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A key‘foxy’aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape‘Concord’

Horticulture Research 2020年第1期7卷 2028-2039页

作者： Yingzhen Yang JoséCuenca Nian Wang Zhenchang Liang Honghe Sun Benjamin Gutierrez Xiaojun Xi Jie Arro YiWang Peige Fan jason Londo Peter Cousins Shaohua Li Zhangjun Fei Gan-Yuan Zhong US Department of Agriculture-Agricultural Research Service Grape Genetics Research UnitGenevaNYUSA Beijing Key Laboratory of Grape Sciences and enology Laboratory of Plant ResourcesInstitute of BotanyChinese Academy of SciencesBeijingChina Boyce Thompson Institute for Plant Research Cornell UniversityIthacaNYUSA US Department of Agriculture-Agricultural Research Service Plant Genetic Resources UnitGenevaNYUSA Forestry and Pomology Research Institute Shanghai Academy of Agricultural SciencesShanghaiChina University of Chinese Academy of Sciences BeijingChina e.&J.Gallo Winery ModestoCAUSA US Department of Agriculture–Agricultural Research Service Robert W.Holley Center for Agriculture and HealthIthacaNYUSA Present address:Centro de Citricultura y Producción Vegetal.Instituto Valenciano de Investigaciones Agrarias MoncadaValenciaSpain Present address:College of Horticulture and Forestry Huazhong Agricultural UniversityWuhanHubeiChina

‘Concord’,the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L.,possesses a special‘foxy’aroma predominantly resulted from the accumulation of methyl anthranilate(MA)in *** aroma,however,is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species *** we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key‘foxy’aroma gene,anthraniloyl-CoA:methanol acyltransferase(AMAT),that is responsible for MA *** found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in‘Concord’and other ***-derived *** promoter variants,all with direct and inverted repeats,were further confirmed in more than 1,300 Vitis ***,functional impact of these indels was validated in transgenic *** on the promoter regulation,large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some *** *** of the AMAT genetic regulation advances our understanding of the‘foxy’aroma trait and makes it genetically trackable and amenable in grapevine breeding.

关键词： insertion aroma grape

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Genetic and environmental control of fruit maturation,dry matter and firmness in apple(Malus×domestica Borkh.)

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Horticulture Research 2014年第1期1卷 98-109页

作者： David Chagne Daya Dayatilake Robert Diack Murray Oliver Hilary Ireland Amy Watson Susan e Gardiner jason W Johnston Robert J Schaffer Stuart Tustin The New Zealand Institute for Plant&Food Research Limited(Plant&Food Research) Palmerston North Research CentrePalmerston NorthNew Zealand Plant&Food Research Hawke’s Bay Research CentreHavelock NorthNew Zealand Plant&Food Research Motueka Research CentreMotuekaNew Zealand Plant&Food Research Mount Albert Research CentreAucklandNew Zealand School of Biological Sciences University of AucklandAucklandNew Zealand

For any given genotype,the environment in which an apple is grown can influence the properties of the fruit *** there has been extensive research on the mechanism of the genetic control of fruit quality traits,less effort has been made to investigate the way that these genetic mechanisms interact with the *** address this issue,we employed a large‘Royal Gala’×‘Braeburn’population of 572 seedlings replicated over sites in three climatically diverse apple-growing regions in New *** for traits including fruit maturation timing,firmness and dry matter content was performed at each of these three sites for a single growing season(2011),and at two sites(Motueka and Hawke’s Bay)for two seasons(2009 and 2010).The phenotype data collected over 2 years at two sites enabled the detection of 190 quantitative trait loci(QTL)that controlled these traits regardless of year or growing location,as well as some chromosomal loci that influenced the traits in a single given environment or *** those loci that were environmentally stable over three sites,there was an interdependency of fruit maturation date,dry matter content and storage potential within this population,with two regions on Linkage Groups(LGs)10 and 16 strongly *** these loci were used in a marker-assisted selection programme to select for progeny bearing firmer fruit,this would have the unintentional consequence of selecting,high dry matter content,later maturing *** addition,a further 113 new QTLs with a smaller effect were identified,some of which were exhibited only in a single growing environment,demonstrating the underlying complexity of control of traits determining fruit quality,in addition to the need for being aware of environmental effects when developing new apple varieties.

