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Development of Standard Reagents for Avian Influenza Virus Subtypes Diagnosis

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Journal of Agricultural Science and Technology(A) 2019年第1期9卷 26-37页

作者： Maria Angela Orsi Soraya cecilia Albieri camillo Eluana carolina Fortunato christian Steffe Domingues Dilmara Reischak Margarida Maria Hoppner Zaroni Tania Rosária Pereira Freitas Terra A.Jenson janice c.pedersen Agriculture and Livestock National Laboratory-Sao Paulo LANAGRO-SPMinistry of AgricultureLivestock and SupplayMAPACampinasSao PauloBrazil Agriculture and Livestock National Laboratory-Minas Gerais LANAGRO-MGMinistry of AgricultureLivestock and SupplayMAPAPedro LeopoldoMinas GeraisBrazil U.S.Department of Agriculture Animal and Plant Health Inspection ServiceVeterinary ServicesNational Veterinary Services Laboratories(NVSL)AmesIAUSA

Avian influenza(AI)is one of the most relevant viruses in the poultry *** AI virus(AIV)transmission from birds to human causing severe cases and mortality enhanced the magnitude of AI for public ***,the AIV diagnosis laboratories should be able to detect and identify endemic,epidemic and seasonal influenza strains and other wildlife influenza subtypes that cross the country’s *** development in quality controls in according with international rules comes to improve the performance of *** this purpose,the Brazilian Reference Laboratory for Avian Influenza(LANAGRO-SP)established a cooperation with the World Organization for Animal Health(OIE)to produce AIV master seeds,inactivated antigens and antiserum to attend the necessities of Brazil and other South America countries under the high quality control for all *** of AIV master seed lots and seventeen of inactivated antigens lots produced reached hemagglutination(HA)titers of 1:512 and 1:256,*** addition,fifteen AIV antiserum lots with hemagglutination inhibition(HI)titers reaching 1:4,096 were *** AIV reference reagents produced and applied in laboratory routine successfully.

关键词： Avian influenza virus reference reagents subtypes quality control Brazil

Epidemiological, clinical Progress Aspects of congenital Heart Disease with Neonatal Revelation at the Mother-child Hospital of Bingerville (HME) concerning 98 cases from January 2021 to December 2022 (côte d’Ivoire)

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Open Journal of Pediatrics 2024年第1期14卷 89-100页

作者： Adja Evelyne Akaffou-Gbery Richard Azagoh-Kouadio Anne-Lise Laetitia Mobio Jean-Jacques Yao Atteby Serenah Marie janice N’vrah Anoh Kouassi Raoul Yao Department of Paediatrics and Neonatology H&#244pital Mère-Enfant de Bingerville (HME) Bingerville C&#244te d’Ivoire Department of Medical Pediatrics and Specialties CHU de Angré Abidjan C&#244te d’Ivoire

Introduction The distribution of congenital heart disease (cHD) in sub-Saharan Africa is highly imprecise and varies from one region to another due to the inequality of diagnostic facilities. The aim of this study was to determine the in-hospital prevalence of congenital heart disease in children at the Mother-child hospital of Bingerville (HME) by specifying the diagnostic, therapeutic and evolutionary aspects. Materials and methods We conducted a retrospective, descriptive, cross-sectional study at HME of Bingerville from January 2021 to December 2022. All newborns with congenital heart disease confirmed by echocardiography were included in the study. Results Of 656 admissions to the neonatology department over the study period, congenital heart disease accounted for 14.9% (98/656) of cases. In our series, 76.7% were diagnosed before the 1st week of life, with a mean chronological age of 5.18 days and extremes of 0 and 46 days. There were as many male patients (50%) as female (50%), i.e. a sex ratio of 1. These newborns were premature in 60.2% of cases, with a mean and median gestational age of 34 weeks’ amenorrhea. Most were left-right shunts (90.8%). Persistent ductus arteriosus (PDA) (48.9%) predominated, followed by atrial septal defect (38.7%), ventricular septal defect (13.3%), common trunk artery (cTA) (3.1%) and open septal pulmonary atresia (OSPA) (1%) as the primary cyanogenic heart disease. Pulmonary arterial hypertension (PAH) (50%) was primary in 38.8% and secondary (61.2%). The mortality rate was 30.6%, and all cTA patients died (100%), with a significant statistical relationship (p = 0.027). Progression under treatment was marked by clinical stabilization (68/98) in 69.4% of cases. conclusion: congenital heart disease is relatively common at the Bingerville HME. Access to echocardiography should be facilitated in neonatology departments for rapid diagnosis and optimal management of congenital heart disease in newborns.

