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Effects of Ginkgo biloba extract on cell proliferation and cytotoxicity in human hepatocellular carcinoma cells

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World Journal of Gastroenterology 2004年第1期10卷 37-41页

作者： jane CJ Chao School of Nutrition and Health Sciences Taipei Medical UniversityTaipei 110TaiwanChina

AIM: To study the effect of Ginkgo biloba extract (EGb 761)containing 22-27% flavonoids (ginkgo-flavone glycosides) and 5-7% terpenoids (ginkgolides and bilobalides) on cell proliferation and cytotoxicity in human hepatocellular carcinoma (HCC) cells. METHODS: Human HCC cell lines (HepG2 and Hep3B) were incubated with various concentrations (0-1 000 mg/L) of EGb 761 solution. After 24 h incubation, cell proliferation andcytotoxicity were determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and lactate dehydrogenase (LDH) release, respectively. After 48 h incubation, the expression of proliferating cell nuclear antigen (PCNA) and p53 protein was measured by Western blotting. RESULTS: The results showed that EGb 761 (50-1 000 mg/L)significantly suppressed cell proliferation and increased LDH release (Py. LDH release of HepG2 cells without and with EGb 761 (1 000 mg/L) treatment was 6.7% and 37.7%, respectively, and that of Hep3B cells without and with EGb 761 (1 000 rag/L) treatment was 7.2% and 40.3%, respectively. The expression of PCNA and p53 protein in HepG2 cells treated with EGb 761 (1 000 mg/L) was 85% and 174% of the control group, respectively. CONCLUSION: Ginkgo bilobaextract significantly can suppress proliferation and increase cytotoxicity in HepG2 and Hep3B cells. Additionally, Ginkgo biloba extract can decrease PCNA and increase p53 expression in HepG2 cells.

关键词：银杏提取物细胞增殖细胞毒性肝细胞癌增殖细胞核抗原 p53蛋白药理作用

The splicing factor Prp31 is essential for photoreceptor development in Drosophila

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Protein & Cell 2010年第3期1卷 267-274页

作者： Payal Ray Xiaoyan Luo Elizabeth J.Rao Amina Basha Elvin A.Woodruff III jane y.Wu Department of Neurology Northwestern University Feinberg Medical SchoolChicagoIL 60611USA Magnify Inc. 2316 Riverside DrNashvilleTN 37216USA Present address:Trumbull College Yale UniversityNew HavenCT 06511USA

Retinitis pigmentosa is a leading cause of blindness and a progressive retinal disorder,affecting millions of people *** disease is characterized by photoreceptor degeneration,eventually leading to complete *** dominant(adRP)has been associated with mutations in at least four ubiquitously expressed genes encoding pre-mRNA splicing factors—Prp3,Prp8,Prp31 and *** function of adRPassociated splicing factor genes and molecular mechanisms by which mutations in these genes cause cell-type specific photoreceptor degeneration in humans remain to be *** investigate the in vivo function of these adRP-associated splicing factor genes,we examined Drosophila in which expression of fly Prp31 homolog was *** analyses show that CG6876 is the likely candidate of Drosophila melanogaster Prp31 homolog(DmPrp31).Predicted peptide sequence for CG6876 shows 57%similarity to the Homo sapiens Prp31 protein(HsPrp31).Reduction of the endogenous Prp31 by RNAi-mediated knockdown speci-fically in the eye leads to reduction of eye size or complete absence of eyes with remarkable features of photoreceptor degeneration and recapitulates the bimodal expressivity of human Prp31 mutations in adRP *** transgenic DmPrp31RNAi flies provide a useful tool for identifying genetic modifiers or interacting genes for *** of the human Prp31 in these animals leads to a partial rescue of the eye *** results indicate that the Drosophila CG6876 is the fly ortholog of mammalian Prp31 gene.

