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Modeling gut neuro-epithelial connections in a novel microfluidic device

Microsystems & Nanoengineering 2023年第6期9卷 235-246页

作者： Jose M.de Hoyos-Vega Xi Yu Alan M.Gonzalez-Suarez Sisi Chen Arnaldo Mercado-Perez Eugene Krueger jeric hernandez Yaroslav Fedyshyn Brooke R.Druliner David R.Linden Arthur Beyder Alexander Revzin Department of Physiology and Biomedical Engineering Mayo ClinicRochesterMNUSA Enteric Neuroscience Program(ENSP) Mayo ClinicRochesterMNUSA Division of Gastroenterology and Hepatology Department of MedicineMayo ClinicRochesterMNUSA

The intestinal lumen is filled with diverse chemical and physical stimuli.Intestinal epithelial cells sense these stimuli and signal to enteric neurons which coordinate a range of physiologic processes required for normal digestive tract function.Yet,the neuro-epithelial connections remain poorly resolved,in part because the tools for orchestrating interactions between these cellular compartments are lacking.We describe the development of a two-compartment microfluidic device for co-culturing enteric neurons with intestinal epithelial cells.The device contains epithelial and neuronal compartments connected by microgrooves.The epithelial compartment was designed for cell seeding via injection and confinement of intestinal epithelial cells derived from human intestinal organoids.We demonstrated that organoids planarized effectively and retained epithelial phenotype for over a week.In the second chamber we dissociated and cultured intestinal myenteric neurons including intrinsic primary afferent neurons(IPANs)from transgenic mice that expressed the fluorescent protein tdTomato.IPANs extended projections into microgrooves,surrounded and frequently made contacts with epithelial cells.The density and directionality of neuronal projections were enhanced by the presence of epithelial cells in the adjacent compartment.Our microfluidic device represents a platform that may,in the future,be used to dissect structure and function of neuro-epithelial connections in the gut and other organs(skin,lung,bladder,and others)in health and disease.

关键词： fluid bladder organs

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Healthcare Professionals’ Adherence to Contact Precautions at a Maternal and Child Teaching Hospital

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Open Journal of Nursing 2023年第12期13卷 839-854页

作者： Lucas Eduardo Luizão Marli de Carvalho jericó Angela Silveira Gagliardo Calil Viviane Decicera Colombo Oliveira Alexandre Lins Werneck Ingrid de Campos Truzzi Eliana Ofelia Lapa Rodriguez Maria Rita Rodrigues Vieira Pedro Paulo de Carvalho jericó Medical School in Sã o Jose do Rio Preto S&#227o Jose do Rio Preto Brazil Federal University of Sergipe Sergipe Brazil

Background: Healthcare-associated infections affect hundreds of millions of patients worldwide. Children have greater susceptibility to healthcare-associated infections due to the immaturity of their immune system. Contact precautions aim to promote safety, protection and prevention of contamination. Thus, the objective of this study was to verify adherence to contact precaution measures, as well as compliance to the use of personal protective equipment. Methods: This cross-sectional observational study was carried out from July to October 2019 using a checklist to evaluate health professionals’ adherence to hand hygiene procedures and the use of gloves and surgical gowns when assisting children on contact precautions. Results: A total of 941 observations were carried out in a total of 300.532 hours. Hand hygiene was performed before and after contact with the patient in 58.84% and 75.09% of the cases, respectively and a surgical gown was used in 86.40% of the cases. The use of gloves was the variable most adhered to by professionals (87.57%). Intensive care unit professionals were the care workers who most complied with the regulation regarding hand hygiene after contact with the patient (p = 0.009) and the use of the surgical gown (p < 0.001). The correct hand hygiene technique was the recommendation with least adherence. Non-compliance to the hand hygiene technique was statistically significant among intensive care unit professionals (p = 0.002). Conclusions: Adherence to hand hygiene before contact with the patient and compliance with the hand hygiene technique were neglected by most professionals. However, there was good adherence to the use of surgical gloves and gowns, as well as high compliance to the techniques of removing these items. .

关键词： Hospital Infection Universal Precautions Patient Safety Pediatric Nursing Isolation of Patients Microbial Drug Resistance

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Contact Precaution: Acceptance of Health Professionals to Teaching Hospital

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Open Journal of Nursing 2020年第5期10卷 551-562页

作者： Hercília Oliveira dos Santos Marli de Carvalho jericó Viviane Decicera Colombo Oliveira Geraldo Magela de Faria Junior Patrícia de Carvalho jericó Pedro Paulo de Carvalho jericó Ingrid Gomes Campos Truzzi Angela Silveira Gagliardo Calil Faculdade de Medicina de São José do Rio Preto (FAMERP) São José do Rio Preto Brazil Pontifícia Universidade Católica do Rio Grande do Sul Porto Alegre Brazil Pontifícia Universidade Católica do Paraná Curitiba Brazil

The adoption by health professionals to the practice of patient isolation is a decision that reduces patients’ risks of occupational exposure to potentially contaminated biological material and prevents nosocomial infections. Verify the compliance of health professionals to the practices of contact precautions in patients colonized by microorganisms in a teaching hospital. This was a prospective observational cohort study in a special-sized hospital located in southeastern Brazil. The observation of health professionals in delivery of health care to patients with contact precaution was performed regarding hand hygiene, use of overcoat, glove, and mask. We carried out 1502 observations involving the following professional categories: nursing technicians and/or nursing assistants (n = 1028;68.4%), nurses (n = 200;13.3%), physical therapists (n = 185;12.3%), and physicians (n = 89;5.9%), totaling 971.8 hours. Regarding the level of compliance of professionals to contact precaution practices with hand hygiene after the procedures, the use of overcoats, gloves, and masks, all were statistically significant (p Klebsiella pneumoniae was the most positive pathogen identified in laboratory tests. This study provided the mapping of the compliance of health professionals to the practices of contact precautions in order to support a safer management of patient care reducing the risks of Healthcare-Associated Infections (HAI). This study also showed that health professionals are using practice management to deal with their behavior to protect their health.

关键词： Universal Precautions Infection Control Services Hospital Patient Isolation Drug Resistance Microbial Quality Indicators Health Care Process Assessment (Health Care)

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渥太华声明:人体医疗类干预临床试验研究方案信息与结果的国际注册原则(第Ⅰ部分)

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中国循证儿科杂志 2006年第2期1卷 155-159页

作者： Karmela Krleza-jeric An-Wen Chan Kay Dickersin Ida Sim Jeremy Grimshaw Christian Gluud 刘建平(翻译) 张晶(翻译) 陈君超(翻译) 随机对照试验中心加拿大卫生研究学院多伦多大学医学系布朗大学社区健康系加利福尼亚大学医学系临床流行病学项目渥太华卫生研究学院欧洲临床研究基础设施网络哥本哈根临床试验中心临床干预研究中心 Cochrane肝胆疾病组哥本哈根大学医院(丹麦国家医院)丹麦哥本哈根北京中医药大学循证医学中心上海中医药大学中医循证医学中心上海201203

1目的渥太华声明旨在为临床试验注册建立国际认可的标准（第Ⅰ部分），以及根据这一标准提议的操作方案（第Ⅱ部分）。

关键词：临床试验研究国际注册信息医疗国际认可标准

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