关键词： Newborn congenital Heart Disease côte d’Ivoire

Mid-infrared upconversion imaging using femtosecond pulses

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Photonics Research 2019年第7期7卷 98-106页

作者： Ashik A.S. callum F.O'Donnell S.chaitanya Kumar M.Ebrahim-Zadeh P.Tidemand-Lichtenberg c.pedersen DTU Fotonik Technical University of DenmarkDK-4000 RoskildeDenmark Radiantis Edifici RDITMediterranean Technology Park08860 CastelldefelsBarcelonaSpain IcFO-lnstitut de ciencies Fotoniques Barcelona Institute of Science and Technology08860 CastelldefelsBarcelonaSpain Institucio catalana de Recerca i Estudis Avan gats(IcREA) Passeig Lluis Companys 2308010 BarcelonaSpain

Mid-infrared(mid-IR) imaging and spectroscopic techniques have been rapidly evolving in recent years, primarily due to a multitude of applications within diverse fields such as biomedical imaging, chemical sensing, and food quality inspection. Mid-IR upconversion detection is a promising tool for exploiting some of these applications. In this paper, various characteristics of mid-IR upconversion imaging in the femtosecond regime are investigated using a 4 f imaging setup. A fraction of the 100 fs, 80 MHz output from a Ti:sapphire laser is used to synchronously pump an optical parametric oscillator, generating 200 fs mid-IR pulses tunable across the 2.7–4.0 μm wavelength range. The signal-carrying mid-IR pulses are detected by upconversion with the remaining fraction of the original pump beam inside a bulk LiNbO3 crystal, generating an upconverted field in the visible/near-IR range, enabling silicon-based ccD detection. Using the same pump source for generation and detection ensures temporal overlap of pulses inside the nonlinear crystal used for upconversion, thus resulting in high conversion efficiency even in a single-pass configuration. A theory is developed to calculate relevant acceptance parameters, considering the large spectral bandwidths and the reduced interaction length due to group velocity mismatch, both associated with ultrashort pulses. Furthermore, the resolution of this ultrashort-pulsed upconversion imaging system is *** is demonstrated that the increase in acceptance bandwidth leads to increased blurring in the upconverted *** presented theory is consistent with experimental observations.

关键词： upconversion observations femtosecond regime

Sodium lauryl sulfate and its potential impacts on organisms and the environment:A thematic analysis

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Emerging contaminants 2023年第1期9卷 96-105页

作者： Johannes Reiner G.Asio janice S.Garcia charalabos Antonatos janice B.Sevilla-Nastor Lorele c.Trinidad School of Environmental Science and Management University of the Philippines-Los-Ba~nosCollegeLagunaPhilippines Laboratory of Genetics Department of BiologyUniversity of PatrasGreece National Institute of Molecular Biology and Biotechnology University of the Philippines-Los BanosCollegeLagunaPhilippines Planetary and Global Health Program St.Lukes Medical Center College of Medicine-William H Quasha MemorialQuezon CityPhilippines

Due to its ubiquitous presence in the environment and the lack of a scientific consensus regarding the environmental safety of the widely used anionic surfactant Sodium Lauryl Sulfate(cAS No:151-21-3),a systematic literature review and thematic analysis was *** studies about sodium lauryl sulfate(SLS)in the environment in key databases were reviewed,with coding methods used to identify impact categories from SLS exposure without potential narration *** on the limited number of studies on SLS,there is empirical evidence of this surfactant contributing to environmental toxicity at various concentrations(0.004-3509 mg L^(-1)),with aquatic organisms at a higher risk from ***,exposure to SLS can elicit changes to various organismic processes and environmental ***,further study on SLS in various environmental compartments is recommended to monitor the level of SLS pollution,understand its behavior upon contact with various environmental media,and understand its impacts on flora and ***,SLS quantification should be done on commonly used consumer products to potentially regulate its use and to consequently curb SLS pollution from its source.

