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Mass Screening Using Antigenic and Molecular Diagnostic Tests of COVID-19 in Bangui at the Beginning of the Second Wave in July 2021

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Journal of Tuberculosis Research 2024年第1期12卷 1-12页

作者： Clotaire Donatien Rafaï Marie-Roseline Darnycka Belizaire pierre Somse Wilfrid Sylvain Nambei Ernest Lango-Yaya Oscar Senzongo Luc Salva Hereidebona Ulrich Jeffrey Kotemossoua Rabbi Mermoz Senekian Simon Pounguinza Christian-Diamant Mossoro-Kpinde Alain Le Faou jean De Dieu Longo François-Xavier Mbopi-Keou Gérard Grésenguet Boniface Koffi National Laboratory of Clinical Biology and Public Health Bangui Central African Republic Faculty of Health Sciences University of Bangui Bangui Central African Republic World Health Organization Bangui Central African Republic Ministry of Health and Population Bangui Central African Republic Faculty of Medicine Maieutic and Health Sciences University of Lorraine Pole Brabois Santé Nancy France Faculty of Medicine and Biomedical Sciences University of Yaounde I Yaounde Cameroun

Objective: COVID-19 surveillance was established as early as March 2020 in the Central African Republic (CAR), after the WHO statement relating to the identification of several cases outside China. However, given the non-performing molecular biology technical platform in many developing countries in sub-Saharan Africa, the second wave promised to be surprising and formidable. In this context, a mass survey was launched in Bangui to determine the prevalence of COVID-19. Patients and Methods: From March 18 to April 2, 2021, a mass screening campaign took place in tourist places, companies and the main hospital infrastructures. Nasopharyngeal swab samples were collected from participants with and without symptoms of Influenza-like illness (ILI) and stored in VTM tubes. The Ag (COVID-19) and RT-PCR tests were carried out in Bangui at the LNBCSP. The sequencing of RT-PCR SARS-CoV-2 positives was carried out at the INRB. Results: We included 1480 participants of whom 33 (2.23%) were SARS-COV-2 positive, of whom 24 were male and 9 female. This sex difference was statistically significant (p = 0.012) as the sex ratio M/F was 1.09. Sampling sites located in the 1st arrondissement were the most prolific (p = 0.006) and were sequenced. In addition to the analysis of the 33 samples from the predefined sites under study, 17 control sequences from the provinces generated during the same period are added. We detected 2 Variants Of Concern (VOC) including the predominant B.1.620 (43.86%) followed by B.1.1.7 or Alpha (5.10%). Conclusion: The study showed the importance of surveillance and the availability of means of diagnosis of COVID-19. The identified risk factors were sex and sampling site. This study has shown the importance of setting up sentinel sites for COVID-19 surveillance in all regions of the country and the appropriate use of the anti-COVID-19 vaccine.

关键词： COVID-19 Variants Central African Republic

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Enhancing laccase stability and activity for dyes decolorization using ZIF-8@MWCNT nanocomposite

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Chinese Journal of Chemical Engineering 2022年第8期35卷 66-75页

作者： Pascal Habimana Yanjun Jiang Jing Gao jean Bernard Ndayambaje Osama M.Darwesh jean pierre Mwizerwa Xiaobing Zheng Li Ma School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China University of Rwanda College of Science and Technology3900 KigaliRwanda Department of Agricultural Microbiology National Research CentreCairo 12622Egypt College of Mechatronics and Control Engineering Shenzhen UniversityShenzhen 518060China

The continuous use of chemical dyes in various industries,and their discharge into industrial effluents,results in severe problems to human life and water *** have the ability to decolorize dyes and toxic chemicals in industrial effluents as green *** possible industrial applications have been limited by poor reusability,low stability,and loss of free laccase *** this research,lac-case was immobilized on zeolitic imidazolate framework coated multi-walled carbon nanotubes(Laccase@ZIF-8@MWCNTs)via metal affinity adsorption to develop an easy separable and stable *** optimum reaction conditions for immobilized laccase are at a pH of 3.0 and a temperature of 60℃.The immobilized laccase was enhanced in storage and thermal *** results indicated that Laccase@ZIF-8@MWCNTs still maintained 68%of its original activity after 10 times of repeated *** importantly,the biocatalytic system was applied for decolorization of different dyes(20 mg·L^(-1))without a mediator,and up to 97.4%for Eriochrome black T and 95.6%Acid red 88 was achieved in 25 *** with these properties may be used in a variety of environmental and industrial applications.

