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Molecular Characterization and Prevalence of Trypanosoma Species in Cattle from a Northern Livestock Area in Cote d’Ivoire

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Open Journal of Veterinary Medicine 2014年第12期4卷 314-321页

作者： Isidore Kpandji Kouadio Didier Sokouri Mathurin Koffi Ibrahim Konate Bernadin Ahouty Alain Koffi simon Pierre N’Guetta Laboratoire de Genetique UFR BiosciencesUniversite Felix Houphouet BoignyAbidjanCote d’Ivoire Laboratoire des Interaction Hote-Microorganisme-Environnement et Evolution Universite Jean Lorougnon GuedeDaloaCote d’Ivoire Centre Suisse de Recherches Scientifiques en Cote d’Ivoire AbidjanCote d’Ivoire Institut Pierre Richet Unite de Recherche TrypanosomosesBouakeCote d’Ivoire

Background: African animal trypanosomiasis (AAT) is caused mainly by Trypanosoma congolense, T. vivax, and T. brucei brucei and is the major constraint for livestock productivity in Sub-Saharan African countries. Information about animal trypanosomiasis status in Ivory Coast is missing, especially regarding molecular epidemiology. Therefore, this study intended to apply molecular tools to identify and characterize trypanosomes in Ivory Coast for sustainable control. Methods: 363 cattle blood samples were collected from Ferkessedougou Region in northern Ivory Coast in 2012. Buffy coat technique (BCT) and species-specific PCR assays were used to detect trypanosome species. Results: Out of 363 cattle examined with BCT, 33 were found positive with all trypanosomes species accounting for an average of 9.09% prevalence whereas polymerase chain reaction (PCR) using species-specific primers showed that 81 out of 363 cattle were infected with trypanosomes with an overall prevalence of 22.31%. Trypanosoma congolense savanah type, T. Vivax and T. brucei sl. accounted for 28.39%, 49.38% and 23.45% of the infection rate respectively. No infection with T. congo forest?type was detected. T. vivax infection was the most prevalence in the area investigated compared to the two other trypanosome species. Mixed infections with different trypanosomes species were detected accounting for 7.32% of prevalence. Regarding sexrelated prevalence, male cattles were slightly more infected than female but the difference was not significant. Conclusion: Our results showed that there was a high prevalence of AAT in livestock in Ferkessedougou Area. There is therefore a need to strengthen control policies and institute measures that help prevent the spread of the parasites for sustainable control of animal trypanosome in this area.

关键词： Animal African Trypanosomiasis Molecular Diagnosis Species-Specific PCR Cote d’Ivoire

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Telehealth in the management of diabetes mellitus in China

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Chinese Medical Journal 2012年第23期125卷 4307-4311页

作者： ZHU Bin LIU Mai-lan XU Shu-qiang Jun Chen Collet jean-Paul Collet Scott A Lear LI Ping Institute of Clinical Medical Sciences China-Japan Friendship Hospital Beijing 100029 China Child and Family Research Institute Vancouver Canada Acupuncture and Tuina College Chengdu University of Traditional Chinese Medicine Chengdu Sichuan 610072 China Infection Prevention and Control Service Provincial Health Service Authority British Columbia Canada UBC Department of Pediatrics Child and Family Research Institute Vancouver Canada Faculty of Health Sciences Simon Fraser University Harbour Centre Campus Vancouver Canada

Telehealth was defined as the use of information, computers and telecommunication technologies to provide health related services when health professionals and patients are separated by a distance This new medical model involves video-conference, transmission of images, e-health portals, remote monitoring of vital signs, and continuing medical education as a whole to help patients to improve health care. It should consist of three parts, including the patient client, the specialist and the connector （platform and intemet）.

关键词： telehealth diabetes mellitus management of diabetes

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释放阳光地带的光伏发电潜力

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电力与能源 2011年第2期32卷 144-148页

作者： Jochen Hauff Marnik Verdonck Harold Derveaux Laurent Dumarest Jose Alberich jean-Charles Malherbe Adel El Gammal Paula Llamas Ga.tan Masson Ernesto Macias Guido Glania simon Rolland 科尼尔咨询公司欧洲光伏产业协会农村电气化联盟

拥有非常丰富的太阳能辐射资源的阳光地带,人口占全球人口总数的75%,电力需求占全球总需求的40%,该地区经济发展受到能源和环境的制约,充分利用丰富的太阳能辐射资源开发光伏发电产业,是可持续发展的重要手段。预测结果表明,范式转型情... 详细信息