关键词： SLS Environmental risk Thematic analysis Surfactant Environmental safety Emerging contaminant

In vitro 3D malignant melanoma model for the evaluation of hypericin-loaded oil-in-water microemulsion in photodynamic therapy

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Bio-Design and Manufacturing 2022年第4期5卷 660-673页

作者： Hui LMa Wanlu Li Mian Wang Laudemir c.Varanda janice R.Perussi Y.Shrike Zhang Emanuel carrilho Instituto de Química de São carlos Universidade de São PauloUSPAv.Trabalhador São-carlenseSão CarlosSP 13566-590Brazil Instituto Nacional de ciência e Tecnologia de Bioanalítica INCTBioCampinasSP 13083-970Brazil Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolCambridgeMA 02139USA

Advances in biomimetic three-dimensional(3D) melanoma models have brought new prospects of drug screening and disease modeling, since their physiological relevancy for recapitulating in vivo tumor architectures is more accurate than traditional two-dimensional(2D) cell culture. Gelatin methacryloyl(GelMA) is widely used as a tissue-engineered scaffold hydrogel for 3D cell culture. In the present study, an in vitro 3D malignant melanoma model based on Gel MA was fabricated to evaluate the efficiency of hypericin(Hy)-loaded microemulsion(ME) in photodynamic therapy against melanoma. The ME was produced by the spontaneous emulsification method to enhance the bioavailability of Hy at tumor sites. Hy-loaded MEs were applied to a 3D malignant melanoma model made using 6% Gel MA and the co-culture of B16F10 and Balb/c 3T3 cells,followed by crosslinking using violet light(403 nm). The observation revealed excellent cell viability and the presence of F-actin cytoskeleton network. Hy-loaded MEs exhibited higher phototoxicity and cell accumulation(about threefold) than free Hy, and the cells cultured in the 3D system displayed lower susceptibility(about 2.5-fold) than those in 2D *** findings indicate that the developed MEs are potential delivery carriers for Hy;furthermore, Gel MA hydrogel-based modeling in polydimethylsiloxane(PDMS) molds is a user-friendly and cost-effective in vitro platform to investigate drug penetration and provide a basis for evaluating nanocarrier efficiency for skin cancer and other skin-related diseases.

关键词： Malignant melanoma 3D cell culture Hypericin Microemulsion Photodynamic therapy

AdsorbML: a leap in efficiency for adsorption energy calculations using generalizable machine learning potentials

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npj computational Materials 2023年第1期9卷 565-573页

作者： janice Lan Aini Palizhati Muhammed Shuaibi Brandon M.Wood Brook Wander Abhishek Das Matt Uyttendaele c.Lawrence Zitnick Zachary W.Ulissi Fundamental AI Research(FAIR) Meta AIMetaMenlo ParkCAUSA Department of chemical Engineering Carnegie Mellon UniversityPittsburghPAUSA Scott Institute for Energy Innovation Carnegie Mellon UniversityPittsburghPAUSA

computational catalysis is playing an increasingly significant role in the design of catalysts across a wide range of applications.A common task for many computational methods is the need to accurately compute the adsorption energy for an adsorbate and a catalyst surface of ***,the identification of low-energy adsorbate-surface configurations relies on heuristic methods and researcher *** the desire to perform high-throughput screening increases,it becomes challenging to use heuristics and intuition *** this paper,we demonstrate machine learning potentials can be leveraged to identify low-energy adsorbate-surface configurations more accurately and *** algorithm provides a spectrum of trade-offs between accuracy and efficiency,with one balanced option finding the lowest energy configuration 87.36%of the time,while achieving a~2000×speedup in *** standardize benchmarking,we introduce the Open catalyst Dense dataset containing nearly 1000 diverse surfaces and~100,000 unique configurations.

关键词： adsorption configuration energy

Understanding the mechanism of capacity increase during early cycling of commercial NMc/graphite lithium-ion batteries

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Journal of Energy chemistry 2022年第11期31卷 34-44,I0003页

作者： Jia Guo Yaqi Li Jinhao Meng Kjeld pedersen Leonid Gurevich Daniel-Ioan Stroe AAU Energy Aalborg UniversityAalborg9220Denmark Department of Materials and Production Aalborg UniversityAalborg 9220Denmark college of Electrical Engineering Sichuan UniversityChengdu 610065SichuanChina

A capacity increase is often observed in the early stage of Li-ion battery *** study explores the phenomena involved in the capacity increase from the full cell,electrodes,and materials perspective through a combination of non-destructive diagnostic methods in a full cell and post-mortem analysis in a coin *** results show an increase of 1%initial capacity for the battery aged at 100%depth of discharge(DOD)and 45℃.Furthermore,large DODs or high temperatures accelerate the capacity *** the incremental capacity and differential voltage(Ic-DV)analysis,we concluded that the increased capacity in a full cell originates from the graphite ***,graphite/Li coin cells show an increased capacity for larger DODs and a decreased capacity for lower DODs,thus in agreement with the full cell ***-mortem analysis results show that a larger DOD enlarges the graphite dspace and separates the graphite layer structure,facilitating the Li+diffusion,hence increasing the battery capacity.

