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Plasticity of wheat seedling responses to K^(+) deficiency highlighted by integrated phenotyping of roots and root hairs over the whole root system

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Stress Biology 2023年第1期3卷 69-83页

作者： Ikram Madani jean-benoit peltier Martin Boeglin Hervé Sentenac Anne-Aliénor Véry Institut des Sciences des Plantes de Montpellier UMR 5004 CNRS-386 INRAE-Université Montpellier-Institut AgroCampus SupAgro-INRAE Bat 7Place VialaMontpellier 34060 Cedex 2France

The availability in the soil of potassium(K^(+)),a poorly mobile macronutrient required in large quantities for plant growth,is generally suboptimal for crop production in the absence of fertilization,making improvement of the ability of crops to adapt to K^(+)deficiency stress a major *** the uptake capacity of the root system is among the main strategies to achieve this ***,we report an integrative approach to examine the effect of K^(+)deficiency on the development of young plant entire root system,including root hairs which are known to provide a significant contribution to the uptake of poorly mobile nutrients such as K^(+),in two genetically distant wheat varieties.A rhizobox-type methodology was developed to obtain highly-resolved images of root and root hairs,allowing to describe global root and root hair traits over the whole root system via image analysis *** two wheat varieties responded differently to the K^(+)shortage:Escandia,a wheat ancestor,reduced shoot biomass in condition of K^(+)shortage and substantially increased the surface area of its root system,specifically by increasing the total root hair *** Zenati,a landrace,conversely appeared unresponsive to the K^(+)shortage but was shown to constitutively express,independently of the external K^(+)availability,favorable traits to cope with reduced K^(+)availability,among which a high total root hair ***,valuable information on root system adaptation to K^(+)deficiency was provided by global analyses including root hairs,which should also be relevant for other nutrient stresses.

关键词： K^(+)deficiency Root hair Root system Phenotyping Wheat Rhizobox Root plasticity

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Matrix Assisted Laser Desorption Ionization Time of Flight(MALDI-TOF)-Mass Spectrometry and^(13)C-NMR-Identified New Compounds in Paraberlinia bifoliolata(Ekop-Beli)Bark Tannins

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Journal of Renewable Materials 2024年第3期12卷 553-568页

作者： Liliane Nga benoit Ndiwe Achille Bernard Biwolé Antonio Pizzi jean Jalin Eyinga Biwole Joseph Zobo Mfomo Laboratory of Forest Resources and Wood Valorization Training Unit in Engineering SciencesPost Graduate School of Fundamental and Applied SciencesUniversity of DoualaP.O.Box 1872DoualaCameroun Laboratory of Mechanics Training Unit in Engineering SciencesPost Graduate School of Fundamental and Applied SciencesUniversity of DoualaP.O.Box 2701DoualaCameroun Laboratory of Studies and Research on Wood Material(LERMAB) University of LorraineNancyFrance

Extracts of plant origin,particularly tannins,are attracting growing interest for the sustainable development of materials in the industrial *** discovery of new tannins is therefore *** aim of this work was to contribute to the understanding of the properties of Paraberlinia bifoliolata tannin by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectroscopy MALDI-TOF/MS and Carbon 13 Nuclear Magnetic Resonance(13C NMR).The chemical composition of tannin extracted from Paraberlinia bifoliolata bark was determined,as was the mechanical strength of the resin hardened with Acacia nilotica *** by successive water extraction was 35%.MALDI-TOF/MS analysis revealed the presence of three new compounds in this tannin,previously unknown in this family of *** are 3-hydroxyproline acid,N-methyl-4-hydroxypipecolic acid and N-methyl-5-dihydroxypipecolic *** identification of the above molecules means that this tannin can be used for industrial applications,as a resin in the manufacture of particleboard and in the formulation of green corrosion *** information is reinforced by 13C NMR spectrometry,which indicates the presence of several polyflavonoid units,confirming the condensed nature of the *** analysis of the resin formed by the purified tannin of Paraberlinia bifoliolata to which a vegetal biohardener has been added provided a Modulus of Elasticity(MOE)value of 4840 MPa at 150℃,confirming its possible use as a binder resin in the manufacture of wood panels as well as for the formulation of a corrosion inhibitor.

