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New Strigolactone Analogs as Plant Hormones with Low Activities in the Rhizosphere

Molecular Plant 2014年第4期7卷 675-690页

作者： Francois-Didier Boyer Alexandre de Saint Germain jean-bernard pouvreau Guillaume Clave jean-Paul Pillot Amelie Roux Amanda Rasmussen Stephen Depuydt Dominique Lauresserguesh Nicolas Frei dit Frey Thomas S.A. Heugebaert Christian V. Stevens Danny Geelen Sofie Goormachtig Catherine Rameau Institut jean-Pierre Bourgin UMR1318 Institut National de la Recherche Agronomique (INRA)-AgroParisTech Route de Saint-Cyr (RD 10) F-78026 Versailles Cedex France Centre de Recherche de Gif Institut de Chimie des Substances Naturelles UPR2301 Centre National de la Recherche Scientifique (CNRS) 1 avenue de la Terrasse F-91198 Gif-sur-Yvette Cedex France Laboratoire de Biologie et de Pathologie Vegetales EA 1157 SFR 4207 QUASAV Universite de Nantes 44322 Nantes France Plant Production Faculty of Bioscience Engineering Ghent University Coupure links 653 Ghent 9000 Belgium Present address: School of Biosciences Division of Plant and Crop Production The University of Nottingham Sutton Bonington Leicestershire LE12 5RD UK Department of Plant Systems Biology VIB Ghent 9052 Belgium Department of Plant Biotechnology and Bioinformatics Ghent University Ghent 9052 Belgium Laboratoire de Recherche en Sciences Vegetales UMR 5546 Universite de Toulouse- Centre National de la Recherche Scientifique (CNRS) BP 42617 Auzeville F-31326 Castanet-Tolosan France Research Group SynBioC Department of Sustainable Organic Chemistry and Technology Faculty of Bioscience Engineering Ghent University Coupure links 653 B-9000 Gent Belgium

Strigolactones （SLs） are known not only as plant hormones, but also as rhizosphere signals for establishing symbiotic and parasitic interactions. The design of new specific SL analogs is a challenging goal in understanding the basic plant biology and is also useful to control plant architectures without favoring the development of parasitic plants. Two different molecules （23 （3＇-methyI-GR24）, 31 （thia-3＇-methyl-debranone-like molecule）） already described, and a new one （AR36）, for which the synthesis is presented, are biologically compared with the well-known GR24 and the recently identified CISA-1. These different structures emphasize the wide range of parts attached to the D-ring for the bioactivity as a plant hormone. These new compounds possess a common dimethylbutenolide motif but their structure varies in the ABC part of the molecules： 23 has the same ABC part as GR24, while 31 and AR36 carry, respectively, an aromatic ring and an acyclic carbon chain. Detailed information is given for the bioactivity of such derivatives in strigolactone synthesis or in perception mutant plants （pea rmsl and rms4, Arabidopsis max2 and, max4） for different hormonal functions along with their action in the rhizosphere on arbuscular mycorrhizal hyphal growth and parasitic weed germination.

关键词： strigolactones synthetic analogs Pisum sativum Arabidopsis root parasitic plants Gigaspora rosea plant growth regulator.

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A Phelipanche ramosa KAI2 protein perceives strigolactones and isothiocyanates enzymatically

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Plant Communications 2021年第5期2卷 132-147页

