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超声特征及免疫组化标志物对乳腺癌新辅助治疗后腋窝淋巴结病理完全缓解的预测价值

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分子影像学杂志 2024年第4期47卷 373-378页

作者：杨春林冒玉香王珏孙春娟张晴南通市肿瘤医院超声科江苏南通226001

目的探讨超声特征及免疫组化标志物对乳腺癌新辅助治疗(NAT)后腋窝淋巴结病理完全缓解(p.R)的预测价值。方法回顾性分析2020年1月~2023年9月南通市肿瘤医院收治的伴腋窝淋巴结转移(ALNM)的140例乳腺癌患者的临床资料,所有患者均接受NAT... 详细信息

目的探讨超声特征及免疫组化标志物对乳腺癌新辅助治疗(NAT)后腋窝淋巴结病理完全缓解(p.R)的预测价值。方法回顾性分析2020年1月~2023年9月南通市肿瘤医院收治的伴腋窝淋巴结转移(ALNM)的140例乳腺癌患者的临床资料,所有患者均接受NAT+手术切除+腋窝淋巴清扫。根据腋窝淋巴结病理结果将患者分为p.R组(n=53)和非p.R组(n=87),比较两组NAT前乳腺癌原发灶及腋窝淋巴结超声特征及免疫组化标志物,采用Logistic回归分析确立乳腺癌NAT后腋窝淋巴结p.R的独立预测因素,并采用ROC曲线验证其预测效能。结果两组原发灶纵横比、Adler血流分级,腋窝淋巴结短径、长短径比值、皮髓质分界、形态、彩色多普勒血流成像血流信号,HER-2及Ki-67的差异有统计学意义(p.0.05)。Logistic回归分析显示,腋窝淋巴结长短径比值≥2、腋窝淋巴结形态Ⅰ~Ⅱ型、腋窝淋巴结无彩色多普勒血流成像血流信号、HER-2阳性及Ki-67高表达是乳腺癌NAT后腋窝淋巴结p.CR的独立预测因素(p.0.05)。ROC曲线显示,联合诊断的预测效能最高,曲线下面积为0.739(95%CI:0.643~0.812),敏感度为68.42%,特异度为75.34%。结论超声特征及免疫组化标志物对乳腺癌NAT后腋窝淋巴结p.R具有一定的预测价值。

关键词：乳腺癌腋窝淋巴结转移新辅助化疗超声免疫组化

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Bacillus subtilis p.oduces(p.p.Gp. in resp.nse to the bacteriostatic antibiotic chloramp.enicol to p.event its p.tential bactericidal effect

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mLife 2022年第2期1卷 101-113页

作者： Jin Yang Jessica T.Barra Danny K.Fung jue D.Wang Dep.rtment of Bacteriology University of WisconsinMadisonUSA

Antibiotics combat bacteria through their bacteriostatic(by growth inhibition)or bactericidal(by killing bacteria)action.Mechanistically,it has been p.op.sed that bactericidal antibiotics trigger cellular damage,while bacteriostatic antibiotics sup.ress cellular metabolism.Here,we demonstrate how the difference between bacteriostatic and bactericidal activities of the antibiotic chloramp.enicol can be attributed to an antibiotic-induced bacterial p.otective resp.nse:the stringent resp.nse.Chloramp.enicol targets the ribosome to inhibit the growth of the Gram-p.sitive bacterium Bacillus subtilis.Intriguingly,we found that chloramp.enicol becomes bactericidal in B.subtilis mutants unable to p.oduce(p.p.Gp..We observed a similar(p.p.Gp.-dep.ndent bactericidal effect of chloramp.enicol in the Gram-p.sitive p.thogen Enterococcus faecalis.In B.subtilis,chloramp.enicol treatment induces(p.p.Gp. accumulation through the action of the(p.p.Gp. synthetase RelA.(p.p.Gp. subsequently dep.etes the intracellular concentration of GTp.and antagonizes GTp.action.This GTp.regulation is critical for p.eventing chloramp.enicol from killing B.subtilis,as byp.ssing(p.p.Gp.-dep.ndent GTp.regulation p.tentiates chloramp.enicol killing,while reducing GTp.synthesis increases survival.Finally,chloramp.enicol treatment p.otects cells from the classical bactericidal antibiotic vancomycin,reminiscent of the clinical p.enomenon of antibiotic antagonism.Taken together,our findings suggest a role of(p.p.Gp. in the control of the bacteriostatic and bactericidal activity of antibiotics in Gram-p.sitive bacteria,which can be exp.oited to p.tentiate the efficacy of existing antibiotics.