关键词： Tannin Paraberlinia bifoliolata Central African wood species MALDI-TOF/MS 13C NMR bark extracts thermomechanical behaviour wood-binder

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Using Optical Tweezers to Study the Friction of the Red Blood Cells

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Advances in Bioscience and Biotechnology 2024年第2期15卷 100-111页

作者： Edoukoua jean Michel Konin Pavel Yale Abadê Ange-Boris N’guessan Kouassi benoit Kouakou Abaka Michel Kouacou Eugene Megnassan Département des Sciences et Techniques Université Alassane Ouattara Bouaké C&#244te d’Ivoire Laboratoire d’Instrumentation Image et Spectroscopie (L2IS) Institut National Polytechnique Félix Houphou&#235t-Boigny (INPHB) Yamoussoukro C&#244te d’Ivoire Classes préparatoires aux grandes Ecoles Université de San Pedro San Pedro C&#244te d’Ivoire Laboratoire de Physique Fondamentale et Appliquée Université Nangui Abrogoua (UNA) Abidjan C&#244te d’Ivoire

In the last two decades the study of red blood cell elasticity using optical tweezers has known a rise appearing in the scientific research with regard to the various works carried out. Despite the various work done, no study has been done so far to study the influence of friction on the red blood cell indentation response using optical tweezers. In this study, we have developed a new approach to determine the coefficient of friction as well as the frictional forces of the red blood cell. This approach therefore allowed us to simultaneously carry out the indentation and traction test, which allowed us to extract the interfacial properties of the microbead red blood cell couple, among other things, the friction coefficient. This property would be extremely important to investigate the survival and mechanical features of cells, which will be of great physiological and pathological significance. But taking into account the hypothesis of friction as defined by the isotropic Coulomb law. The experiment performed for this purpose is the Brinell Hardness Test (DB).

关键词： Friction Indentation Optical Tweezers Tribology Red Blood Cells

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Supervised Learning Method: Critical Analysis and Updating of Cubing Rate Formulas for Determining Bark Masses of Prunus africana (Hook. f.) Kalkman (Rosaceae) in Cameroon

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Open Journal of Forestry 2024年第3期14卷 346-368页

作者： Armand Ndedy Bile Alain Mpouam benoit Fouadjio Lorette Guedeu Kambale Muhesi Steve Tassiamba Paulette Kouam Eric Wete Marius Ela Michèle Yimga Joseph Ambara Louis Aimé Fono jean Lagarde Betti Department of Plant Biology Faculty of Science The University of Douala Douala Cameroon Department of Wood and Forest Sciences ISABEE University of Bertoua Bertoua Cameroon Department of Water and Forests Higher Institute of Agronomic Veterinary and Forestry Studies of Butembo Butembo Democratic Republic of Congo Department of Forestry Faculty of Agronomy and Agricultural Sciences University of Dschang Dschang Cameroon Department of Biology and Plant Faculty of Sciences University of Maroua Maroua Cameroon Deutsche Gresellschaft fur Internationale Zusammenarbeit GmbH (GIZ) Bertoua Cameroon Department of Mathematics and Computer Science Faculty of Science The University of Douala Douala Cameroon

The stem barks of Prunus africana are used in the treatment of the benign prostate. Cameroon is one of the important exporters of the barks. Despite the important measures adopted in Cameroon for sustaining its harvesting, some many chalenges still remain. The objective of this work is to refine the forest management parameters in relation to P. africana in the regions of Adamaoua and the South-West by developing a volume rate which makes it possible to estimate the production for a new stem. The work took place in two phases: in the South-West in 2010 and in Adamaoua in 2011. Data collection used the semi-direct method, while the cubing equation was deduced by the multiple linear regression method. Two models for volume estimation and three models for mass prediction were developed. The predictive parameters retained are diameter, height of the bole and thickness of the bark. Results show that the average mass of the dry bark for a given P. africana tree species is 27.55 ± 14.44 kg and this varies according to the site. The strong adjusted coefficient of determination (adjusted R2) observed illustrates the reliability of the proposed models. These models provide a reliable tool that can be adopted as a standard in Cameroon for P. africana.

