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A Dialogue between the Sirene Pathway in Synergids and the Fertilization Independent Seed Pathway in the Central Cell Controls Male Gamete Release during Double Fertilization in Arabidopsis

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Molecular Plant 2008年第4期1卷 659-666页

作者： Nicolas Rotman Mathieu Gourgues Anne-Elisabeth Guitton jean-emmanuel faure Frederic Berger Ecole normale superieure de Lyon Unite Mixte de Recherches 5667 Reproduction et d~veloppement des plantes 46 allee d'ltalie 69007 Lyon cedex 07France Centre for Integrative Genomics University of Lausanne Le Genopode 1015 Lausanne Switzerland Unite Mixte de Recherches IPMSV CNRSIINRA/Unice 400 route des chappes Sophia Antipolis F-06903 France European Commission Research Directorate General Research Infrastructures iUnit B3) Office SDME 1/133 B-1049 Brussels Belgium Temasek Life Sciences Laboratory 1 Research link National University of Singapore Singapore 117604

Angiosperms sexual reproduction involves interactions between the two female gametes in the embryo sac and the two male gametes released by the pollen tube. The two synergids of the embryo sac express the FERONIA/SIRENE receptor-like kinase, which controls the discharge of the two sperm cells from the pollen tube. FER/SRN may respond to a ligand from the pollen tube. Alternatively, the interaction between FER/SRN and a ligand from the embryo sac may lead to a state of competence of the synergids allowing pollen tube discharge. Here, we report the new mutant scylla （syl） impaired in the control of pollen tube discharge. This mutant also produces autonomous endosperm development in ab- sence of fertilization--a trait associated with the FERTILIZATION INDEPENDENT SEED （FIS） mutant class. This led us to iden- tify autonomous endosperm in srn mutants and to demonstrate synergistic interactions between srn and the fis mutants. In addition, the fis mutants display defects in pollen tube discharge as in srn and syl mutants, confirming the interaction between the two pathways. Our findings suggest that pollen tube discharge is controlled by an interaction between the synergids expressing SRN/FER and the central cell expressing FIS genes.

关键词： FERONIA/SIRèNE FER/SRN

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Density Results in Sobolev Spaces Whose Elements Vanish on a Part of the Boundary

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Chinese Annals of Mathematics,Series B 2011年第6期32卷 823-846页

作者： jean-Marie emmanuel BERNARD Laboratoire Analyse et Probabitilités Université d'Evry val d'Essonne23 Boulevard de France91037 EVRYFrance

This paper is devoted to the study of the subspace of Wm＇＂ of functions that vanish on a part γ0 of the boundary. The author gives a crucial estimate of the Poincare constant in balls centered on the boundary of γ0. Then, the convolution-translation method, a variant of the standard mollifier technique, can be used to prove the density of smooth functions that vanish in a neighborhood of γ0, in this subspace. The result is first proved for m = 1, then generalized to the case where m 〉 1, in any dimension, in the framework of Lipschitz-continuous domain. However, as may be expected, it is needed to make additional assumptions on the boundary of γ0, namely that it is locally the graph of some Lipschitz-continuous function.

关键词： Density results Boundary value problems Sobolev spaces

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A unified electrical model based on experimental data to describe electrical transport in carbon nanotube-based materials

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Nano Research 2020年第6期13卷 1764-1779页

作者： Yoann Dini Jérôme faure-Vincent jean Dijon Univ.Grenoble-Alpes CEALITENDTNMF-38000GrenobleFrance Univ.Grenoble-Alpes CEACNRSIRIGSyMMESF-38000GrenobleFrance