作者： Alexandre de Saint Germain Anse Jacobs Guillaume Brun jean-bernard pouvreau Lukas Braem David Cornu Guillaume Clavé Emmanuelle Baudu Vincent Steinmetz Vincent Servajean Susann Wicke Kris Gevaert Philippe Simier Sofie Goormachtig Philippe Delavault François-Didier Boyer Institut jean-Pierre Bourgin INRAEAgroParisTechUniversitéParis-Saclay78000 VersaillesFrance Department of Plant Biotechnology and Bioinformatics Ghent University9052 GentBelgium Center for Plant Systems Biology VIB9052 GentBelgium Department of Biomolecular Medicine Ghent University9000 GhentBelgium Center for Medical Biotechnology VIB9000 GhentBelgium Laboratoire de Biologie et Pathologie Végétales(LBPV) Equipe d’Accueil 1157Universitéde Nantes44000 NantesFrance Institute for Biology Humboldt-Universit€at zu Berlin10115 BerlinGermany UniversitéParis-Saclay CEACNRSInstitute for Integrative Biology of the Cell(I2BC)91198Gif-sur-YvetteFrance UniversitéParis-Saclay CNRSInstitut de Chimie des Substances NaturellesUPR 230191198Gif-sur-YvetteFrance

Phelipanche ramosa is an obligate root-parasitic weed that threatens major crops in central *** order to germinate,it must perceive various structurally divergent host-exuded signals,including isothiocyanates(ITCs)and strigolactones(SLs).However,the receptors involved are still ***,we identify five putative SL receptors in *** and show that PrKAI2d3 is involved in the stimulation of seed *** demonstrate the high plasticity of PrKAI2d3,which allows it to interact with different chemicals,including *** SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic *** provide evidence that PrKAI2d3 enzymatic activity confers hypersensitivity to ***,we demonstrate that methylbutenolide-OH binds PrKAI2d3 and stimulates *** germination with bioactivity comparable to that of *** study demonstrates that *** has extended its signal perception system during evolution,a fact that should be considered for the development of specific and efficient biocontrol methods.

关键词： a/b-hydrolase receptor seed germination stimulant strigolactones isothiocyanates Phelipanche ramosa

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Density Results in Sobolev Spaces Whose Elements Vanish on a Part of the Boundary

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Chinese Annals of Mathematics,Series B 2011年第6期32卷 823-846页

作者： jean-Marie Emmanuel bernard Laboratoire Analyse et Probabitilités Université d'Evry val d'Essonne23 Boulevard de France91037 EVRYFrance

This paper is devoted to the study of the subspace of Wm＇＂ of functions that vanish on a part γ0 of the boundary. The author gives a crucial estimate of the Poincare constant in balls centered on the boundary of γ0. Then, the convolution-translation method, a variant of the standard mollifier technique, can be used to prove the density of smooth functions that vanish in a neighborhood of γ0, in this subspace. The result is first proved for m = 1, then generalized to the case where m 〉 1, in any dimension, in the framework of Lipschitz-continuous domain. However, as may be expected, it is needed to make additional assumptions on the boundary of γ0, namely that it is locally the graph of some Lipschitz-continuous function.

关键词： Density results Boundary value problems Sobolev spaces

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Combination of Tsoft and ET34-ANA-V80 software for the preprocessing and analysis of tide gauge data in French Polynesia

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Geodesy and Geodynamics 2023年第1期14卷 26-34页

作者： bernard Ducarme jean-Pierre Barriot Fangzhao Zhang Catholic University of Louvain Georges Lemaître Centre for Earth and Climate Research(ELI)1348 Louvain-la-NeuveBelgium Geodesy Observatory of Tahiti University of French Polynesia(UPF)PunaauiaFrench Polynesia College of Geodesy and Geomatics Shandong University of Science and TechnologyQingdao 266590China

Since 2008 a network of five sea-level monitoring stations was progressively installed in French *** stations are autonomous and data,collected at a sampling rate of 1 or 2 min,are not only recorded locally,but also transferred in real time by a radio-link to the NOAA through the GOES *** new ET34-ANA-V80 version of ETERNA,initially developed for Earth Tides analysis,is now able to analyze ocean tides *** a two-step validation scheme,we took advantage of the flexibility of this new version,operated in conjunction with the preprocessing facilities of the Tsoft software,to recover co rrected data series able to model sea-level variations after elimination of the ocean tides *** performed the tidal analysis of the tide gauge data with the highest possible selectivity(optimal wave grouping)and a maximum of additional terms(shallow water constituents).Our goal was to provide corrected data series and modelled ocean tides signal to compute tide-free sea-level variations as well as tidal prediction models with centimeter *** also present in this study the characteristics of the ocean tides in French Polynesia and preliminary results concerning the non-tidal variations of the sea level concerning the tide gauge setting.