关键词： Prunus africana Prostate Quota Mass Model Sustainable Management

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Efficacy and tolerability of chitin-glucan combined with simethicone(GASTRAP^(■)DIRECT)in irritable bowel syndrome:A prospective,open-label,multicenter study

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World Journal of Gastrointestinal Pharmacology and Therapeutics 2024年第3期15卷 10-22页

作者： Nathalie Talbodec Pauline Le Roy Peggy Fournier benoit Lesage Elodie Lepoutre François Castex jean Michel Godchaux Lionel Vandeville Benjamin Bismuth Xavier Lesage Pauline Bayart Michael Genin Christel Rousseaux Veronique Maquet Salvatore Modica Pierre Desreumaux Caroline Valibouze Department of Gastroenterology Hôpital privéLe BoisLille 59000France Department of Gastroenterology Hôpital privéde Villeneuve d’AscqVilleneuve d’Ascq 59650France Univ.Lille CHU LilleULR 2694–METRICSÉvaluation des Technologies de Santéet des Pratiques MédicalesLille 59000France Development of Intestinal Biotech 1 Avenue Oscar LambretLille 59045France KitoZyme SA Parc Industriel des hauts Sarts Zone 2Rue de MilmortHerstal 4040Belgium BiOkuris A Parc Industriel des hauts Sarts Zone 2Rue de MilmortHerstal 4040Belgium Department of Hepato-Gastroenterology Lille University HospitalLille 59000France U1286-INFINITE Institute for Translational Research in InflammationUniv.LilleInsermCHU LilleLille 59000France Department of Digestive Surgery and Transplantation Lille University HospitalLille 59037France

BACKGROUND Irritable bowel syndrome(IBS),defined according to the Rome IV diagnostic criteria,is a chronic functional gastrointestinal disorder characterized by recurrent abdominal pain related to altered bowel ***-line recommended treatments are limited to combining drugs targeting predominant symptoms,particularly pain(antispasmodics),constipation(laxatives),and diarrhea(loperamide),yielding only a limited therapeutic ***^(■)DIRECT is a class IIa medical formulation composed of a combination of chitin-glucan and simethicone indicated for the symptomatic treatment of gas-related gastrointestinal disorders by combining different mechanisms of *** To evaluate the efficacy,tolerability,and safety of 4-week GASTRAP^(■)DIRECT treatment in patients with *** In this prospective,multicenter,open-label trial,120 patients with IBS received three sticks of GASTRAP^(■)DIRECT(1.5 g/d of chitin-glucan and 0.75 mg/d of simethicone)per day for 4 *** primary endpoint was the responder rate,defined as the number of patients whose abdominal pain score decreased by≥30%from baseline to week(W)*** analysis was performed using the per-protocol *** symptoms,impact of global symptoms on daily life,change in stool consistency,and improvement in defecatory disorders were *** Overall,100 patients were *** W4,67%(95%CI:57-75)showed improvement in abdominal pain(score:5.8±2.4 vs 2.9±2.0,P<0.0001).Similar improvements were observed for bloating[8.0±1.7 vs 4.7±2.9,P<0.0001;60%(95%CI:50-70)responders],abdominal distension[7.2±2.1 vs 4.4±3.1,P<0.0001;53%(95%CI:43-63)responders],and impact of global symptoms on daily life[7.1±2.0 vs 4.6±2.9,P<0.0001;54%(95%CI:44-64)responders].Stool consistency improved in most patients(90%and 57%for patients with liquid and hard stools,respectively).Overall,42%of patients with defecatory disorders reported very much/considerable improvements by *** severe adverse event occurred,and tolerabil

关键词： Chitin-glucan Irritable bowel syndrome Abdominal pain Flatulence Defecatory disorders Stool consistency Natural non-pharmacological treatment

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Analysis of a System Describing Proliferative-Quiescent Cell Dynamics

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Chinese Annals of Mathematics,Series B 2018年第2期39卷 345-356页

作者： jean CLAIRAMBAULT benoit PERTHAME Andrada Quillas MARAN Dedicated to Philippe G. Ciarlet on the occasion of his 80th birthda

Systems describing the dynamics of proliferative and quiescent cells are commonly used as computational models, for instance for tumor growth and *** on the very earliest stages of hematopoiesis, stem cells and early progenitors, the authors introduce a new method, based on an energy/Lyapunov functional to analyze the long time behavior of solutions. Compared to existing works, the method in this paper has the advantage that it can be extended to more complex situations. The authors treat a system with space variable and diffusion, and then adapt the energy functional to models with three equations.