关键词： fir apple fruit

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Are there differences in anaerobic relative muscle power between upper and lower limbs in adolescent swimmers:A blinded study

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Sports Medicine and Health Science 2023年第4期5卷 290-298页

作者： Paulo Francisco de Almeida-Neto Adam Baxter-Jones jason Azevedo de Medeiros Paulo Moreira Silva Dantas Breno Guilherme de Araújo Tinoco Cabral Health Sciences Center Federal University of Rio Grande do NorteUFRNNatalRNBrazil Department of Physical education Federal University of Rio Grande do NorteUFRNNatalRNBrazil College of Kinesiology University of SaskatchewanSaskatoonSKS7N 5B2Canada

Success in speed swimming depends on the efficiency of the anaerobic system for the production of cellular energy,especially during muscle power *** the adolescent athletes much is unknown with regards to the relationships between relative power of upper and lower limbs with speed swimming *** aim the present study was to identify differences in relative muscle power of upper and lower limbs in adolescent swimmers and relate these to speed swimming *** adolescents,of both sexes(50%female,50%male,30 swimmers and 30 controls),were *** relative upper limb power(ULP[W/kg])was assessed by a medicine ball test and the relative lower limbs power(LLP[W/kg])by a jump test on a jumping *** mass of the upper and lower limbs was assessed by dual-energy X-ray absorptiometry(DXA)(g).Sport performance was assessed during national level competition(50-m swimming time[in seconds]).Biological maturation(BM)was indexed by years from attainment of peak height ***(W/kg)was higher than LLP(W/kg)in both groups(per and lower limb lean mass(g)correlated significantly with ULP(W/kg)and LLP(W/kg)in both groups(pelated with 50-m swimming performance(s),in both sexes(ped BM was associated with ULP(W/kg)and LLP(W/kg)in both groups(performance(s)in both sexes(pe concluded that ULP(W/kg)is higher than LLP(W/kg)in adolescent *** and lower limb lean mass and BM were both positively associated with increased ULP(W/kg)and LLP(W/kg).

关键词： Performance Sport Swimming Biological maturation

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Investigating Women’s Realization on Understanding the Impact of Climate Variability in Socioeconomic Performance in Zanzibar, Tanzania: A Case of Jambiani Village

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Atmospheric and Climate Sciences 2022年第2期12卷 313-329页

作者： Bibie Salim Abdullah Kombo Hamad Kai jason Kalugendo Shamim Mushi Veronica Mgalula Decatur Georgia USA Tanzania Meteorological Authority (TMA) Dar es Salaam Zanzibar Tanzania

The study employed the triangulation approach to investigate the impact of climate variability on selected socioeconomic indicators, particularly food production, health and education, and how much climate variability and its impact on those indicators was realized and understood among the women living in Jambiani community in Zanzibar. The study used the explanatory, sequential mixed method to collect, datasets related to Jambiani communities (i.e. from the women in Kibigija village), other climate data acquired from Tanzania Meteorological Authority (TMA), Ministries of Agriculture and Health and Southern District in Zanzibar. The results obtained from the analysis of both anecdotal (interview, questionnaire among others) data shows that climate variability was imminent in Jambiani communities between 2010 and 2015 and had a negative impact on food production, health and education. Further results revealed that women working in informal sectors are more vulnerable to climate impacts than women working in formal sectors due to less knowledge and techniques to mitigate/cope with climate variability impacts. Conclusively the study has shown that climate variability affects more women with less awareness of what is happening and how to cope with it, thus the study calls for new research work and increasing the awareness on the impacts of climate on human basic needs.

关键词： Climate Variability Women Coping Mechanism Zanzibar

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Clarifying sub-genomic positions of QTLs for flowering habit and fruit quality in U.S. strawberry (Fragaria×ananassa) breeding populations using pedigree-based QTL analysis

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Horticulture Research 2017年第1期4卷 85-93页

作者： Sujeet Verma jason D Zurn Natalia Salinas Megan M Mathey Beatrice Denoyes James F Hancock Chad e Finn Nahla V Bassil Vance M Whitaker IFAS/Department of Horticulture University of FloridaGulf Coast Research and Education Center14625 CR 672Wimauma33598FLUSA USDA-ARS National Clonal Germplasm Repository 33447 Peoria RoadCorvallis97333ORUSA Department of Horticulture Oregon State University4017 Agriculture and Life Sciences BuildingCorvallis97331ORUSA Department of Horticulture UMR 1332 Biologie du Fruit et PathologieINRAUniv.BordeauxVillenave d’OrnonF-33140France Michigan State University East Lansing48824MIUSA USDA-ARS HCRU3420 NW Orchard AvenueCorvallis97330ORUSA