关键词： Drosophila photoreceptor degeneration retinitis pigmentosa Prp31

MiR-130a regulates and dendritic spine MeCP2 neurite outgrowth density by targeting

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Protein & Cell 2016年第7期7卷 489-500页

作者： yunjia Zhang Mengmeng Chen Zilong Qiu Keping Hu Warren McGee Xiaoping Chen Jianghong Liu Li Zhu jane y. Wu State Key Laboratory for Brain & Cognitive Science Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Laboratory of Molecular Basis of Neural Plasticity Institute of Neuroscience Chinese Academy of Sciences Shanghai 200031 China Institute of Medicinal Plant Development (IMPLAD) Chinese Academy of Medical Sciences Beijing 100193 China Department of Neurology Center for Genetic Medicine Lurie Cancer Center Northwestern University Feinberg School of Medicine Chicago IL 60611 USA

MicroRNAs （miRNAs） are critical for both development and function of the central nervous system. Significant evidence suggests that abnormal expression of miRNAs is associated with neurodevelopmental disorders. MeCP2 protein is an epigenetic regulator repressing or activating gene transcription by binding to methylated DNA. Both loss-of-function and gain-of-function muta- tions in the MECP2 gene lead to neurodevelopmental disorders such as Rett syndrome, autism and MECP2 duplication syndrome. In this study, we demonstrate that miR-130a inhibits neurite outgrowth and reduces dendritic spine density as well as dendritic complexity. Bioinformatics analyses, cell cultures and biochemical experiments indicate that miR-130a targets MECP2 and down-regulates MeCP2 protein expression. Further- more, expression of the wild-type MeCP2, but not a loss- of-function mutant, rescues the miR-130a-induced phe- notype. Our study uncovers the MECP2 gene as a pre- vious unknown target for miR-130a, supporting that miR-130a may play a role in neurodevelopment by reg- ulating MeCP2. Together with data from other groups,our work suggests that a feedback regulatory mecha- nism involving both miR-130a and MeCP2 may serve to ensure their appropriate expression and function in neural development.

关键词： miR-130a MECP2 neurite outgrowth,dendritic spines dendrite morphology

USP33, a new player in lung cancer, mediates Slit-Robo signaling

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Protein & Cell 2014年第9期5卷 704-713页

作者： Pushuai Wen Ruirui Kong Jianghong Liu Li Zhu Xiaoping Chen Xiaofei Li yongzhan Nie Kaichun WU jane y. Wu State Key Laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Department of Neurology Center for Genetic Medicine Lurie Cancer Center Northwestern University Feinberg School of Medicine 303 E. Chicago Ave. Chicago IL 60611 USA Department of Thoracic Surgery Tangdu Hospital Fourth Military Medical University Xi'an 710038 China State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases Fourth Military Medical University Xi'an 710032 China University of Chinese Academy of Sciences Beijing 100049 China Department of Pathophysiology Liaoning Medical University Jinzhou 121001 China

Ubiquitin specific protease 33 （USP33） is a multifunctional protein regulating diverse cellular processes. The expression and role of USP33 in lung cancer remain unexplored. In this study, we show that USP33 is down-regulated in multi- ple cohorts of lung cancer patients and that low expression of USP33 is associated with poor prognosis. USP33 medi- ates Slit-Robo signaling in lung cancer cell migration. Downregulation of USP33 reduces the protein stability of Robol in lung cancer cells, providing a previously unknown mechanism for USP33 function in mediating Slit activity in lung cancer ceils. Taken together, USP33 is a new player in lung cancer that regulates Slit-Robo signaling. Our data suggest that USP33 may be a candidate tumor suppressor for lung cancer with potential as a prognostic marker.

关键词： USP33 Slit Robo lung cancer Slit-Robosignaling tumor suppressor prognostic marker

Spliceosomal genes in the *** genome:a comparison with those in ***,***,*** and ***

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Protein & Cell 2011年第5期2卷 395-409页

作者： Bing yu Petra Fey Karen E.Kestin-Pilcher Alexei Fedorov Ashwin Prakash Rex L.Chisholm jane y.Wu Department of Molecular and Clinical Genetics Royal Prince Alfred Hospital and Sydney Medical School(Central)the University of SydneyNSW 2006Australia dictyBase Center for Genetic MedicineNorthwestern UniversityChicagoIL 60611USA Department of Neurology and Lurie Comprehensive Cancer Center Center for Genetic MedicineNorthwestern University Feinberg Medical SchoolChicagoIL 60611USA Department of Medicine and Program in Bioinformatics and Proteomics/Genomics The University of ToledoToledoOH 43614USA

Little is known about pre-mRNA splicing in Dictyostelium discoideum although its genome has been completely *** analysis suggests that pre-mRNA splicing plays an important role in *** gene expression as two thirds of its genes contain at least one *** curation of the genome to date has revealed 40 genes in *** with clear evidence of alternative splicing,supporting the existence of alternative splicing in this unicellular *** identified 160 candidate U2-type spliceosomal proteins and related factors in *** based on 264 known human genes involved in *** small ribonucleoproteins(snRNPs),PRP19 complex proteins and late-acting proteins are highly conserved in *** and throughout the *** non-snRNP and hnRNP families,*** orthologs are closer to those in ***,*** and *** than to their counterparts in *** splicing regulators,including SR proteins and CUGbinding proteins,were found in ***,but not in *** comprehensive catalog of spliceosomal proteins provides useful information for future studies of splicing in *** where the efficient genetic and biochemical manipulation will also further our general understanding of pre-mRNA splicing.

