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Small molecule inhibitor DDQ-treated hippocampal neuronal cells show improved neurite outgrowth and synaptic branching

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Neural Regeneration Research 2025年第9期20卷 2624-2632页

作者： jangampalli adi pradeepkiran Priyanka Rawat Arubala P.Reddy Erika Orlov PHemachandra Reddy Department of Internal Medicine Texas Tech University Health Sciences CenterLubbockTXUSA Department of Nutritional Sciences College of Human SciencesTexas Tech UniversityLubbockTXUSA Department of Pharmacology&Neuroscience Texas Tech University Health Sciences CenterLubbockTXUSA Department of Neurology Texas Tech University Health Sciences CenterLubbockTXUSA Department of Speech Language and Hearing SciencesTexas Tech University Health Sciences CenterLubbockTXUSA Department of Public Health Texas Tech University Health Sciences CenterLubbockTXUSA

The process of neurite outgrowth and branching is a crucial aspect of neuronal development and *** and dendrites,sometimes referred to as neurites,are extensions of a neuron's cellular body that are used to start *** we explored the effects of diethyl(3,4-dihydroxyphenethylamino)(quinolin-4-yl)methylphosphonate(DDQ)on neurite developmental features in HT22 neuronal *** this work,we examined the protective effects of DDQ on neuronal processes and synaptic outgrowth in differentiated HT22cells expressing mutant Tau(mTau)*** investigate DDQ chara cteristics,cell viability,biochemical,molecular,western blotting,and immunocytochemistry were *** outgrowth is evaluated through the segmentation and measurement of neural *** neural processes can be seen and measured with a fluorescence microscope by manually tracing and measuring the length of the neurite *** neuronal processes can be observed and quantified with a fluorescent microscope by manually tracing and measuring the length of the neuronal ***-treated mTau-HT22 cells(HT22 cells transfected with cDNA mutant Tau)were seen to display increased levels of synaptophysin,MAP-2,andβ-***,we confirmed and noted reduced levels of both total and p-Tau,as well as elevated levels of microtubule-associated protein 2,β-tubulin,synaptophysin,vesicular acetylcholine transporter,and the mitochondrial biogenesis protein-pe roxisome prolife rator-activated receptor-gamma coactivator-1α.In mTa u-expressed HT22 neurons,we observed DDQ enhanced the neurite characteristics and improved neurite development through increased synaptic *** findings conclude that mTa u-HT22(Alzheimer's disease)cells treated with DDQ have functional neurite developmental chara *** key finding is that,in mTa u-HT22 cells,DDQ preserves neuronal structure and may even enhance nerve development function with mTa u inhibition.

关键词： diethyl(3 4-dihydroxyphenethylamino)(quinolin-4-yl)methylphosphonate(DDQ) hippocampal neuronal cells HT22 neurite outgrowth neuronal development small molecule

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Trigonella foenum-graecum seeds extract plays a beneficial role on brain antioxidant and oxidative status in alloxan-induced Wistar rats

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Food Quality and Safety 2020年第2期4卷 83-89页

作者： jangampalli adi pradeepkiran Venkata Subbaiah Nandyala Matcha Bhaskar Department of Zoology Division of Animal BiotechnologySri Venkateswara UniversityTirupatiIndia Department of Internal Medicine Texas Tech University of Health Science CentreLubbockUSA

Context:Trigonella foenum-graecum(TriFG)exhibits increased scavenger enzymatic activities and reduces the production of reactive oxygen species in diabetic ***:The present study was aimed to investigate the effect of TriFG on lipid peroxidation levels and antioxidant status in brain tissue of rats exposed to *** and Methods:Healthy male rats(180±10 g)were allocated into five *** in group 1 maintained on normal tap water served as controls and rats in groups 2,3,4,and 5 were treated as experimental *** in group 2 were intraperitoneally injected with alloxan(120 mg/kg BW)and treated as diabetic rats,whereas rats in groups 3 and 4 were maintained on same experimental regimen as that of rats in groups 1 and 2,respectively,and in addition,they were orally gavaged with herbal extracts of TriFG(0.25 g/kg BW).Diabetic rats treated with glibenclamide in group 5 were used as positive *** and Discussion:Significant(P<0.001)increase in the antioxidant enzymes with a significant(P<0.001)decrease in the lipid peroxidation levels were observed in the brain tissue of diabetic rats treated with TriFG extract as compared to diabetic and glibenclamide-treated *** significant changes were observed in pro-and antioxidant levels in brain tissue of rats treated with TriFG extract alone when compared to normal *** diabetic rats,brain mitochondrial and cytosolic enzymes like succinate dehydrogenase,glutamate dehydrogenase,and glucose-6-phosphate dehydrogenase activity levels were significantly(P<0.05)decreased with reversely increased was observed in lactate dehydrogenase activity(P<0.05).Conclusions:The findings of the present study suggested that TriFG,through its antioxidant properties,protects brain tissue by mitigating oxidative stress induced by alloxan-exposed *** extract significantly increased the antioxidant and oxidative properties in diabetic rats when compared with the control group rats.

关键词： Trigonella foenum-graecum hyperglycaemia brain antioxidants rats

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Analysis of aflatoxin B1 in contaminated feed,media,and serum samples of Cyprinus carpio *** high-performance liquid chromatography

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Food Quality and Safety 2018年第4期2卷 199-204页

作者： pradeepkiran jangampalli adi Bhaskar Matcha Division of Animal Biotechnology Department of ZoologySri Venkateswara UniversityTirupati 517502India Garrison Institute on Aging Texas Tech University of Health Science CentreLubbockUSA

Objectives:Enzyme-linked immunosorbent assay(ELISA)-,thin-layer chromatography(TLC)-,and highperformance liquid chromatography(HPLC)-sensitive methods were used for the identification of aflatoxin B1(AFB1)contaminated fish feed,media,and fish serum *** and Methods:The ELISA,TLC,and HPLC methods were validated by the quantitative and qualitative determination of AFB1 in contaminated fish feed,media,and fish blood serum ***:The primary identification of AFB1 was carried out with a DOA-ELISA test kit ELISA followed by TLC with RF values 0.81,0.79,0.81,and 0.80 of AFB1-contaminated fish feed,media,and serum samples,respectively,compared with AFB1 *** results show that the AFB1 levels in contaminated fish feed,media,and serum samples were 2.6,2.6,and 2.7 ng/mL,concentrations *** level of concentrations of AFB1 was almost similar in all three samples,but slightly higher in the fish serum sample with,2.7 ng/***,the present method is strongly recommended for monitoring AFB1 contamination in feed stuffs,especially in fisheries where the feed is under continuous exposure to ***:This method is accurate and more sensitive when compared with routine conventional AFB1 detection methods,and is highly applicable in aquaculture and fisheries to screen the mycotoxins in fish feed.

关键词： enzyme-linked immunosorbent assay TLC HPLC aflatoxin B1 analysis

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