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Journal of Huazhong University of Science and Technology(Medical Sciences) 2012年第2期32卷 299-302页

作者：王辰允王颖莹王淼陈建奎于农宋世平 Norbert E.kaminski 张伟 Department of Pharmaceutical Administration PLA General Hospital Outpatient Department of Xiyuan PLA General Hospital Department of Clinical Laboratory No. 307 Hospital Academy of Military Medical Sciences Department of Pharmacology and Toxicology and the Center for Integrative Toxicology Michigan State University East Lansing

Many researchers employed mammalian expression system to artificially express cannabinoid receptors, but immunoblot data that directly prove efficient protein expression can hardly be seen in related research reports. In present study, we demonstrated cannabinoid receptor protein was not able to be properly expressed with routine mammalian expression system. This inefficient expression was rescued by endowing an exogenous signal peptide ahead of cannabinoid receptor peptide. In addition, the artificially synthesized cannabinoid receptor was found to aggregate under routine sample denaturing temperatures (i.e.,≥95°C), forming a large molecular weight band when analyzed by immuno-blotting. Only denaturing temperatures ≤75°C yielded a clear band at the predicted molecular weight. Collectively, we showed that efficient mammalian expression of cannabinoid receptors need a signal peptide sequence, and described the requirement for a low sample denaturing temperature in immuno-blot analysis. These findings provide very useful information for efficient mammalian expression and immuno-blotting of membrane receptors.

关键词： cannabinoid receptor 1 cannabinoid receptor 2 denaturing temperature signal peptide mammalian expression

纳秒脉冲在普通单模光纤中产生超连续谱的实验研究

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中国激光 2011年第7期38卷 162-166页

作者：刘楚 Eric Rees Toni Laurila 简水生 Clemens kaminski 北京交通大学全光网络与现代通信网教育部重点实验室北京100044 北京交通大学光波技术研究所北京100044 Depart ment of Chemical Engineering and Biotechnology University of CambridgePembroke StreetCB2 3RAUK

利用中心波长1064 nm的纳秒激光脉冲入射到普通单模光纤中,获得了从700 nm到超过1750 nm的超连续谱输出。在正常色散区抽运下,光谱首先出现多级拉曼斯托克斯线,随着抽运功率或光纤长度的增加,斯托克斯能量将进入光纤反常色散区,形成光孤... 详细信息

利用中心波长1064 nm的纳秒激光脉冲入射到普通单模光纤中,获得了从700 nm到超过1750 nm的超连续谱输出。在正常色散区抽运下,光谱首先出现多级拉曼斯托克斯线,随着抽运功率或光纤长度的增加,斯托克斯能量将进入光纤反常色散区,形成光孤子,随后与孤子相关的非线性效应将使光谱进一步展宽。实验结果表明,当单模光纤长度为28 m时,产生的光谱在1260~1750 nm范围内,有较好的光谱平坦度（小于3.8 dB）、较强的光谱光功率密度（平均约为0.4 mW/nm）并且单模输出。

关键词：非线性光学纳秒脉冲普通单模光纤超连续谱

Converting lateral scanning into axial focusing to speed up three-dimensional microscopy

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Light(Science & Applications) 2020年第1期9卷 429-440页

作者： Tonmoy Chakraborty Bingying Chen Stephan Daetwyler Bo-Jui Chang Oliver Vanderpoorten Etai Sapoznik Clemens F.kaminski Tuomas P.J.Knowles Kevin M.Dean Reto Fiolka Department of Cell Biology UT Southwestern Medical CenterDallasTXUSA Department of Physics and Astronomy University of New MexicoAlbuquerqueNMUSA Department of Chemical Engineering and Biotechnology University of CambridgeCambridge CB30ASUK Department of Chemistry University of CambridgeLensfield RoadCambridge CB21EWUK Lyda Hill Department of Bioinformatics UT Southwestern Medical CenterDallasTXUSA

In optical microscopy,the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric ***,by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens(ETL)to the back focal plane,rapid axial scanning has been ***,mechanical actuation of a mirror limits the axial scanning rate(usually only 10–100 Hz for piezoelectric or voice coil-based actuators),while ETLs introduce spherical and higher-order aberrations that prevent high-resolution *** an effort to overcome these limitations,we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution *** a galvanometric mirror,we scan a laser beam laterally in a remote-focusing arm,which is then back-reflected from different heights of a mirror in the image *** characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude,allowing the quantification of rapid vesicular dynamics in three *** also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope,which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution.