关键词： Tide gauges Tidal data processing Mean sea level

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Conductance hysteresis and inelastic excitations at hydrogen decorated cerium atoms and clusters in a tunnel junction

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Nano Research 2016年第10期9卷 3171-3178页

作者： Rouzhaji Tuerhong Shawulienu Kezilebieke bernard Barbara jean-Pierre Bucher Institut Universitaire de France Universite de Strasbourg 23 rue du Loess F-67034 Strasbourg France Institut Neel CNRS Universite Grenoble-Alpes F-38042 Grenoble France

Voltage-controlled conductance and switching induced by single molecules or atoms are ideally studied in scanning tunneling microscope （STM） tunnel junctions. While the objects under consideration are mostly used in their original form, little is known of the possibilities of in situ adjustments of their properties. Here, we evidence properties of a tunnel junction made of a Ce atom/cluster built by atomic manipulation on Au（111） at a temperature of 4.6 K in the presence of H2. The conductance through the object is characterized by a switching voltage corresponding to an opening or closing of an inelastic electron tunneling conductance channel at 50 mV for a Ce atom and 140 mV for a Ce cluster and by charging. We demonstrate that the electronic properties of an STM junction can be engineered in a simple way by in situ guiding of the H2 pinning at an atomic cluster.

关键词： conductance hysteresis inelastic electron tunelingspectroscopy (IETS) switching Ce clusters scanning tunneling microscope (STM)/scanning tunneling spectroscopy (STS)

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Monitoring absolute vertical land motions and absolute sea-level changes from GPS and tide gauges data over French Polynesia

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Geodesy and Geodynamics 2024年第1期15卷 13-26页

作者： Xianjie Li jean-Pierre Barriot bernard Ducarme Marania Hopuare Yidong Lou Geodesy Observatory of Tahiti University of French PolynesiaFaa'a 98702French Polynesia Catholic University of Louvain Georges Lemaître Centre for Earth and Climate Research(ELI)1348 Louvain-la-NeuveBelgium GNSS Research Center Wuhan UniversityWuhan 430079China State Key Laboratory of Information Engineering in Surveying Mapping and Remote SensingWuhan UniversityWuhan 430079China

In this study,we estimate the absolute vertical land motions at three tidal stations with collocated Global Navigation Satellite System(GNSS)receivers over French Polynesia during the period 2007-2020,and obtain,as ancillary results,estimates of the absolute changes in sea level at the same *** verify our processing approach to determining vertical motion,we first modeled vertical motion at the International GNSS Service(IGS)THTI station located in the capital island of Tahiti and compared our estimate with previous independent determinations,with a good *** obtained the following estimates for the vertical land motions at the tide gauges:Tubuai island,Austral Archipelago-0.92±0.17 mm/yr,Vairao village,Tahiti Iti:-0.49±0.39 mm/yr,Rikitea,Gambier Archipelago-0.43±0.17 mm/*** absolute variations of the sea level are:Tubuai island,Austral Archipelago 5.25±0.60 mm/yr,Vairao village,Tahiti Iti:3.62±0.52 mm/yr,Rikitea,Gambier Archipelago 1.52±0.23 mm/*** discuss these absolute values in light of the values obtained from altimetric measurements and other means in French Polynesia.