拥有非常丰富的太阳能辐射资源的阳光地带,人口占全球人口总数的75%,电力需求占全球总需求的40%,该地区经济发展受到能源和环境的制约,充分利用丰富的太阳能辐射资源开发光伏发电产业,是可持续发展的重要手段。预测结果表明,范式转型情景中,这些国家的光伏装机容量甚至可达1 100 GW,也就是该地域发电总量的12%;2020年阳光地带国家光伏系统的发电成本将比建设天然气和燃油电站更具竞争力;至2030年光伏发电可以与所有以煤和天然气为燃料的中负荷电厂竞争。阳光地带国家发展股份发电目前还面临着许多障碍,这些障碍涉及到政府对燃油价格的补贴、服务于市场的能力以及能源公司的知识有限等,这些障碍有待于在发展过程中不断克服。中国和印度是阳光地带中的大国,两国的光伏制造业对阳光地带的影响很大。新能源被锁定为中国十二五规划的七大战略性新兴产业之一,中国的十二.五规划的重心是重新平衡经济增长模式,包括逐步扩大内需;国内光伏市场一旦打开,光伏在中国的发展将会非常迅速。释放阳光地带光伏潜力要求众多利益相关者共同参与协作,包括政府、银行和金融机构以及各行各业的支持,才能充分释放阳光地带的光伏发电潜力,带动光伏发电产业链的发展,成为社会经济可持续发展的重要组成部分。

关键词：光伏发电阳光地带发展潜力产业竞争力情景分析

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Contribution of Automated Antigen Tests, the LumiraDx Ag Test in the Response during the Second Wave of the COVID-19 Pandemic in Bangui

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Journal of Tuberculosis Research 2023年第4期11卷 173-183页

作者： Clotaire Donatien Rafaï Pierre Somse Wilfrid Sylvain Nambei Ernest Lango-Yaya Marie-Roseline Darnycka Belizaire Ulrich Vickos Narcisse Patrice Komas Oscar Senzongo Luc Salva Heredeibona Ulrich Jeffrey Kotemossoua Rabbi Mermoz Senekian simon Pounguinza Jephté Estimé Kaleb Kandou Christian-Diamant Mossoro-Kpinde Laurent Bélec jean De Dieu Longo Norbert Richard Ngbale Abdoulaye Sepou François-Xavier Mbopi-Keou Gérard Grésenguet Boniface Koffi National Laboratory of Clinical Biology and Public Health Bangui Central African Republic Faculty of Health Sciences University of Bangui Bangui Central African Republic Ministry of Health and Population Bangui Central African Republic World Health Organization Bangui Central African Republic Institut Pasteur de Bangui Bangui Central African Republic Virology Laboratory Georges Pompidou European Hospital Université Paris Cité Paris France Hôpital Communautaire of Bangui Bangui Central African Republic Faculty of Medicine and Biomedical Sciences University of Yaounde I Yaounde Cameroon

Context and objective: The COVID-19 pandemic has become a major public health problem and has mobilized many innovative means of diagnosis. The Central African Republic is not spared. The emergence of variants and their impact require health monitoring despite the obligation of vaccination. The purpose of this campaign was to determine the circulation of pending second-wave variants. Patients and Methods: A second mass screening campaign took place from 02 to 22 July 2021 in the main land and river entry points of Bangui (Exit North-PK12, Exit South-PK9, Port Beach) and at the LNBCSP. Antigenic and RT-PCR tests carried out on nasopharyngeal samples made it possible to select strains which were finally sequenced. Results: Of 2687 participants included in the study, 53 (1.97%) were positive for SARS-CoV-2. Thirteen (1.53%) were male and 40 (2.18%) female. The analyses carried out on the LumiraDx analyzer were positive for 109 samples against 53 on the RT-PCR. The prevalence was higher in the most tested age groups (30 to 50 years) with two clusters identified. B.1.617.2 (Delta) variants were predominant (57%). Conclusion: SARS-CoV-2 continues to circulate. The acquisition of automated antigenic tests (LumiraDx®) with sensitivity and specificity close to those of the reference test (RT-PCR) will allow better mass diagnosis for an optimization of the surveillance of COVID-19 in our countries with limited resources. The predominance of the B.1.617.2 (Delta) variant would suggest a third wave in the Central African Republic.

关键词： COVID-19 Automated Antigen Testing

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Using the Bacterial Protein NupC as a Model for Human Nucleoside Drug Transporters

Using the Bacterial Protein NupC as a Model for Human Nucleo...

