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Giemsa and Grocott in the recognition of Histoplasma capsulatum in blood smears

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Asian Pacific Journal of Tropical Biomedicine 2013年第5期3卷 418-420页

作者： javier bava Alcides Troncoso Laboratory of Parasitology Infectious Diseases Muniz Hospital Department of Microbiology and Infectious Diseases School of MedicineUniversity of Buenos Aires

Objective:To facilitate the recognition of intracellular yeasts of Histoplasma capsulation and differentiate it from Leishmania amastigotes and other parasites,using the combination of Giemsa and a rapid modification of Grocott stains to peripheral blood smears in a hematological ***:The combination of both stains was applied consecutively(first Grocott and then Giemsa)to previously fixed peripheral blood *** was performed with 400x and 1000x,the latter using immersion ***:The yeasts of Histoplasma capsulatum were observed into the cytoplasm of leukocytes as brownish oval elements,with 3-4μm in ***:The combination of both techniques is a simple and fast method to facilitate recognition of intracellular yeasts and it is different from intracellular parasitic ***,it allows distinguishing the cell elements that are in the microscopic *** may be very helpful in those cases in which the presumptive diagnosis of histoplasmosis has not been established yet and where other more sophisticated methods are not available.

关键词： Histoplasmosis Peripheral blood smear Visceral leishmaniasis HIV

Adult female of strongyloides stercoralis in respiratory secretions

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Asian Pacific Journal of Tropical Biomedicine 2013年第4期3卷 311-313页

作者： bava Amadeo javier bava Domínguez Cecilia Troncoso Alcides Laboratory of Parasitology.Infectious Diseases Hospital "Francisco javier Muniz" Uspallata 2272.Buenos AiresArgentina Department of Infectious Diseases School of MedicineBuenos Aires UniversityArgentina

Objective:To communicate the presence of adult females,rabditoid larvae and eggs of Strongyloides stercoralis(***)in the respiratory secretions obtained by tracheal aspirate from a HIV-negative patient who was suffering from polymyositis,and treated with corticoids and amethopterin and assisted by ***:The respiratory secretions submitted to the Parasitology Laboratory of the Mu(?)iz Hospital were made more concentrated by centrifugation(1 500 r/min for 15 seconds).Wet mount microscopy was performed with the ***:It revealed adult females,rabditoid larvae and eggs of *** parasitological studies performed after the start of the treatment with ivermectin on fresh fecal samples,gastric lavages and tracheal aspirates showed scanty mobile filariform and rabditoid larvae of the same ***:The presence of adult female *** which has never been observed before in the clinical samples submitted to our Laboratory for investigation can be considered as an indirect marker of the severe immunosupression of the patient.

关键词： Strongyloidiasis Strongyloides stercoralis Hyperinfection Parasitological diagnosis Adult female

Pulmonary coinfection by Pneumocystis jiroveci and Cryptococcus neoformans

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Asian Pacific Journal of Tropical Biomedicine 2012年第1期2卷 80-82页

作者： bava javier Lloveras Susana Garro Santiago Troncoso Alcides Laboratory of Parasitology Infectious Diseases Hospital Division of Infectious Diseases Francisco Muniz Hospital Department of Infectious Diseases Tropical MedicineSchool of Medicine.Buenos Aires University

We communicate the diagnosis by microscopy of a pulmonary coinfection produced by Cryptococcus neoformans and Pneumocystis jiroveci,from a respiratory secretion obtained by bronchoalveolar lavage of an AIDS *** review of literature identified this coinfection as unusual *** infections associated with HIV infection are increasingly *** may occur at an early stage of *** concurrent opportunistic infections may occur,coexisting Pneumocystis jiroveci pneumonia(PCP)and disseminated cryptococcosis with cryptococcal pneumonia is *** lungs of individuals infected with HIV are often affected by opportunistic infections and tumours and over two-thirds of patients have at least one respiratory episode during the course of their *** is the leading HIV-associated *** present the case of a man who presented dual Pneumocystis jiroveci and cryptococcal pneumonia in a patient with *** diagnosis of PCP and Cryptococcus requires demonstration of these organisms in pulmonary tissues or *** patients with<200/microliter CD4-lymphocytes,a bronchoalveolar lavage should be *** patient was successfully treated with amphotericin B and trimethoprim *** 1 week the patient showed clinical and radiologic improvement and was discharged 3 weeks later.

