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Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nicotiana tabacum

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Science Bulletin 2022年第3期67卷 315-327页

作者： Chen Dong Ge Qu Jinggong Guo Fang Wei Shuwen Gao Zhoutong Sun Lifeng Jin Xuwu Sun jean-david rochaix Yuchen Miao Ran Wang Zhengzhou Tobacco Research Institute Zhengzhou 450001China Key Laboratory of Plant Stress Biology State Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475001China College of Biological Engineering Henan University of TechnologyZhengzhou 450001China Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin 300308China School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001China Departments of Molecular Biology and Plant Biology University of GenevaGeneva 1211Switzerland

Restricted genetic diversity can supply only a limited number of elite genes for modern plant cultivation and *** this study,we demonstrate that rational design enables the engineering of geranyl-geranyl diphosphate synthase(NtGGPPS),an enzyme of the methylerythritol phosphate pathway(MEP)in the model plant Nicotiana *** the crucial bottleneck in carotenoid biosynthesis,NtGGPPS1 interacts with phytoene synthase(NtPSY1)to channel GGPP into the production of *** of this enzyme in the ntggpps1 mutant leads to decreased carotenoid *** the aim of enhanc-ing NtGGPPS1 activity,we undertook structure-guided rational redesign of its substrate binding pocket in combination with sequence *** activity of the designed NtGGPPS1(a pentuple mutant of five sites V154A/I161L/F218Y/I209S/V233E,d-NtGGPPS1)was measured by a high-throughput colorimetric assay.d-NtGGPPS1 exhibited significantly higher conversion of IPP and each co-substrate(DMAPP~1995.5-fold,GPP~25.9-fold,and FPP~16.7-fold)for GGPP synthesis compared with wild-type ***,the transient and stable expression of d-NtGGPPS1 in the ntggpps1 mutant increased carotenoid levels in leaves,improved photosynthetic efficiency,and increased biomass relative to *** findings provide a firm basis for the engineering of GGPPS and will facilitate the development of quality and yield *** results open the door for the structure-guided rational design of elite genes in higher plants。

关键词： Rational design Carotenoid Methylerythritol phosphate pathway(MEP) Geranylgeranyl diphosphate synthase(GGPPS) Nicotiana tabacum

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Retrograde Signaling and Photoprotection in a gun4 Mutant of Chlamydomonas reinhardtii

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Molecular Plant 2012年第6期5卷 1242-1262页

作者： Cinzia Formighieri Mauro Ceol Giulia Bonente jean-david rochaix Roberto Bassi Dipartimento di Biotecnologie Universita di Verona Strada Le Grazie 15 1-37134 Verona Italy Departments of Molecular Biology and Plant Biology University of Geneva Sciences Ⅲ 30 Quai Ernest-Ansermet CH-1211 Geneva 4 Switzerland

GUN4 is a regulatory subunit of Mg-chelatase involved in the control of tetrapyrrole synthesis in plants and cyanobacteria. Here, we report the first characterization of a gun4 insertion mutant of the unicellular green alga Chlamydomonas reinhardtii. The mutant contains 50% of chlorophyll as compared to wild-type and accumulates ProtolX. In contrast to the increase in LHCtranscription, the accumulation of most LHC proteins is drastically diminished, implying posttranscriptional down-regulation in the absence of transcriptional coordination. We found that 803 genes change their expression level in gun4 as compared to wild-type, by RNA-Seq, and this wide-ranging effect on transcription is apparent under physiological conditions. Besides LHCs, we identified transcripts encoding enzymes of the tetrapyrrole pathway and factors involved in signal transduction, transcription, and chromatin remodeling. Moreover, we observe perturbations in electron transport with a strongly decreased PSI-to-PSII ratio. This is accompanied by an enhanced activ- ity of the plastid terminal oxidase （PTOX） that could have a physiological role in decreasing photosystem II excitation pressure.

关键词： green algae Chlamydomonas genome uncoupled (gun) mutants gun4 retrograde signaling PTOX.