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The 4th ISGO International Conference on Structural Genomics

作者： Dawn A.Hadden Shaun K.Loewen jean C.Ingram simon G.Patching Masao Yamashita Matthew J.Conroy David A.Middleton Gary J.Litherland Richard B.Herbert Maurice P.Gallagher Per A.Bullough Peter J.F.Henderson Carol E.Cass James D.Young Stephen A.Baldwin Institute of Membrane and Systems Biology University of Leeds LS2 9JTUK Dept.of Physiology University of Alberta EdmontonAlberta T6G 1Z2Canada Institute of Membrane and Systems Biology University of Leeds LS2 9JTUK Institute of Membrane and Systems Biology University of Leeds LS2 9JTUK Dept.of Molecular Biology and Biotechnology University of Sheffield Sheffield S10 2TNUK Dept.of Molecular Biology and Biotechnology University of Sheffield Sheffield S10 2TNUK Faculty of Life Sciences The University Of Manchester Manchester M60 1QDUK Institute of Membrane and Systems Biology University of Leeds LS2 9JT UK Institute of Cell ＆ Molecular BiologyUniversity of EdinburghEdinburghUK Institute of Membrane and Systems Biology University of Leeds LS2 9JTUK Institute of Cell ＆ Molecular Biology University of Edinburgh Edinburgh UK Dept. of Molecular Biology and Biotechnology University of Sheffield Sheffield S10 2TNUK Institute of Membrane and Systems Biology University of Leeds LS2 9JTUK Dept.of Oncology Cross Cancer Institute 11560 University AvenueEdmonton Alberta T6G 1Z2Canada Dept.of Physiology University of Alberta Edmonton Alberta T6G 1Z2Canada Institute of Membrane and Systems Biology University of Leeds LS2 9JTUK

＜正＞In humans, concentrative nucleoside transporters play important roles in the cellular uptake of chemotherapeutic nucleoside analogues. To provide insights into the mechanism of transport, we have utilised the homologous nucleoside transporter NupC of Escherichia coli as a model system. It proved possible to express this protein at levels up to 20% of the total membrane protein in E. coli and

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Magnetic Facies and Polymetallic Sulphides Deposit in the Mafic and Ultramafic Intrusions of Samapleu (Western Côte d’Ivoire)

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Open Journal of Geology（地质学期刊） 2022年第10期

作者： simon pierre djroh gnamba emmanuel franck gouedji ehui beh jean constantin aka boko célestin sombo christian picard marc-antoine audet bouaké bakayoko

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Long-term results after revisions of failed primary vertical banded gastroplasty

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World Journal of Gastrointestinal Surgery 2016年第3期8卷 238-245页

作者： Martin R van Wezenbeek Frans J F Smulders jean-Paul J G M de Zoete Misha D Luyer Gust van Montfort simon W Nienhuijs Department of Surgery Catharina Hospital

AIM: To compare the results after revision of primary vertical banded gastroplasty(Re-VBG) and conversion to sleeve gastrectomy(cS G) or gastric bypass(cR YGB).METHODS: In this retrospective single-center study, all patients with a failed VBG who underwent revisional surgery were included. Medical charts were reviewed and additional postal questionnaires were sent to update follow-up. Weight loss, postoperative complications and long-term outcome were assessed. RESULTS: A total 152 patients were included in this study, of which 21 underwent Re-VBG, 16 underwent c SG and 115 patients underwent c RYGB. Sixteen patients necessitated a second revisional procedure. No patients were lost-to-follow-up. Two patients deceased during the follow-up period, 23 patients did not return the questionnaire. Main reasons for revision were dysphagia/vomiting, weight regain and insufficient weight loss. Excess weight loss(%EWL) after Re-VBG, cS G and cR YGB was, respectively, 45%, 57% and 72%. Eighteen patients(11.8%) reported postoperative complications and 27% reported long-term complaints. CONCLUSION: In terms of additional weight loss, postoperative complaints and reintervention rate, Rouxen-Y gastric bypass seems feasible as a revision for a failed VBG.

关键词： Vertical banded gastroplasty Conversion Revision Gastric bypass Sleeve gastrectomy Additional weight loss

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The Effect of the COVID-19 Pandemic on Health Service Accessibility for Children under 5 in the Mayo-Tsanaga and Logone & Chari Departments, Far North Cameroon

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Health 2023年第10期15卷 1047-1058页

作者： Ulrich Dama Desire Tchoffo jean Ndibi Abanda François Anicet Onana Akoa Alphonse Tedonge Asobochia Melkior Fobasso Dzeuta Aureol-Le-Rocher Ngako Njiacheu Pierre Yassa Yoniene World Health Organization Maroua Cameroon Faculty of Health Sciences University of Lisala (CIREP-UNILIS) Lisala The Democratic Republic of the Congo National Order of Medical and Sanitary Professions Yaounde Cameroon Faculty of Medicine University of Bamenda Bamenda Cameroon Faculty of Health Sciences Catholic University of Central Africa Yaounde Cameroon Faculty of Medicine University Simon Kimbangu Kinshasa The Democratic Republic of the Congo

