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Emergence and Seedling Characteristics of Maize Native to the Southwestern US

American Journal of Plant Sciences 2017年第6期8卷 1304-1318页

作者： jennifer M. Bousselot deborah Muenchrath Allen d. Knapp Jean d. Reeder department of Horticulture and landscape Architecture Colorado State University and Colorado Native Plant Society Colorado USA department of Agronomy Iowa State University Iowa USA USdA-ARS Windsor Colorado USA

locally adapted maize landraces, which are associated with Native American groups, were traditionally planted deeply, reportedly up to 45 cm deep. Crop resources such as these should be evaluated for possible use in future sustainable farming practices. Cold temperatures often delay maize (Zea mays l.) planting in the Corn Belt, possibly reducing yield potential, and spring frost and hail can damage early plantings. If producers could plant deeper and earlier in the spring, the planting season period could be extended and the potential for frost damage reduced because the growing point would be insulated below the soil surface for a longer period of time. The emergence capabilities of eleven Native American landraces were evaluated at various planting depths and compared to one Corn Belt line, BSSS-53. Emergence from depths between 5 and 45 cm was evaluated in a growth chamber study. Seedling dry matter partitioning and morphological characteristics were also examined. A field study was then performed to further test those landraces that successfully emerged (≥ 75%) from the 25 cm depth in the growth chamber. Results indicate that some of the evaluated Native American landraces have a greater capacity to emerge from depth than BSSS-53. Emergence capacity was not related to initial seed weight. Mesocotyl elongation largely accounted for successful emergence from greater planting depths. The landraces partitioned relatively more dry matter to roots than shoots compared to BSSS-53. These results suggest that several of these Native American landraces may be useful for the development of maize varieties more tolerant to deep planting.

关键词： Native American Maize landraces Emergence from depth Mesocotyl length Radicle

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Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation

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world Journal of Gastroenterology 2014年第21期20卷 6560-6572页

作者： jennifer Raisch Emmanuel Buc Mathilde Bonnet Pierre Sauvanet Emilie Vazeille Amélie de Vallée Pierre déchelotte Claude darcha denis Pezet Richard Bonnet Marie-Agnès Bringer Arlette darfeuille-Michaud Clermont Université UMR1071 Inserm/Ud’Auvergne and INRA USC201863000 Clermont-FerrandFrance Centre Hospitalier Universitaire 63000 Clermont-FerrandFrance

AIM: To provide further insight into the characterization of mucosa-associated Escherichia coli (E. coli) isolated from the colonic mucosa of cancer patients.

关键词： B2 Escherichia coli Carcinoembryonic antigen-related cell adhesion molecule 6 Cell proliferation Colon cancer Polyketide synthase genomic island

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体外冲击波疗法对膝关节骨性关节炎大鼠滑膜和软骨组织炎症因子的影响及机制

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贵州医科大学学报 2024年第1期49卷 48-55页

作者：杨兴月黄圆月安松松毛冬梅黄媛馨杨俊龙于子龙秦乐王林沃春新贵州医科大学麻醉学院贵州贵阳550004 贵州医科大学附属医院疼痛科贵州贵阳550004

目的探讨体外冲击波疗法(ESWT)对膝关节骨性关节炎(KOA)大鼠滑膜及软骨组织炎症因子的影响及机制。方法 30只雄性Sprague dawley(Sd)大鼠随机均分为对照组、模型组及冲击波组,对照组大鼠右侧关节腔注射50μl生理盐水,模型组与冲击波组... 详细信息