关键词： Stability analysis Lyapunov functional Energy method Parabolic sys-tems Proliferative and quiescent cells Tumor growth Hematopoiesis

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Influence of initial microstructure and grain size on transformation of bainite to austenite in large size forgings

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Journal of Iron and Steel Research International 2018年第5期25卷 554-562页

作者： Emna Ben Fredj Hadi Ghasemi Nanesa Mohammad Jahazi jean-benoit Morin Department of Mechanical Engineering Ecole deTechnologie Superieure Montreal QC H3C 1K3 Canada Finkl Steel-Sorel St-Joseph-de-Sorel QC J3R 3M8 Canada

The kinetics of austenite formation in the surface and center regions of a 40 t forged ingot of a high-strength medium- carbon low-alloy steel was studied using high-resolution dilatometry. The starting microstructures from the surface or center regions had different proportions of bainite and residual austenite as well as different prior austenite grain sizes. Two heating rates representing the actual heating rates in the surface （5℃ s -1） and center regions （0.5℃ s -1） of large size forged blocks were utilized. Dilatometric curves revealed only one transformation step of austenite formation at both heating rates independent of grain size or proportion of phases. Optical microscopy, field emission gun scanning electron microscopy and X-ray diffraction were used to study microstructure evolution and confirm the results obtained by dilatometry. The kinetic parameters for austenite formation were determined from the dilatometry data by Johnson-Mehl- Avrami-Kolmogorov （JMAK） equation. The JMAK coefficients were determined for each condition of the investigated steels. The calculations indicated that the nucleation and growth of austenite in the surface region were accelerated more than 10,000 times due to a significantly smaller average prior austenite grain size, stability of initial retained austenite, and accumulation of coarse carbides at the surface. The results were discussed in the framework of classical nucleation and growth theories using the kinetic parameters for austenite formation.

关键词： Large size ingot Medium-carbon low-alloy steel Initial microstructure Grain size Austenite formation

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Autoregressive neural networks with exogenous variables for indoor temperature prediction in buildings

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Building Simulation 2021年第1期14卷 165-178页

作者： benoit Delcroix Jérôme Le Ny Michel Bernier Muhammad Azam Bingrui Qu jean-Simon Venne Department of Mechanical Engineering Polytechnique Montréal2500 Chemin de PolytechniqueMontréalQCH3T 1J4Canada Department of Electrical Engineering and GERAD Polytechnique Montréal2500 Chemin de PolytechniqueMontréalQCH3T 1J4Canada Smart Building Lab BrainBox AIMontréalQCCanada

Thermal models of buildings are helpful to forecast their energy use and to enhance the control of their mechanical ***,these models are building-specific and require a tedious,error-prone and time-consuming development effort relying on skilled building energy *** to white-box and gray-box models,data-driven(black-box)models require less development time and a minimal amount of information about the building *** this paper,autoregressive neural network models are compared to gray-box and black-box linear models to simulate indoor *** models are trained,validated and compared to actual experimental data obtained for an existing commercial building in Montreal(QC,Canada)equipped with roof top units for air *** show that neural networks mimic more accurately the thermal behavior of the building when limited information is available,compared to gray-box and black-box linear *** gray-box model does not perform adequately due to its under-parameterized nature,while the linear models cannot capture non-linear phenomena such as radiative heat transfer and ***,the neural network models outperform the alternative models in the presented application,reaching a coefficient of determination R2 up to 0.824 and a root mean square error down to 1.11℃,including the error propagation over time for a 1-week period with a 5-minute *** considering a 50-hour time horizon,the best neural networks reach a much lower root mean square error of around 0.6℃,which is suitable for applications such as model predictive control.

关键词： thermal model building simulation experimental validation multi-layer perceptron linear vs.non-linear black-box vs.gray-box

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Assessment of left-ventricular diastolic function in pediatric intensive-care patients:a review of parameters and indications compared with those for adults

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World Journal of Pediatrics 2021年第1期17卷 21-30页

作者： Morgan Recher Astrid Botte Jerome Soquet jean-benoit Baudelet François Godart Stephane Leteurtre ULR 2694-METRICS:évaluation des Technologies de santéet des Pratiques Médicales Univ.LilleCHU Lille59000 LilleFrance Réanimation et Surveillance Continue Pédiatriques Hopital Jeanne de FlandreCHU de Lille59037 Lille CedexFrance Pediatric and Neonatal Intensive Care Unit CHU Bordeaux33076 BordeauxFrance Cardiac Surgery Unit CHU Lille59000 LilleFrance Congenital and Pediatric Cardiology Unit CHU Lille59000 LilleFrance

