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Bacte.ial Colonization of the.e.uine.Gut;Comparison of Mare.and Foal Pairs by PCR-DGGe

Advance. in Microbiology 2012年第2期2卷 79-86页

作者： jennifer e. earing Andre. C. Durig Gloria L. Ge.lin Laurie.M. Lawre.ce.Michae. D. Flythe Agricultural Re.e.rch Se.vice Forage-Animal Production Research Unit USDA Lexington USA De.artme.t of Animal and Food Scie.ce. University of Kentucky Lexington USA De.artme.t of Animal and Food Scie.ce. University of Kentucky Lexington USA Agricultural Re.e.rch Se.vice Forage-Animal Production Research Unit USDA Lexington USA

Horse., like.all animals, are.born without the.symbiotic microbe. that occupy the.gastrointe.tinal tracts of mature.animals. As grazing animals, horse. re.y on the.e.microbe. to fully utilize.the.grasse. and othe. ce.lulosic fe.ds that the. consume. Thus, colonization of the.foal's gastrointe.tinal tract must occur be.we.n birth and we.ning. The.fe.e. of nine.mare.and foal pairs we.e.sample. from the.day of parturition until 12 we.ks of age. and the.sample. we.e.analyze. by polyme.ase.chain re.ction amplification of the.bacte.ial 16S rRNA ge.e.and de.aturing gradie.t ge. e.e.trophore.is (PCR-DGGe.. The.ge.s from fe.e. of day (d) 0 foals had no or ve.y fe. ( x = 3, n = 6) bands, which indicate. that spe.ie. richne.s was low. The.numbe. of bands incre.se. during the.first 4 days of life. and by d 14 the.foals and mare. had similar numbe.s of bands ( x = 28, n = 23). Some.bands we.e.pre.e.t in young foals, but not in mare. or in foals on d 42 or d 84, which indicate. succe.sion of bacte.ial spe.ie.. Whe. the.PCR-DGGe.profile. we.e.compare. with Dice.s algorithm, all mare.foal pairwise.similaritie. on d 14 and late. we.e.as gre.t as the.pairwise.similaritie. be.we.n mare.. The.e.re.ults are.consiste.t with the.ide. that foals are.born with a ste.ile.gut, colonization proce.ds rapidly, and a mature.microbial community is pre.e.t in the.first fe. we.ks of life.

关键词： Colonization De.e.opme.t Microbial e.ology Ne.nate.

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A rat mode. of multicompartme.tal traumatic injury and he.orrhagic shock induce. bone.marrow dysfunction and profound ane.ia

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Animal Mode.s and e.pe.ime.tal Me.icine 2024年第3期7卷 367-376页

作者： Laure. S.Ke.ly jennifer A.Munle. e.ick e.Pons Kole.kode.B.Kannan e.izabe.h M.Whitle. Le.itia e.Bible.Philip A.e.ron Alicia M.Mohr De.artme.t of Surge.y and Se.sis and Critical Illne.s Re.e.rch Ce.te. University of Florida College of MedicineGainesvilleFloridaUSA Pathoge.e.is LLCGainesvilleFloridaUSA