关键词： Cryptococcus neoformans Pneumocystis jiroveci Pulmonary coinfection Diagnosis test Opportunistic pathogen Pneumonia Definitive diagnosis

A case report of cutaneous larva migrans in Argentina

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Asian Pacific Journal of Tropical Biomedicine 2011年第1期1卷 81-82页

作者： javier bava Lucia G Gonzalez Celeste M Seley Gisela P Lopez Alcides Troncoso Infectious Diseases "Francisco J Mui" Department of Microbiology ParasitologyInfectious Diseases and Tropical MedicineSchool of MedicineBuenos Aires University

Cutaneous larva migrans(CLM) represents the most common tropically acquired *** is caused by infection with hookworm larvae in tropical and sub-tropical areas,and people who have a history of foreign travel and of walking barefoot on sandy soil or beaches are at a high risk of getting infected with *** diagnosis is usually made on the basis of the typical appearance of the lesion,intense itching and history of foreign *** is a common parasitic skin disease that can be easily prevented by wearing 'protective' footwear.A case of CLM is described in this article.

关键词： Cutaneous larva migrans Epidermal parasitic skin diseases Dog faeces Hookworm larvae Protective footwear

Astrocytes, reactive astrogliosis, and glial scar formation in traumatic brain injury

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Neural Regeneration Research 2025年第4期20卷 973-989页

作者： María Belén Cieri Alberto javier Ramos Laboratorio de Neuropatología Molecular IBCN UBA-CONICETFacultad de MedicinaUniversidad de Buenos AiresBuenos AiresArgentina

Traumatic brain injury is a global health crisis,causing significant death and disability *** that follows traumatic brain injury has serious consequences for neuronal survival and cognitive impairments,with astrocytes involved in this *** traumatic brain injury,astrocytes rapidly become reactive,and astrogliosis propagates from the injury core to distant brain *** astroglial proteins are downregulated near the traumatic brain injury core,while pro-inflammatory astroglial genes are *** altered gene expression is considered a pathological remodeling of astrocytes that produces serious consequences for neuronal survival and cognitive *** addition,glial scar formed by reactive astrocytes is initially necessary to limit immune cell infiltration,but in the long term impedes axonal reconnection and functional *** therapeutic strategies for traumatic brain injury are focused on preventing acute ***,cannabinoids,progesterone,beta-blockers,and cerebrolysin demonstrate neuroprotective benefits but most of them have not been studied in the context of *** this review,we discuss the cell signaling pathways activated in reactive astrocytes following traumatic brain injury and we discuss some of the potential new strategies aimed to modulate astroglial responses in traumatic brain injury,especially using cell-targeted strategies with miRNAs or lncRNA,viral vectors,and repurposed drugs.

关键词： astrocyte glial scar innate immunity neuroinflammation stab injury Toll-like receptors

Beyond neurodegeneration:engineering amyloids for biocatalysis

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Neural Regeneration Research 2025年第10期20卷 2915-2916页

作者： Andrea Bartolomé-Nafría javier García-Pardo Salvador Ventura Institut de Biotecnologia i de Biomedicina(IBB)and Departament de Bioquímica i Biologia Molecular Universitat Autònoma de BarcelonaBellaterraBarcelonaSpain Hospital Universitari Parc Taulí Institut d’Investigaciói InnovacióParc Taulí(I3PT-CERCA)Universitat Autònoma de BarcelonaSabadellSpain

Amyloid fibrils are highly organized protein or peptide aggregates,often characterized by a distinctive supramolecular cross-β-sheet *** formation and accumulation of these structures have been traditionally associated with neural or systemic human diseases,such as Alzheimer’s disease,Parkinson’s disease,type-2 diabetes,or amyotrophic lateral sclerosis(Wei et al.,2017;Wittung-Stafshede,2023).

关键词： structure amyloid

Leishmaniasis in the knee area

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Asian Pacific Journal of Tropical Medicine 2010年第4期3卷 330-331页

作者： bava Amadeo javier Rossi Maria Laura Seley Celeste Troncoso Alcides Department of Micology La Plata University Department of Microbiology Parasitology and Infectious DiseasesSchool of MedicineBuenos Aires University

Leishmaniasis is a parasitic infection caused by species leishmaniae,which can produce two types of manifestations:visceral and *** south America cutaneous leishmaniasis is more common than visceral leishmaniasis.A case of primary cutaneous leishmaniasis from Bolivia is presented for its *** patient of our case showed an ulcerated lesion of the knee. Montenegro’s intradermal test was ***-stained touch preparation of the skin biopsy revealed amastigotes inside macrophages,consistent with *** patient was treated with meglumine antimoniate intramuscular(20 mg of Sb+/kg/day) three weeks,with complete cicatrization of the lesion.

