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First report of Phytopythium vexans causing the“Avocado sadness”in Michoacan,Mexico

Phyton-International Journal of Experimental Botany 2019年第1期88卷 11-13页

作者： Hernandez Perez A E Cerna Chavez jc.de.gado ortiz M Beltran Beache LM Tapia Vargas YM Ochoa Fuentes Estudiante del postgrado Universidad Autonoma Agraria Antonio Narro Departamento de ParasitologiaBuenavistaSaltilloCoahuilaMexico CP 25315 Universidad Autonoma Agraria Antonio Narro Departamento de ParasitologiaBuenavistaSaltilloCoahuilaMexico CP 25315 Catedratico CONACYT-UAAAN.Universidad Autonoma Agraria Antonio Narro Departamento de ParasitologiaBuenavistaSaltilloCoahuilaMexico CP 25315 CULTA S.A.de C.V. InvestigacionDesarrolloAltavistaEl ManteTamaulipasMexico CP 898800 INIFAP Campo Experimental Uruapan.Av.Latinoamericana No.1101Col.Revolucion CP.60500UruapanMichoacanMexico

Mexico is the main producer,consumer and exporter of avocado in the world,being Michoacan the main producer state contributing more than 80%of the national *** are phytopathogens that decimate the production causing the death of the *** samples were collected in avocado trees that showed the characteristic symptomatology of the disease known as avocado sadness,the sampling was carried out in four of the main avocado producing towns,in the state of Michoacan,*** isolation consisted in sowing root tissue in Petri dishes with V8R-PARPH culture medium,subsequently they were identified morphologically and for species level it was determined by molecular biology,with the PCR-ITS *** tests were performed in triplicate with avocado seedlings with more than six *** 24 hours,the inoculated plants expressed decay in the apical part,after 120 hours the leaves showed yellowing and after 15 days there was a generalized wilt on the stem and leaves,re-isolating the phytopathogen Phytopythium *** study confirms the first report of the oomycete *** affecting avocado trees in the most important producing region of the Mexican Republic.

关键词： Molecular identification Pathogenicity Phytopythium vexans Avocado.

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Pontocerebellar Progressive Multifocal Leukoencephalopathy. Radiological, Clinical, Histological and Immunohistochemical Findings in a Hematological Patient

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Open Journal of Pathology 2023年第4期13卷 177-183页

作者： Javier ortiz Juan Carlos Paniagua Isidro Cavaco Raquel Rivas Andrea Jimenez Luis Chinchilla Elisa Muñoz María Dolores Ludeña departamento de Biología Celular y Patología Universidad de Salamanca (USAL) Salamanca Spain Servicio de Anatomía Patológica Hospital Universitario de Salamanca Salamanca Spain Servicio de Radiodiagnóstico Hospital Universitario de Salamanca Salamanca Spain

Objective: To describe the radiological, histological and immunohistochemical findings in a case of Progressive Multifocal Leukoencephalopathy (PML) affecting the cerebellar peduncles in a patient with chronic lymphocytic leukemia. Patient and Methods: Magnetic Resonance Imaging (MRI), histological picture (H.E., Kluver-Barrera) and immunohistochemical picture (GFAP, neurofilaments, CD68, jc virus) were obtained. Results: 1) Magnetic resonance imaging: Asymmetric and progressive lesions on middle cerebellar peduncles, that were hyperintense in T2/FLAIR, extended towards the pons, had no mass effect and were unmodified after intravenous contrast. 2) Histology: Marked reactive gliosis with cytopathic changes suggesting viral infection, plus demyelination areas with axonal preservation. 3) Immunohistochemistry: Marked positivity for viral (polyoma and jc virus) markers in glial cells showing cytopathic changes. Conclusions: The importance of histological and immunohistochemical diagnosis in everyday assistance;of the collaboration between clinicians, radiologists and pathologists;and the validity of postmortem studies as a key element for research and clinical quality assessment must be stressed.

关键词： Progressive Multifocal Leukoencephalopathy jc Virus Immunohistochemistry

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