Understanding the electrical transport in carbon nanotube(CNT)materials is one key to reach very high electrical *** CNT material resistivity(ρ(T))as function of the temperature are fully apprehended by their reduced activation W(T)=dln(ρ)/dln(T)*** to now,no model accurately fits W(T)curves,thus preventing from precisely describing the CNT material electrical *** present a new electrical transport model that perfectly fits all W(T)curves found in the literature and in our own *** material resistivities are modeled byρ(T)=ρ0(T^−α+M(1+βTγT^2)).Our model has few enough parameters(α,M,β,γ)to relate them to the CNT *** 70 K,we experimentally show that CNT material resistivity follows the Luttinger Liquid theory justifying the T^−αterm in our *** 70 K,the polynomial part becomes dominant and depends on the two different CNT fabrication techniques which lead to two very different yarn *** yarns made from floating catalyst chemical vapor deposition CNTs,the polynomial is explained by the percolation of metallic CNT ***,the polynomial of yarns spun from CNT arrays is explained by the electrical transport in CNT bundles which are the basic building blocks of this type of yarns.

关键词： carbon nanotube carbon-based materials electrical conductivity electrical transport model Luttinger Liquid

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Wear Behavior of a Bio-inspired Bearing for off-center Loads

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Journal of Bionic Engineering 2020年第6期17卷 1251-1262页

作者： Delphine Sysaykeo jean-Marc Linares emmanuel Mermoz Alix-Marseile Universite CNRSCentre National de la Recherche ScientifiqueISMInstitut des Sciences du MouvementMarseille 13009France Advanced concepts and Dynamic systems architecture department Airbus HelicoptersAeroport International Marseille ProvenceMarignane 13700France

Misalignment is one of the most common causes of wear in bush *** improvements have been proposed by many ***,it did not efficiently reduce the *** geometrical designs sometimes reach their *** this reason,a bio-inspired design is proposed to solve the *** this article,a bio-inspired bearing suited to misalignment was tested and compared to a classical bush *** contact pressures of both bearings were compared with static Finite Element(FE)simulations for off-center *** to the complex shape of the involved contact,the performances of both bearings were also studied over *** wear behaviors were predicted with a numerical *** methodologies emplaced to simulate the wear were described in this ***,the wear coefficient determination obtained by experimental testing was *** pressure value,the contact zone and the wear depth were compared and *** wear results for the classical bearing are in accordance with the *** simulations show a deeper wear on the classical bush bearing than on the bio-inspired *** leads to a longer period of service life for the bio-inspired bearing.

关键词： biologically inspired design bush bearing wear simulation

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Joint Loading Estimation Method for Horse Forelimb High Jerk Locomotion:Jumping

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Journal of Bionic Engineering 2019年第4期16卷 674-685页

作者： Joanne Becker Mermoz emmanuel Linares jean-Marc Airbus Helicopters Aeroport de Marseille ProvenceMarignane 13700France Aix Marseille Univ CNRSISMInst Movement SciMarseille 13009France

Maximal local loads in animal joints are necessary to design bio-inspired mechanical *** studies presented methods to determine joint reaction forces in humans and ***,many of these methods are invasive,and no work has been published yet about the joint reaction forces in the horse forelimb during ***-invasive methods to measure the kinematics and ground reaction force of a horse forelimb were used in this work.A musculoskeletal model of horse forelimb was built with mechanical methods for the estimation of joint reaction *** entire forelimb was reconstructed by scanning real bones geometry with a 3D optical scanner and modeling all the muscles on a Computer Assisted Design(CAD)*** model dynamics were simulated with OpenSim in order to estimate the joint *** study allows knowing an order of magnitude of the loads at the joints at jumping in order to determine latter the maximal joint contact loading values that will be a key at designing bio-inspired joints for mechanical assemblies.