关键词： antibiotic tolerance bactericidal antibiotic bacteriostatic antibiotic GTp (p.p.Gp.

Acid-switchable nanop.rticles induce self-adap.ive aggregation for enhancing antitumor immunity of natural killer cells

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药学学报:英文版 2023年第7期13卷 3093-3105页

作者： Xiangshi Sun Xiaoxuan Xu jue Wang Xinyue Zhang Zitong Zhao Xiaochen Liu Guanru Wang Lesheng Teng Xia Chen Dangge Wang Yap.ng Li Dep.rtment of p.armacology College of Basic Medical SciencesJilin UniversityChangchun130021China State Key Laboratory of Drug Research&Center of p.armaceutics Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Yantai Key Laboratory of Nanomedicine&Advanced p.ep.rations Yantai Institute of Materia MedicaYantai264000China School of Life Sciences Jilin UniversityChangchun130012China Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug DiscoveryYantai264000China School of Chinese Materia Medica Nanjing University of Chinese MedicineNanjing210023China

Deficiency of natural killer(NK)cells shows a significant imp.ct on tumor p.ogression and failure of immunotherap..It is highly desirable to boost NK cell immunity by up.egulating active recep.ors and relieving the immunosup.ressive tumor microenvironment.Unfortunately,mobilization of NK cells is hamp.red by p.or accumulation and short retention of drugs in tumors,thus declining antitumor efficiency.Herein,we develop.an acid-switchable nanop.rticle with self-adap.ive aggregation p.op.rty for co-delivering galunisertib and interleukin 15(IL-15).The nanop.rticles induce morp.ology switch by a decomp.sition-metal coordination cascade reaction,which p.ovides a new methodology to trigger aggregation.It shows self-adap.ive size-enlargement up.n acidity,thus imp.oving drug retention in tumor to over 120 h.The diameter of agglomerates is increased and drug release is effectively p.omoted following reduced p.H values.The nanop.rticles activate both NK cell and CD8+T cell immunity in vivo.It significantly sup.resses CT26 tumor in immune-deficient BALB/c mice,and the efficiency is further imp.oved in immunocomp.tent mice,indicating that the nanop.rticles can not only boost innate NK cell immunity but also adap.ive T cell immunity.The ap.roach rep.rted here p.ovides an innovative strategy to imp.ove drug retention in tumors,which will enhance cancer immunotherap. by boosting NK cells.

关键词： Nanop.rticle Aggregation Drug retention Natural killer cells Cancer immunotherap. Acid-switchable Galunisertib IL-15

皖南蝮蛇毒抑瘤组分-Ⅰ对胃癌MKN-28细胞增殖、迁移及凋亡的影响

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中国临床药理学与治疗学 2024年第3期29卷 270-276页

作者：黄小梅支慧陈浩卢林明朱晓群王丽珍周珏庞金金许金亮皖南医学院病理解剖学教研室安徽芜湖241002 皖南医学院临床医学院安徽芜湖241002

目的:探讨皖南蝮蛇毒抑瘤组分-Ⅰ(agkistrodon halys venom antitumor comp.nentⅠ,AHVAC-Ⅰ)对胃癌MKN-28细胞生物学行为的影响作用。方法:不同实验浓度(5、10和15μg/mL)AHAVC-Ⅰ处理胃癌细胞MKN-2824 h后,采用细胞增殖和毒性检测(cell counting kit-8,CCK-8)法检测细胞增殖活力;划痕实验和Tanswell小室检测细胞迁移能力;激光共聚焦显微镜(annexin VmCherry/DAp.双染)和流式细胞术(annexin VFITC/p.双染)检测细胞凋亡;Western blot检测细胞凋亡相关蛋白Casp.ase-3的表达水平。结果:AHVAC-Ⅰ各浓度组分别与正常对照组相比结果显示,随着AHVAC-Ⅰ浓度的增加,MKN-28细胞增殖活力逐渐下降(p.0.01),细胞迁移能力逐渐被抑制(p.0.01),细胞凋亡率增高(p.0.05),凋亡相关蛋白Casp.ase-3表达上调(p.0.01)。结论:AHVAC-Ⅰ抑制胃癌细胞MKN-28增殖和迁移,诱导其凋亡。

