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Implementation of the Acclimatization Process for Ginger Plantlets (Zingiber officinale Roscoe)

Open Journal of Applied Sciences 2024年第8期14卷 2236-2248页

作者： Florentin Danton Kango Gilles Habib Todjro Cacaï Serge Sètondji Houédjissin jerome anani hounguè Jaures Riwan Uriel Kouke David Dègbé Allotchémè Corneille Ahanhanzo Central Laboratory of Plant Biotechnology and Plant Breeding Department of Genetic and Biotechnology Faculty of Science and Technology University of Abomey-Calavi Cotonou Benin

Ginger (Zingiber officinale Roscoe) is an important culinary and medicinal spice but is rarely cultivated due to the unavailability of seeds. Given the difficulties in adapting to plantlets produced in natural environments, it is important to analyze the survival conditions of ginger plantlets. For this reason, we varied the incubation temperature and humidity as well as the substrate during the weaning phase. Then, we varied the nutrients contained in the watering solution during the hardening phase. The statistical analysis showed that physical factors and substrates significantly influenced (p < 0.0001) plantlet survival. Nutrient solutions significantly influenced the phylogenesis, rhizogenesis, and height growth of the plantlets. The suitable physical factors for good development of plantlets are a temperature of 26.54˚C and a humidity of 96.16%. The 1C2T2TC substrate (1 Compost + 2 Soil + 2 Coconut Peat) had a significant survival rate of approximately 92.5%. During hardening, the Plantzym solution promoted good growth in terms of plantlet height (0.6 cm) and good development of roots (30 roots) and leaves (03 leaves). This work will make it possible to develop a technical seed production sheet for better development of ginger cultivation in Benin.

关键词： Acclimatization Zingiber officinale Weaning Nutrient Solutions Substrates

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Effects of Non-Conventional Substrate and NPK Fertilizer on Cassava Plantlets Acclimatization for Plant Material Production

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Agricultural Sciences 2021年第10期12卷 1058-1069页

作者： Gilles Habib Todjro Cacaï Corneille Ahanhanzo Dèwanou Kant David Ahoya Serge Sètondji Houédjissin jerome anani houngue Central Laboratory of Plant Biotechnology and Plant Breeding Department of Genetics and Biotechnology Faculty of Science and Technology University of Abomey-Calavi Cotonou Benin

The adaptation of cassava plantlets to natural conditions remains the main obstacle in the process of free virus plantlets production. In this study, cassava plantlets acclimatization was assessed by pre-acclimatization, height of acclimated plantlets, substrate, and nutrient watering solution. The results revealed that pre-acclimatization gave a high survival rate (61.9%) whereas the direct tunnel acclimatization was conducted to low rate (27.31%) of plantlets. Furthermore, the height of plantlets influenced significantly (p < 0.05) their survival rate with best survival rate observed on plantlet height from 1 cm to 5 cm. There was no significant difference between the substrates used and watering solutions. However, the Sawdust substrate gave a high survival rate (46.67%) and better phylogenesis (1.8) in the variety RB 89509. Likewise, plantlets treated with NPK nutrient watering solutions (20-10-10) had a better survival rate (48.57%), better phylogenesis (2.125) with the variety RB 89509 and better growth in height (1.18 cm) with the variety BF92/0267. These findings constitute a database for the establishment of the technical itinerary for the acclimatization of cassava plantlets.

关键词： Manihot esculenta Plantlets Pre-Acclimatization Substrates Nutrient Solution NPK

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Plant Regeneration through Indirect Organogenesis in Two Cultivars of Pineapple (Ananas comosus L.)

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Open Journal of Applied Sciences 2023年第7期13卷 1039-1058页

作者： Gilles H. T. Cacaï Brunith A. M. Ahokpossi Serge S. Houédjissin jerome anani houngue Bienvenu T. Badou Corneille Ahanhanzo Central Laboratory of Plant Biotechnology and Plant Breeding Department of Genetics and Biotechnology Faculty of Science and Technique University of Abomey-Calavi Abomey-Calavi Benin Beninese Center of Scientific Research and Innovation Cotonou Benin

Unavailability of performant planting material of pineapple constitutes a major problem of its cultivation in Africa. For this purpose, indirect organogenesis technique is used to evaluate the in vitro responses of two cultivars of pineapple during the explant’s regeneration. Calli were induced from crown leaf and plantlets leaf of “Smooth Cayenne” and “Sugarloaf cultivars”. Murashige and Skoog medium with vitamins B5 supplemented with different growth regulators combinations were used. BAP and/or 2,4-D have been added to base medium for calli cells’ differentiation while BAP and GA3 have been added for plant elongation. The results indicated that explants from regenerated plantlets leaves cultivated on MS supplemented with copper (II) sulphate 5-hydrate concentrations incorporated had significant (p < 0.0001) influence on callus induction in pineapple cultivars. Likewise, MS medium with NAA (0.5 mg/l) + BAP (1 mg/l) had a highly significant influence with 8.8 differentiated Calli. Also, MS medium supplemented with BAP (3 mg/l) + GA3 (2 mg/l) for the “smooth Cayenne” had significantly influenced (p < 0.0001) Calli regeneration with a high rate of 55.25% plantlets. MS medium containing 0.5 mg/l of NAA + 0 mg/l IBA produced a high number of roots in Sugarloaf whereas the medium containing 1.5 mg/l NAA + 0.5 mg/l (IBA) produced high number of roots in smooth Cayenne. We have established an efficient and reproducible protocol for mass propagation and genetic transformation of pineapple though indirect organogenesis. This protocol may be used in genetics engineering studies for pineapple breeding.

关键词： Callus Induction Plant Growth Regulators Stomata Structure Rhizogenesis Pineapple

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Plant Regeneration through Indirect Organogenesis in Two Cultivars of Pineapple (Ananas comosus L.)

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Open Journal of Applied Sciences（应用科学） 2023年第7期

作者： Gilles H.T.Cacaï Brunith A.M.Ahokpossi Serge S.Houédjissin jerome anani houngue Bienvenu T.Badou Corneille Ahanhanzo

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