The cultivated strawberry(Fragaria×ananassa)is consumed worldwide for its flavor and nutritional *** analysis of commercially important traits in strawberry are important for the development of breeding methods and tools for this *** several quantitative trait loci(QTL)have been previously detected for fruit quality and flowering traits using low-density genetic maps,clarity on the sub-genomic locations of these QTLs was *** discoveries in allo-octoploid strawberry genomics led to the development of the IStraw90 single-nucleotide polymorphism(SNP)array,enabling high-density genetic maps and finer resolution QTL *** this study,breeder-specified traits were evaluated in the eastern(Michigan)and Western(Oregon)United States for a common set of breeding populations during 2 *** QTLs were validated for soluble solids content(SSC),fruit weight(FWT),pH and titratable acidity(TA)using a pedigree-based QTL analysis *** fruit quality,a QTL for SSC on linkage group(LG)6A,a QTL for FWT on LG 2BII,a QTL for pH on LG 4CII and two QTLs for TA on LGs 2A and 5B were *** addition,a large-effect QTL for flowering was detected at the distal end of LG 4A,coinciding with the FaPFRU *** haplotype analysis in the FaPFRU region indicated that the homozygous recessive genotype was highly predictive of seasonal *** probes in the FaPFRU region may help facilitate marker-assisted selection for this trait.

关键词： breeding straw Fragaria

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Mechanistic insights into the key marine dimethylsulfoniopropionate synthesis enzyme DsyB/DSYB

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mLife 2022年第2期1卷 114-130页

作者： Chun-Yang Li jason C.Crack Simone Newton-Payne Andrew R.J.Murphy Xiu-Lan Chen Benjamin J.Pinchbeck Shun Zhou Beth T.Williams Ming Peng Xiao-Hua Zhang Yin Chen Nick e.Le Brun Jonathan D.Todd Yu-Zhong Zhang Frontiers Science Center for Deep Ocean Multispheres and earth System College of Marine Life SciencesOcean University of ChinaQingdaoChina State Key Laboratory of Microbial Technology Marine Biotechnology Research CenterShandong UniversityQingdaoChina Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and TechnologyQingdaoShandongChina School of Chemistry Centre for Molecular and Structural BiochemistryUniversity of East AngliaNorwich Research ParkNorwichUK School of Biological Sciences University of East AngliaNorwichUK School of Life Sciences University of WarwickCoventryUK

Marine algae and bacteria produce approximately eight billion tonnes of the organosulfur molecule dimethylsulfoniopropionate(DMSP)in earth's surface oceans *** is an antistress compound and,once released into the environment,a major nutrient,signaling molecule,and source of climate-active *** methionine transamination pathway for DMSP synthesis is used by most known DMSP-producing algae and *** S-directed S-adenosylmethionine(SAM)-dependent 4-methylthio-2-hydroxybutyrate(MTHB)S-methyltransferase,encoded by the dsyB/DSYB gene,is the key enzyme of this pathway,generating S-adenosylhomocysteine(SAH)and 4-dimethylsulfonio-2-hydroxybutyrate(DMSHB).DsyB/DSYB,present in most haptophyte and dinoflagellate algae with the highest known intracellular DMSP concentrations,is shown to be far more abundant and transcribed in marine environments than any other known S-methyltransferase gene in DMSP synthesis ***,we demonstrate in vitro activity of the bacterial DsyB enzyme from Nisaea denitrificans and provide its crystal structure in complex with SAM and SAH-MTHB,which together provide the first important mechanistic insights into a DMSP synthesis *** and mutational analyses imply that DsyB adopts a proximity and desolvation mechanism for the methyl transfer *** analysis suggests that this mechanism may be common to all bacterial DsyB enzymes and also,importantly,eukaryotic DSYB enzymes from e.g.,algae that are the major DMSP producers in earth's surface oceans.

关键词： catalytic mechanism DMSP synthesis marine sulfur cycle S-methyltransferase

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elective oocyte freezing for the preservation of fertility

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Open Journal of Obstetrics and Gynecology 2012年第1期2卷 27-33页

作者： jason Barritt Martha Luna Benjamin Sandler Marlena Duke Alan B. Copperman Reproductive endocrinology Mount Sinai School of Medicine New York USA Reproductive Medicine Associates of New York New York USA

