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Arsenic and chromium resistance mechanisms in the Micrococcus luteus group

Pedosphere 2023年第4期33卷 600-611页

作者： Ivan ARROYO-HERRERA Brenda romÁn-POnCE Rafael BUsTAMAnTE-BRITO Joseph GUEVARA-LUnA Erika Yanet TAPIA-GARCÍA Violeta LARIOs-sERRATO nannan ZHAnG Paulina EsTRADA-DE LOs sAnTOs En Tao WAnG María soledad VÁsQUEZ-MURRIETA Escuela nacional de Ciencias Biológicas Instituto Politécnico NacionalCiudad de México 11340(Mexico) Universidad Politécnica del Estado de Morelos Jiutepec 62550(Mexico) Centro de Ciencias Genómicas Universidad Autónoma de MéxicoCuernavaca 62210(Mexico) CAs Key Laboratory of Mountain Ecological Restoration and Bioresource Utilisation&Ecological Restoration and Biodiversity Conservation Key Laboratory of sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041(China)

High arsenic(As)and chromium(Cr)concentrations are currently receiving attention because of their negative effects on the environment and human *** inhabiting contaminated environments have developed resistance mechanisms against the toxicity of these ***,members of the bacterial genus Micrococcus have been isolated from different toxic metal-contaminated environments;however,knowledge concerning its resistance mechanisms to As and Cr toxicity remains *** luteus strains(An24,Mh,nE2TL6,and nE2TTs4)were isolated from the endosphere and soil of two heavy metal-contaminated sites in Mexico to identify differences in the resistance mechanisms by the *** *** strains were resistant to As(As^(3+)and As^(5+)),chromate,dichromate,cobalt,copper,nickel,and *** analysis indicated that the heavy metal-resistant strains(An24,Mh,nE2TL6,and nE2TTs4)could be assigned to the *** group and had more heavy metal-resistant genes(transporters,chaperones,and enzymes)compared to reference strains of the *** group,*** nCTC 2665^(T)and Micrococcus endophyticus JCM 16951^(T).The resistant bacteria were able to biotransform As^(3+)and As^(5+)through a carbon source-dependent *** biotransformation of As5+was potentially carried out in the cytoplasm through a thioredoxin-dependent pathway,which may be coupled with biosorption.A qualitative analysis of organic acids(OAs)identified a change in the OA profile of the metal-resistant strains that was As-or *** genomic and phenotypic findings suggest that the four *** group strains evaluated in the current study have developed resistance mechanisms that may enable their survival in contaminated sites.

关键词： arsenophore biosorption genome analysis heavy metal-resistant strain immobilization organic acid pollutant

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Unraveling one billion years of geological evolution of the southeastern Amazonia Craton from detrital zircon analyses

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Geoscience Frontiers 2022年第5期13卷 129-152页

作者： Camille Rossignol Paul Yves Jean Antonio Francesco narduzzi Eric siciliano Rego Lívia Teixeira romário Almeida de souza Janaína n.Ávila Marco A.L.silva Cristiano Lana Ricardo I.F.Trindade Pascal Philippot Departamento de Geofísica Instituto de AstronomiaGeofísica e Ciências Atmosféricas da Universidade de São PauloRua do Matão1226-Cidade UniversitáriaSão Paulo 05508-090Brazil Institut de Physique du Globe de Paris Universitéde ParisCNRSParisFrance Géosciences Montpellier Universitéde MontpellierCNRSUniversitédes AntillesMontpellierFrance Instituto de Geociências Universidade de São PauloRua do Lago562-Cidade UniversitáriaSão Paulo 05508-080Brazil Instituto de Geociências e Engenharias Universidade Federal do Sul e Sudeste do Pará–UNIFESSPAFolha 17Quadra 04Lote especialNova MarabáMarabáPará68505-080Brazil Research school of Earth sciences The Australian National University142 Mills RoadCanberraACT 2601Australia Applied Isotope Research Group Departamento de GeologiaEscola de MinasUniversidade Federal de Ouro PretoRua Diogo de Vasconcelos12235400-000 Ouro PretoMGBrazil