Background:Se.e.e.trauma is associate. with syste.ic inflammation and organ *** rode.t trauma mode.s are.the.mainstay of postinjury re.e.rch but have.be.n criticize. for not fully re.licating se.e.e.human *** aim of this study was to cre.te.a rat mode. of multicompartme.tal injury which re.re.te. profound traumatic ***:Male.Sprague.Dawle. rats we.e.subje.te. to unilate.al lung contusion and he.orrhagic shock(LCHS),multicompartme.tal polytrauma(PT)(unilate.al lung contusion,he.orrhagic shock,ce.e.tomy,bife.oral pse.dofracture.,or na?ve.***,plasma toll-l ike.re.e.tor 4(TLR4),he.oglobin,sple.n to body we.ght ratio,bone.marrow(BM)e.ythroid proge.itor(CFU-Ge.M,BFU-e.and CFU-e.growth,plasma granulocyte.colony-stimulating factor(G-CSF)and right lung histologic injury we.e.asse.se. on day 7,with significance.de.ine. as p value.e.ults:Polytrauma re.ulte. in marke.ly more.profound inhibition of we.ght gain compare. to LCHS(p=0.0002)along with e.e.ate. plasma TLR4(pe. he.oglobin(pe.large. sple.n to body we.ght ratios(p=0.004).Both LCHS and PT de.onstrate. suppre.sion of CFU-e.and BFU-e.growth compare. to naive.pe.e.ate. in PT compare. to both na?ve.and LCHS(pe.onstrate. significant histologic right lung injury with poor alve.lar wall inte.rity and inte.stitial ***:Multicompartme.tal injury as de.cribe. he.e.e.tablishe. a re.roducible.mode. of multicompartme.tal injury with worse.e. ane.ia,sple.ic tissue.e.large.e.t,we.ght loss,and incre.se. inflammatory activity compare. to a le.s se.e.e.*** may se.ve.as a more.e.fe.tive.mode. to re.re.te.profound traumatic injury to re.licate.the.human inflammatory re.ponse.postinjury.

关键词： ane.ia i nflammation polytrauma pse.dofracture shock

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Diagnostic accuracy of e.OS score.in pre.icting DNR labe.ing afte. ICU admission:A prospe.tive.obse.vational study(e.OS-DNR)

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Journal of Inte.sive.Me.icine 2024年第2期4卷 216-221页

作者： Omar e.Ramadan Ahme. F.Mady Mohamme. A.Al-Odat Ahme. N.Balshi Ahme. W.Ale.re.y Taisy J.Ste.he. She.na R.Diolaso jennifer Q.Gano Wale.d Th.Ale.re.y De.artme.t of Critical Care King Saud Medical CityRiyadhSaudi Arabia Ane.the.ia De.artme.t Faculty of MedicineAin Shams UniversityCairoEgypt Ane.the.ia De.artme.t Faculty of MedicineTanta UniversityTantaEgypt Faculty of Me.icine Alexandria UniversityAlexandriaEgypt Nursing De.artme.t King Saud Medical CityRiyadhSaudi Arabia

Background Re.uscitation can some.ime. be.futile.and making a do-not-re.uscitate.DNR)de.ision is in the.be.t inte.e.t of the.*** e.e.tronic poor outcome.scre.ning(e.OS)score.was de.e.ope. to pre.ict 6-month poor outcome. of critically ill *** e.plore. the.diagnostic accuracy of the.e.OS score.in pre.icting DNR de.isions in the.inte.sive.care.unit(ICU).Me.hods This study was conducte. at the.ICU of a te.tiary re.e.ral hospital in Saudi Arabia be.we.n March and May ***,we.calculate. e.OS score. for all e.igible.conse.utive.admissions afte. 48 h in the.ICU and re.orde. the.DNR *** ability of the.score.to pre.ict DNR was e.plore. using logistic ***'s ide.l cut-off value.was calculate. using the.De.ong me.hod,and diffe.e.t diagnostic accuracy me.sure. we.e.ge.e.ate. with corre.ponding 95%confide.ce.inte.vals(CIs).Re.ults We.e.rolle. 857 patie.ts,125 re.e.ve. a DNR orde. and 732 did *** ave.age.e.OS score.of DNR and non-DNR patie.ts was 28.2±10.7 and 15.2±9.7,*** score.as a pre.ictor of DNR orde.,had an are. unde. re.e.ve. ope.ator characte.istic(AUROC)curve.of 81.8%(95%CI:79.0 to 84.3,Pe.'s ide.l cut-off value.17 was associate. with a se.sitivity of 87.2(95%CI:80.0 to 92.5,Pe.ificity of 63.9(95%CI:60.3 to 67.4,Pe.pre.ictive.value.of 29.2(95%CI:24.6 to 33.8,Pe.ative.pre.ictive.value.of 96.7(95%CI:95.1 to 98.3,Pe.e.OS score.pe.forme. we.l as a diagnostic te.t for patie.ts who will be.labe.e. as DNR during the.r ICU stay.A cut-off score.17 may he.p guide.clinical de.isions to withhold or comme.ce.re.uscitative.me.sure..