关键词： mirror axial image

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《鬼》试译研究报告

《鬼》试译研究报告

作者： Jannis Paul kaminski 北京外国语大学

学位级别：硕士

为了获知目的论应用在文学作品中的新见解,笔者选择了“巴金”的短篇小说《鬼》进行翻译,分析翻译时遇到的问题并定义译者作用。巴金是中国最知名的作家之一。他于1934年—1935年间在日本游历,受其所见所闻的启发,他写了三部曲《神,鬼,... 详细信息

为了获知目的论应用在文学作品中的新见解,笔者选择了“巴金”的短篇小说《鬼》进行翻译,分析翻译时遇到的问题并定义译者作用。巴金是中国最知名的作家之一。他于1934年—1935年间在日本游历,受其所见所闻的启发,他写了三部曲《神,鬼,人》。三部曲的整体内容可以理解为呼吁反对虔诚和宗教神权。第二部短篇小说《鬼》是笔者试翻译的主题。在这个故事中,作者描述了一位在学生时代就已表现出极大虔诚的日本老师的变化过程。因相思,他更寄托于宗教的支持。他坚信超自然世界和鬼的存在。在笔者的论文中,笔者简要介绍了作者的作品并分析了作品的时代背景。其次,笔者阐释了对翻译学及文学翻译的见解。关于理论基础,笔者得出的结论是:翻译行动理论的一部分为目的论,受到忠实原文原则和功能充分性原则的影响,为短篇小说的翻译提供了良好的基础。同样,文学译者的发挥也受到源文本作者所提供的框架所限制。译者处理源文本的内容和作者的意图时,会识别此框架。在分析具体翻译问题前,笔者先定义翻译假设和翻译目标,然后进行翻译分析。翻译的目标是使翻译尽量贴近字面意思(符合原著的创作时间),尽量贴合德语的表述方式。译本的潜在目标人群是对文学感兴趣、不懂中文、不是很了解日本和中国文化的德国读者。首先,笔者为翻译问题的解决找到相应的翻译方法,并解释了每种翻译决定的原因。最后,笔者总结了写论文的过程和结果如下:在分析了不同类别的翻译问题并解释了笔者使用的翻译方法之后,笔者意识到译者在文学翻译中享有极大的创作自由。笔者在文学翻译中运用受忠实原文原则和功能充分性原则影响的目的论,并发挥了文化传介人的作用。根据笔者对目标人群对中国和日本文化的了解程度的假设,笔者已对翻译进行了适当改编,以使目标读者没有任何理解上的问题。但是,与此同时,笔者也为德国读者保留了这部短篇小说中的文化差异。

关键词：巴金鬼文学翻译目的论

CXCR4 down-regulation by small interfering RNA inhibits invasion and tubule formation of human retinal microvascular endothelial cells

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CXCR4 down-regulation by small interfering RNA inhibits inva...

Lumacaftor/ivacaftor therapy is associated with reduced hepatic steatosis in cystic fibrosis patients

中华医学会第十二届全国眼科学术大会

作者： kaminski JM Ambati B State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060PR China

The progressive alterations to the retinal microvasculature in diabeticretinopathy are known to cause vision ***- kines are characterized by their ability to induce cell invasion, adhesion and *** this study,we used d...

