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Content of blood cell components,inflammatory cytokines and growth factors in autologous platelet-rich plasma obtained by various methods

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World Journal of Orthopedics 2022年第6期13卷 587-602页

作者： Maciej Dejnek jarosław witkowski Helena Moreira Sylwia Płaczkowska Piotr Morasiewicz PawełReichert Aleksandra Królikowska Department of Trauma Surgery Wroclaw Medical UniversityWroclaw 50-556Poland Department of Medical Science Foundation Wroclaw Medical UniversityWroclaw 50-556Poland Teaching and Research Diagnostic Laboratory Department of Laboratory DiagnosticsWroclaw Medical UniversityWroclaw 50-556Poland Institute of Medical Sciences Department of Orthopaedic and Trauma SurgeryUniversity of OpoleOpole 45-052Poland Ergonomics and Biomedical Monitoring Laboratory Department of PhysiotherapyWroclaw Medical UniversityWroclaw 50-355Poland

BACKGROUND The evaluation of the efficacy of platelet-rich plasma(PRP) in clinical practice yields conflicting results and raises numerous controversies. This may be due to different concentrations of biologically active components in PRP obtained with the use of different methods of gravity *** To compare the content, repeatability and correlations between biologically active components in PRP obtained with four different commercial *** From a whole blood sample of each of 12 healthy male volunteers, 4 PRP samples were prepared using 4 different commercial kits [Arthrex Autologous Conditioned Plasma(ACP), Mini GPS Ⅲ, Xerthra, Dr. PRP] in accordance with the instructions provided by the manufacturers. A comparative analysis of blood cell components-13 selected inflammatory cytokines and 7 growth factors-in the obtained PRP samples was performed using the Kruskal-Wallis test by ranks. The repeatability of results in each method was evaluated by the estimation of the coefficient of variation. The Spearman correlation was used to estimate the relationship between blood cell content and *** Significantly higher concentrations of platelets(PLT), white blood cells(WBC) and red blood cells(RBC) were found in PRP obtained with the use of Mini GPS Ⅲ than in PRP obtained using other systems. Significant differences in the content of growth factors and cytokines in PRP were found. A positive correlation of the amount of PLT, RBC and WBC with the concentration of most of the growth factors was found but in only three inflammatory cytokines. The obtained correlations between blood cell components and cytokines differed between the systems in terms of statistical significance, which may be due to insufficient sample size. The repeatability of the obtained PLT concentration also varied between protocols with the lowest in Xerthra and the highest in Arthrex *** Significant differences in the content of biologically active components a

关键词： Platelet-rich plasma Cytokines Chemokines Growth factors

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Influence of Biomass Co-combustion on Heating Surfaces Thermal Efficiency

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Journal of Thermal Science 2018年第5期27卷 427-432页

作者： GRADZIEL Slawomir witkowski Karol Institute of Thermal Power Engineering Cracow University of Technology31-864 Kraków Poland European Institute for Energy Research 76131 Karlsruhe Germany

Pulverized coal-fired(PCF) boilers were first and foremost intended to fire pulverized hard or brown coal. However, biomass co-firing has become a fairly common practice in the Polish power generation system and many existing boilers have been modernized to serve this purpose. This paper presents calculations of the coefficient of thermal efficiency of the boiler heating surfaces and of the time needed for complete reconstruction of deposits on the second-stage steam reheater(RHII) of an OP-380 boiler with the output of 380×10~3 kg/h. The boiler was equipped with a purpose-designed installation of direct feeding of biomass. The main co-fired fuels were wood and sunflower husk pellets. Intense formation of deposits on the steam reheater tubes and problems related to a reduction in the diameters of the tubes were identified during the power unit operation.

关键词： biomass co-combustion ash deposit coefficient of thermal efficiency

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Reduction of Tb^4+ ions in luminescent Y2O3:Tb nanorods prepared by microwave hydrothermal method

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Journal of Rare Earths 2016年第8期34卷 774-781页

作者： jaroslaw Kaszewski Bartlomiej S. witkowski Lukasz Wachnicki Hanka Przybylinska Boleslaw Kozankiewicz Ewa Mijowska Marek Godlewski Institute of Physics Polish Academy of Sciences al. Lotnik6w 32/46 02-668 Warsaw Poland Institute of Chemical and Environment Engineering West Pomeranian University of Technology ul. Putaslego 10 70-322 Szczecin Poland

Terbium doped yttrium oxide was prepared with the microwave hydrothermal method. The Y2O3：Tb nanomaterial crystal- lized as needle-like grains. Bright luminescence in the green region was observed. Significant luminescence intensity increase was obtained after thermal treatment. Reduction of terbium ions was observed after heating in the air atmosphere. Tb4＋ ions were found to be stabilized by crystal impurities. Hydroxyl species were found to have impact on vacancies elimination. The terbium ions were used as optical and magnetic indicator of the material properties.