关键词： joint loading jumping kinematics modelling OpenSim

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Bio-inspired Topological Skeleton for the Analysis of Quadruped Kinematic Gait

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Journal of Bionic Engineering 2018年第5期15卷 839-850页

作者： Benjamin Boudon jean-Marc Linares Anick Abourachid Arthur Vauquelin emmanuel Mermoz Aix Marseille University CNRS ISM Marseille 13009 France Museum National d'Histoire Naturelle Pavillon d'Anatomie Comparbe 57 rue Cuvier F-75231 Paris Cedex 05 France Airbus Helicopters Abroport de Marseille Provence Marignane 13700 France

In bio-inspired design activities, nature is a basis of knowledge. Over the last twenty years, many solutions to measure and analyze human or animal gaits have been developed （VICON system, X-ray radiography...）. Although, these methods are becoming more and more accurate, they are quite expensive, long to set up and not easily portable. In this paper, a method called the bio-inspired topological skeleton is proposed in order to complement the classic videography process and to enable animal gait analysis. A new predictive kinematic model with closed-loops of an unguligrade quadruped is suggested. This kinematic model includes three segments per leg and takes into account the scapula movements. The proposed method allows us to improve the accuracy of the kinematic input data measured from a single video including an additional artefact. To show the benefits of this method, joint parameters that are difficult to measure are derived symbolically from the kinematic model and compared with experimental data.

关键词： quadruped locomotion motion analysis topological skeleton closed-form solutions Multibody Systems (MBS)

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Circulating tumor cell isolation:the assets of filtration methods with polycarbonate track-etched filters

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Chinese Journal of Cancer Research 2015年第5期27卷 479-487页

作者： Claire Dolfus Nicolas Piton emmanuel Toure jean-Christophe Sabourin Department of Pathology Rouen University Hospital Inserm U1079 Institute for Biomedical Research and InnovationUniversity of Rouen

Circulating tumor cells （CTCs） arise from primary or secondary tumors and enter the bloodstream by active or passive intravasation. Given the low number of CTCs, enrichment is necessary for detection. Filtration methods are based on selection of CTCs by size using a filter with 6.5 to 8 pm pores. After coloration, collected CTCs are evaluated according to morphological criteria. Immunophenotyping and fluorescence in situ hybridization techniques may be used. Selected CTCs can also be cultivated in vitro to provide more material. Analysis of genomic mutations is difficult because it requires adapted techniques due to limited DNA materials. Filtration-selected CTCs have shown prognostic value in many studies but multicentric validating trials are mandatory to strengthen this assessment. Other clinical applications are promising such as follow-up, therapy response prediction and diagnosis. Microfluidic emerging systems could optimize filtration-selected CTCs by increasing selection accuracy.

关键词： Circulating tumor ceils (CTCs) biological markers filtration

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Petrogenesis of the Mayo-Darley tin formations,anorogenic complex of the Cameroon Line:implication for tin deposit

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Acta Geochimica 2023年第4期42卷 704-725页

作者： Ndema Mbongué jean Lavenir Ndongfong Edson Fuh emmanuel Eseya Mengu Junior Department of Geology Faculty of SciencesUniversity of BueaP.O.Box 63BueaCameroon Department of Earth Sciences Faculty of SciencesUniversity of Yaounde IP.O.Box 812YaoundéCameroon

The Mayo-Darley massif is an anorogenic complex of the Cameroon Line that has a mantle ***-bearing rocks were analyzed by ICP-AES and INAA analytical *** purpose of this work was to provide new geochemical data for the Mayo-Darley tin formation and to understand its petrogenesis and the origin of tin *** Mayo-Darley tin deposit is made up of tin-granite,and tin-greisen,greisenification was developed on the borders of quartz *** rocks belong to the alkaline series and were classified into acid(SiO2-=61.6%–73.8%;65.4%–98%respectively)and basic(42.9%–47%SiO_(2))*** showed enrichment in HSFE,LILE,Ga/Al and chondrite normalized REE patterns indicating LREE enrichment relative to HREE with a negative Eu anomaly,only sample SB8 of tin-granite showed Eu/Eu*=*** display metaluminous,peralkaline,peraluminous,ferroan,high-K calc-alkaline to shoshonitic,alkalic to calcic affinity,and crystallized at800°*** chemistry of this deposit reflects the primary composition of granite,quartz monzonite,gabbro,and foid *** complex experienced multi-stage sub-solidus hydrothermal fluid reactions and shows variable alteration of feldspars alkali *** rocks are classified as A1-type granite,overlap with the OIB field,and were derived from a within-plate setting,similar to mantle non plumederived *** origin of tin mineralization in MayoDarley has a complex evolution,tin mineralization was derived from hydrothermal and hydrogenous metal-rich deposits and shows Sn–Hf–Zr and Sn–Tl–Nb *** new data confirm the complexity of the Mayo-Darley tin complex and elucidate the origin of tin mineralization.