目的探讨体外冲击波疗法(ESWT)对膝关节骨性关节炎(KOA)大鼠滑膜及软骨组织炎症因子的影响及机制。方法 30只雄性Sprague dawley(Sd)大鼠随机均分为对照组、模型组及冲击波组,对照组大鼠右侧关节腔注射50μl生理盐水,模型组与冲击波组大鼠右侧膝关节腔注射碘乙酸钠(MIA)2 mg/50μl构建KOA模型,均干预14 d;干预结束后对照组、模型组大鼠行冲击波声音刺激,冲击波组大鼠予ESWT治疗,1次/周、共4周;分别于术前和术后第3、7、14、21、28、35及42天,采用热刺痛仪和足底刺痛仪检测各组大鼠右后爪的热缩爪潜伏期(PWTl)和足底机械刺激缩足阈值(MWT);术后第42天,各组大鼠给予10%水合氯醛腹腔注射麻醉,行右膝关节X线检查,然后处死、取右侧膝关节,采用免疫组织化学法(IHC)检测各组大鼠膝关节滑膜和软骨组织白细胞介素-1β (Il-1β)、Il-6及肿瘤坏死因子-α(TNF-α)的表达水平。结果模型组和冲击波组大鼠术后第3~42天的PWTl均较对照组缩短(Pl较模型组延长(Pl-1β及Il-6水平比较,对照组l-1β、Il-6及TNF-α的表达有关。

关键词：软骨滑膜肿瘤坏死因子α 白细胞介素1β 白细胞介素6 体外冲击波疗法膝关节骨性关节炎

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克隆测序确认HlA新等位基因B~*3712^(★○)

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中国组织工程研究与临床康复 2007年第50期11卷 10131-10133页

作者：张伯伟邢培清赵磊 Ana lazaro jennifer Ng 河南省红十字血液中心河南省郑州市450012 美国海军骨髓库洛克维尔马里兰州美国20852

目的:由于技术原理的限制,目前尚不能对所有的HlA等位基因进行严格的区分,特别是以往没有发现的新基因序列只能通过测序的方法解决,然而,当遇到等位基因杂合时,测序给出的结果仍然无法确认新的序列改变发生在等位基因的哪一侧,这时需要... 详细信息

目的:由于技术原理的限制,目前尚不能对所有的HlA等位基因进行严格的区分,特别是以往没有发现的新基因序列只能通过测序的方法解决,然而,当遇到等位基因杂合时,测序给出的结果仍然无法确认新的序列改变发生在等位基因的哪一侧,这时需要用分子生物学方法分离杂合子然后进行测序才能确定新的基因序列。采用基因克隆方法确认HlA新等位基因。方法:实验于2006-01/05在河南省红十字血液中心HlA实验室,美国海军骨髓库HlA实验室完。成造血干细胞血样由中华骨髓库提供。采用荧光微珠HlA分型方法对中华骨髓库捐献者血样进行HlA分型检测,无法给出确切结果的摸棱两可结果标本用基因克隆(TOPO TA Cloning)、dNA测序的方法确认新的HlA基因序列。结果:通过克隆分离杂台等位基因,再进行测序确认发现新的序列与B~*3709相比,出现4个核苷酸改变:1.355nt C>A,2.363nt C>G,3.412ntG>A,4 477nt C>G,而且均发生在HlA-B基因外显子3(exon 3)。4处改变引起氨基酸编码改变:①编码95 CTC>ATC,氨基酸改变l>I(亮氨酸>异亮氨酸)。②97 AGC>AGG,S>R(丝氨酸>精氨酸)。③114 GAC>AAC d>N(天门冬氨酸>天冬酰胺)。④135 GCC>GCG A=A无氨基酸改变。结论:①新的基因序列已经在GenBnak注册,被WHO的HlA因子命名委员会得到正式命名为HlA-B~*3712基因。②基因克隆是确认HlA新基因的根本方法。

关键词： HlA新基因基因克隆 dNA测序

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The Plastid-localized NAd-dependent Malate dehydrogenase Is Crucial for Energy Homeostasis in developing Arabidopsis thaliana Seeds

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Molecular Plant 2014年第1期7卷 170-186页

作者： jennifer Selinski Nicolas Konig Benedikt Wellmeyer Guy T. Hanke Vera linke H. Ekkehard Neuhaus Renate Scheibe department of Plant Physiology FB 5 University of Osnabrueck D-49069 Osnabrueck Germany department of Plant Physiology FB Biology TU Kaiserslautern D-67663 Kaiserslautern Germany