关键词： pre-mRNA splicing spliceosomal genes Dictyostelium discoideum comparative genomics splicing regulators

Progranulin promotes neurite outgrowth and neuronal differentiation by regulating GSK-3β

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Protein & Cell 2010年第6期1卷 552-562页

作者： Xue Gao Alvin P.Joselin Lei Wang Amar Kar Payal Ray Andrew Bateman Alison M.Goate jane y.Wu Department of Neurology Lurie Cancer CenterCenter for Genetic Medicine Northwestern University Feinberg School of MedicineChicagoIL 60611USA Division of Experimental Medicine McGill Universityand Endocrine Research LaboratoryRoyal Victoria HospitalMontréalQuébecCanada Department of Psychiatry Washington University School of MedicineSt.LouisMO 63110USA Present address:Cellular and Molecular Medicine University of OttawaOntarioCanada

Progranulin(PGRN)has recently emerged as a key player in a subset of frontotemporal dementias(FTD).Numerous mutations in the progranulin gene have been identified in patients with familial or sporadic frontotemporal lobar degeneration(FTLD).In order to understand the molecular mechanisms by which PGRN deficiency leads to FTLD,we examined activity of PGRN in mouse cortical and hippocampal neurons and in human neuroblastoma SH-Sy5y *** of mouse neurons with PGRN protein resulted in an increase in neurite outgrowth,supporting the role of PGRN as a neurotrophic *** treatment stimulated phosphorylation of glycogen synthase kinase-3 beta(GSK-3β)in cultured *** of PGRN in SH-Sy5y cells impaired retinoic acid induced differentiation and reduced the level of phosphorylated GSK-3β.PGRN knockdown cells were also more sensitized to staurosporineinduced *** results reveal an important role of PGRN in neurite outgrowth and involvement of GSK-3βin mediating PGRN *** of GSK-3βactivation as a downstream event for PGRN signaling provides a mechanistic explanation for PGRN activity in the nervous *** work also suggest that loss of axonal growth stimulation during neural injury repair or deficits in axonal repair may contribute to neuronal damage or axonal loss in FTLD associated with PGRN ***,our study suggests that modulating GSK-3βor similar signaling events may provide therapeutic benefits for FTLD cases associated with PGRN mutations.

关键词： progranulin frontotemporal lobar degeneration glycogen synthase kinase 3 beta(GSK-3β) neurite outgrowth

与神经退行性疾病相关的RNA结合蛋白在线粒体损伤中的作用

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生物化学与生物物理进展 2016年第4期43卷 374-382页

作者：朱笠邓健文王鹏刘江红 jane y.Wu 中国科学院生物物理研究所脑与认知科学国家重点实验室北京100101 中国科学院大学北京100049 Department of Neurology Center for Genetic MedicineLurie Cancer CenterNorthwestern University Feinberg School of MedicineChicagoIL 60611USA

线粒体是细胞内制造能量的细胞器,它还负责各种细胞信号的整合,参与协调多种复杂的细胞功能.线粒体是动态变化的,连续不断地进行分裂与融合,这是其功能维持和增殖遗传的关键.在过去20年中,参与线粒体分裂与融合的核心因子陆续被发现,它... 详细信息

线粒体是细胞内制造能量的细胞器,它还负责各种细胞信号的整合,参与协调多种复杂的细胞功能.线粒体是动态变化的,连续不断地进行分裂与融合,这是其功能维持和增殖遗传的关键.在过去20年中,参与线粒体分裂与融合的核心因子陆续被发现,它们在进化上高度保守,但是在形成分裂与融合复合物中的详细分子机制还有待于深入研究.线粒体分裂与融合的动态变化,是线粒体质量控制的重要组成部分,其动态平衡在细胞发育和稳态维持中起重要作用.线粒体动态变化失衡和功能失调,则会导致多种神经退行性疾病的发生.这些研究的发现为探索线粒体生物学及与疾病的关系开拓了令人振奋的新方向.