关键词： capacity increasing Lithium-ion battery Full cell coin cell Graphite anode

NMR diffusion analysis of catalytic conversion mixtures from lignocellulose biomass using PSYcHE-iDOSY

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Green Energy & Environment 2023年第5期8卷 1409-1416页

作者： Qi Zhao christian Marcus pedersen Jiamin Wang Rui Liu Yuanli Zhang Xiuyin Yan Zhenzhou Zhang Xianglin Hou Yingxiong Wang Shanxi Engineering Research center of Biorefinery Institute of Coal ChemistryChinese Academy of Sciences27 South Taoyuan RoadTaiyuan030001China center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing100049China Department of chemistry University of CopenhagenUniversitetsparken 5DK-2100CopenhagenDenmark School of chemical Engineering Zhengzhou University Zhengzhou450001China Shanxi Inspection and Testing center 106 Changzhi RoadTaiyuan030012China

The component analysis and structure characterization of complex mixtures of biomass conversion remain a challenging ***,developing effective and easy to use techniques is ***-ordered NMR spectroscopy(DOSY)is a non-selective and non-invasive method capable of achieving pseudo-separation and structure assignments of individual compounds from biomass mixtures by providing diffusion coefficients(D)of the ***,the conventional 1H DOSY NMR is limited by crowded resonances when analyzing complex mixtures containing similar chemical structure resulting in similar *** we describe the application of an advanced diffusion NMR method,Pure Shift Yielded by cHirp Excitation DOSY(PSYcHE-iDOSY),which can record high-resolution signal diffusion spectra efficiently separating compounds in model and genuine mixture samples from cellulose,hemicellulose and *** sets of isomers(D-glucose/D-fructose/D-mannose and 1,2-/1,5-pentadiol),homologous compounds(ethylene glycol and 1,2-propylene glycol),model compounds of lignin,and a genuine reaction system(furfuryl alcohol hydrogenolysis with ring opening)were successfully separated in the diffusion *** results show that the ultrahigh-resolution DOSY technique is capable of detecting and pseudo-separating the mixture components of c_(5)/c_(6) sugar conversion products and its derivative hydrogenation/hydrogenolysis from lignocellulose biomass.

关键词： Diffusion NMR Pure shift spectroscopy PSYcHE-iDOSY Mixture analysis Biomass

液体发酵饲料对生长育肥猪的能量、干物质、蛋白质以及磷消化率的影响

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饲料与畜牧（新饲料） 2015年第11期 25-27页

作者：赵伟 c.pedersen H.H.Stein 希杰(沈阳)饲料有限公司辽宁沈阳110000

文章中的两次试验通过生长育肥猪饲喂干湿料以及发酵饲料来比较相同日粮不同处理条件下的干物质(DM)、总能(GE)和粗蛋白(cP)的表观回肠消化率(AID),以及DM、GE和P的表观总肠道消化率(ATTD)。两次试验的基础日粮组成为玉米(728g/kg)、豆... 详细信息

文章中的两次试验通过生长育肥猪饲喂干湿料以及发酵饲料来比较相同日粮不同处理条件下的干物质(DM)、总能(GE)和粗蛋白(cP)的表观回肠消化率(AID),以及DM、GE和P的表观总肠道消化率(ATTD)。两次试验的基础日粮组成为玉米(728g/kg)、豆粕(240g/kg)、大豆油(10g/kg)以及维生素和矿物元素预混料。试验1中,3组试验分别饲喂干饲料日粮,以及两种干湿混合的日粮(干湿比为1∶1和1∶3),且两种干湿日粮均是饲喂前立即混合。试验2中,3组试验分别饲喂干饲料日粮以及两种发酵日粮,发酵处理在饲喂前24h,且干湿比为1∶3混合,两组发酵饲料的区别在于发酵桶中分别剩余10%(F10)或者50%(F50)的发酵饲料时,再向其内添加固定比例的饲料和水。试验1结果显示,干湿比为1∶3时,有降低日粮DM、GE和cP的AID趋势(PcP的AID,但是其对DM和GE的AID无显著影响。F50处理水平的发酵饲料相对于F10和干饲料,有增加日粮DM ATTD的趋势,但是两种处理水平的发酵饲料对日粮中GE和P的ATTD均无显著影响。

关键词：发酵饲料液态饲料猪