In the absence of photosynthesis, ATP is imported into chloroplasts and non-green plastids by ATP/AdP transporters or formed during glycolysis, the latter requiring continuous regeneration of NAd＋, supplied by the plastidial isoform of NAd-MdH. during screening for T-dNA insertion mutants in the plNAd-MdH gene of Arabidopsis, only heterozygous plants could be isolated and homozygous knockout mutants grew only after complementation. These heterozygous plants show higher transcript levels of an alternative NAd＋-regenerating enzyme, NAdH-GOGAT, and, remarkably, improved growth when ammonium is the sole N-source. In situ hybridization and GUS-histochemical stain- ing revealed that plNAd-MdH was particularly abundant in male and female gametophytes. Knockout plNAd-MdH pollen exhibit impaired tube growth in vitro, which can be overcome by adding the substrates of NAdH-GOGAT. In vivo, knockout pollen is able to fertilize the egg cell. Young siliques of selfed heterozygous plants contain both green and white seeds corresponding to wild-type/heterozygous （green） and homozygous knockout mutants （white） in a （1：2）：1 ratio. Embryos of the homozygous knockout seeds only reached the globular stage, did not green, and developed to tiny wrinkled seeds. Complementation with the gene under the native promoter rescued this defect, and all seeds developed as wild-type. This suggests that a blocked major physiological process in plNAd-MdH mutants stops both embryo and endosperm development, thus avoiding assimilate investment in compromised offspring.

关键词： malate valve plastidial glycolysis ammonium assimilation NAdH-GOGAT redox-balance embryo lethality.

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Refractive outcomes among glaucoma patients undergoing phacoemulsification cataract extraction with and without Kahook dual Blade goniotomy

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Eye and Vision 2019年第1期6卷 236-241页

作者： Erin G.Sieck Cara E.Capitena Young Rebecca S.Epstein Jeffrey R.SooHoo Mina B.Pantcheva jennifer l.Patnaik Anne M.lynch Malik Y.Kahook leonard K.Seibold department of Ophthalmology University of Colorado School of Medicine1675 Aurora Court F731AuroraCO 80045USA

Background:Glaucoma patients undergoing phacoemulsification alone have a higher rate of refractive surprise compared to patients without *** risk is further increased with combined filtering ***,there are few and conflicting reports on the effect of combined phacoemulsification and micro-invasive glaucoma surgery(MIGS).Here,we look at refractive outcomes of glaucoma patients undergoing phacoemulsification with and without Kahook dual Blade(KdB)***:Retrospective chart review of 385 glaucomatous eyes of 281 patients,which underwent either phacoemulsification alone(n=309)or phacoemulsification with KdB goniotomy(n=76,phaco-KdB)at the University of *** main outcome was refractive surprise defined as the difference in target and postoperative refraction spherical equivalent greater than±0.5 diopter(d).Results:Refractive surprise greater than±0.5 d occurred in 26.3%of eyes in the phaco-KdB group and 36.2%in the phacoemulsification group(p=0.11).Refractive surprise greater than±1.0 d occurred in 6.6%for the phacoKdB group and 9.7%for the phacoemulsification group(p=0.08).There was no significant difference in risk of refractive surprise when pre-operative IOP,axial length,keratometry or performance of KdB goniotomy were assessed in univariate ***:There was no difference between refractive outcomes of glaucomatous patients undergoing phacoemulsification with or without KdB goniotomy.

关键词： Kahook dual Blade goniotomy refractive surprise glaucoma cataract

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Reimagining large river management using the Resist-Accept-direct(RAd)framework in the Upper Mississippi River

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Ecological Processes 2023年第1期12卷 645-664页