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Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution

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Journal of Integrative Plant Biology 2016年第12期58卷 943-946页

作者： Mei Chen Jin Zhang Lei Zhao Jiale Xing Lianwei Peng Tingyun Kuang jean-david rochaix Fang Huang Key Laboratory of Photobiology Institute of Botanythe Chinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China Departments of Molecular Biology and Plant Biology University of GenevaSwitzerland

Summary We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 （PGRS） capable of producing hydrogen （H2） for 25 days with more than 3o-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H2 production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H2 production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species （ROS） scavenging capacity during sulfur depriva- tion. This anti-ROS response appears to protect the photosynthetic electron transport chain from photo- oxidative damage and thereby ensures electron supply to the hydrogenase.

关键词： Chlamydomonas reinhardtii hpm91 hydrogen photoproduction oxidative stress photosystem II sulfur deprivation

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Mitochondrial recruitment in myelin:an anchor for myelin dynamics and plasticity?

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Neural Regeneration Research 2024年第7期19卷 1401-1402页

作者： jean-david M.Gothié Timothy E.Kennedy Department of Neurology and Neurosurgery Montreal Neurological InstituteMcGill UniversityMontrealQCCanada

Optimal propagation of neuronal electrical impulses depends on the insulation of axons by myelin,produced in the central nervous system by *** is an extension of the oligodendrocyte plasma membrane,which wraps around an axon to form a compact multi-layered *** is composed of a substantially higher proportion of lipids compared to other biological membranes and enriched in a small number of specialized proteins.

关键词： plasticity insulation dynamics

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Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing

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Molecular Plant 2020年第8期13卷 1178-1193页

作者： Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing Sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo jean-david rochaix Yuchen Miao Xuwu Sun State Key Laboratory of Crop Stress Adaptation and Improvement State Key Laboratory of Cotton BiologyKey Laboratory of Plant Stress BiologySchool of Life SciencesHenan University85 Minglun StreetKaifeng 475001China College of Life Sciences Shanghai Normal UniversityGuilin Road 100Shanghai200234China Departments of Molecular Biology and Plant Biology University of GenevaGeneva1211Switzerland

The regulation of stomatal lineage cell development has been extensively ***,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been *** this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis *** identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker *** analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother *** of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these ***,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development.

关键词： molecular profiling stomatal development single-cell RNA-seq

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High frequency of severe liver dysfunction in critically ill Dengue patients in the French West Indies

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Journal of Intensive Medicine 2024年第3期4卷 403-404页

作者： Laurent Camous Samuel Markowicz Cecile Loraux Julien Jabot jean david Pommier Réanimation Médicale et Chirurgicale CHU de GuadeloupeChemin ChauvelLes Abymes97139 GuadeloupeFrance Service de Maladies infectieuses et tropicales CHU de GuadeloupeChemin ChauvelLes Abymes97139 GuadeloupeFrance Service de Microbiologie CHU de GuadeloupeChemin ChauvelLes Abymes97139 GuadeloupeFrance Médecine Intensive et Réanimation Centre hospitalier universitaire de la Réunion 97460 Saint-PaulFrance Institut Pasteur de la Guadeloupe Morne Jolivière97183 AbymesFrance

Dear Editor,Dengue is a worldwide zoonosis,transmitted by mosquitoes and highly prevalent in tropical and subtropical *** recent years,its incidence has increased with a massive healthcare burden worldwide[1]and recent climatic changes have expanded the geographic range of the insect vector with described outbreaks in Europe.[2]Most patients present pauci-symptomatic infections,but some patients could present severe manifestations with high reported mortality[[3],[4],[5]]and no specific *** bleeding,hemodynamic failure and various organ damage(cardiac,renal,neurological,and hepatic)are described in severe dengue(SD)patients.[5]In the future,intensivists worldwide will be increasingly treating SD and the spectrum of severe organ impairment should be well known.