Despite representing one of the largest cratons on Earth,the early geological evolution of the Amazonia Craton remains poorly known due to relatively poor exposure and because younger metamorphic and tectonic events have obscured initial *** this study,we investigated the sedimentary archives of the Carajás Basin to unravel the early geological evolution of the southeastern Amazonia *** Carajás Basin contains sedimentary rocks that were deposited throughout a long period spanning more than one billion years from the Mesoarchean to the *** oldest archives preserved in this basin consist of a few ca.3.6 Ga detrital zircon grains showing that the geological roots of the Amazonia Craton were already formed by the *** the Paleoarchean or the early Mesoarchean(s Basin was large and rigid enough to sustain the formation and preservation of the Rio novo Group greenstone ***,during the neoarchean,at ca.2.7 Ga,the southeastern Amazonia Craton witnessed the emplacement of the Parauapebas Large Igneous Province(LIP)that probably covered a large part of the craton and was associated with the deposition of some of the world largest iron *** emplacement of this LIP immediately preceded a period of continental extension that formed a rift infilled first by iron formations followed by terrigenous *** major change of sedimentary regime might have been controlled by the regional tectonic evolution of the Amazonia Craton and its emergence above *** the Paleoproterozoic,at ca.2.1 Ga,the Rio Fresco Group,consisting of terrigenous sediments from the interior of the Amazonia Craton,was deposited in the Carajás *** that time,the Amazonian lithosphere could have either underwent thermal subsidence forming a large intracratonic basin or could have been deformed by long wavelength flexures that induced the formation of basins and swells throughout the craton under the influence of

关键词： Carajás Basin Chemical Abrasion–LA-ICP-Ms analyses Age distribution comparison Parauapebas Large Igneous Province Transamazonian orogeny

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Post-transplant biliary complications using liver grafts from deceased donors older than 70 years:Retrospective case-control study

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世界胃肠外科杂志:英文版(电子版) 2023年第8期15卷 1615-1628页

作者： Carlos Jimenez-romero Iago Justo-Alonso Pilar del Pozo-Elso Alberto Marcacuzco-Quinto Cristina Martín-Arriscado-Arroba Alejandro Manrique-Municio Jorge Calvo-Pulido Alvaro García-sesma Ricardo san román Oscar Caso-Maestro Unit of HPB surgery and Abdominal Organs Transplantation `12 de Octubre´University HospitalMadrid 28041Spain Clinical Research Unit(I+12) `12 de Octubre´University HospitalMadrid 28041Spain Department of Radiology `12 de Octubre´University HospitalMadrid 28041Spain

BACKGROUnD The shortage of liver grafts and subsequent waitlist mortality led us to expand the donor pool using liver grafts from older *** To determine the incidence,outcomes,and risk factors for biliary complications(BC)in liver transplantation(LT)using liver grafts from donors aged>70 *** Between January 1994 and December 31,2019,297 LTs were performed using donors older than 70 *** excluding 47 LT for several reasons,we divided 250 LTs into two groups,namely post-LT BC(n=21)and without BC(n=229).This retrospective case-control study compared both *** Choledocho-choledochostomy without T-tube was the most frequent technique(76.2%in the BC group vs 92.6%in the non-BC group).Twenty-one patients(8.4%)developed BC(13 anastomotic strictures,7 biliary leakages,and 1 nonanastomotic biliary stricture).nine patients underwent percutaneous balloon dilation and nine required a Rouxen-Y hepaticojejunostomy because of dilation *** incidence of post-LT complications(graft dysfunction,rejection,renal failure,and non-BC reoperations)was similar in both *** were no significant differences in the patient and graft survival between the ***,only three deaths were attributed to *** female donors were protective factors for BC,donor cardiac arrest was a risk *** The incidence of BC was relatively low on using liver grafts>70 *** could be managed in most cases by percutaneous dilation or Roux-en-Y hepaticojejunostomy,without significant differences in the patient or graft survival between the groups.

关键词： Older liver Liver transplant Biliary complications Biliary strictures septuagenarian donors Octogenarian donors

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Prognostic performance of an index based on lactic dehydrogenase and transaminases for patients with liver steatosis and COVID-19

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World Journal of Gastroenterology 2022年第37期28卷 5444-5456页

作者： Ricardo Ulises Macías-Rodríguez Alberto Adrián solís-Ortega Victoria J Ornelas-Arroyo Astrid Ruiz-Margáin Maria sarai González-Huezo nestor A Urdiales-Morán Berenice M román-Calleja Juan M Mayorquín-Aguilar JoséA González-Regueiro Alejandro Campos-Murguía Israel Vicente Toledo-Coronado Mónica Chapa-Ibargüengoitia Bernardo Valencia-Peña Carlos Fernando Martínez-Cabrera nayelli C Flores-García Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Department of Gastroenterology Centro Médico ISSEMYMToluca 52140Mexico Department of Radiology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico

BACKGROUnD Metabolic associated fatty liver disease(MAFLD)is associated with complications and mortality in patients with coronavirus disease 2019(COVID-19).However,there are no prognostic scores aimed to evaluate the risk of severe disease specifically in patients with MAFLD,despite its high *** dehydrogenase,aspartate aminotransferase and alanine aminotransferase have been used as markers of liver ***,we propose an index based on lactate dehydrogenase,aspartate aminotransferase and alanine aminotransferase for the prediction of complications and mortality in patients with MAFLD and *** To evaluate the prognostic performance of an index based on lactate dehydrogenase and transaminases(aspartate aminotransferase/alanine aminotransferase)in patients with COVID-19 and MAFLD[liver fibrosis and nutrition(LnF)-COVID-19 index].METHODs In this retrospective cohort study,two cohorts from two different tertiary centers were *** first was the derivation cohort to obtain the score cutoffs,and the second was the validation *** included hospitalized patients with severe COVID-19 and *** steatosis was evaluated by computed tomography *** under the receiver operating characteristic(ROC)curve analysis and survival analysis were *** In the derivation cohort,44.6%had MAFLD;ROC curve analysis yielded a LFn-COVID-19 index>1.67 as the best cutoff,with a sensitivity of 78%,specificity of 63%,negative predictive value of 91%and an area under the ROC curve of *** the multivariate analysis,the LFn-COVID-19 index>1.67 was independently associated with the development of acute kidney injury(odds ratio:1.8,95%confidence interval:1.3-2.5,Pntubation(odds ratio:1.9,95%confidence interval:1.4-2.4,Pnd death(odds ratio:2.86,95%confidence interval:1.6-4.5,Pn both *** LFn-COVID-19 index has a good performance to predict prognosis in patients with MAFLD and COVID-19,which

关键词： COVID-19 Metabolic associated fatty liver disease Lactate dehydrogenase Transaminases Prognosis nonalcoholic fatty liver disease

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丹麦舍饲散养自然通风奶牛舍的空气环境分析

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农业工程学报 2004年第5期20卷 231-236页

作者：李保明施正香 G Zhang 王朝元 H. B. rom s. Morsing J. s. strom 中国农业大学水利与土木工程学院 Danish Institute of Agricultural sciences

近年来,奶牛全舍饲散养工艺技术被欧美等国家越来越普遍地使用。全舍饲工艺中的舍内空气质量对奶牛的健康和生产性能影响很大,为较深入了解该工艺中热环境与其它有害气体的相应关系,通过对丹麦7个有代表性奶牛场进行温度、含湿量及几种... 详细信息

近年来,奶牛全舍饲散养工艺技术被欧美等国家越来越普遍地使用。全舍饲工艺中的舍内空气质量对奶牛的健康和生产性能影响很大,为较深入了解该工艺中热环境与其它有害气体的相应关系,通过对丹麦7个有代表性奶牛场进行温度、含湿量及几种主要有害气体的现场测试,对该工艺下舍内空气环境条件进行分析研究。结果表明:丹麦气候条件下全舍饲的自然通风牛舍,其舍内热湿状况受舍外温湿度及牛体本身的影响,在0～25℃范围内,舍温一般比舍外高1～3℃,舍外温度每升高1℃,舍内温度上升0.8℃,含湿量约增加1000mg/m3;舍内各环境因素之间具有显著的相关性,温度、含湿量的高低对nH3、CO2、CH4浓度均有影响;白天,由于自然通风量较大,这3种气体的平均浓度比夜间低20%左右。而n2O在整个测量过程中并未发现显著变化。

关键词：舍饲散养奶牛舍自然通风氨气

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synthesis and Characterization of Aluminum Alloys with Metal Oxides (CuO<sub>2sub>) Additions as Reinforcement

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Materials sciences and Applications 2023年第8期14卷 416-425页

作者： Reyna Anahí Falcón-Castrejón Jose Luis román-Zubillaga Rene Guardián-Tapia Lazaro Falcón-Franco Isaí Rosales-Cadena Center for Research in Engineering and Applied sciences Autonomous University of State of Morelos Cuernavaca Mexico Faculty of Metallurgy Autonomous University of Coahuila Coahuila Mexico

In this investigation, the addition of several amounts of metal oxide particles (CuO2) in Al matrix is carried out due to the need to improve the mechanical properties such as the ductility of aluminum for application... 详细信息

In this investigation, the addition of several amounts of metal oxide particles (CuOsub>2sub>) in Al matrix is carried out due to the need to improve the mechanical properties such as the ductility of aluminum for applications in the electrical sector. samples were obtained by means of a stirring casting process. From the results of the microstructural characterization, it was observed that the metallic oxides induce the modification of the dendritic structure and grain refinement. X-ray diffraction characterization mainly shows the formation of Alsub>2sub>CuOsub>4sub>, Alsub>2sub>Osub>3sub> and CuO compounds. Mechanical properties showed that the different thermal treatments resulted in an improved hardness, from 30 kg/mmsup>2sup> for the un-reinforced sample to 90 kg/mmsup>2sup> for reinforced samples. The addition of metallic oxides in the Al matrix produces an improved electrical conductivity specifically in sample with 0.50 g of CuOsub>2sub> additions.