关键词： Do-not-re.uscitate.DNR) Re.uscitation Inte.sive.care.unit(ICU) Diagnostic accuracy Se.sitivity Spe.ificity

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e.ucidating re.ulatory proce.se. of inte.se.physical activity by multi-omics analysis

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Military Me.ical Re.e.rch 2024年第4期11卷 479-499页

作者： e.ne.to S.Nakayasu Marina A.Gritse.ko Young-Mo Kim jennifer e.Kyle.Ke.ly G.Stratton Carrie.D.Nicora Nathalie.Munoz Kathle.n M.Navarro Danie. Claborne.Yuqian Gao Karl K.We.tz Vane.sa L.Paurus Ke.t J.Bloodsworth Ke.se. A.Alle. Lisa M.Brame. Fe.nando Monte. Kathle.n A.Clark Grant Tie.je.Justin Te.guarde. Kristin e.Burnum-Johnson Biological Scie.ce. Division Pacific Northwest National LaboratoryRichlandWA 99352USA e.vironme.tal and Mole.ular Scie.ce. Division Pacific Northwest National LaboratoryRichlandWA 99352USA Ce.te.s for Dise.se.Control and Pre.e.tion National Institute for Occupational Safety and HealthWestern States DivisionDenverCO 80204USA Computational Analytics Division Pacific Northwest National LaboratoryRichlandWA 99352USA National Se.urity Dire.torate Pacific Northwest National LaboratoryRichlandWA 99352USA Los Ange.e. County Fire.De.artme.t Los AngelesCA 90063USA Ce.te.s for Dise.se.Control and Pre.e.tion National Institute for Occupational Safety and HealthRespiratory Health DivisionMorgantownWV 26505USA e.vironme.tal and Mole.ular Toxicology Oregon State UniversityCorvallisOR 97331USA

Background:Physiological and bioche.ical proce.se. across tissue. of the.body are.re.ulate. in re.ponse.to the.high de.ands of inte.se.physical activity in se.e.al occupations,such as fire.ighting,law e.force.e.t,military,and sports.A be.te. unde.standing of such proce.se. can ultimate.y he.p improve.human pe.formance.and pre.e.t illne.se. in the.work ***:To study re.ulatory proce.se. in inte.se.physical activity simulating re.l-life.conditions,we.pe.forme. a multi-omics analysis of 3 biofluids(blood plasma,urine.and saliva)colle.te. from 11 wildland fire.ighte.s be.ore.and afte. a 45 min,inte.se.e.e.cise.*** profile. ***-e.e.cise.we.e.compare. by Stude.t’s t-te.t followe. by pathway analysis and comparison be.we.n the.diffe.e.t omics ***:Our multi-omics analysis ide.tifie. and quantifie. 3835 prote.ns,730 lipids and 182 me.abolite. combining the.3 diffe.e.t type. of *** blood plasma analysis re.e.le. signature. of tissue.damage.and acute.re.air re.ponse.accompanie. by e.hance. carbon me.abolism to me.t e.e.gy *** urine.analysis showe. a strong,concomitant re.ulation of 6 out of 8 ide.tifie. prote.ns from the.re.in-angiote.sin syste. supporting incre.se. e.cre.ion of catabolite.,re.bsorption of nutrie.ts and mainte.ance.of fluid *** saliva,we.obse.ve. a de.re.se.in 3 pro-inflammatory cytokine. and an incre.se.in 8 antimicrobial pe.tide..A syste.atic lite.ature.re.ie. ide.tifie. 6 pape.s that support an alte.e. susce.tibility to re.piratory ***:This study shows simultane.us re.ulatory signature. in biofluids indicative.of home.static mainte.ance.during inte.se.physical activity with possible.e.fe.ts on incre.se. infe.tion susce.tibility,sugge.ting that caution against re.piratory dise.se. could be.e.it worke.s on highly physical de.anding jobs.

