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Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation

Nano-Micro Letters 2020年第12期12卷 404-420页

作者： Laurens Raes Stephan Stremersch Juan CFraire Toon Brans Glenn Goetgeluk Stijn De Munter Lien van hoecke Rein Verbeke jelter van hoeck Ranhua Xiong Xavier Saelens Bart vandekerckhove Stefaan De Smedt Koen Raemdonck Kevin Braeckmans Laboratory of General Biochemistry&Physical Pharmacy Ghent University9000 GhentBelgium Cancer Research Institute Ghent(CRIG) 9000 GhentBelgium Department of Diagnostic Sciences Ghent University9000 GhentBelgium VIB-UGent Center for Medical Biotechnology 9052 GhentBelgium Department of Biomedical Molecular Biology Ghent University9000 GhentBelgium Department of Biochemistry and Microbiology Ghent University9000 GhentBelgium

Efficient and safe cell engineering by transfection of nucleic acids remains one of the long-standing hurdles for fundamental biomedical research and many new therapeutic applications,such as CAR T cell-based *** has recently gained increasing attention as a more safe and versatile alternative tool over viral-or DNA transposon-based approaches for the generation of adoptive T ***,limitations associated with existing nonviral mRNA delivery approaches hamper progress on genetic engineering of these hard-to-transfect immune *** this study,we demonstrate that gold nanoparticle-mediated vapor nanobubble(VNB)photoporation is a promising upcoming physical transfection method capable of delivering mRNA in both adherent and suspension *** transfection experiments on HeLa cells showed the importance of transfection buffer and cargo concentration,while the technology was furthermore shown to be effective for mRNA delivery in Jurkat T cells with transfection efficiencies up to 45%.Importantly,compared to electroporation,which is the reference technology for nonviral transfection of T cells,a fivefold increase in the number of transfected viable Jurkat T cells was ***,our results point toward the use of VNB photoporation as a more gentle and efficient technology for intracellular mRNA delivery in adherent and suspension cells,with promising potential for the future engineering of cells in therapeutic and fundamental research applications.

关键词： Transfection mRNA Photoporation Optoporation Gold nanoparticles Vapor nanobubbles

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Chromium propionate improves performance and carcass traits in broilers

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Animal Nutrition 2020年第4期6卷 480-487页

作者： Veerle van hoeck Mahendra Sonawane Antonio L.Gonzalez Sanchez Iris van Dosselaer Chris Buyens Dany Morisset Kemin Europa N.V. Animal Nutrition and Health EMENAToekomstlaan 422200HerentalsBelgium

There is evidence to suggest that poultry may have a dietary requirement for metabolically available chromium(Cr)that exceeds the amount provided through wheat soybean meal *** objective of the present study was to investigate the effects of dietary supplemental organic Cr from Cr propionate at different dose levels(control=0μg/kg,T1=200μg/kg,T2=400μg/kg)on the growth performance,carcass traits,and meat quality of *** gain and feed intake of each treatment were recorded at the start and after 14,28 and 35 d,and feed conversion ratios(FCR)were calculated ***35 d of age,birds were randomly selected and euthanized for carcass *** of the first trial indicate that both Cr propionate treatments increased final body weight(P<0.05),feed efficiency(P<0.05)and body weight gain(P<0.0001).Furthermore,Cr propionate supplementation improved(P<0.0001)all carcass ***,with increased Cr dosage,carcass yield,dressing percentage and breast meat yield increased linearly(P<0.0001).The second study reveals that the feed intake in the control group was significantly higher compared to both Cr propionate supplemented groups(T1&T2).Furthermore,the Cr propionate supplemented T2 group displayed a significantly lower FCR than the control and T1 group(P=0.027).Finally,Cr propionate supplementation increased the dressing percentage compared to control birds(P<0.0001).In the third experiment,Cr propionate supplementation(T1&T2)increased final body weight and decreased FCR compared with the control *** effects were highly significant(P<0.0001)throughout all feeding phases of the *** propionate supplementation also increased(P<0.0001)carcass yield,dressing percentage,breast meat yield,leg and thigh weights compared with the control *** conclusion,growth performance,feed conversion,carcass yield,breast and leg meats of broiler birds can be significantly improved by dietary inclusion of Cr *** pro

关键词： Chromium propionate Broiler Performance Carcass trait

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Evidence of endometrial amino acid metabolism and transport modulation by peri-ovulatory endocrine profiles driving uterine receptivity

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Journal of Animal Science and Biotechnology 2017年第4期8卷 852-865页

作者： Moana Rodrigues Franca Maressa Izabel Santos da Silva Guilherme Pugliesi Veerle van hoeck Mario Binelli Department of Animal Reproduction School of Veterinary Medicine and Animal ScienceUniversity of Sao Paulo225Duque de Caxias Norte Ave.Jd.Elite13635-900 PirassunungaSPBrazil Gamete Research Centre University of AntwerpAntwerpBelgium

Background: In beef cattle, changes in the periovulatory endocrine milieu are associated with fertility and conceptus growth. A large preovulatory follicle(POF) and the resulting elevated concentrations of progesterone(P4) during diestrus positively affect pregnancy rates. Amino acids(AA) are important components of maternally derived secretions that are crucial for embryonic survival before implantation. The hypothesis is that the size of the POF and the concentration of P4 in early diestrus modulate the endometrial abundance of SLC transcripts related to AA transport and metabolism and subsequently impact luminal concentrations of AA. The follicle growth of Nelore cows was manipulated to produce two experimental groups: large POF and CL(LF-LCL group) and small POF and CL(SF-SCL group). On Day 4(D4; Experiment 1) and Day 7(D7; Experiment 2) after Gn RH-induced ovulation(Gn RH treatment = D0), the animals were slaughtered and uterine tissues and uterine washings were collected. q RT-PCR was used to evaluate the expression levels of AA transporters in D4 and D7 endometrial *** concentrations of AA were quantified in D4 and D7 uterine washings by ***: Transcript results show that, on D4, SLC6 A6, SLC7 A4, SLC17 A5, SLC38 A1, SLC38 A7 and SCLY and on D7 SLC1 A4, SLC6 A1, SLC6 A14, SLC7 A4, SLC7 A7, SLC7 A8, SLC17 A5, SLC38 A1, SLC38 A7, SLC43 A2 and DDO were more abundant in the endometria of cows from the LF-LCL group(P < 0.05). In addition, concentrations of AA in the uterine lumen were influenced by the endocrine profiles of the mother. In this context, D4 uterine washings revealed that greater concentrations of taurine, alanine and α-aminobutyric acid were present in SF-SCL(P < 0.05).In contrast, lower concentrations of valine and cystathionine were quantified on D7 uterine washings from SF-SCL cows(P < 0.05).Conclusion: The present study revealed an association between the abundance of transcripts related to AA transport and metabolism in the endo

关键词： Amino acids Beef cattle Sex steroids Uterus

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