作者： Nicole K.Ward Abigail J.lynch Erik A.Beever Joshua Booker Kristen l.Bouska Holly Embke Jeffrey N.Houser John F.Kocik Joshua Kocik david J.lawrence Mary Grace lemon doug limpinsel Madeline R.Magee Bryan M.Maitland Owen McKenna Andrew Meier John M.Morton Jeffrey d.Muehlbauer Robert Newman devon C.Oliver Heidi M.Rantala Greg G.Sass Aaron Shultz laura M.Thompson jennifer l.Wilkening Minnesota department of Natural Resources Lake CityMN 55041USA U.S.Geological Survey National Climate Adaptation Science CenterRestonVA 22041USA U.S.Geological Survey Northern Rocky Mountain Science CenterBozemanMT 59715USA department of Ecology Montana State UniversityBozemanMT 59715USA U.S.Fish and Wildlife Service National Wildlife Refuge SystemOak HarborOH 43449USA U.S.Geological Survey Upper Midwest Environmental Sciences CenterLa CrosseWI 54603USA U.S.Geological Survey Midwest Climate Adaptation Science CenterSt.PaulMN 55108USA NOAA NEFSC OronoME 04473USA University of Nebraska-lincoln LincolnNE 68503USA National Park Service Climate Change Response ProgramFort CollinsCO 80525USA U.S.Fish and Wild-life Service National Wildlife Refuge SystemBloomingtonMN 55437USA Habitat Conservation division NOAAAlaska RegionAnchorageAK 99513USA Wisconsin department of Natural Resources MadisonWI 53703USA Aquatic Sciences Center Center for LimnologyUW-MadisonMadisonWI 53706USA U.S.Geological Survey Northern Prairie Wildlife Research Center and Midwest Climate Adaptation Science CenterJamestownND 58401USA U.S.Army Corps of Engineers La CrescentMN 55947USA Alaska Wildlife Alliance AnchorageAK 99520USA U.S.Geological Survey Alaska Cooperative Fish and Wildlife Research UnitUniversity of Alaska FairbanksFairbanksAK 99775USA University of North dakota Grand ForksND 58202USA Minnesota department of Natural Resources DuluthMN 55804USA Wisconsin department of Natural Resources Boulder JunctionWI 54512USA Great lakes Indian Fish and Wildlife Commission(GlIFWC) OdanahWI 54861USA U.S.Geological Survey National Climate Adaptation Science Centerand School of Natural ResourcesUniversity of TennesseeKnoxvilleTN 37996USA U.S.Fish and Wildlife Service National Wildlife Refuge SystemFort CollinsCO 80526USA

Background large-river decision-makers are charged with maintaining diverse ecosystem services through unprec-edented social-ecological transformations as climate change and other global stressors *** interconnected,dendritic habitats of rivers,which often demarcate jurisdictional boundaries,generate complex management ***,we explore how the Resist–Accept–direct(RAd)framework may enhance large-river management by promoting coordinated and deliberate responses to social-ecological trajectories of *** RAd frame-work identifies the full decision space of potential management approaches,wherein managers may resist change to maintain historical conditions,accept change toward different conditions,or direct change to a specified future with novel *** the Upper Mississippi River System,managers are facing social-ecological transformations from more frequent and extreme high-water *** illustrate how RAd-informed basin-,reach-,and site-scale decisions could:(1)provide cross-spatial scale framing;(2)open the entire decision space of potential management approaches;and(3)enhance coordinated inter-jurisdictional management in response to the trajectory of the Upper Mississippi River *** The RAd framework helps identify plausible long-term trajectories in different reaches(or subbasins)of the river and how the associated social-ecological transformations could be managed by altering site-scale *** reach-scale objectives may reprioritize how,where,and when site conditions could be altered to contribute to the basin goal,given the basin’s plausible trajectories of change(e.g.,by coordinating action across sites to alter habitat connectivity,diversity,and redundancy in the river mosaic).Conclusions When faced with long-term systemic transformations(e.g.,>50 years),the RAd framework helps explicitly consider whether or when the basin vision or goals may no longer be achievable,and direct options may open y

关键词： Ecosystem Management Transformation Social-ecological system Anthropocene Climate change Basin planning Cross-scale interactions