关键词： Multi-omics analysis Inte.se.e.e.cise Human pe.formance Biofluids Me.abolism Immunity

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Gate.controlle. supe.curre.t e.fe.t in dry-e.che. Daye. bridge. of non-ce.trosymme.ric niobium rhe.ium

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Nano Re.e.rch 2024年第7期17卷 6575-6581页

作者： jennifer Koch Carla Cirillo Se.astiano Battisti Le.n Ruf Zahra Makhdoumi Kakhaki Ale.sandro Paghi Arme. Gulian Se.afim Te.nowijoyo Giorgio De.Simoni France.co Giazotto Carmine.Attanasio e.ke.Sche.r Ange.o Di Be.nardo De.artme.t of Physics University of KonstanzUniversitätsstraße 1078464 KonstanzGermany CNR-Spin c/o Universitàdegli Studi di Salernovia Giovanni Paolo II 13284084 Fisciano(SA)Italy Ne.T Istituto Nanoscienze-CNR and Scuola Normale SuperiorePiazza San Silvestro 1256127 PisaItaly Dipartime.to di Fisica“e.R.Caianie.lo” Universitàdegli Studi di SalernoVia Giovanni Paolo II 13284084 Fisciano(SA)Italy Advance. Physics Laboratory Institute for Quantum StudiesChapman UniversityBurtonsvilleMD 20866USA

The.application of a gate.voltage.to control the.supe.conducting curre.t flowing through a nanoscale.supe.conducting constriction,name. as gate.controlle. supe.curre.t(GCS),has raise. gre.t inte.e.t for fundame.tal and te.hnological *** gain a de.pe. unde.standing of this e.fe.t and de.e.op supe.conducting te.hnologie. base. on it,the.mate.ial and physical parame.e.s crucial for the.GCS e.fe.t must be.***-down fabrication protocols should also be.optimize. to incre.se.de.ice.scalability,although studie. sugge.t that top-down fabricate. de.ice. are.more.re.ilie.t to show a ***,we.inve.tigate.gate. supe.conducting nanobridge. made.with a top-down fabrication proce.s from thin films of the.nonce.trosymme.ric supe.conductor niobium rhe.ium with varying ratios of the.constitue.ts(NbRe..Unlike.othe. de.ice. pre.iously re.orte. and made.with a top-down approach,our NbRe.de.ice. syste.atically e.hibit a GCS e.fe.t whe. the. we.e.fabricate. from NbRe.thin films with small grain size.and e.che. in spe.ific *** obse.vations pave.the.way for the.re.lization of top-down-made.GCS de.ice. with high *** re.ults also imply that physical parame.e.s like.structural disorde. and surface.physical prope.tie. of the.nanobridge.,which can be.in turn modifie. by the.fabrication proce.s,are.crucial for a GCS obse.vation,providing the.e.ore.also important insights into the.physics unde.lying the.GCS e.fe.t.

关键词： supe.conductivity non-ce.trosymme.ric supe.conductors supe.conducting de.ice. gate. de.ice. gate.controlle. supe.curre.t top-down fabrication

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Carbon Se.ue.tration through CO2 Foam-e.hance. Oil Re.ove.y： A Gre.n Che.istry Pe.spe.tive

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e.gine.ring 2018年第3期4卷 336-342页

作者： jennifer A. Clark e.ik e. Santiso De.artme.t of Che.ical and Biomole.ular e.gine.ring North Carolina State University Raleigh NC 27695 USA

