检索结果-南通市图书馆

Comparative Assessment of Growth and Gene Regulation between 4 Serotypes of <i>Streptococcus pneumoniae</i>in Broth and Cell Culture

引用

Advances in Microbiology 2018年第10期8卷 763-777页

作者： Ben Seabolt jeff brady Janice Speshock Department of Biological Sciences Tarleton State University Stephenville TX USA Texas A&M AgriLife Research and Extension Center Stephenville TX USA

Streptococcus pneumoniae is a medically important pathogen capable of causing human infections of pneumonia, bacteremia, otitis media, and meningitis. Although there are vaccinations available, infections with S. pneumoniae still remains a global problem. S. pneumoniae is a highly adaptable bacterial species with numerous serotypes based on capsular polysaccharides. The different serotypes vary in their ability to colonize and causing pathology. Here we compared the regulation of five different virulence factors from four common serotypes of S. pneumoniae that vary in their carriage, morbidity, and mortality rates in the human population using two different in vitro methods, broth and cell culture. We determine that there is variation of virulence factor gene regulation within a serotype using two different culture methods, and variation between the serotypes in the same culture condition. The regulation of genes appeared to have a correlation with the ability of the various serotypes to grow in broth culture, adhere to cultured lung cells, and invade the cultured lung cells, as serotypes that shared similar regulation of virulence factors tended to behave similarly in culture. Many studies with S. pneumoniae rely on the use of one selected serotype, but since there is a wide variation in the growth and regulatory mechanisms of these bacteria. As demonstrated here, future studies should utilize more strains in models before concluding mechanisms of pathobiology.

关键词： Pneumococcus Serotypes Gene Expression Regulation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A New Technique for Use in Culturing Prokaryotes Comprising the Mouse Intestinal Microbiome

引用

Advances in Microbiology 2023年第3期13卷 119-147页

作者： Everest Uriel Castaneda Kelly Carroll Janice Speshock jeff brady Department of Biological Sciences Tarleton State University Stephenville Texas USA Texas A&M AgriLife Research and Extension Center Stephenville Texas USA

The microbiome has a profound impact on host fitness. pH, oxygen, nutrients, or other factors such as food or pharmaceuticals, subject the microbiome to variations in the gastrointestinal tract. This variation is a cause for concern given dysbiosis of the microbiome is correlated with various disease states. Currently, much research relies on model organisms to study microbial communities since intact microbiomes are challenging to utilize. The objective of this study is to culture an explanted colon microbiome of 4 Balb/c mice to develop an in vitro tool for future microbiome studies. We cultured homogenates of the distal colons of 4 mice in trans-well culture dishes. These dishes were incubated for 24 hours in two different oxygen concentration levels and the pH was compared before and after incubation of the cultures. To analyze the integrity of the microbiome, we utilized massively paralleled DNA sequencing with 16S metagenomics to characterize fecal and colon samples to speculate whether future studies may utilize feces in constructing an in vitro microbial community to spare animal lives. We found that pH and familial relationships had a profound impact on community structure while oxygen did not have a significant influence. The feces and the colon were similar in community profiles, which lends credence to utilizing feces in future studies. The gut microbiome is of great interest and great importance for studies in a variety of different diseases. Many laboratories do not have access to germ-free mice, which is one optimal way to study mammalian microbiomes, but this technique allowed for the in vitro culturing of a majority of the prokaryotes isolated from the colons of mice. This may allow an alternative to study the interactions of this very diverse population of microorganisms without the need for germ-free conditions.

关键词： Microbiome ex vivo Massively Paralleled Sequencing pH Oxygen

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Impact of Manure Storage Time and Temperature on Microbial Composition and Stable Fly (Diptera: Muscidae) Development

引用

Advances in Microbiology 2019年第3期9卷 248-265页

作者： Janice L. Speshock jeff A. brady Jessica Eastman Travis Roach Samantha Hays David Kattes Department of Biology Tarleton State University Stephenville USA Department of Wildlife Sustainability & Ecosystem Sciences Tarleton State University Stephenville USA Texas A&M AgriLife Research & Extension Center Stephenville USA Department of Agriculture & Environmental Science Tarleton State University Stephenville USA

Samples are often frozen for preservation until needed for use. It has been a common practice to store fresh dairy manure in the freezer until needed for fly development studies. However, conflicting data have suggested that freezer temperature and duration of manure may impact fly development studies, and it is likely due to the change in microbial comminutes due to the freezer conditions. In this study manure storage conditions were assessed to ascertain how temperatures impact stable fly, Stomoxys calcitrans L., survival to pupation and determine which bacterial populations impacted fly development using massively-parallel sequencing and 16S metagenomic analysis. Stable fly survival to pupation was greater in manure that was stored warm (27?C) or frozen (-20?C or -80?C) for 24 days as compared to fresh manure samples. Refrigeration (4?C) of the manure for 24 days did not affect fly development and slightly decreased the pupal weights. Over 80 bacterial families were detected by sequencing allowing for a more thorough assessment of changes in bacterial populations. Only minor shifts were observed in bacterial family composition in the manure when refrigerated or frozen for 24 days, but significant population changes were observed when the manure was incubated for 24 days at 27?C. Since it is the temperature and incubation time that yielded the greatest pupation rate, it is hypothesized that the manure microbial community impacts the growth and development of stable flies. This study has determined suggested freezer conditions for the best storage of manure samples to maintain bacterial diversity and retain the closest bacterial populations to freshly collected manure. Although untouched, aged (20 days) manure is best to use to assess fly development, it is not always feasible in laboratory experimentations. This study demonstrates the importance of preservation techniques on manure samples, which could also confer to storage of other biological specimens that cont

关键词： Stomoxys calcitrans L. Manure Microbiota 16S Metagenomics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A New Technique for Use in Culturing Prokaryotes Comprising the Mouse Intestinal Microbiome

引用

Advances in Microbiology（微生物学） 2023年第3期

作者： Everest Uriel Castaneda Kelly Carroll Janice Speshock jeff brady

来源：博看期刊评论

在线全文

博看期刊

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：