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世界核心医学期刊文摘（胃肠病学分册） 2005年第1期1卷 15-16页

作者： jelski w. Zalew.ki B. Chrostek L. Szmitkow.ki M. 杨瑗

Chronic ethanol consumption is associated w.th an increased risk for cancer of the colorectum. The highly toxic and carcinogenic compound is acetaldehyde, the product of ethanol metabolism. Ethanol is metabolized to acetaldehyde by alcohol dehydrogenase (ADH) in colorectal mucosa and bacteria. The enzyme responsible for oxidation of acetaldehyde is aldehyde dehydrogenase. The aim of this study w.s to compare ADH isoenzymes and ALDH activity in colorectal cancer w.th the activity in normal colonic mucosa. Total ADH activity w.s measured by a photometric method w.th p-nitrosodimethylaniline(NDMA)as substrate, and ALDH activity by a fluorometric method w.th 6-methoxy-2-naphthaldehyde as a substrate. For measurement of the activity of class I and II isoenzymes w. employed fluorometric methods,w.th class-specific fluorogenic substrates. The activity of class III ADH w.s measured by the photometric method w.th n-octanol as substrate, and class IV w.th m-nitrobenzaldehyde as substrate. Samples w.re taken surgically during routine operations of colorectal carcinomas from 32 patients. The activities of total ADH and, the most important in colon mucosa, class I ADH w.re significantly higher in cancer than in healthy tissues. The other tested classes of ADH had a tendency to higher-level activity in cancer cells than in healthy mucosa. ALDH activity w.s not significantly low.r in the cancer cells. The activities of all tested enzymes and isoenzymes w.re not significantly higher in drinkers than in nondrinkers both in colorectal cancer and in normal *** differences in activities of total ADH and class I isoenzyme betw.en cancer tissues and normal colon mucosa might be a factor for metabolic changes and disturbances in low.mature cancer cells and, additionally, might be a reason for the higher level of acetaldehyde, w.ich can intensify carcinogenesis.

关键词：结肠直肠癌乙醛脱氢酶二甲基苯低分化癌细胞正常结肠黏膜正常黏膜同工酶荧光测定法光度测定法切除物

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