关键词： yttrium oxide terbium luminescence biomarkers abnormal reduction rare earths

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Chinia ***.—the second diatom genus simonsenioid raphe from mangroves in Fujian,China

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Journal of Oceanology and Limnology 2022年第3期40卷 1220-1232页

作者： Huina LIN Weiwei WU Lin SUN Andrzej witkowski Xiaoye LI Vishal PATIL Junrong LIANG Xuesong LI Yahui GAO Changping CHEN School of Life Sciences and Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems Xiamen UniversityXiamen 361102China State Key Laboratory of Marine Environmental Science Xiamen UniversityXiamen 361102China Institute of Marine and Environmental Sciences University of SzczecinMickiewicza 16aPL-70-383 SzczecinPoland

A new monotypic diatom genus Chinia *** estuarine mangrove habitats in Fujian,China is ***,scanning and transmission electron microscopy observations of its morphology show that this diatom differs from any established raphid diatom *** new genus is distinguished by a strongly undulate valve face,eccentric raphe canal,and perforated *** distinctly elevated and perforated raphe canal,classifi ed as simonsenioid type,is supported by sparse infundibulum-like portulae,and the portulae are separated by large *** valves of Chinia also have hymenate areolar occlusions and the raphe lacks a central ***,unlike most Bacillariaceae with two plastids,including Simonsenia,Chinia has a single plastid similar to those in Entomoneis and *** the similarities to Simonsenia,Chinia features porous raphe canal and loose striation,which is different from those of Simonsenia that has a solid raphe canal and distinct rows of *** distinctive cytological and morphological characters suggest an affinity of the new genus to the Bacillariaceae.

关键词： marine diatoms East China Sea taxonomy Bacillariaceae new genus simonsenioid raphe

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HfO2:Eu nanoparticles excited by X-rays and UV-visible radiation used in biological imaging

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Journal of Rare Earths 2019年第11期37卷 1176-1182页

作者： jaroslaw Kaszewski jaroslaw Olszewski Julita Rosowska Bartlomiej witkowski Lukasz Wachnicki Karolina Wenelska Ewa Mijowska Zdzislaw Gajewski Marek Godlewski Michal M.Godlewski Institute of Physics Polish Academy of SciencesAl.Lotnikow 32/4602-668 Warsaw.Poland Veterinary Research Centre Department of Large Animal Diseases with ClinicFaculty of Veterinary MedicineWarsaw University of Life Sciences一SGGVK Nowoursynowska 10002-797 WarsawPoland Department of Physiological Sciences Faculty of Veterinary MedicineWarsaw University of Life Sciences-SGGWNowoursynowska 15902-776 WarsawPoland Faculty of Chemical Technology and Engineering West Pomeranian University of Technology in Szczecinal.Piastow 4570-311 SzczecinPoland

Europium doped hafnium dioxide nanoparticles were crystallized using biologically friendly microwave hydrothermal method and employed in observation of small rodents *** solvent in synthesis is water,in consequence nanoparticles are found surrounded by thin hydroxide *** of the nanoparticles is controlled by temperature of calcination.1200℃heating causes drop of luminescence intensity,explained as damage of high symmetry surface emission centers in the *** the other hand,growth of crystallites increases intensity of X-ray and electron beam excited *** makes them promising activator in photodynamic therapy.

关键词： HfO2 Europium Scintillators Hydrothermal Nanoparticles Rare earths

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Shape control over microwave hydrothermally grown Y2O3:Eu by europium concentration adjustment

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Journal of Rare Earths 2019年第11期37卷 1206-1212页

作者： jaroslaw Kaszewski Julita Rosowska Bartlomiej S.witkowski Lukasz Wachnicki Karolina Wenelska Ewa Mijowska Lev-Ivan Bulyk Damian Wlodarczyk Andrzej Suchocki Boleslaw Kozankiewicz Marek Godlewski Institute of Physics Polish Academy of SciencesWarsawLotnikow 32/4602-668Poland Institute of Chemical and Environment Engineering West Pomeranian University of TechnologySzczecinPulaskiego 1070-322SzczecinPoland