Context/objectives: The COVID-19 pandemic has disrupted all programs, potentially impacting access to health services for children under 5 years old. The Far North of Cameroon is one of the northern regions with a prevalence of malnourished children ranging from 5% to 10%. Due to the barrier measures implemented to combat the pandemic, many parents are reluctant to take their children to healthcare facilities. Some of these children were benefiting from the Ambulatory Care Center (ACC) program prior to the COVID-19 outbreak. The main objective of this research is to assess the impact of COVID-19 on access to health services for children under 5 in the Mayo Tsanaga (MT) and Logone & Chari (LC) departments. The implementation of barrier measures could significantly increase the risk of malnutrition among children under 5 years old. Method: A cross-sectional analytical study was conducted in the MT and LC departments, employing both retrospective and prospective approaches. Data was collected through questionnaire, interview guide and a review of existing data prior to the pandemic (DHIS 2). Kobo Collect software was used for data collection, and R software was used for analysis. Results: Children who benefitted from the ACC program during the COVID-19 period were found to have a lower prevalence of malnutrition compared to those who did not benefit (OR: 0.09, CI {0.0 - 0.44}). Conclusion: COVID-19 has led to a decrease in the utilization of health facilities in both departments of the study, resulting in an increase in health issues such as malnutrition among children under 5 years old. Various policies have been implemented to improve attendance at health facilities.

关键词： COVID-19 Impact Health Child under 5 Far North Cameroon

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Eating Habits among Lithiasic Patients in Kinshasa, the Democratic Republic of Congo

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Open Journal of Urology（泌尿学期刊） 2021年第6期

作者： Pablo Kuntima Diasiama Diangienda Dieudonné Molamba Moningo Alain Ngoma Mayindu jean-Robert Rissasy Makulo Ernest Kiswaya Sumaili Eric Musalu Mafuta Mathieu Nkumu Loposso Augustin M.L.Punga-Maole simon Lwa Nkandi Lufuma jean-Philippe Haymann Michel Daudon

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HLA-G 3’UTR 14 bp Insertion Is Associated with a Decreased Risk of Developing Human African Trypanosomiasis in the Côte d’Ivoire Population

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Open Journal of Genetics 2019年第2期9卷 31-41页

作者： Bernardin Ahouty Mathurin Koffi David Courtin Ilboudo Hamidou Didier Sokouri Innocent Abé Laure Gineau Thomas Konan Lingué Kouakou Tidou Abiba Sanogo Enock Matovu Bruno Bucheton Vincent Jamonneau simon-Pierre N’Guetta Felix Houphouet Boigny University (UFHB) Cocody Abidjan C&#244 te d’Ivoire jean Lorougnon Guédé University (UJLoG) Daloa C&#244 te d’Ivoire Institut de Recherche pour le Développement (IRD) Mère et Enfant face aux Infections Tropicales Faculté de Pharmacie Université Paris Descartes Sorbonne Paris Cité France Centre International de Recherche-Développement sur l’Elevage en Zone Subhumide (CIRDES) Bobo-Dioulasso Burkina Faso Programme National d’élimination de la Trypanosomiase Humaine Africaine Abidjan C&#244 te d’Ivoire School of Veterinary Medicine Makerere University Kampala Uganda Institut de Recherche pour le Développement (IRD) IRD-CIRAD 177 Intertryp Université de Montpellier Montpellier France Institut Pierre Richet Bouaké C&#244 te d’Ivoire

Human African trypanosomiasis (HAT), or sleeping sickness, caused by Trypanosoma brucei gambiense, is associated with diverse clinical outcomes. Host’s genetic factors involved in immunity are potential factors that can regulate infection. Genetic polymorphisms within HLA-G could influence the level of HLA-G expression and therefore play a critical role in infection outcomes. The goal of our study was to investigate the association of 14 bp Indel HLA-G polymorphism with the susceptibility/resistance to HAT. DNA samples were collected from 119 cases, 221 controls and 43 seropositive individuals living in Ivorian HAT foci. The 14 bp Indel polymorphism was determined by PCR. Homozygous individuals for 14 bp insertion had a lower risk of progressing to active HAT (p = 0.012, OR = 0.27, 95% CI: 0.09 - 0.8). Moreover, the frequency of 14 bp insertion homozygous genotype was higher in the seropositive group (11%) than in the HAT cases group (3%) (p = 0.043, OR = 0.27, 95% CI: 0.07 - 0.99), which suggested a protective effect of 14 bp insertion homozygous genotype. Genetic polymorphisms in HLA-G may be associated with a variable risk to develop HAT. The 14 bp insertion appears to favour the occurrence of long-lasting T. b. gambiense latent infections.

关键词： HLA-G Human African Trypanosomiasis Trypanosoma brucei gambiense Genetic Association Study

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