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World Journal of Hepatology 2019年第12期11卷 761-772页

作者： Katherine Kutney Shannon B Donnola Chris A Flask Rose Gubitosi-Klug MaryAnn O’Riordan Kimberly McBennett Thomas J Sferra Beth kaminski Department of Pediatric Endocrinology University Hospitals Cleveland Medical CenterClevelandOH 44106United States Department of Pediatrics Case Western Reserve UniversityClevelandOH 44106United States Department of Radiology Case Western Reserve University ClevelandOH 44106United States Department of Biomedical Engineering Case Western Reserve UniversityClevelandOH 44106United States

BACKGROUND Hepatic steatosis is a common form of cystic fibrosis associated liver disease(CFLD)seen in an estimated 15%-60%of patients with cystic fibrosis(CF).The pathophysiology and health implications of hepatic steatosis in cystic fibrosis remain largely *** the general population,hepatic steatosis is strongly associated with insulin resistance and type 2 *** fibrosis related diabetes(CFRD)impacts 40%-50%of CF adults and is characterized by both insulin insufficiency and insulin *** hypothesized that patients with CFRD would have higher levels of hepatic steatosis than cystic fibrosis patients without *** To determine whether CFRD is associated with hepatic steatosis and to explore the impact of lumacaftor/ivacaftor therapy on hepatic steatosis in *** Thirty patients with CF were recruited from a tertiary care medical center for this cross-sectional *** pancreatic insufficient patients with CFRD or normal glucose tolerance(NGT)were *** with established CFLD,end stage lung disease,or persistently elevated liver enzymes were *** magnetic resonance imaging(MRI)proton density fat fraction(PDFF)was obtained for all *** characteristics[age,sex,body mass index,percent predicted forced expiratory volume at 1 s(FEV1),lumacaftor/ivacaftor use]and blood chemistries were assessed for possible association with hepatic *** steatosis was defined as a mean MRI PDFF>5%.Patients were grouped by diabetes status(CFRD,NGT)and cystic fibrosis transmembrane conductance regulator(CFTR)modulator use(lumacaftor/ivacaftor,no lumacaftor/ivacaftor)to determine between group *** variables were analyzed with a Wilcoxon rank sum test and discrete variables with a Chi square test or Fisher’s exact *** Twenty subjects were included in the final *** median age was 22.3 years(11.3-39.0)and median FEV1 was 77%(33%-105%).Twelve subjects had CFRD and 8 had N

关键词： Cystic fibrosis Liver disease Non-alcoholic fatty liver disease Cystic fibrosis transmembrane conductance regulator Lumacaftor/ivacaftor Cystic fibrosis transmembrane conductance regulator modulator Diabetes mellitus

Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assemblies

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Journal of Molecular Cell Biology 2021年第4期13卷 282-294页

作者： Maarten C.Hardenberg Tessa Sinnige Sam Casford Samuel T.Dada Chetan Poudel Elizabeth A.Robinson Monika Fuxreiter Clemens F.Kaminksi Gabriele S.kaminski Schierle Ellen A.A.Nollen Christopher M.Dobson Michele Vendruscolo Centre for Misfolding Diseases Department of ChemistryUniversity of CambridgeCambridge CB21EWUK Department of Chemical Engineering and Biotechnology University of CambridgeCambridge CB3 OASUK MTA-DE Laboratory of Protein Dynamics Department of Biochemistry and Molecular BiologyUniversity of DebrecenHungary European Research Institute for the Biology of Aging University Medical Center GroningenUniversity of GroningenGroningen 9713 AVThe Netherlands Present address Bijvoet Centre for Biomolecular Research Department of ChemistryUtrecht UniversityUtrecht 3584 CHThe Netherlands

Misfoldedα-synudein is a major component of Lewy bodies,which are a hallmark of Parkinson's disease(PD).A large body of evidence shows thatα-synuclein can aggregate into amyloid fibrils,but the relationship between a-synuclein self-assembly and Lewy body formation remains ***,we show,both in vitro and in a Caenorhabditis elegans model of PD,thatα-synuclein undergoes liquid-liquid phase separation by forming a liquid droplet state,which converts into an amyloid-rich hydrogel with Lewy-body-like *** maturation process towards the amyloid state is delayed in the presence of model synaptic vesicles in *** together,these results suggest that the formation of Lewy bodies may be linked to the arrested maturation ofα-synuclein condensates in the presence of lipids and other cellular components.