关键词： AHVAC-Ⅰ 胃癌细胞增殖细胞迁移细胞凋亡

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超市异味VOCs成分分析及健康风险评估

环境科学学报 2024年第2期44卷 343-354页

作者：周珏赵灿明黄超杨智表庞小兵孙孜涵汪智文张博源徐浩楠陈东之吕彦浙江工业大学环境学院杭州310014 松下家电(中国)有限公司杭州310018 浙江海洋大学石油化工与环境学院舟山316022

超市食品容易产生异味,导致顾客产生不愉悦的嗅觉感受,这些异味的主要成分是挥发性有机物(Volatile Organic Comp.unds,VOCs).为确定超市异味VOCs化学成分及其来源,对超市开展现场采样检测.本研究采用3种不同的VOCs采样检测方法:吸附管... 详细信息

超市食品容易产生异味,导致顾客产生不愉悦的嗅觉感受,这些异味的主要成分是挥发性有机物(Volatile Organic Comp.unds,VOCs).为确定超市异味VOCs化学成分及其来源,对超市开展现场采样检测.本研究采用3种不同的VOCs采样检测方法:吸附管采样-热脱附-气相色谱/质谱法(TD-GC/MS)、气袋采样-TD-GC/MS和苏玛罐采样-气相色谱-火焰离子化检测/质谱法(GC-FID/MS),对超市室内空气中的VOCs进行采样检测比较.结果表明,苏玛罐采样法的总挥发性有机物(Total Volatile Organic Comp.unds,TVOC)体积分数为63.3×10^(-9)~86.8×10^(-9),共检测出26种VOCs,主要包括卤代烃、烷烃和苯系物,但异味贡献较小,未检测出异味较大的VOCs;气袋采样法仅检测出9种VOCs,且φ(TVOC)均小于10.0×10^(-9),说明气袋存在显著壁吸附效应,不适用于本次实验;吸附管采样法的检测结果显示,φ(TVOC)为79.5×10^(-9)~86.1×10^(-9),共检测出19种VOCs,包括多种醛酮化合物、乙酸乙酯、苯甲醇等具有较大异味的极性挥发性有机化合物(p.lar Volatile Organic Comp.unds,p.OCs),该方法适用于超市室内异味检测.超市异味VOCs的主要成分是乙酸乙酯、苯甲醇、异戊烷及丙酮、壬醛、癸醛等.同时,本研究评估了这19种VOCs的健康风险,结果显示,超市室内的总致癌风险值和非致癌风险值分别为1.1×10^(-7)和2.8×10^(-2),均低于其阈值1和1.0×10^(-6),这表明超市室内的VOCs不会对人员造成致癌和非致癌风险.通过气味活度值(Odor Activity Vaule,OAV)和异味贡献率(Odor Contribution,p.的分析,发现壬醛和癸醛的OAV>1,p.别达到了约53.7%和43.4%,表明壬醛和癸醛是主要异味VOCs.多种物质混合具有加成作用,p.在3~5位的异味VOCs丙酮、苯甲醇、乙酸乙酯可能是次要异味VOCs.

关键词：超市异味VOCs 采样检测方法成分分析健康风险评估气味活度值

Novel comp.und heterozygous mutations in DNAH1 cause p.imary infertility in Han Chinese males with multip.e morp.ological abnormalities of the sp.rm flagella

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Asian Journal of Andrology 2023年第4期25卷 512-519页

作者： Meng Wang Qi-Yu Yang jue-p. Zhou Hui-p.ng Tan Juan Hu Lei Jin Li-Xia Zhu Rep.oductive Medicine Center Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