关键词： RNA结合蛋白(RBPs) 呼吸链复合物线粒体融合与分裂线粒体损伤神经退行性疾病

Interaction between climatic, environmental, and demographic factors on cholera outbreaks in Kenya

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Infectious Diseases of Poverty 2014年第1期3卷 338-346页

作者： James D Stoltzfus jane y Carter Muge Akpinar-Elci Martin Matu Victoria Kimotho Mark J Giganti Daniel Langat Omur Cinar Elci School of Medicine Department of Public Health and Preventive MedicineSt.George’s University(SGU)West IndiesGrenada African Medical and Research Foundation(AMREF) NairobiKenya. Center for Global Health College of Health SciencesOld Dominion UniversityNorfolkVAUSA

Background:Cholera remains an important public health concern in developing countries including Kenya where 11,769 cases and 274 deaths were reported in 2009 according to the World Health Organization(WHO).This ecological study investigates the impact of various climatic,environmental,and demographic variables on the spatial distribution of cholera cases in ***:District-level data was gathered from Kenya’s Division of Disease Surveillance and Response,the Meteorological Department,and the National Bureau of *** data included the entire population of Kenya from 1999 to ***:Multivariate analyses showed that districts had an increased risk of cholera outbreaks when a greater proportion of the population lived more than five kilometers from a health facility(RR:1.025 per 1%increase;95%CI:1.010,1.039),bordered a body of water(RR:5.5;95%CI:2.472,12.404),experienced increased rainfall from October to December(RR:1.003 per 1 mm increase;95%CI:1.001,1.005),and experienced decreased rainfall from April to June(RR:0.996 per 1 mm increase;95%CI:0.992,0.999).There was no detectable association between cholera and population density,poverty,availability of piped water,waste disposal methods,rainfall from January to March,or rainfall from July to ***:Bordering a large body of water,lack of health facilities nearby,and changes in rainfall were significantly associated with an increased risk of cholera in Kenya.

关键词： Cholera Education Health infrastructure Population density Poverty Rainfall Waste disposal Water supply

Investigation of fluorescence characterization and electrochemical behavior on the catalysts of nanosized Pt-Rh/y-AI203 to oxidize gaseous ammonia

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Frontiers of Environmental Science & Engineering 2013年第3期7卷 428-434页

作者： Chang-Mao HUNG Wen-Liang LAI jane-Li LIN Department of Vehicle Engineering Yung-Ta Institute of Technology and Commerce Pingtung 909 China Department of Environmental Science and Occupational Safety and Hygiene Tajen University Pingtung 907 China

This work describes the environmentally friendly technology for oxidation of ammonia （NH3） to form nitrogen at temperatures range from 423K to 673K by selective catalytic oxidation （SCO） over a nanosized Pt- Rh/γ-A12O3 catalyst prepared by the incipient wetness impregnation method of hexachloroplatinic acid （H2PtC16） and rhodium （Ⅲ） nitrate （Rh（NO3）3） with γ-A12O3 in a tubular fixed-bed flow quartz reactor （TFBR）. The characterization of catalysts were thoroughly measured using transmission electron microscopy （TEM）, three- dimensional excitation-emission fluorescent matrix （EEFM） spectroscopy, UV-Vis absorption, dynamic light- scattering （DLS）, zeta potential meter, and cyclic voltam- metry （CV）. The results demonstrated that at a temperature of 673K and an oxygen content of4%, approximately 99% of the NH3 was removed by catalytic oxidation over the nanosized Pt-Rh/γ-A12O3 catalyst. N2 was the main product in NH3-SCO process. Further, it reveals that the oxidation of NH3 was proceeds by the over-oxidation of NH3 into NO, which was conversely reacted with the NH3 to yield N2. Therefore, the application ofnanosized Pt-Rh/γ-A12O3 catalyst can significantly enhance the catalytic activity toward NH3 oxidation. One fluorescent peak for fresh catalyst was different with that of exhausted catalyst. It indicates that EEFM spectroscopy was proven to be an appropriate and effective method to characterize the Pt clusters in intrinsic emission from nanosized Pt-Rh/γ-A12O3 catalyst. Results obtained from the CV may explain the significant catalytic activity of the catalysts.

关键词： ammonia (NH3) nanosized Pt- Rh/γ-A12O3catalyst excitation-emission fluorescent matrix (EEFM) selective catalytic oxidation (SCO) tubular fixed-bedreactor (TFBR)