关键词： Metal-organic framework Zeolitic imidazolate framework Reusability and stability Immobilized laccase Dye decolorization

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Perception of Local Populations of Bouba-Ndjidda National Park on Indigenous Knowledge and the Impact of Climate Change on Biodiversity Conservation

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Open Journal of Ecology 2023年第11期13卷 865-881页

作者： Ghislain Noé Kougoum Piebeng Samuel Christian Tsakem Kalgongbe Kiebfiene Tikela Bakwo Fils Eric Moise Simon Awafor Tamungang Michel Babale jean-pierre mate.mweru Isaac Diansambu Makanua Bouba Hotta Vincent Zoalang Mala Krossy Mavakala Raoul Sambieni Kouakou Baudouin Michel Department of Biological Sciences Faculty of Science University of Maroua Maroua Cameroon Garoua Wildlife School Garoua Cameroon Department of Forestry and Wildlife Management College of Technology University of Bamenda Bamenda Cameroon Regional Post-Graduate Training School on Integrated Management of Tropical Forests and Lands Kinshasa Democratic Repub-lic of the Congo Faculty of Science University of Kinshasa Kinshasa Democratic Republic of the Congo

This study deals with the perceptions of indigenous knowledge in the field of conservation and the impact of climate change on biodiversity in the peripheral villages of the Bouba-Ndjidda National Park (PNBN), Department of Mayo-Rey, Cameroon. To this end, 23 out of 70 villages in the study area were selected, for a sample of 368 households surveyed through a questionnaire addressed to the heads of households. Data collection took place from August 27 to December 22, 2022. The results reveal that 70.7% of the population living near the PNBN practice agriculture as their main income-generating activity, followed by livestock (23.3%) and trade (6%). Knowledge in the field of the conservation of natural resources is rooted in occultism through rituals and sacrifices (99.50%) offered to the geniuses of nature and prohibitions on the felling of trees and sacred animals (13.00%). The populations perceive climate change through the variation of the seasons (97.60%), the increase in temperature (84.80%), the reduction in the duration of the rainy seasons (54.60%), the drop in agricultural yield (84.80%) and floods (74.70%). The consequence of these changes on wildlife is the disappearance of animal species such as the Rhinoceros, the Wild Dog, the Cheetah and the Ostrich. In order to cope with climate change, the natives have developed strategies such as the adoption of early crop varieties (maize, millet, soybeans, etc.), the reorganization of the agricultural calendar, the practice of reforestation, extension of field surfaces, the cessation of excessive deforestation and uncontrolled bush fires, the construction of fired brick houses, the practice of irrigation, seasonal migration and occultism. This indigenous knowledge is a perfect adaptation to climate change and makes it possible to better take into account the vision of local populations in the conservation of biodiversity.

关键词： Perception Indigenous Knowledge Climate Change Biodiversity Conservation Bouba-Ndjidda National Park

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The value does not exist!A motivation for extremal analysis

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Probability, Uncertainty and Quantitative Risk 2022年第3期7卷 195-214页

作者： jean-pierre Aubin Hélène Frankowska Retired 14Rue Domat75005 ParisFrance CNRS IMJ-PRGSorbonne Université4Place Jussieu75252 ParisFrance