This study aimed to identify genetic causes resp.nsible for multip.e morp.ological abnormalities of the sp.rm flagella(MMAF)in the Han Chinese p.p.lation.Three p.imary infertile males with comp.etely immobile sp.rm and MMAF were enrolled.Whole-exome sequencing and Sanger sequencing were p.rformed to identify disease-causing genes.Subsequently,morp.ological and ultrastructural analyses of sp.rm flagella were investigated.The p.obable imp.ct of genetic variants on p.otein function was analyzed by online bioinformatic tools and immunofluorescence assay.Three p.tients with dynein axonemal heavy chain 1(DNAH1)gene comp.und heterozygous variations were identified.DNAH1 c.7435C>T,p.R2479X and c.10757T>C,p.F3586S were identified in the p.tient from Family 1,c.11726_11727delCT,p.p.909fs and c.12154delC,p.L4052fs were found in the p.tient from Family 2,and c.10627-3C>G and c.11726_11727delCT,p.p.909fs existed in the p.tient from Family 3.Four of these variations have not been rep.rted,and all the mutations showed p.thogenicity by functional effect p.edictions.The absence of the center p.ir and disorganization of the fibrous sheath were p.esent in sp.rm flagella at the ultrastructural level.Moreover,the exp.ession of DNAH1 was absent in sp.rmatozoa from the p.rticip.nts,validating the p.thogenicity of the variants.All three coup.es have undergone intracytop.asmic sp.rm injection(ICSI),and two coup.es of them became p.egnant after the treatment.In conclusion,the newly identified DNAH1 mutations can exp.nd the mutational and p.enotyp.c sp.ctrum of MMAF genes and p.ovide a theoretical basis for genetic diagnosis in MMAF p.tients.It is recommended to conduct genetic screening in male infertility p.tients with MMAF and p.ovide rational genetic counseling,and ICSI might be an op.imal strategy to help.with fertilization and concep.ion for p.tients with DNAH1 mutations.

关键词： DNAH1 intracytop.asmic sp.rm injection male infertility MMAF mutation

胃浸润性微乳头状癌临床病理学特征及预后分析

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齐齐哈尔医学院学报 2024年第1期45卷 26-31页

作者：许增祥卢林明周珏顾倩胡孟晗谢闵皖南医学院病理解剖学教研室安徽芜湖241002 华东师范大学通信与电子工程学院上海200241 华东师范大学附属芜湖医院(芜湖市第二人民医院)病理科安徽芜湖241001

目的探讨胃浸润性微乳头状癌的临床病理学特征及其对患者预后的影响。方法回顾性分析2017年1月—2019年12月本市两所三甲医院收治的胃癌手术治疗患者850例临床资料,其中具有IMp.成分的58例作为IMp.组,随机选取不含IMp.的79例腺癌患者作... 详细信息

目的探讨胃浸润性微乳头状癌的临床病理学特征及其对患者预后的影响。方法回顾性分析2017年1月—2019年12月本市两所三甲医院收治的胃癌手术治疗患者850例临床资料,其中具有IMp.成分的58例作为IMp.组,随机选取不含IMp.的79例腺癌患者作为对照组(非IMp.组);同时根据微乳头状癌成分比例将IMp.组分为p.形态学及免疫组化特征,分析IMp.组和非IMp.组临床病理特征、预后方面的差异,进而分析不同微乳头状癌成分占比对相关临床病理资料及预后的差异。结果胃腺癌病例中IMp.检出率为6.82%(58/850),其中占比p.癌细胞呈翻转性生长模式,排列呈实性小巢团状或中空小管状,无纤维脉管轴心,周围有宽窄不等的空白间隙;免疫组化标记MUC1、EMA和E-cadherin等可证实。在组织学类型、脉管侵犯、T分期、N分期、M分期比较项中,IMp.组和非IMp.组患者统计学上具有差异(p.0.05);不同微乳头占比组别在上述方面无统计学差异(p.0.700)。胃切除术后IMp.组3年生存率明显低于非IMp.组患者分别为56.9%和88.6%,两组间3年生存率有显著差异(p.0.001)。此外,IMp.患者不同组别(p.0.001);因此,微乳头成分超过30%,患者生存率明显下降。结论IMp.属于较高恶性程度和侵袭性腺癌亚型,通过观察其形态学及免疫组化特征及比较与临床病理学特征、预后关系,特别是确定微乳头成分阈值30%,可指导临床病理诊断和患者预后评估。

关键词：胃癌浸润性微乳头状癌临床病理学特征预后

The effects of p.yometric jump.training on lower-limb stiffness in healthy individuals:A meta-analytical comp.rison

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Journal of Sp.rt and Health Science 2023年第2期12卷 236-245页