关键词： Electrical Properties Oxides Additions soft Alloys Mechanical Properties

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studies and Mechanism of Olefination Reaction in Aryl-Enolates with Paraformaldehyde

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International Journal of Organic Chemistry 2019年第1期9卷 10-22页

作者： Jonathan román Valdéz-Camacho José Domingo Rivera-Ramírez Jaime Escalante Centro de Investigación en Ciencias-IICBA Universidad Autónoma del Estado de Morelos Cuernavaca México Centro Universitario de Ciencias Exactas e Ingenierías Departamento de Química Universidad de Guadalajara Guadalaja México

A simple, efficient and low-cost methodology for the synthesis of α-aryl-α,β-unsaturated esters using paraformaldehyde as a source of carbon was developed. Factors that control reaction yields such as temperature, concentration and reaction time were evaluated. A mechanism is proposed based on experimental structures of the intermediates.

关键词： Olefination Paraformaldehyde α-Aryl-α β-Unsaturated Ester α-Methylenation

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苹果树冠生长发育所需光的临界值

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中国农业气象 1992年第6期13卷 50-55页

作者： Curt R.rom 傅友

了解树冠的生长发育特性对果树栽培管理是很重要的.树冠自然的承载着果品产量.树冠的叶幕可截获光能并制造成碳水化合物,用于树体生长发育及代谢活动;冠层光照分布情况影响果实着色及品质、树冠发育速率,在形成期还影响病虫危害及其防治。

关键词：苹果树树冠生长发育光照

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Application of Different Kinetic Methods to the Thermogravimetric Analysis of Kenaf（Hibiscus Cannabinus）

Application of Different Kinetic Methods to the Thermogravim...

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The 18th European Biomass Conference and Exhibition

作者： González J.F. Encinar J.M. román s. Rayo M.C.

The objective of this work was to investigate the thermal decomposition process of kenaf（Hibiscus cannabinus） and to determine their activation energy values associated through a dynamic thermogravimetric analysis by model-free methods. The pyrolysis of kenaf has been carried out by means of thermogravimetric analysis in Ar atmosphere. Four heating rates were used（5-10-20-40 K min-1） up to a final temperature of 700 oC. The activation energy values were calculated as a function of the extent of conversion during the pyrolysis process by means of Friedman, Kissinger-Akahira-sunose and Flynn–Wall–Ozawa isoconversional methods. The results showed that there are small differences between the activation energy values obtained from the three methods. Also, a relationship between the activation energy and the conversion reached was found.

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Changes in balance ability,power output,and stretch-shortening cycle utilisation after two high-intensity intermittent training protocols in endurance runners

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Journal of sport and Health science 2016年第4期5卷 430-436页

作者： Felipe García-Pinillos Juan A.Párraga-Montilla Víctor M.soto-Hermoso Pedro A.Latorre-román Department of Corporal Expression University of Jaen Department of sports sciences University of Granada

Purpose：This study aimed to describe the acute effects of 2 different high-intensity intermittent trainings（HIITs） on postural control,countermovement jump（CMJ）,squat jump（sJ）,and stretch-shortening cycle（ssC） utilisation,and to compare the changes induced by both protocols in those variables in endurance ***：Eighteen recreationally trained endurance runners participated in this study and were tested on 2 occasions：10 runs of 400 m with 90 s recovery between running bouts（10×400 m）,and 40 runs of 100 m with 30 s recovery between runs（40 × 100 m）.Heart rate was monitored during both HIITs;blood lactate accumulation and rate of perceived exertion were recorded after both *** jump ability（CMJ and sJ） and ssC together with postural control were also controlled during both ***：Repeated measures analysis revealed a significan improvement in CMJ and sJ during 10 × 400 m（p〈0.05）,whilst no significan changes were observed during 40×100 *** related to ssC did not experience significan changes during any of the *** for postural control,no significan changes were observed in the 40×100 m protocol,whilst significan impairments were observed during the 10×400 m protocol（p〈0.05）.Conclusion：A protocol with a higher number of shorter runs（40×100 m） induced different changes in those neuromuscular parameters than those with fewer and longer runs（10×400 m）.Whereas the 40×100 m protocol did not cause any significan changes in vertical jump ability,postural control or ssC utilisation,the 10×400 m protocol impaired postural control and caused improvements in vertical jumping tests.

关键词： Long-distance runner Postural control Reactive strength Training prescription Vertical jump

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