e.hance. oil re.ove.y （e.R） via carbon dioxide.（CO2） flooding has re.e.ve. a conside.able.amount of atte.tion as an e.onomically fe.sible.me.hod for carbon se.ue.tration, with many re.e.t studie. focus- ing on de.e.oping e.hance. CO2 foaming additive., Howe.e., the.pote.tial long-te.m e.vironme.tal e.fe.ts of the.e.additive. in the.e.e.t of le.kage.are.poorly unde.stood and, give. the.amount of additive. inje.te. in a typical CO2 e.R ope.ation, could he.far-re.ching, This pape. pre.e.ts a summary of re.e.t de.e.opme.ts in surfactant and surfactant/nanoparticle.hase. CO2 foaming syste.s, with an e.phasis on the.possible.e.vironme.tal impacts of CO2 foam le.kage. Most of the.surfactants studie. are.unlike.y to de.rade.unde. re.e.voir conditions, and the.r re.e.se.can cause.major ne.ative.impacts on wildlife. With re.e.t advance. in the.use.of additive. （e.g,, nonionic surfactants, nanoparticle., and othe. che.icals） the.use.of harsh anionic surfactants may no longe. he.warrante., This pape. discusse. re.e.t advance. in producing foaming syste.s, and highlights possible.strate.ie. to de.e.op e.vironme.tally frie.dly CO2 e.R me.hods,

关键词： Surfactants Nanoparticle. Carbon se.ue.tration e.hance. oil re.ove.y

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He.lo, goodbye. and e.e.ything in be.we.n

Hello, goodbye, and everything in between

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作者： Smith jennifer e.

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Cdc42 re.ulate. the.ce.lular localization of Cdc42e.1 in controlling ne.ral cre.t ce.l migration

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Journal of Mole.ular Ce.l Biology 2018年第5期10卷 376-387页

作者： Shlomi Cohe. Danie. T. Kovari We.bin We. Re.e.ca Ke.te.jennifer e. Curtis Shuyi Nie School of Biological Scie.ce. Georgia institute of Technology Atlanta GA 30332 USA School of Physics Georgia Institute of Technology Atlanta GA 30332 USA Pe.it Institute.for Bioe.gine.ring and Bioscie.ce Georgia Institute of Technology Atlanta GA 30332 USA De.artme.t of Physics Emory University Atlanta GA 30322 USA Wallace.H. Coulte. De.artme.t of Biome.ical e.gine.ring Georgia institute of Technology Atlanta GA 30332 USA

The.me.be. of Rho family of small GTPase. Cdc42 plays important and conse.ve. role. in ce.l polarity and motility. The.Cdc42e. family prote.ns have.be.n ide.tifie. to bind to Cdc42, ye. how the. inte.act with Cdc42 to re.ulate.ce.l migration re.ains to be.e.ucidate.. In this study, we.focus on Cdc42e.l, which is e.pre.se. pre.ominantly in the.highly migratory ne.ral cre.t ce.ls in frog e.bryos. Through morpholino-me.iate. knockdown, we.show that Cdc42e.l is re.uire. for the.migration of cranial ne.ral cre.t ce.ls. Loss of Cdc42e.l le.ds to rounde. ce.l shape. and the.formation of me.brane.ble.s, consiste.t with the.obse.ve. disruption in actin organization and focal adhe.ion alignme.t. As a re.ult, Cdc42e.1 is critical for ne.ral cre.t ce.ls to apply traction force. at the.corre.t place.to migrate.e.ficie.tly. We.furthe. show that Cdc42e.1 is localize. to two are.s in ne.ral cre.t ce.ts： in me.brane.protrusions toge.he. with Cdc42 and in pe.inucie.r patche. whe.e.Cdc42 is abse.t. Cdc42 dire.tly inte.acts with Cdc42e.l （through the.CRIB domain） and change. in Cdc42 le.e. shift the.distribution of Cdc42e.l be.we.n the.e.two subce.lular locations, controlling the.formation of me.brane.protrusions and dire.tionality of migration as a conse.ue.ce. The.e.re.ults sugge.t that Cdc42e.1 e.aborate. Cdc42 activity in ne.ral cre.t ce.ls to promote.the.r e.ficie.t migration.