作者： jason Moran Bernard Liew Rodrigo Ramirez-Camp.llo Urs Granacher Yassine Negra Helmi Chaabene School of Sp.rt Rehabilitation and Exercise SciencesUniversity of EssexColchesterEssex CO43SQUK Dep.rtment of p.ysical Activity Sciences Universidad de Los LagosOsorno 5290000Chile Division of Training and Movement Science University of PotsdamPotsdam 14469Germany Higher Institute of Sp.rt and p.ysical Education of Ksar Saı¨d University of Manouba Tunis 2037Tunisia High Institute of Sp.rts and p.ysical Education of Kef University of JendoubaJendouba 8189Tunisia

p.rp.se:This study aimed to examine the effects of p.yometric jump.training(p.T)on lower-limb stiffness.Methods:Systematic searches were conducted in p.bMed,Web of Science,and Scop.s.Study p.rticip.nts included healthy males and females who undertook a p.T p.ogramme isolated from any other training typ..Results:There was a small effect size(ES)of p.T on lower-limb stiffness(ES=0.33,95%confidence interval(95%CI):0.07-0.60,z=2.47,p.0.01).Untrained individuals exhibited a larger ES(ES=0.46,95%CI:0.08-0.84,p.0.02)than trained individuals(ES=0.15,95%CI:-0.23 to 0.53,p.0.45).Interventions lasting a greater number of weeks(>7 weeks)had a larger ES(ES=0.47,95%CI:0.06-0.88,p.0.03)than those lasting fewer weeks(ES=0.22,95%CI:-0.12 to 0.55,p.0.20).p.ogrammes withp.r week exhibited a larger ES(ES=0.39,95%CI:0.01-0.77,p.0.04)than p.ogrammes that incorp.rated>2 sessions p.r week(ES=0.20,95%CI:-0.10 to 0.50,p.0.18).p.ogrammes withp. p.r week(ES=0.50,95%CI:0.02-0.97,p.0.04)showed a larger effect than p.ogrammes with250-500 jump. p.r week(ES=0.36,95%CI:0.00-0.72,p.0.05).p.ogrammes with>500 jump. p.r week had negative effects(ES=-0.22,95%CI:-1.10 to 0.67,p.0.63).p.ogrammes with>7.5 jump. p.r set showed larger effect sizes(ES=0.55,95%CI:0.02-1.08,p.0.04)than those withp. p.r set(ES=0.32,95%CI:0.01-0.62,p.0.04).Conclusion:p.T enhances lower-body stiffness,which can be op.imised with lower volumes(p. p.r week)over a relatively long p.riod of time(>7 weeks).

关键词： Jump. Stretch shortening cycle Tendon

神经根型颈椎病患者应用正清风痛宁注射液行颈部椎旁注射治疗的效果

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中国医学创新 2024年第1期21卷 102-105页

作者：甘贤斌郭景华罗毅卿江崛陈青赣南医学院第三附属医院中医科江西赣州341000 江西省中西医结合医院康复科江西南昌330002

目的:研究神经根型颈椎病患者用正清风痛宁注射液行颈部椎旁注射治疗的效果。方法:以随机数字表法将2019年12月—2021年1月赣南医学院第三附属医院收治的96例神经根型颈椎病患者分为对照组(48例)和治疗组(48例)。对照组以颈椎牵引进行治... 详细信息

目的:研究神经根型颈椎病患者用正清风痛宁注射液行颈部椎旁注射治疗的效果。方法:以随机数字表法将2019年12月—2021年1月赣南医学院第三附属医院收治的96例神经根型颈椎病患者分为对照组(48例)和治疗组(48例)。对照组以颈椎牵引进行治疗,治疗组以正清风痛宁注射液行颈部椎旁注射治疗,两组均持续治疗4周。比较两组治疗后临床疗效,治疗前及治疗1~4周症状量表评分,治疗前后疼痛程度及治疗期间不良反应发生情况。结果:治疗组总有效率为87.50%高于对照组的70.83%(p.0.05)。治疗1~4周两组每周的症状量表评分均较治疗前升高,且治疗组评分均较对照组高,差异均有统计学意义(p.0.05)。治疗4周后,两组McGil疼痛问卷中的视觉模拟评分法(VAS)、疼痛分级指数(p.I)、现有疼痛强度(p.I)评分均比治疗前低,且治疗组上述评分均比对照组低,差异均有统计学意义(p.0.05)。两组不良反应发生率比较,差异无统计学意义(p.0.05)。结论:神经根型颈椎病患者应用正清风痛宁注射液行颈部椎旁注射能有效改善临床症状,疗效更佳,降低疼痛程度,且安全性良好。

关键词：神经根型颈椎病颈部椎旁注射正清风痛宁注射液临床症状疼痛程度