry（FTIR）, thermogravimetric analysis（TGA）, dynamic Light scattering（DLs）,and Zeta potential（ZP）. stability tests over time confirmed the positive effect of nanocomposite on increasing the thermochemical stability of polymer solutions. Results revealed that adding 0, 200, and 500 mg/L of nanocomposite to the polymeric solution at 1000 mg/L of HPAM, 10000 mg/L of NaCl, and 2000 mg/L of CaCl2·2H2O led to the viscosity reductions of 73.5%, 18%, and less than 1% after 7 days（70°C）, respectively. Nanocomposite at 200 mg/L reduces the polymer degradation in the presence of the two salts evaluated separately, i.e., 20% for 10000 mg/L of NaCl and 15% for 2000 mg/L of CaCl2·2H2O. The adsorption tests on AFNPs and siO2NPs concluded that AFNPs had higher adsorption of cations in comparison to siO2NPs and that greater adsorption of cations is related to a reduction in polymer degradation.

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