关键词： actin cytoske.e.on ne.ral cre.t Rho GTPase. Cdc42 e.fe.tor prote.n 1 ce.l migration

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Virus-Induce. Ge.e.Sile.cing in the.Culinary Ginge. （Zingibe. officinale.： An e.fe.tive.Me.hanism for Down-Re.ulating Ge.e.e.pre.sion in Tropical Monocots

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Mole.ular Plant 2009年第5期2卷 1084-1094页

作者： Tanya Re.ne. jennifer Bragg He.the. e. Driscoll Juliana Cho Andre. O. Jackson Che.se. D. Spe.ht De.artme.t of Plant and Microbial Biology University of California Berkeley CA 94720 USA Pre.e.t addre.s: Ge.ornics and Ge.e.Discove.y USDA ARS PWA WRRC-GGD 800 Buchanan Street Albany CA 94710 USA

Virus-induce. ge.e.sile.cing （VIGS） has be.n shown to be.e.fe.tive.for transie.t knockdown of ge.e.e.pre.sion in plants to analyze.the.e.fe.ts of spe.ific ge.e. in de.e.opme.t and stre.s-re.ate. re.ponse.. VlGS is we.l e.tablishe. for studie. of mode. syste.s and crops within the.Solanace.e. Brassicace.e. Le.uminace.e. and Poace.e. but only re.e.tly has be.n applie. to plants re.iding outside.the.e.familie.. He.e. we.have.de.onstrate. that barle. stripe.mosaic virus （BSMV） can infe.t two spe.ie. within the.Zingibe.ace.e. and that BSMV-VIGS can be.applie. to spe.ifically down-re.ulate.phytoe.e.de.aturase.in the.culinary ginge. Zingibe. officinale. The.e.re.ults sugge.t that e.te.sion of BSMV-VlGS to monocots othe. than ce.e.ls has the.pote.tial for dire.te. ge.e.ic analyse. of many important te.pe.ate.and tropical crop spe.ie..

关键词： Barle. stripe.mosaic virus virus-induce. ge.e.sile.cing VIGS Zingibe. officinale Monocot.

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Advance.e.ts and challe.ge. in tre.ting advance. gastric cance. in the.We.t

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World Journal of Gastrointe.tinal Oncology 2019年第9期11卷 652-664页

作者： jennifer L Le.ting Travis e.Grotz Division of He.atobiliary and Pancre.s Surge.y Mayo Clinic

Gastric cance. is a le.ding cause.of cance. incide.ce.and de.th *** with advance. gastric cance. be.e.it from a multi-modality tre.tme.t *** oncologic re.e.tion with ne.ative.margins and comple.e.lymphade.e.tomy plays a crucial role.in long-te.m survival for patie.ts with re.e.table.*** utilization of minimally invasive.te.hnique. for gastric cance. has be.n slowly incre.sing and is proving to be.both te.hnically and oncologically *** che.othe.apy is the.curre.t standard of care.for advance. gastric cance..A varie.y of che.othe.apy re.ime.s have.be.n use. with the.combination of doce.axe.,oxaliplatin,5-fluorouracil,and le.covorin be.ng the.curre.t re.omme.dation give. its supe.ior ability to induce.a comple.e.pathologic re.ponse.and prolong *** use.of radiation has be.n more.controve.sial with its optimal place.in the.tre.tme.t se.ue.ce.be.ng *** are.curre.t ongoing studie. asse.sing the.impact of radiation as an adjunct or in place.of *** tre.tme.ts(e.g.,trastuzumab for human e.ide.mal growth factor re.e.tor 2 positive.tumors and pe.brolizumab for programme. de.th-ligand 1 positive.tumors)are.showing promise.and are.part of a continue. e.phasis on individualize. *** che.othe.apy may also play a role.in pre.e.ting pe.itone.l re.urre.ce. for patie.ts with high risk *** tre.tme.t of patie.ts with advance. gastric cance. in the.We.t continue. to advance.and improve.with a be.te. unde.standing of optimal tre.tme.t se.ue.ce. and the.utilization of pe.sonalize. tre.tme.t re.ime.s.

关键词： Gastric cance. D2 lymphade.e.tomy Minimally invasive surge.y Ne.adjuvant che.othe.apy Che.oradiation Targe.e. tre.tme.ts

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