关键词： patients mortality expanded

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An acoustofluidic device for the automated separation of platelet-reduced plasma from whole blood

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Microsystems & Nanoengineering 2024年第3期10卷 293-303页

作者： Zhehan Ma Jianping Xia Neil Upreti Emeraghi david Joseph Rufo Yuyang Gu Kaichun Yang Shujie Yang Xiangchen Xu jean Kwun Eileen Chambers Tony Jun Huang Thomas Lord Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNCUSA Department of Biomedical Engineering Duke UniversityDurhamNCUSA Department of Pediatrics Duke UniversityDurhamNCUSA Duke Transplant Center Department of SurgeryDuke University School of MedicineDurhamNCUSA

Separating plasma from whole blood is an important sample processing technique required for fundamental biomedical research,medical diagnostics,and therapeutic *** protocols for plasma isolation require multiple centrifugation steps or multiunit microfluidic processing to sequentially remove large red blood cells(RBCs)and white blood cells(WBCs),followed by the removal of small ***,we present an acoustofluidic platform capable of efficiently removing RBCs,WBCs,and platelets from whole blood in a single *** leveraging differences in the acoustic impedances of fluids,our device generates significantly greater forces on suspended particles than conventional microfluidic approaches,enabling the removal of both large blood cells and smaller platelets in a single *** a result,undiluted human whole blood can be processed by our device to remove both blood cells and platelets(>90%)at low voltages(25 Vpp).The ability to successfully remove blood cells and platelets from plasma without altering the properties of the proteins and antibodies present creates numerous potential applications for our platform in biomedical research,as well as plasma-based diagnostics and ***,the microfluidic nature of our device offers advantages such as portability,cost efficiency,and the ability to process small-volume samples.

关键词： remove separation removing

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Macroalgal deep genomics illuminate multiple paths to aquatic,photosynthetic multicellularity

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Molecular Plant 2024年第5期17卷 747-771页

作者： david R.Nelson Alexandra Mystikou Ashish Jaiswal Cecilia Rad-Menendez Michael J.Preston Frederik De Boever Diana C.El Assal Sarah Daakour Michael W.Lomas jean-Claude Twizere david H.Green William C.Ratcliff Kourosh Salehi-Ashtiani Division of Science and Math New York University Abu DhabiAbu DhabiUAE Center for Genomics and Systems Biology(CGSB) New York University Abu DhabiAbu DhabiUAE Biotechnology Research Center Technology Innovation InstitutePO Box 9639Masdar CityAbu DhabiUAE Culture Collection of Algae and Protozoa Scottish Association for Marine ScienceObanScotlandUK National Center for Marine Algae and Microbiota Bigelow Laboratory for Ocean SciencesEast BoothbayMEUSA Laboratory of Viral Interactomes GIGA InstituteUniversity of LiegeLiegeBelgium School of Biological Sciences Georgia Institute of TechnologyAtlantaGAUSA

Macroalgae are multicellular,aquatic autotrophs that play vital roles in global climate maintenance and have diverse applications in biotechnology and eco-engineering,which are directly linked to their multicellularity ***,their genomic diversity and the evolutionary mechanisms underlying multicellularity in these organisms remain *** this study,we sequenced 110 macroalgal genomes from diverse climates and phyla,and identified key genomic features that distinguish them from their microalgal *** for cell adhesion,extracellular matrix formation,cell polarity,transport,and cell differentiation distinguish macroalgae from microalgae across all three major phyla,constituting conserved and unique gene sets supporting multicellular *** genes show phylum-and climate-specific expansions that may facilitate niche ***,our study reveals genetic determinants of convergent and divergent evolutionary trajectories that have shaped morphological diversity in macroalgae and provides genome-wide frameworks to understand photosynthetic multicellular evolution in aquatic environments.

关键词： macroalgae multicellularity comparative genomics evolution adhesome endogenous viral elements

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Cardiac Tolerance of Hydroalcoholic Extract of Bark of Terminalia mantaly H. Perrier (HAEBTM) in Wistar Rats

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American Journal of Molecular Biology 2024年第3期14卷 126-137页

作者： Irié Lou Bohila Emilie Kamo Kouakou Serge Kouassi Virginie Atto Allico Joseph Djaman jean david N’guessan Mireille Dosso Department of Microbiology Institut Pasteur of Cte dIvoire Abidjan Cte dIvoire Biology and Health Laboratory Flix Houphout-Boigny University Abidjan Cte dIvoire Department of Medical and Fundamental Biochemistry Institut Pasteur of Cte dIvoire Abidjan Cte dIvoire