关键词： GPS Tide gauges Sea level changes Vertical land motion

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Enhancing laccase stability and activity for dyes decolorization using ZIF-8@MWCNT nanocomposite

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Chinese Journal of Chemical Engineering 2022年第8期35卷 66-75页

作者： Pascal Habimana Yanjun Jiang Jing Gao jean bernard Ndayambaje Osama M.Darwesh jean Pierre Mwizerwa Xiaobing Zheng Li Ma School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China University of Rwanda College of Science and Technology3900 KigaliRwanda Department of Agricultural Microbiology National Research CentreCairo 12622Egypt College of Mechatronics and Control Engineering Shenzhen UniversityShenzhen 518060China

The continuous use of chemical dyes in various industries,and their discharge into industrial effluents,results in severe problems to human life and water *** have the ability to decolorize dyes and toxic chemicals in industrial effluents as green *** possible industrial applications have been limited by poor reusability,low stability,and loss of free laccase *** this research,lac-case was immobilized on zeolitic imidazolate framework coated multi-walled carbon nanotubes(Laccase@ZIF-8@MWCNTs)via metal affinity adsorption to develop an easy separable and stable *** optimum reaction conditions for immobilized laccase are at a pH of 3.0 and a temperature of 60℃.The immobilized laccase was enhanced in storage and thermal *** results indicated that Laccase@ZIF-8@MWCNTs still maintained 68%of its original activity after 10 times of repeated *** importantly,the biocatalytic system was applied for decolorization of different dyes(20 mg·L^(-1))without a mediator,and up to 97.4%for Eriochrome black T and 95.6%Acid red 88 was achieved in 25 *** with these properties may be used in a variety of environmental and industrial applications.

关键词： Metal-organic framework Zeolitic imidazolate framework Reusability and stability Immobilized laccase Dye decolorization

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Efficacy and safety profile of LCR35 complete freeze-dried culture in irritable bowel syndrome:A randomized,double-blind study

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World Journal of Gastroenterology 2012年第17期18卷 2067-2075页

作者： Michel Dapoigny Thierry Piche Philippe Ducrotte bernard Lunaud jean-Michel Cardot Annick Bernalier-Donadille Médecine Digestive Centre Hospitalier Universitaire(CHU) Estaing CHU Clermont-FerrandClermont UniversitéInserm UMR 76663001 F-Clermont-FerrandFrance Department of Gastroenterology and Inserm 576 Hpital de l'Archet 206202 NiceCedex 3France Department of Hepatogastroenterology and Nutrition Hpital Charles NicolleInserm UMR 107376031 RouenCedexFrance Department of Gastroenterology Centre République63000 Clermont-FerrandFrance Biopharmacy Department Faculty of Pharmacy63000 Clermont-FerrandFrance UR454 Microbiology Division Institut National de la Recherche AgronomiqueResearch Centre of Clermont-Ferrand-Theix63122 Saint Genès-ChampanelleFrance

AIM:To assess the effects and safety of Lactobacillus casei rhamnosus LCR35 complete freeze-dried culture(LCR35) in patients suffering from irritable bowel syndrome(IBS).METHODS:A randomized,double-blind pilot study was performed in 50 patients complaining of IBS symptoms complying with RomeⅢ*** were allocated to receive either LCR35(n = 25) at a minimum daily dose of 6 × 108 colony forming units or placebo(n = 25) for 4 *** inclusion,after treatment and 2 wk later,patients completed the IBS severity *** from baseline in the IBS severity score at the end of treatment was the primary efficacy *** were compared between groups in the whole population and in IBS subtypes(IBS with predominance of constipation,IBS with predominance of diarrhoea,mixed IBS,unsubtyped IBS).The presence of lactobacillus casei rhamnosus in stools was investigated at inclusion and at the end of *** gastrointestinal quality of life questionnaire and the hospital anxiety and depression(HAD) scale were also ***:Both groups were balanced for baseline *** 85% of patients,stool analyses showed that lactobacillus casei rhamnosus able to survive in the digestive *** the whole population,improvements in the IBS severity score did not differ significantly between treatments with a 25% decrease after 4-wk treatment,and a 15% decrease from baseline 2 wk later in both *** IBS subgroups,statistical analysis could not be performed due to small sample size,but a clinical response in favour of LCR35 was observed in IBS patients with predominance of diarrhoea:no change in the symptom severity score was seen with the placebo after 4 wk treatment,whereas a clinically relevant decrease occurred with LCR35(-37% vs-3%).Furthermore,in spite of an increase in symptom intensity,the IBS severity score was maintained below the baseline value 2 wk later with LCR35(-19% from baseline),whilst a slight 5% increase from baseline was observ