Background The incidence of diastolic heart failure has increased over *** evaluation of left-ventricular diastolic func-tion is complex,ongoing,and remains poorly performed in pediatric intensive-care *** study aimed to review the literature and to provide an update on the evaluation of left-ventricular diastolic function in adults and children in intensive *** sources We searched data from PubMed/***-two studies were *** pragmatic questions were identified:(1)What is the physiopathology of diastolic dysfunction?(2)Which tools are required to evaluate diastolic func-tion?(3)What are the echocardiographic criteria needed to evaluate diastolic function?(4)When should diastolic function be evaluated in pediatric intensive care?Results Early diastole allows characterization of relaxation,whereas compliance assessments and filling pressures are evalu-ated during late *** evolution of diastolic function differs between adults and *** in adults,decreased compliance occurs at the same time as delayed relaxation in *** function can be evaluated by Doppler *** echocardiographic criteria for ventricular relaxation include the E wave,E/A wave ratio,and isovolumic relaxation *** compliance can be assessed by the E/e'wave ratio,atrial volume,and Ap wave duration during pulmonary vein *** adult intensive-care patients,the E/e'ratio can be used as an index of tolerance for volume expan-sion in septic patients and to adjust the inotropic *** Clinical studies would allow some of these parameters to be validated for use in children in intensive care.

关键词： Diastole Echocardiography Heart failure Intensive-care unit Pediatric

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Prevalence and Factors Associated with Sharing of Human Immunodeficiency Seropositivity Status among Serodiscordant Couples in the City of Parakou (Benin)

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Open Journal of Epidemiology 2024年第1期14卷 1-18页

作者： Djossou Sègnon Eurydice Elvire Damien Georgia Barikissou Meba Bio Mamatou Tokpanoude Ignace Ahanhanzo-Glele Rhonel Ataigba Iretinethania Elie Agossoukpe benoit Kakpo-Zannou Rodrigue Kamoutchoni Corneille Aguemon Badirou Unité de santé publique de la Faculté des Sciences de la santé (FSS) Universitéd’Abomey-Calavi (UAC) Cotonou Bénin Association des Médecins Communautaires de Bénin (AMCB) Parakou Bénin Association Béninoise pour la Promotion de la Famille (ABPF) Parakou Bénin Centre d’Information de Prospective d’Ecoute et de Conseil sur les IST/VIH/Sida du Borgou et de l’Alibori (CIPEC/B-A) Parakou Bénin Institut de Formation enSoinsInfirmiers et Obstétricaux (IFSIO) Faculté de Médecine (FMP) Parakou Bénin Service de Psychiatrie Centre HospitalierUniversitaireDépartemental du BorgouAlibori (CHUD/B-A) Parakou Bénin Service de Médecine Interne H&#244pital Saint-Jean de Dieu de Boko N’Dali Bénin

Introduction: In serodiscordant couples, sharing serostatus is a crucial step in the HIV risk management process. The aim of this study was to determine the prevalence and factors associated with serostatus sharing among HIV serodiscordant couples in the city of Parakou. Setting and methods: This study was carried out in the city of Parakou, capital of northern Benin. It was a descriptive, analytical cross-sectional study with non-probability sampling for convenience. Socio-demographic data, as well as data on antecedents, behaviours, social and marital life, were collected using a questionnaire gave to the participants. Clinical and therapeutic data were collected from patients’ medical records and follow-up charts. Data were analyzed and processed using R 4.3.1 software. The significance level was set at 5%, and logistic regression was used to identify potential predictors of shared serostatus among serodiscordant couples in the city of Parakou in 2022. Results: A total of 299 participants in long-term serodiscordant relationships were included in our study. The average age of the subjects was 37.55 ± 10.25 years. Women were the infected partner in 80.27% of cases. The most common level of education was secondary (33.45%). Most respondents (230 subjects, 76.92%) were married. The average duration of the couple’s relationship was 121.68 months ± 96.32. The most frequent screening circumstances were: Prevention of mother-to-child transmission (PMTCT) (50.84%) and hospital screening (26.09%). The infected partner shared his or her serostatus with his or her spouse in 47.83% of cases. Potential predictors of serostatus sharing within the couple were: marital status (p Conclusion: The sharing of serostatus within serodiscordant couples needs to be managed in a way that takes into account the social and marital characteristics of the couple.

关键词： Serodiscordance Sharing of Serostatus HIV Partner Benin

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