关键词： Cutaneous leishmaniasis Skin ulcers Leishmania

Chromoblastomycosis as a problem of the tropics

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Asian Pacific Journal of Tropical Medicine 2009年第3期2卷 79-80页

作者： bava Amadeo javier Rodriguez Rios Elizabeth Troncoso Alcides Department of Micology La Plata National UniversityBuenos Aires State Department of Microbiology Parasitology and Infectious DiseasesSchool of MedicineBuenos Aires University

Chromoblastomycosis can also cause severe but usually superficial lesions on the *** other deep mycosis, this chronic and disabling condition is seen in many tropical regions,including Argentina,where this man was ***... 详细信息

关键词： Chromoblastomycosis Verrucous Lesiones

Differential response of injured and healthy retinas to syngeneic and allogeneic transplantation of a clonal cell line of immortalized olfactory ensheathing glia:a double-edged sword

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Neural Regeneration Research 2025年第8期20卷 2395-2407页

作者： María Norte-Muñoz María Portela-Lomba Paloma Sobrado-Calvo Diana Simón Johnny Di Pierdomenico Alejandro Gallego-Ortega Mar Pérez JoséMCabrera-Maqueda javier Sierra Manuel Vidal-Sanz María Teresa Moreno-Flores Marta Agudo-Barriuso Grupo de Investigación Oftalmología Experimental Departamento de OftalmologíaOptometríaOtorrinolaringología y Anatomía PatológicaFacultad de MedicinaUniversidad de MurciaInstituto Murciano de Investigación Biosanitaria(IMIB)Campus de Ciencias de la SaludMurciaSpain Experimental Sciences Faculty Universidad Francisco de VitoriaPozuelo de AlarcónMadridSpain Anatomy Histology and Neuroscience DepartmentMedicine FacultyUniversidad Autónoma de MadridMadridSpain Center of Neuroimmunology Service of NeurologyLaboratory of Advanced Imaging in Neuroimmunological DiseasesHospital Clínic of BarcelonaInstitut d’Investigacions Biomèdiques August Pi i Sunyer(IDIBAPS)and Universitat de BarcelonaBarcelonaSpain Medicine Faculty Universidad Francisco de VitoriaPozuelo de AlarcónMadridSpain

Olfactory ensheathing glia promote axonal regeneration in the mammalian central nervous system,including retinal ganglion cell axonal growth through the injured optic ***,it is unknown whether olfactory ensheathing glia also have neuroprotective *** ensheathing glia express brain-derived neurotrophic factor,one of the best neuroprotectants for axotomized retinal ganglion ***,we aimed to investigate the neuroprotective capacity of olfactory ensheating glia after optic nerve *** ensheathing glia cells from an established rat immortalized clonal cell line,TEG3,were intravitreally injected in intact and axotomized retinas in syngeneic and allogeneic mode with or without microglial inhibition or immunosuppressive *** and gene expression analyses were *** bulb-derived primary olfactory ensheathing glia and TEG3 express major histocompatibility complex classⅡ*** and syngenically transplanted TEG3 cells survived in the vitreous for up to 21 days,forming an *** axotomized retinas,only the allogeneic TEG3 transplant rescued retinal ganglion cells at 7 days but not at 21 *** these retinas,microglial anatomical activation was higher than after optic nerve crush *** intact retinas,both transplants activated microglial cells and caused retinal ganglion cell death at 21 days,a loss that was higher after allotransplantation,triggered by pyroptosis and partially rescued by microglial inhibition or ***,neuroprotection of axotomized retinal ganglion cells did not improve with these *** different neuroprotective properties,different toxic effects,and different responses to microglial inhibitory treatments of olfactory ensheathing glia in the retina depending on the type of transplant highlight the importance of thorough preclinical studies to explore these variables.

关键词： cell therapy immune recognition major histocompatibility complex class II(MHCII) neuroprotection olfactory ensheathing glia retinal ganglion cells