Oocyte cryopreservation has recently emerged as an option for women to preserve their fertility for medical (e.g. treatable malignancy) or elective indications (e.g. advancing age). This report describes an IRB-approved study of over 200 oocyte cryopreservation cycles at one center. Patients presenting for oocyte cryopreservation (January 2005 to 2010) were analyzed for day 3 follicle stimulating hormone (FSH), basal antral follicle count (BAFC), gonadotropin usage and the number of oocytes retrieved and cryopreserved. New patient consultations were performed on 516 women, of whom 175 (34%) proceeded to initiate a total of 233 cryopreservation cycles. Twenty-four cycles were cancelled (10%) after starting follicular stimulation due to poor ovarian response or self-withdrawal of the patients. Patients whose cycles were cancelled demonstrated a higher Day 3 FSH and a lower BAFC than patients who completed cycles (p e 209 completed cycles, the most important predictors of a successful cycle included BAFC (r = 0.36), FSH (r = –0.25) and age (r = –0.18) with the mean number of oocytes cryopreserved at 13.6 ± 8.8. Information about long-term fertility preservation must reach both patients and health care providers so that more women can be educated about the benefits of proactive early physiological reproductive assessment and possible interventions available.

关键词： Human Oocyte Cryopreservation Fertility Preservation Oocyte Aging Ovarian Reserve Follicle Stimulating Hormone Basal Antral Follicle Count

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Mobile Infrastructure for Monitoring, Modeling, and Forecasting of Coastal Weather events. Phase I: Building the Smart Drifter

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Journal of Computer and Communications 2019年第7期7卷 85-94页

作者： Jiannan Zhai jason O. Hallstrom Shaowu Bao Paul Gayes Len Pietrafesa Institute for Sensing and embedded Network Systems engineering Florida Atlantic University Boca Raton USA Coastal and Marine Systems Science Coastal Carolina University Conway South Carolina USA

Our goal is to address a critical need to improve monitoring and forecasting of storm-induced coastal flooding (i.e., storm surge). The fundamental element of our approach is a new class of smart drifter—low-cost, rapidly deployable sensors used to collect surge information in advance of, during, and following significant weather events. These devices, each a bit larger than a tennis ball, will be deployed in advance of oncoming storms. The devices will log surge information used for post processing. In this paper, we present the Phase I of the project: the design and development of the smart drifter.

关键词： Surge Monitoring Smart Drifter

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在休斯顿使用多传感器检查使管道破裂风险最小化

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非开挖技术 2009年第5期26卷 45-46页

作者： jason Iken P.e., Wayne Brown 王轲（译）胡远彪（校）

由于高成本和极具破坏性的特性，紧急管道修复成为被迫选择的措施。1992年，由于糟糕的土质和管道接头的泄露，造成休斯顿Almeda路40英尺长的一段路面发生了塌陷，需要紧急的管道修复。此后，休斯顿公共工程和事业部的工程师立即准备调... 详细信息

由于高成本和极具破坏性的特性，紧急管道修复成为被迫选择的措施。1992年，由于糟糕的土质和管道接头的泄露，造成休斯顿Almeda路40英尺长的一段路面发生了塌陷，需要紧急的管道修复。此后，休斯顿公共工程和事业部的工程师立即准备调查13英里的大直径废水管道设施。工程师非常关注NSRT（北部地区清洁改善隧道）段，这段管道带着废水从北部穿过市中心到达该市最大的废水处理厂。当时，大直径管道的调查只能选择人工检查的方式。

关键词：传感器检查管道破裂休斯顿最小化废水处理厂大直径管道风险管道修复

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Levels of Chemical Toxicants in Waterpipe Tobacco and Waterpipe Charcoal Solid Waste

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Journal of environmental Protection 2021年第11期12卷 913-938页

作者： jason R. Hsieh Megan L. Mekoli Ronald L. edwards Jr. Office of Science Center for Tobacco Products U.S. Food and Drug Administration Calverton MD USA

This work provides insights on waterpipe tobacco and waterpipe charcoal as potential sources of environmental toxicants. Selected harmful and potentially harmful constituents (HPHCs) from ten U.S. commercial waterpipe tobacco filler products (before and after electric heating) and five waterpipe charcoal products (before and after burning) were investigated. The differences in quantities of HPHCs between the evaluated products appear to be affected by raw material properties and/or the manufacturing processes involved in product production. Trace metal quantities in waterpipe tobacco and charcoal products were observed after heating or burning conditions compared to unheated or unburned conditions, which could impact the environment through the generation of toxic tobacco product waste. This study demonstrates that waterpipe tobacco and waterpipe charcoal contain substantial quantities of benzo[a]pyrene (B[a]P) and trace metals (i.e., selenium, arsenic, cadmium, chromium, cobalt, lead, nickel) before use and that extensive and varied changes in trace metal quantities take place as a result of heating, and more studies are needed to estimate the magnitude of the environmental impact of waterpipe tobacco use.

关键词： Harmful and Potentially Harmful Constituents Charcoal Waterpipe Metals Tobacco Product Waste

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