关键词： Mayo-Darley Massif Cameroon Line Tingranite Tin-bearing greisen A1-type granite Sn–Hf–Zr Sn–Tl–Nb

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Use of a new retrograde filling material(Biodentine) for endodontic surgery: two case reports

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International Journal of Oral Science 2014年第4期6卷 250-253页

作者： Grégory Caron jean Azérad Marie-Odile faure Pierre Machtou Yves Boucher Private practice Dental School Paris Diderot University Dental Service Pitié Salpêtrière Hospital Septodont Saint-Maur-des-Fosses

Mineral trioxide aggregate（MTA） is considered at the present time as the gold standard for root-end filling in endodontic ***, this biocompatible material presents several drawbacks such as a long setting time and handling difficulties. The aim of this article is to present a new commercialized calcium silicate-based material named Biodentine with physical improved properties compared to MTA in a clinical application. Two endodontic microsurgeries were performed by using specific armamentarium（microsurgical instrumentation, ultrasonic tips） under high-power magnification with an operatory microscope. Biodentine was used as a root-end filling in order to seal the root canal system. The two cases were considered completely healed at 1 year and were followed for one more year. The 2-year follow-up consolidated the previous observation with absence of clinical symptoms and radiographic evidence of regeneration of the periapical tissues.

关键词： Biodentin endodontics healing periapical surgery retrograde filling

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Benefits of jejunostomy feeding in patients who underwent gastrectomy for cancer treatment

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World Journal of Gastrointestinal Surgery 2024年第8期16卷 2461-2473页

作者： Romain Jaquet emmanuel Rivkine Nicole De Souza jean Roudié Department of Digestive and Visceral Surgery Nord Essonne Hospital Group-Longjumeau SiteLongjumeau 91160France Department of General and Digestive Surgery Pierre Zobda-Quitman HospitalUniversity Hospital CenterMartiniqueFranceFort de France 97261Martinique

BACKGROUND Gastric cancer is associated with significant undernutrition responsible for an increase in morbidity and mortality after *** To evaluate the impact of enteral nutrition by jejunostomy feeding in patients undergoing gastrectomy for *** Between 2003 and 2017,all patients undergoing gastrectomy for cancer treatment were included retrospectively.A group with jejunostomy(J+group)and a group without jejunostomy(J-group)were *** Of the 172 patients included,60 received ***,the two groups were comparable with respect to the nutritional parameters studied(body mass index,albumin,etc.).In the postoperative period,the J+group lost less weight and albumin:5.74±8.4 vs 9.86±7.5 kg(P=0.07)and 7.2±5.6 vs 14.7±12.7 g/L(P=0.16),*** morbidity was 25%in the J+group and 36.6%in the J-group(P=0.12).The J+group had fewer respiratory,infectious,and grade 3 complications:0%vs 5.4%(P=0.09),1.2%vs 9.3%(P=0.03),and 0%vs 4.7%(P=0.05),*** 30-day mortality was 6.7%in the J+group and 6.3%in the J-group(P=0.91).CONCLUSION Jejunostomy feeding after gastrectomy improves nutritional characteristics and decreases postoperative morbidity.A prospective study could confirm our results.

关键词： Feeding jejunostomy Gastric cancer Undernutrition Morbimortality Survival

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