Terminalia mantaly H. Perrier is a plant used in traditional medicine for the treatment of various pathologies. However, Terminalia mantaly H. Perrier could present potential health effects on patients. In order to determine the possible cardiotoxic effects of the hydro-alcoholic extract of the bark of Terminalia mantaly H. Perrier, (HAEBTM) forty (40) rats distributed randomly into 4 groups, including 10 animals per group (5 males and 5 females) were used. Animals in group 1 received distilled water and were used as a control group. On the other hand, groups 2, 3, 4 received oral administration a volume of the hydroalcoholic extract of Terminalia mantaly H. Perrier corresponding to 1 mL/100g of body weight at 150 mg/kg, 300 mg/kg, 600 mg/kg, respectively. The extract was administered daily at the same time for 28 days and serum was collected once a week to evaluate cardiac biochemical markers using spectrophotometric methods using a Cobas C311 HITACHI biochemistry system. After one month of study, all rats were euthanized by overdose of ether, and the hearts of the rats were removed for gross morphological and histopathological analysis. Results were analysed using variance analysis (ANOVA) to compare outcomes as a function of doses administered and treatment times. The biochemical parameters ALT, LDH, CPK, CPKMB showed no significant change (p Terminalia mantaly showed no lesions, edema and necrosis. These results suggest that the hydroalcoholic extract of Terminalia mantaly did not interfere with the functioning or alter the integrity of the heart.

关键词： Terminalia mantaly Bio Cardiac Tolerance Biochemical Markers Histopathology

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Molecular Characterization of Gastrointestinal Stromal Tumors (Gist) and Contribution of Immunohistochemistry in Congolese from Kinshasa

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Open Journal of Gastroenterology 2024年第4期14卷 109-116页

作者： Rive Lukuaku Jérémie Okumadi Fabrice Bokambandja Andy Mbangama Vincent Mbatu Georges Mvumbi jean-Louis Mumbere david Azako Serge Pezo Dieudonné Mvumbi Faculty of Medicine Bel Campus Technological University Kinshasa DR Congo Department of Anatomy and Pathological Cytology University Clinics of Kinshasa Kinshasa DR Congo Department of Gyneco-Obstetrics University Clinics of Kinshasa Kinshasa DR Congo Molecular Biology Department Faculty of Medicine University of Kinshasa Kinshasa DR Congo Institute of Medical Immunology Free University of Brussels Ixelles Belgium

Introduction: The differentiation of digestive tumors very often requires the use of techniques currently not widely in use in the Democratic Republic of Congo (DRC), such as immunohistochemistry. This is perfectly verified for GISTs whose precise, or at least highly certain, diagnosis can only be made using immunohistochemical markers. This underuse of these techniques due to lack of equipment and human skills explains the limited epidemiological data available to date, thus leading to untargeted and too often late treatment of patients. Research question: What contribution can immunohistochemical markers make to the diagnosis of digestive tract tumours? Objective: Discuss the contribution of immunohistochemical markers in the diagnosis of GIST and provide basic data on the epidemiology of these nosological entities in Kinshasa. Methodology: This was a retrospective study carried out at the LEBOMA private anatomy and pathological cytology centre. The main inclusion criterion was any digestive tract block or slide whose diagnosis of GIST had been requalified after review by at least 2 pathologists. An immuhistochemical study was performed using an automated technique (with a Ventana XT machine) using a panel of antibodies: CD-117 and DOG-1 which are listed in the literature as strongly correlated with the occurrence of GIST, all slides were made at Hj Hospital using an OLYMPUS BX41 co-observation microscope. Results: Of 601 cases of digestive tumors recorded during the concerned period, 32 (5.32%) concerned GIST. This prevalence was confirmed by our immunohistochemical results where the expression of CD117 and that of DOG-1 were positive in 90.6% and 100% of cases which prevalence is high compared with the worldwide prevalence according to the literature, respectively. The distribution of the patients concerned was made with a sex ratio of 1.6 women/men with a median age of 53 years. Most cases (81%) had a gastric location and were fusiform GISTs. Conclusion: Gastro

关键词： Digestive Tumors GIST Immunohistochemistry CD117 DOG-1

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