关键词： α-synuclein,liquid-liquid phase separation,Parkinson's disease

INFLUENCE OF LOCAL LASER HARDENING ON STRUCTURE AND WEAR RESISTANCE OF CARBON STEELS

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INFLUENCE OF LOCAL LASER HARDENING ON STRUCTURE AND WEAR RES...

第三届摩擦疲劳学国际会议

作者： Vasyl Pokhmurskii Anna Pokhmurska Volodymyr Dovhunyk Orest kaminski Physico-Mechanical Institute of National Academy of Sciences of Ukraine 5 Naukova Str. 79601 LvivUkraine Institute for Applied Problems of Mechanics and Mathematics National Academy of Sciences of Ukraine 3 b Naukova Str. 79601 LvivUkraine Physico-Mechanical Institute of National Academy of Sciences of Ukraine 5 Naukova Str. 79601 LvivUkraine Physico-Mechanical Institute of National Academy of Sciences of Ukraine 5 Naukova Str. 79601 LvivUkraine

＜正＞Wear resistance of the middle-carbon （0.45%C） and high-carbon （0,8%C） steels has been investigated. Specimens were made in a form of rings with 10 mm width and 40 mm in diameter. Laser hardening of the external cylindrical surfaces has been carried out using CO2 2,5 kW continuous wave laser. Different schemes of laser treatment were used to create the hardened zones on the sample surface. Changing of laser passes overlapping degree and the direction of laser beam scanning varied shape of hardened areas. Block-on-ring sliding test was carried out for tribological investigations. The second element of the friction couple - block was processed from bearing steel （HRC 57）. Normal loading was 5 MPa, sliding velocity of the specimen - 0,67 m/s. Abrasive-oil mixture was used for lubrication. The sliding distance was 20 km. Structure heterogeneity and microhardness distribution have been investigated. It was shown that the wear resistance of large surfaces could be increased by laser treatment. The wear rate of these surfaces is influenced by the structural conditions, laser processing parameters and the shape and location of treated zones.

关键词： CW CO2 laser beam treatment surface hardening eutectoid steel microhardness wear friction coefficient.

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灵活的起重机解决方案

现代制造 2009年第37期 42-43页

作者： MICHAEL kaminski

钢铁加工企业Tecade集团为其新建的生产车间和物料仓库订购了一套Demag Cranes & Components公司的标准起重设备。除了采用双主梁桥式起重机和半门式起重机之外,Demag还为许多工作岗位配备了悬臂起重机,可以在车间上方灵活地应用。

关键词：门式起重机 Components公司生产车间桥式起重机加工企业起重设备工作岗位

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2型大麻受体的克隆及真核表达体系的构建

第三军医大学学报 2007年第11期29卷 1003-1005页

作者：王颖莹陈纯海裴莉萍张伟 Norbert E.kaminski 第三军医大学西南医院检验科重庆400038 第三军医大学军事预防医学院劳动卫生学教研室重庆400038 美国密西根州立大学药理与毒理系东兰辛48824

目的克隆人2型大麻受体(hCB2)基因,并构建相应的稳定表达细胞株。方法利用人T淋巴细胞株HPB-ALL的总RNA,以RT-PCR及酶切、连接等分子生物学方法克隆hCB2基因于质粒载体pFlag-CMV-3内,以HEK293细胞构建稳定表达细胞株,以Western blot方... 详细信息

目的克隆人2型大麻受体(hCB2)基因,并构建相应的稳定表达细胞株。方法利用人T淋巴细胞株HPB-ALL的总RNA,以RT-PCR及酶切、连接等分子生物学方法克隆hCB2基因于质粒载体pFlag-CMV-3内,以HEK293细胞构建稳定表达细胞株,以Western blot方法利用蛋白标记Flag的单克隆抗体(Anti-Flag)和CB2多克隆抗体(Anti-CB2)同时检测质粒的表达。结果酶切及测序结果表明hCB2基因克隆成功,Western blot检测结果表明Anti-Flag和Anti-CB2都能检测Flag-hCB2蛋白。结论成功克隆hCB2基因,并获得稳定表达细胞株,同时证实Anti-CB2多克隆抗体有效。

关键词： 2型大麻受体基因克隆真核表达