Standard mathematical economics studies the production,exchange,and consumption of goods“provided with units of measurement,”as in physics,in order to be enumerated,quantified,added,***,“baskets of goods,”which should describe subsets of goods,are mathematically represented as commodity vectors of a vector space,linear combination of units of goods,evaluated by prices,which are linear numerical ***,in this sense,mathematical economics is a branch of ***,economics,and many other domains of life sciences,investigate also what will be called entities,defining elements deprived of units of measure,which thus cannot be enumerated.(1)Denoting by X the set of entities x∈X deprived of units of measurement,a“basket of goods”is actually a subset K■X of the set entities,i.e.,an element of the“hyperset”P(X),the family of subsets of X,and no longer a commodity vector of the vector space of commodities;(2)Entities can be“gathered”instead of being“added”;(3)Entities can still be evaluated by a family of functions A:x 2 X 7!A(x)2 Rregarded as a“valuators,”in lieu and place of linear“prices”evaluating the units of economic goods.(4)Subsets of entities can be evaluated by an“interval of values”between two extremal ones,the minimum and the maximum,instead of the sum of values of units of goods weighted by their *** sciences dealing with intertwined relations among many combinations of entities,hypersets offer metaphors of“Lamarckian complexity”that keeps us away from binary relations,graphs of functions,and set-valued maps,to focus our attention on“multinary relations”between families of *** deprived of units of measurement,these“proletarian”entities still enjoy enough properties for this pauperization to be mathematically *** is the object of this extremal manifesto:in economics and other domains of life sciences,vector spaces should yield their imperial status of“state space”to hypersets and linear prices to hy

关键词： Valuators Extremal topologies Transfer of topologies Confined sets Extremal evolution Inert relation Viability kernel Regulator

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Application of near-infrared spectroscopy for fast germplasm analysis and classification in multi-environment using intact-seed peanut(Arachis hypogaea L.)

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Oil Crop Science 2024年第2期9卷 132-141页

作者： Fentanesh Chekole Kassie Gilles Chaix Hermine Bille Ngalle Maguette Seye Coura Fall Hodo-Abalo Tossim Aissatou Sambou Olivier Gibert Fabrice Davrieux Joseph Martin Bell jean-Francois Rami Daniel Fonceka Joel Romaric Nguepjop Department of Plant Biology and Physiology Faculty of SciencesUniversity of Yaounde IYaoundeP.O.Box 337Cameroon Department of Plant Science College of AgricultureWolaita Sodo UniversitySodoP.O.Box 138Ethiopia CIRAD UMR AGAP InstitutMontpellierFrance AGAP Institut Univ MontpellierCIRADINRAEInstitut AgroMontpellierFrance ChemHouse Research Group MontpellierFrance Centre D’Etudes Regional Pour L’Amelioration de L’Adaptation a La Secheresse(CERAAS/ISRA) Route de KhomboleThiesBP 3320Senegal Dispositif de Recherche et de Formation en Partenariat Innovation et Amelioration Varietale en Afrique de L’Ouest(IAVAO)CERAASRoute de KhomboleThiesBP 3320Senegal CIRAD UMR QUALISUDSaint PierreFrance

Peanut is a worldwide oilseed crop and the need to assess germplasm in a non-destructive manner is important for seed nutritional *** this study,Near Infrared Spectroscopy(NIRS)was applied to rapidly assess germplasm variability from whole seed of 699 samples,field-collected and assembled in four genetic and environmentbased sets:one set of 300 varieties of a core-collection and three sets of 133 genotypes of an interspecific population,evaluated in three environments in a large spatial scale of two countries,Mbalmayo and Bafia in Cameroon and Nioro in Senegal,under rainfed *** elemental spectra were gathered on six subsets of seeds of each sample,after three rotation scans,with a spectral resolution of 16 cm-1over the spectral range of867 nm to 2530 *** were then processed by principal component analysis(PCA)coupled with Partial least squares-discriminant analysis(PLS-DA).As results,a huge variability was found between varieties and genotypes for all NIR wavelength within and between *** magnitude of genetic variation was particularly observed at 11 relevant wavelengths such as 1723 nm,usually related to oil content and fatty acid *** yielded the most chemical attributes in three significant PCs(i.e.,eigenvalues>10),which together captured 93%of the total variation,revealing genetic and environment structure of varieties and genotypes into four clusters,corresponding to the four samples *** pattern of genetic variability of the interspecific population covers,remarkably half of spectrum of the core-collection,turning out to be the ***,a PLS-DA model was developed and a strong accuracy of 99.6%was achieved for the four sets,aiming to classify each seed sample according to environment *** confusion matrix achieved for the two sets of Bafia and Nioro showed 100%of instances classified correctly with 100%at both sensitivity and specificity,confirming that their seed quality was different f

关键词： Groundnut Oilseed Near infrared spectroscopy Germplasm analysis Environment Nutritional Breeding

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