Two-step synthesis of Y2O3:Eu nanostructures was *** includes microwave driven hydrothermal and calcination *** route results in crystallization of Y4O(OH)g(NO3):Eu crystals initially,then Y2O3:Eu crystals after *** Eu contents in relation to overall cation quantity were set to 2 mol%,10 mol%and 20 mol%.Varying europium concentrations influence habit of obtained Y4O(OH)g(NO3):Eu crystals from needle-like to plate-like and as a result,also shapes of final Y2O3:Eu ***,certain amount of Eu2+ions was detected in as-grown material using laser spectroscopy and decay kinetics *** material was calcined at 1200°C in the air,which results in oxidation of Eu2+ions and crystallization of small number of cubic EU2 O3 *** of obtained materials was performed using XRD,SEM,TEM,EDX,CL,Raman and photoluminescence spectroscopy.

关键词： Yttrium oxide Europium Luminescence Hydrothermal Microwave Rare earths

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肿瘤研究历史回顾(1900～1990)

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国外医学（肿瘤学分册） 1991年第4期18卷 193-202页

作者： witkowski J A 刘培楠

在20世纪,肿瘤是许多发达国家的主要死亡原因。因此肿瘤研究在本世纪中成为生物医学研究发展的重要推动力。一般深信对机体的基本过程的阐明将有助于了解正常细胞成为恶性的机制,所以这项研究并未只限于以人类肿瘤为对象,而且采用动物... 详细信息

在20世纪,肿瘤是许多发达国家的主要死亡原因。因此肿瘤研究在本世纪中成为生物医学研究发展的重要推动力。一般深信对机体的基本过程的阐明将有助于了解正常细胞成为恶性的机制,所以这项研究并未只限于以人类肿瘤为对象,而且采用动物实验开展了各学科的研究,包括生物化学、遗传学、分子生物学、细胞生物学、发育生物学和免疫学等各领域的探讨。

关键词：肿瘤研究历史

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1987年股市崩盘的10条教训

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中国证券期货 2012年第11期15卷 78-80页

作者： Wallace witkowski

25年前，也就是1987年10月19日，道琼斯工业平均指数（DowJonesIndustrialAverage）暴跌近23％，这是该指数有史以来最高的单日跌幅。尽管这次暴跌没有引发另一场大萧条，但它的确使投资者进入了股市波动的新时代。

关键词：股市崩盘道琼斯工业平均指数股市波动大萧条投资者暴跌跌幅

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分子生物学—五十年的光辉历程(续)

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生物技术通报 1989年第7期5卷 1-6页

作者： Jan witkowski 朱遐

1965 揭示琥珀和赭石突变基因分别是链终止密码子 UAG 和 UAA。Weigert,M.G.和 Garen,A.由碱性磷酸酶作用下色氨酸位点的氨基酸的置换证明大肠杆菌中无意义密码子的碱基组成。Nature 206,992～994Brenner,S.,Stretton,A.O.和 Kaplan,S.... 详细信息

1965 揭示琥珀和赭石突变基因分别是链终止密码子 UAG 和 UAA。Weigert,M.G.和 Garen,A.由碱性磷酸酶作用下色氨酸位点的氨基酸的置换证明大肠杆菌中无意义密码子的碱基组成。Nature 206,992～994Brenner,S.,Stretton,A.O.和 Kaplan,S.遗传密码:链终止"无意义"三联体密码及其抑制。Nature 206。

关键词：无意义密码子赭石突变三联体密码链终止密码子碱基组成 Brenner 限制性位点碱性磷酸酶遗传密码遗传操作

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Duchenne肌营养不良症的分子遗传学

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国外医学（遗传学分册） 1989年第2期12卷 77-79页

作者： witkowski JA 孙艳阳

分子生物学,特别是重组DNA技术的出现,使我们对人类遗传病有了更深入的认识。这一领域的进展是极其迅速的,其中最明显的例子就是对X-连锁遗传病Duchenne肌营养不良症(DMD)的研究。用基因组探针检测携带者和产前诊断已经证明XJ1.1和pERT... 详细信息

分子生物学,特别是重组DNA技术的出现,使我们对人类遗传病有了更深入的认识。这一领域的进展是极其迅速的,其中最明显的例子就是对X-连锁遗传病Duchenne肌营养不良症(DMD)的研究。用基因组探针检测携带者和产前诊断已经证明XJ1.1和pERT基因组探针,及侧翼探针如C7、754和99.6对于发现DMD携带者和产前诊断具有不可估量的价值。受累患儿血清CK水平是正常儿童的100～200倍,但用于检测携带者却不可靠,因为有1/3的肯定携带者的CK水平在正常范围。

关键词：肌营养不良症分子遗传学

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