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网络融资模式下林业小微企业信用风险管控机制研究

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西部林业科学 2022年第6期51卷 164-171页

作者：周茜吴小我吕淼虹浙江农林大学暨阳学院浙江诸暨311800 塔斯马尼亚大学科学与工程学院澳大利亚塔斯马尼亚霍巴特7001

林业小微企业对丰富林产品市场、盘活林业资源、促进我国经济发展、扩大就业等方面做出了重要的贡献,但是林业小微企业存在资金缺乏、融资难等问题。网络融资模式是为林业小微企业提供贷款的一种新型网络融资模式,但其存在着一定信用风... 详细信息

林业小微企业对丰富林产品市场、盘活林业资源、促进我国经济发展、扩大就业等方面做出了重要的贡献,但是林业小微企业存在资金缺乏、融资难等问题。网络融资模式是为林业小微企业提供贷款的一种新型网络融资模式,但其存在着一定信用风险。针对网络融资模式下的林业小微企业主要信用风险因素,通过博弈论、信息经济建模等方法,提出科学的林业小微企业信用风险评价机制、基于演化稳定策略(ESS)的网络融资模式下信用风险管控机制、基于网络联保交易的信用风险管控机制、基于区块链技术下网络融资创新模式的信用风险管控机制等来控制林业小微企业的信用风险,从而提升网络融资模式下林业小微企业融资水平以及信用风险管控能力。

关键词：林业小微企业网络融资模式信用风险区块链

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down-regulation of dopamine d2 receptor associates with impaired reversal learning induced by morphine withdrawal

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中国药理学与毒理学杂志 2018年第9期32卷 717-717页

作者： lI Fei HE li lI Jin jennifer l WHISTlER Beijing Institute of Pharmacology and Toxicology Beijing 100850 China department of Neurology University of California San Francisco San Francisco CA 94143 USA

OBJECTIVE Cognitive inflexibility plays a critical role in the compulsive drug taking,a central characteristic of drug addictions,yet its underlying neurochemical mechanisms are not well *** present study examined the impact of morphine withdrawal on reversal *** Reversal learning was tested in a four-choices digging *** brain tissues were harvested 2 h after the behavioral experiment for the further *** We found that after long-term abstinence for a month from chronic morphine exposure,mice exhibited a profound reversal learning *** further found that dopamine d2 receptor(d2R)system in the frontal-striatal circuit is significantly down-regulated,at both receptor and downstream signals *** pharmacological experiments demonstrated that aripiprazole,a d2R partial agonist,prevented the d2R downregulation and rescued the reversal learning *** Together,our findings provide valuable insights into the causal relationship between d2R system in the frontal-striatal circuit and the cognitive inflexibility caused by abused drugs and offer a promising possibility of an effective therapeutic intervention for drug addictions.

关键词： reversal learning dopamine d2 receptor morphine cognitive inflexibility

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The effect of acute exercise on cognitive performance in children with and without AdHd

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Journal of Sport and Health Science 2015年第1期4卷 97-104页

作者： Aaron T.Piepmeier Chia-Hao Shih Margaret Whedon lauren M.Williams Matthew E.davis david A.Henning Se Yun Park Susan d.Calkins jennifer l.Etnier department of Kinesiology University of North Carolina at Greensboro department of Human development and Family Studies University of North Carolina at Greensboro

Background： Attention deficit hyperactivity disorder （AdHd） is a common childhood disorder that affects approximately 11% of children in the United States. Research supports that a single session of exercise benefits cognitive performance by children, and a limited number of studies have demonstrated that these effects can also be realized by children with AdHd. The purpose of this study was to examine the effect of acute exercise on cognitive performance by children with and without AdHd. Methods： Children with and without AdHd were asked to perform cognitive tasks on 2 days following treatment conditions that were assigned in a random, counterbalanced order. The treatment conditions consisted of a 30-min control condition on 1 day and a moderate intensity exercise condition on the other day. Results： Exercise significantly benefited performance on all three conditions of the Stroop Task, but did not significantly affect performance on the Tower of london or the Trail Making Test. Conclusion： children with and without AdHd realize benefits in speed of processing and inhibitory control in response to a session of acute exercise, but do not experience benefits in planning or set shifting.

关键词： Executive function Physical activity Stroop Test Tower of london Test Trail Making Test

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