关键词： Irritable bowel syndrome Lactobacillus casei rhamnosus Probiotics Symptom severity score

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Cambrian explosion fossils from the North China craton

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National Science Review 2022年第7期9卷 13-13页

作者： jean-bernard Caron Department of Natural History Royal Ontario Museum

Our understanding of the Cambrian explosion has relied heavily on a few exceptional fossil deposits preserving soft-bodied animals,the so-called Burgess-Shale-type deposits,or BSTs for *** are named after the famous Canadian fossil deposit discovered by Charles Walcott in *** ofwhere they come from or their age,all BSTs preserve soft-bodied fossils as carbonaceous remains.

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Management of digestive bleeding related to portal hypertension in cirrhotic patients:A French multicenter cross-sectional practice survey

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World Journal of Gastroenterology 2006年第48期12卷 7810-7814页

作者： Pierre Ingrand Jérme Gournay Pierre bernard Frédéric Oberti Brigitte bernard-Chabert Arnault Pauwels Philippe Renard Eric Bartoli jean-Franois Cadranel jean-Claude Barbare Isabelle Ingrand Michel Beauchant Clinical Research Center Poitiers University France Hepato-Gastroenterology Centre Hospitalier Universitaire Nantes France Hepato-Gastroenterology Centre Hospitalier Universitaire Saint-André Bordeaux France Hepato-Gastroenterology Centre Hospitalier Universitaire Angers France Hepato-Gastroenterology Centre Hospitalier Universitaire Reims France Hepato-Gastroenterology Centre Hospitalier Gonesse France Hepato-Gastroenterology Centre Hospitalier Argenteuil France Hepato-Gastroenterology Centre Hospitalier Universitaire Amiens France Hepato-Gastroenterology Centre Hospitalier Creil France Hepato-Gastroenterology Centre Hospitalier Compiègne France Hepato-Gastroenterology Centre Hospitalier Universitaire Poitiers France

AIM: To investigate the conformity of management practices of gastrointestinal hemorrhage in cirrhotic patients with relevant guidelines. METHODS: A questionnaire on the management of digestive bleeding was completed for all consecutive cirrhotic patients admitted to 31 French hospitals. RESULTS: One hundred and twenty-six bleeding events were recorded. It was the first bleeding episode in 79 patients (63%), of whom 40 (51%) had a prior diagnosis of cirrhosis and 25 (32%) had previously undergone an endoscopy. The bleeding episode was a recurrence in 46 patients (37%). The median time between onset and admission was 4 h, but exceeded 12 h in 42% of cases. There was an agreement between centers forearly vasoactive drug administration (87% of cases), association with ligation (42%) more often than sclerosis (21%) at initial endoscopy, and antibiotic prophylaxis (64%). By contrast, prescription of beta-blockade alone or in combination (0 to 100%, P = 0.003) for secondary prophylaxis and lactulose (26% to 86%, P = 0.04), differed among centers. CONCLUSION: In French hospitals, management of bleeding related to portal hypertension in cirrhotic patients is generally in keeping with the consensus. Broad variability still remains concerning beta-blockade use for secondary prophylaxis. Screening for esophageal varices, the use of antibiotic prophylaxis and patients information need to be improved.

关键词： Digestive bleeding Portal hypertension Cirrhosis Evaluation studies

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